3AJ4,2D9V


Conserved Protein Domain Family
PH_evt

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cd13265: PH_evt 
Click on image for an interactive view with Cn3D
Evectin Pleckstrin homology (PH) domain
There are 2 members of the evectin family (also called pleckstrin homology domain containing, family B): evt-1 (also called PLEKHB1) and evt-2 (also called PLEKHB2). evt-1 is specific to the nervous system, where it is expressed in photoreceptors and myelinating glia. evt-2 is widely expressed in both neural and nonneural tissues. Evectins possess a single N-terminal PH domain and a C-terminal hydrophobic region. evt-1 is thought to function as a mediator of post-Golgi trafficking in cells that produce large membrane-rich organelles. It is a candidate gene for the inherited human retinopathy autosomal dominant familial exudative vitreoretinopathy and a susceptibility gene for multiple sclerosis. evt-2 is essential for retrograde endosomal membrane transport from the plasma membrane (PM) to the Golgi. Two membrane trafficking pathways pass through recycling endosomes: a recycling pathway and a retrograde pathway that links the PM to the Golgi/ER. Its PH domain that is unique in that it specifically recognizes phosphatidylserine (PS), but not polyphosphoinositides. PS is an anionic phospholipid class in eukaryotic biomembranes, is highly enriched in the PM, and plays key roles in various physiological processes such as the coagulation cascade, recruitment and activation of signaling molecules, and clearance of apoptotic cells. PH domains are only found in eukaryotes. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.
Statistics
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PSSM-Id: 270085
Aligned: 15 rows
Threshold Bit Score: 152.842
Threshold Setting Gi: 350646596
Created: 3-Jan-2012
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
phosophoserine
Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:phosophoserine binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                  # ###### #             #          #                                   
3AJ4_A         3 MAFVKSGWLLRQSTILKRWKKNWFDLWSDGHLIYYDDQTRQNIEDKVHMPMDCINIRTGqeCRDTQPPDGKSKDCMLQIV 82  human
2D9V_A         6 SGLVRGGWLWRQSSILRRWKRNWFALWLDGTLGYYHDETAQDEEDRVVIHFNVRDIKVGqeCQDVQPPEGRSRDGLLTVN 85  house mouse
AAH75203       1 MALVKSGWLWRQSCVLRRWKKHWFDLWLDGNLLYYPDENRRTVENRIPMKFNCVNVRAGqeCGDILPPDGNTQESLVTVE 80  African clawed ...
CAH65344       3 MALVKSGWLWRQSSILRRWKRNWFVLYLDGSLVYYHDETQRDMDGRIHIKYSCRDVRTGreCRDVQPPEGKSRECLLTIV 82  chicken
AAI24815      26 MALLKSGWLWRQSSVLKRWKLNWCDLWIDGSFVFYKSESRRDYETKVNLKSSCVNVRSGleCAGVGPPESHPRENLLVVY 105 zebrafish
XP_002121756   4 IPTFKAGWLLRQSSVLKRWKKNWFVLYGDGNLSFYEDESRKEKHGNFNITAECAAINTAl-ECNVDPPDHRNFGCLMKLI 82  Ciona intestinalis
XP_002593763   4 EVIAKSGWLLRQTTVLKRWKREFFVLYVDGQLAHYEDERKLEVKGMISMRKECLGIKLGgmVNDVTVPDGRSRDALLAIV 83  Florida lancelet
XP_002738943   8 EFITKAGWVHRQSDILKRWKKQWCVLYGDGGLACYTDETRREREMVIRMPIDAIFINSGlaVRSVTPPDRRGHDSLIEIR 87  Saccoglossus ko...
XP_001368508 141 MALVKAGWLWRQSTILRRWKRNWFVLWMDGTLGYYKDDSLQEEEERVLIRYNCREVKTGeeCPEMQLPEGQTRDGLLIVF 220 gray short-tail...
XP_797068     10 HHVVKAGWMQRETDVLKRWKHCFCILKTDGILSYYSDEKQKDLQGHINLPRDCFVLKVArdVSGVTPPSSHDVMGLIEIR 89  purple urchin
Feature 1              #                     
3AJ4_A        83 CRDGkTISLCAEsTDDCLAWKFTLQDSR 110 human
2D9V_A        86 LREGsRLHLCAEtRDDAIAWKTALMEAN 113 house mouse
AAH75203      81 LRDRsKLLLCAEsEDDAVAWKMALMDTQ 108 African clawed frog
CAH65344      83 LRDGsKTTLCAEsEDDAIAWKMAVLEAK 110 chicken
AAI24815     106 LRDGtMLSLCANsEDEALAWKLTLMEAK 133 zebrafish
XP_002121756  83 SRHNnNLVLCAAnSDEALSWKMMVEQMI 110 Ciona intestinalis
XP_002593763  84 TRTT-AMFVCADnADDAAVWKFALEEAK 110 Florida lancelet
XP_002738943  88 THLT-SINLCAEsPDDCKAWTIALEQMR 114 Saccoglossus kowalevskii
XP_001368508 221 LRDGpRLTLCADsKDEALHVMGDNSKLC 248 gray short-tailed opossum
XP_797068     90 SSNS-ATIFCAEsYDDAQMWKLVFDDVI 116 purple urchin

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