1KTB,3A21,1UAS,3LRK,1SZN,3HG3,3CC1,3A5V,4NZJ


Conserved Protein Domain Family
GH27

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cd14792: GH27 
Click on image for an interactive view with Cn3D
glycosyl hydrolase family 27 (GH27)
GH27 enzymes occur in eukaryotes, prokaryotes, and archaea with a wide range of hydrolytic activities, including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-N-acetylgalactosaminidase, and 3-alpha-isomalto-dextranase. All GH27 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH27 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
Statistics
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PSSM-Id: 269893
Aligned: 196 rows
Threshold Bit Score: 181.214
Created: 25-Sep-2013
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitecatalytic site
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:3HG3; human alpha-galactosidase (D170 mutant) with bound substrate, contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1               #                                                   ##                   
1KTB_A         9 PPMGWLAWErfrcnvncre------dprqcisemLFMEMADRIAEdgw-relGYKYINIDDCWaakq-----rdaeGRLV 76  chicken
3HG3_A         9 PTMGWLHWErfmcnldcqe------epdscisekLFMEMAELMVSegw-kdaGYEYLCIDDCWmapq-----rdseGRLQ 76  human
WP_006382298  43 PAMGWSSWSfvrrt----------------pteaKMKAQADALVSsgl-kdhGYVYVNLDDFWqkcddngfvvdsaGRWT 105 Streptomyces tu...
YP_003116947  46 PLMGWSSWSflrsa----------------pteaKMKAQAQSMSSsgl-vaaGYKYVNLDDFYylnpgt--tvdsyGRWV 106 Catenulispora a...
EMR61358      43 PSRGWNSFGiqanpstd---------psftfdqaGVTTQCDVLSTntalkasGYVYCSLDSGWsvggn----gddfGRII 109 Eutypa lata UCREL1
EJT98168       9 PPRGWNSFGmqvgirsa---------thfamtqnHTIAQCDLLADpdalggaGYEYCSLDSGWsigdh----gdehGRIM 75  Dacryopinax sp....
EGG11933      39 GYRGWSTWSlqaykgtgy--------gfdwanekNVKAQADVMASefs--alGYDRINIDSGWgdav-----ldrfGRMQ 103 Melampsora lari...
EGF97786      36 TFRGWTSWSlqafkgpgy--------gadwfkeeNIKRQADVLGNefk--qaGFGYFNLDSGWqdek-----ldefGRVQ 100 Melampsora lari...
EPB90919      27 PVLGFSTWSsqllddvpgyg---gkhvnpwfneqQIKDISDTMREkm----pSYEYINLDSGWcnec------deyGRWD 93  Mucor circinell...
EED14333     185 SARGWNTYGiqalsngsevvpsfvgssglnylqeFVKTQCGVLAQsgf-qqaGYVYCSLDSGWqtqt-----tdeyGRIV 258 Talaromyces sti...
Feature 1                                  #                                                     
1KTB_A        77 PDPERFPrgikALADYVHarGLKLGIYGDLGRltc--------------------------------------------- 111 chicken
3HG3_A        77 ADPQRFPhgirQLANYVHskGLKLGIYADVGNktc--------------------------------------------- 111 human
WP_006382298 106 VDAAKFPggikALADYIHakGLKFGFYVTPGIarnavlkntpiegtsyhakdi--------------adtskteknynck 171 Streptomyces tu...
YP_003116947 107 IDTGKFPdgmaNLGSYIHslGEKFGMYLTPGIpvaaykqntpiqgtsfhaqdivsn---------tssyatnynfgngam 177 Catenulispora a...
EMR61358     110 ADSSKFDi--pSLADHLHaeGLLLGVYVVPGAfiadeaktilgtsvtig----------------------svcsgdngl 165 Eutypa lata UCREL1
EJT98168      76 YVEKQLDl--pALADHLHakGLKLGVYVVPGAfskdanktiygthirlnn---------------------tftghdngl 132 Dacryopinax sp....
EGG11933     104 LDHKKYPsgieSLSRYLSgkGLKLGLYYLPGIdsravrsksrvlatnftaneivkcagiqmssqktnvsschrpyanafk 183 Melampsora lari...
EGF97786     101 LSTVRFPsgiqSLQGYLEtrGLSLGLYYLPGIdsravdnktpvkgtkytadqivlcp------ttsnstnckrppanafs 174 Melampsora lari...
EPB90919      94 VRKDVFPnglrPIADHLKknGHKLGLYILPGIrqdgadkkktikgthhtldel---------------vpsrkegcgfkg 158 Mucor circinell...
EED14333     259 YDSTRFNl--pQLASWLHgqGLKLGVYVVPGVpcsaanktikgtnisin----------------------dalngnndq 314 Talaromyces sti...
Feature 1                                        # #                                             
1KTB_A       112 ------ggYPGTtLDRVEQDAQTFAeWGVDMLKLDGCYssg-----------keqAQGYPQMARALNAt---------gR 165 chicken
