1FLJ,1CNX,1TB0


Conserved Protein Domain Family
alpha_CA_I_II_III_XIII

?
cd03119: alpha_CA_I_II_III_XIII 
Click on image for an interactive view with Cn3D
Carbonic anhydrase alpha, isozymes I, II, and III and XIII. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. Most alpha CAs are monomeric enzymes. The zinc ion is complexed by three histidines. This vertebrate subgroup comprises isozymes I, II, and III, which are cytoplasmic enzymes. CA I, for example, is expressed in erythrocyes of many vertebrates; CA II is the most active cytosolic isozyme; while it is being expressed nearly ubiquitously, it comprises 95% of the renal carbonic anhydrase and is required for renal acidification; CA III has been implicated in protection from the damaging effect of oxidizing agents in hepatocytes. CAXIII may play important physiological roles in several organs.
Statistics
?
PSSM-Id: 239393
View PSSM: cd03119
Aligned: 10 rows
Threshold Bit Score: 508.903
Threshold Setting Gi: 4502517
Created: 1-Nov-2005
Updated: 17-Jan-2013
Structure
?
Program:
Drawing:
Aligned Rows:
 
active sitezinc binding
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1CNX: human carbonic anhydrase II binds zinc and benzenesulfonamide, contacts at 4.5 Angstrom
    View structure with Cn3D
  • Comment:zinc binding histidines and other residues found to be important for enzymatic activity

