2WU6


Conserved Protein Domain Family
PKc_CLK3

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cd14214: PKc_CLK3 
Click on image for an interactive view with Cn3D
Catalytic domain of the Dual-specificity protein kinase, CDC-like kinase 3
Dual-specificity PKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine as well as tyrosine residues on protein substrates. CLK3 is predominantly expressed in mature spermatozoa, and might play a role in the fertilization process. CLKs are involved in the phosphorylation and regulation of serine/arginine-rich (SR) proteins, which play a crucial role in pre-mRNA splicing by directing splice site selection. SR proteins are phosphorylated first by SR protein kinases (SRPKs) at the N-terminus, which leads to its assembly into nuclear speckles where splicing factors are stored. CLKs phosphorylate the C-terminal part of SR proteins, causing the nuclear speckles to dissolve and splicing factors to be recruited at sites of active transcription. Based on a conserved "EHLAMMERILG" signature motif which may be crucial for substrate specificity, CLKs are also referred to as LAMMER kinases. CLKs autophosphorylate at tyrosine residues and phosphorylate their substrates exclusively on serine/threonine residues. The CLK3 subfamily is part of a larger superfamily that includes the catalytic domains of other protein serine/threonine PKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.
Statistics
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PSSM-Id: 271116
Aligned: 3 rows
Threshold Bit Score: 706.773
Created: 24-Dec-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 19 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:2WU6; Human CLK3 binds inihibitor DKI; contacts at 4A
  • Comment:also based on the structures of other CMGC kinases bound with ATP or ATP analogs
  • Citation:PMID 9739089

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                            ####    #             # #                                 # 
2WU6_A        39 GHLVCRIGDWLQeRYEIVGNLGEGTFGKVVECLDHargkSQVALKIIRNvgkYREAARLEINVLKKIKEKDkenKFLCVL 118 human
NP_001012542 139 GHLVCRIGDWLQeRYEIVGSLGEGTFGKVVECVDHargkSQVALKIIKNvgkYREAARLEINVLKKIKEKDkenKFLCVL 218 chicken
NP_001093358 147 GHLVCRTGERIQeRYEIVGDLGIGTFGKVVECLDLargnSRVALKIIRNvtkYREAAQLEINVLEKIKEQDqdnKNMCVL 226 African clawed ...
Feature 1                      ####  #  #                                         ## #           
2WU6_A       119 MSDWFNFHGHMCIAFELLGKNTFEFLKENnfQPYPLPHVRHMAYQLCHALRFLHENQLTHTDLKPENILFVNSEFETLYN 198 human
NP_001012542 219 MSDWFNFHGHMCIAFELLGKNTFEFLKENnfQPYPLPQIRHMAYQLCHALKFLHDNQLTHTDLKPENILFVNSDFDTLYN 298 chicken
NP_001093358 227 MRDWFDFHGHVCIAFELLGKSTFEFQKENnfLPYPLTHIRHMAFQLCHALKFLHENQLTHTDLKPENILFVNSEYDVCYN 306 African clawed ...
Feature 1                         ##                                                             
2WU6_A       199 ehkSCEEKSVKNTSIRVADFGSATFDHEHHTTIVATRHYRPPEVILElGWAQPCDVWSIGCILFEYYRgFTLFQTHeNRE 278 human
NP_001012542 299 ekkSCEEKSIRNTSIRVADFGSATFDHEHHTTIVATRHYRPPEVILElGWAQPCDVWSTGCILFEYYRgFTLFQTHeNRE 378 chicken
NP_001093358 307 eskKCEEKHVKNSSIRLVDFGSATFDHEYHTTIVATRHYRPPEVILElGWSQPCDVWSLGCILFEYYTgFTLFQTHdNRE 386 African clawed ...
Feature 1                                                                                        
2WU6_A       279 HLVMMEKILGPiPSHMIHRTrKQKYFYKGGLVWDENSSDGRYVKENCKPlksYMLQDSLEHVQLFDLMRRMLEFDPAQRI 358 human
NP_001012542 379 HLVMMEKILGPiPSHMIHRTrKQKYFYKGNLVWDENTSDGRYVQENCKPlrtYMLHDSLEHAQLFDLIRRMLEFDPSRRI 458 chicken
NP_001093358 387 HLVMIERILGPlPRRMVYKTrKQKYFHNGSLIWDENSSDGRYVSKNCHQlmsYKSGDSPEHTQLFDLLSRMLECRPALRI 466 African clawed ...
Feature 1                   
2WU6_A       359 TlAEALLHPFF 369 human
NP_001012542 459 TfSEALLHPFF 469 chicken
NP_001093358 467 TlKEALEHAFF 477 African clawed frog

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