3HG3_A       112 ------agFPGS-FGYYDIDAQTFAdWGVDLLKFAGCYcdsl----------enlADGYKHMSLALNRt---------gR 165 human
WP_006382298 172 nmyyidygKPGA-QEFVNSWAKQFAsWGVDYLKIDGVGsq--------------dIPDVQAWDKALRAt---------gR 227 Streptomyces tu...
YP_003116947 178 ynidyaknPAAA-QAFLNSWANELAgYGIDYLKVDGISpndg---------daqgVADTQHWSQALNQt---------gR 238 Catenulispora a...
EMR61358     166 arcnfnysDPAV-QQWHNSVVDQFAsWGVDFIKLDFITpgspsns---vnlppdlSGEVVGYHQAIAQs---------dR 232 Eutypa lata UCREL1
EJT98168     133 sridfnytRDGV-QQWHDSVVNQFAeWGVDFIKLDFVTpgspehd---vhlspdtSGSVIAFHKAIAKa---------sR 199 Dacryopinax sp....
EGG11933     184 agyalnysHPGA-QAYVDSIVDQLYsWNVSFVKLDGNVpgssiessdkdfkacntSPDLLAWRSAIDRlhksewrnkgrE 262 Melampsora lari...
EGF97786     175 agqsldfsHPGA-QAYIDSIVDQLYsWKVTFVKYAGYIpgssvdpnnadfssatsAADLAAWRSAIDRlhqtk--ysnqP 251 Melampsora lari...
EPB90919     159 ttfmpdehNELV-QKYYDSIADLFHeWGVSFIKLDAVGpgggseyy--pftapdnRACTQMMHNAFKR-----------Y 224 Mucor circinell...
EED14333     315 lycswnfsKDGV-QEWHDSVVAQWAsWGVDMIKLDFLTpgspqng---anldcdsSDAAHAYLNAIKAsgr-----hmrL 385 Talaromyces sti...
Feature 1              #                        #   #                                            
1KTB_A       166 PIVYSCSWpayqgglppkvnytllgeicnLWRNYDDIqds--------------wdsVLSIVDWFFTNqdvlqp-----f 226 chicken
3HG3_A       166 SIVYSCEWplymwpfqkp-nyteirqycnHWRNFADIdds--------------wksIKSILDWTSFNqerivd-----v 225 human
WP_006382298 228 PINYALSNnlpiadaa------twrqlanSWRTQGDVecycgpgan---gsgypltdWSHVSARFNTAaswqph------ 292 Streptomyces tu...
YP_003116947 239 TIHLELSNsltpadaa------swqqysnGWRIDGDVecycgsn------ssfpltdWNNVSQRFTDVqpwigv------ 300 Catenulispora a...
EMR61358     233 QIRLDISWkldrs-----------eqffdIWETNADSmrtdqdinnggaatfvnwgtVQRAIDNYRQYtttvtet----s 297 Eutypa lata UCREL1
EJT98168     200 PMRLDVSWklepnvtyy----dvsrrnadSMRIDQDInnqra-----------dtfvSWATIQRAIDNcrqyinlhthsv 264 Dacryopinax sp....
EGG11933     263 RIWLTTSWalppseae------ilretvdAWRVAIDIesygk-----------emttFDRVIRNARAAarwtsve----k 321 Melampsora lari...
EGF97786     252 KIWISAAWeiaksqkd------ilkvnadSHRVAIDIaayst-----------vmttFDRVIRNARVAaswssvd----a 310 Melampsora lari...
EPB90919     225 DMWLEISWqidpayad------ewaqisnGARIYVDIesyst-----------ksmtSSHRIMQRITPcekwadtc-vvg 286 Mucor circinell...
EED14333     386 DLSWKLCRnetwlpi--------wsqlgdSMRIDQDLdnygy-----------ntfvAWATAQRAIDNyrqyiglqaqrn 446 Talaromyces sti...
Feature 1                                                                                  
1KTB_A       227 agpghWNDPDMLIIGnfgl--------syeqSRSQMALWTimaAPLLMSTDLrtispsakkILQNRLMIQINQD 292 chicken
3HG3_A       226 agpggWNDPDMLVIGnfgl--------swnqQVTQMALWAimaAPLFMSNDLrhispqakaLLQDKDVIAINQD 291 human
WP_006382298 293 agpggWNDLDSLEIGngdqvg-----ltadqRRSHFTLWAmaaSPLLLGTDLtaldsvdkaMLANDRLIGVDQD 361 Streptomyces turgidis...
YP_003116947 301 ggtggWNDLDSVEIGngsndg-----ltldeRKTQLTLWAienSNLTLGVDMthldstdvgLLTNSEVLAVDQA 369 Catenulispora acidiph...
EMR61358     298 taltiYPDMDNLFVGnaavvs----gvtddeRQTIMTHWLgaaANLIIGSDLtnlddfglnLLTNADALAVADF 367 Eutypa lata UCREL1
EJT98168     265 epltiYPDLDNLYVGnaanvs----gitdaqRQTMMTFWLgagANLLIGSDLlqlddfgkkLLTDTEALDIADF 334 Dacryopinax sp. DJM-7...
EGG11933     322 nrgpgLLDMDSIVIAnmt----------ieeCRSMITIWAltgTPFYLGDDLkrlpkerkaLMQNPAVLEIQRL 385 Melampsora larici-pop...
EGF97786     311 nrggtVLDLDAILLAdls----------aaeAKTMVTLWAllgSPIYSGDDLtklsqekkaLLTNPSVLDVAKR 374 Melampsora larici-pop...
EPB90919     287 sdygfYIDLDVVLVGmtvngkcvdgldnddvRQTCISFWAivsSVFCIGSDPraipekyleWYNHKEMLEIHQS 360 Mucor circinelloides ...
EED14333     447 vplttYPDMDNLFTAnaenlt----gvpdlqRITIMNHWLgagANLILGGDLtaidslgyqLLTSAESIAAADF 516 Talaromyces stipitatu...

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