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                      #                
1FLJ_A        1 XAKEWGYASHNGPEHWHELYPIAKGDNQSPIELHTkDIRHDpSLQPWSVSYDpgsAKTILNNGKTCRVVFDDtfdrSMLR 80
1CNX          1 MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDThTAKYDpSLKPLSVSYDqatSLRILNNGHAFNVEFDDsqdkAVLK 80
gi 18858379   1 MAHAWGYGPADGPESWAESFPIANGPRQSPIDIVPtQAQHDpSLKHLKLKYDpatTKSILNNGHSFQVDFVDddnsSTLA 80
gi 13436164   1 MAKEWGYASHNGPDHWHELFPNAKGENQSPIELHTkDIRHDpSLQPWSVSYDggsAKTILNNGKTCRVVFDDtydrSMLR 80
gi 115454     1 MSHHWGYDSHNGPAHWHEHFPIANGERQSPIAISTkAARYDpALKPLSFSYDagtAKAIVNNGHSFNVEFDDssdkSVLQ 80
gi 50731674   2 AQSVWGYDKENGPEHWHQNYPMAKGDKQSPIEINSkDVRHDtSLSPWHASYDpgaAKTILNNGRTCRVVFDDtfdrSVLR 81
gi 50417999   1 MMHQWGYEDHNGPDVWQDLFPLANGDRQSPINIITkDALYDpSLQPLLVDYDphsAKVISNSGHTVAVEFDDgddsSVVR 80
gi 4502517    2 ASPDWGYDDKNGPEQWSKLYPIANGNNQSPVDIKTsETKHDtSLKPISVSYNpatAKEIINVGHSFHVNFEDndnrSVLK 81
1TB0_X        1 MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDThTAKYDpSLKPLSVSYDqatSLRILNNGHAFNVEFDDsqdkAVLK 80
gi 30580350   2 SRLSWGYREHNGPIHWKEFFPIADGDQQSPIEIKTkEVKYDsSLRPLSIKYDpssAKIISNSGHSFNVDFDDtenkSVLR 81
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                  # # #         #            #                                         
1FLJ_A       81 GGPLSGPYRLRQFHLHWGSSDDHGSEHTVDGVKYAAELHLVHWNp-KYNTFGEAlkqPDGIAVVGIFLKIGr-EKGEFQI 158
1CNX         81 GGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNt-KYGDFGKAvqqPDGLAVLGIFLKVGs-AKPGLQK 158
gi 18858379  81 GGPITGIYRLRQFHFHWGSSDDKGSEHTIAGTKFPCELHLVHWNt-KYPNFGEAaskPDGLAVVGVFLKIGa-ANPRLQK 158
gi 13436164  81 GGPLPGPYRLRQFHLHWGSSDDHGSEHTVDGVKYAAELHLVHWNp-KYNTFKEAlkqRDGIAVIGIFLKIGh-ENGEFQI 158
gi 115454    81 GGALDGVYRLVQFHIHWGSCEGQGSEHTVDGVKYDAELHIVHWNv-KYGKFAEAlkhPDGLAVVGIFMKVGn-AKPEIQK 158
gi 50731674  82 GGPLTGAYRLRQLHLHWGSADDHGSEHVIDGVKYAAELHLVHWNp-KHGNFAGAlkqPDGVAVVGVFLKVGktPKPEMKR 160
gi 50417999  81 GGPLMGNYRLRQLHFHWGPSDGHGSEHKVDGVDYAAELHIVHWNseKFSSFVKAacaPDGLAVLGVFLKVGe-PNRYIEK 159
gi 4502517   82 GGPFSDSYRLFQFHFHWGSTNEHGSEHTVDGVKYSAELHVAHWNsaKYSSLAEAaskADGLAVIGVLMKVGe-ANPKLQK 160
1TB0_X       81 GGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNt-KYGDFGKAvqqPDGLAVLGIFLKVGs-AKPGLQK 158
gi 30580350  82 GGPLTGSYRLRQVHLHWGSADDHGSEHIVDGVSYAAELHVVHWNsdKYPSFVEAahePDGLAVLGVFLQIGe-PNSQLQK 160
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                              #                                        
1FLJ_A      159 LLDALDKIKTKGKEAPFNHFDPSCLFPAcrDYWTYHGSFTTPPCEECIVWLLLKEPMTVSSDQMAKLRSLFASAE--NEP 236
1CNX        159 VVDVLDSIKTKGKSADFTNFDPRGLLPEslDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGE--GEP 236
gi 18858379 159 VLDALDDIKSKGRQTTFANFDPKTLLPAslDYWTYEGSLTTPPLLESVTWIVLKEPISVSPAQMAKFRSLLFSSE--GET 236
gi 13436164 159 FLDALDKIKTKGKEAPFTKFDPSCLFPAcrDYWTYQGSFTTPPCEECIVWLLLKEPMTVSSDQMAKLRSLLSSAE--NEP 236
gi 115454   159 VVDALNSIQTKGKQASFTNFDPTGLLPPcrDYWTYPGSLTTPPLHECVIWHVLKEPITVSSEQMCKLRGLCFSAE--NEP 236
gi 50731674 161 ILEEIDNIKTKGKEAPFQHFDPSILFPKsrDYWTYHGSFTTPPCEECITWILLREPIEVSPDQMARLRSLSKNGE--NEP 238
gi 50417999 160 ITDTFGAIRSKGKQSPFTNFDPSCLLPAsmDFWTYPGSLTVPPLLESVVWTVLKDPISISCEQLAMFRSLLSTKEtaGIE 239
gi 4502517  161 VLDALQAIKTKGKRAPFTNFDPSTLLPSslDFWTYPGSLTHPPLYESVTWIICKESISVSSEQLAQFRSLLSNVE--GDN 238
1TB0_X      159 VVDVLDSIKTKGKSADFTNFDPRGLLPEslDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGE--GEP 236
gi 30580350 161 ITDTLDSIKEKGKQTRFTNFDLLSLLPPswDYWTYPGSLTVPPLLESVTWIVLKQPINISSQQLAKFRSLLCTAE--GEA 238
                       250       260
                ....*....|....*....|...
Feature 1                              
1FLJ_A      237 PVPLVGNWRPPQPIKGRVVRASF 259
1CNX        237 EELMVDNWRPAQPLKNRQIKASF 259
gi 18858379 237 PCCMVDNYRPPQPLKGRKVRASF 259
gi 13436164 237 PVPLVSNWRPPQPINNRVVRASF 259
gi 115454   237 VCRMVDNWRPCQPLKSREVRASF 259
gi 50731674 239 MCPLVDNWRPLQPVKGRIVRASF 261
gi 50417999 240 SCCMTTNHRPVQPLKNRKVRASF 262
gi 4502517  239 AVPMQHNNRPTQPLKGRTVRASF 261
1TB0_X      237 EELMVDNWRPAQPLKNRQIKASF 259
gi 30580350 239 AAFLVSNHRPPQPLKGRKVRASF 261

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap