1EF7,1DEU


Conserved Protein Domain Family
Peptidase_C1A_CathepsinX

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cd02698: Peptidase_C1A_CathepsinX 
Click on image for an interactive view with Cn3D
Cathepsin X; the only papain-like lysosomal cysteine peptidase exhibiting carboxymonopeptidase activity. It can also act as a carboxydipeptidase, like cathepsin B, but has been shown to preferentially cleave substrates through a monopeptidyl carboxypeptidase pathway. The propeptide region of cathepsin X, the shortest among papain-like peptidases, is covalently attached to the active site cysteine in the inactive form of the enzyme. Little is known about the biological function of cathepsin X. Some studies point to a role in early tumorigenesis. A more recent study indicates that cathepsin X expression is restricted to immune cells suggesting a role in phagocytosis and the regulation of the immune response.
Statistics
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PSSM-Id: 239149
View PSSM: cd02698
Aligned: 12 rows
Threshold Bit Score: 353.257
Threshold Setting Gi: 47028329
Created: 2-Jun-2005
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:The catalytic residues of C1 family peptidases are Cys and His, forming a catalytic dyad. Two other residues play an important role in catalysis: a Gln preceding the catalytic Cys, believed to help in the formation of the oxyanion hole; and an Asn residue which orients the imidazolium ring of the catalytic His.
  • Citation:PMID 8439290
  • Citation:PMID 7470479
  • Citation:PMID 8416808
  • Structure:1DEU; human procathepsin X; contacts at 3.5A between active site and inhibitor propeptide
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                            #        #                                                 
1EF7_A        1 LPKSWDWRNVdgvnYASITRNQHIPQYCGSCWAHASTSAMADRINIKRKGA-WPSTLLSVQNVIDCGN--AGSCEGGNDL 77
1DEU_A       36 LPKSWDWRNVdgvnYASITRNQHIPQYCGSCWAHASTSAMADRINIKRKGA-WPSTLLSVQNVIDCGN--AGSCEGGNDL 112
gi 47939805  53 LPKAWDWRNVngtnYVSTTRNQHIPQYCGSCWAHGSTSAMADRINIKRNGV-WPSSYLSVQHVIDCAD--AGSCEGGDHG 129
gi 50758927 176 LPQSWDWRNVngvnYASTTRNQHIPQYCGSCWAHGSTSALADRINIKRKGA-WPSAYLSVQNVIDCAN--AGSCEGGDHT 252
gi 17507081 186 LPKTWDWRDAnginYASADRNQHIPQYCGSCWAFGATSALADRINIKRKNA-WPQAYLSVQEVIDCSGa-GTCVMGGEPG 263
gi 53734636  54 LPKEWDWRNIkgvnYVSTTRNQHIPQYCGSCWAHGSTSALADRINIKRKAA-WPSAYLSVQNVIDCGD--AGSCSGGDHS 130
gi 60467666  58 VPQSWDWRNVsgvnYLTMNRNQHIPQYCGGCWAFASTSSISDRIKIQRKAA-FPDVNVAPQHLIDCNG--GGTCDGGDPG 134
gi 50657031  55 IPDSFDWRDVnntyFVSPVTNQFLPSPCGCCWAHAAVGALTDRMMIATQAK-RSIVPLSPQVLLDCADpdLGSCHGGSAL 133
gi 29150712  50 MPKSFDWRENa---YLSSVKNQHLPTYCGSCWAFASTSTIADRIYIAKNLShFDHFSLSVQVVIACAQs-GDCKLGGFAS 125
gi 47028329  56 VPDELNWCDMdgvsYCTRSRNQHIPQYCGSCWAHGSVSALGDRIKIARKAQ-GADIDLSVQHILNCGG--VGSCHGGSVA 132
gi 17562680 221 LPTGWDWRNVsgvnYCSPTRNQHIPVYCGSCWVFGTTGALNDRFNVARKGR-WPMTQLSPQEIIDCNG--KGNCQGGEIG 297
gi 60469326  49 LPTQYDWRNIsgssYITITRNQHLPQYCGSCWAHGTTSALGDRIKIGRKGT-FPEVVLAPQVLLNCAGp-DNTCDGGDPT 126
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
1EF7_A       78 SVWDYAHQ-----HGIPDETCNNYQA------KDQECDkfNQCGTCNEf------------KECHAIrnyTLWRVGDYGS 134
1DEU_A      113 SVWDYAHQ-----HGIPDETCNNYQA------KDQECDkfNQCGTCNEf------------KECHAIrnyTLWRVGDYGS 169
gi 47939805 130 GVWEYAHS-----HGIPDETCNNYQA------RDQKCDkfNQCGTCVTf------------GKCFNLsnyTLWKVGDFGS 186
gi 50758927 253 GVWMYAHD-----HGIPDETCNNYQA------KNQKCKkfNQCGTCVTf------------GECHVIknyTLWKVADYGA 309
gi 17507081 264 GVYKYAHE-----HGIPHETCNNYQA------RDGKCDpyNRCGSCWP-------------GECFSIknyTLYKVSEYGT 319
gi 53734636 131 GVWEYAHN-----KGIPDETCNNYQA------KDQDCKpfNQCGTCTTf------------GVCNIVknfTLWKVGDYGS 187
gi 60467666 135 DAFAFINE-----NGIVDETCKPYQAk----nLPDECS--PACKTCNPd------------GTCQAIpvhTNITVTEYGS 191
gi 50657031 134 GAYKFIFK-----NGITDITCSPFMGvdsfywAEVPCNe-TMCRTCDRf------------GKCSFIk-gPTYFISEYGT 194
gi 29150712 126 GVYEYALK-----EGIPDDTCSPYLA------HDTECLpkIRCSTCTEm------------QSCFVIkeyQKYFIKDYSY 182
gi 47028329 133 GPYQWIKSksdkgSGISYETSNPYLAcss-esEEGICG--NADWSCTPmneartcstfppqGKCLPIkqfPMASISAYGT 209
gi 17562680 298 NVLEHAKI-----QGLVEEGCNVYRA------TNGECNpyHRCGSCWP-------------NECFSLtnyTRYYVKDYGQ 353
gi 60469326 127 EAYAYMAA-----KGITDETCAPYEA------IDNECNaeGICKNCNFdls-------nptADCFAQptyTTYFVEEHGQ 188
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                      #                        #       
1EF7_A      135 LSGREKMMAEIYANGPISCGIMATe-rLANYTGGIYAEYqd-tTYINHVVSVAGWGISD-----GTEYWIVRNSWGEPWG 207
1DEU_A      170 LSGREKMMAEIYANGPISCGIMATe-rLANYTGGIYAEYqd-tTYINHVVSVAGWGISD-----GTEYWIVRNSWGEPWG 242
gi 47939805 187 VSGREKMMAEIYKNGPISCGIMATd-kLDAYTGGLYAEYqp-rAMINHIISVAGWGLDEn----GVEYWIVRNSWGEPWG 260
gi 50758927 310 VSGREKMMAEIYANGPISCGIMATe-kLDAYTGGLYTEYnp-sPTVNHIVSVAGWGVEN-----GTEYWIVRNSWGEPWG 382
gi 17507081 320 VHGYEKMKAEIYHKGPIACGIAATk-aFETYAGGIYKEVt--dEDIDHIISVHGWGVDHe---sGVEYWIGRNSWGEPWG 393
gi 53734636 188 ASGLDKMKAEIYSGGPISCGIMATd-kLDAYTGGLYSEYvq-ePYINHIVSVAGWGVDEn----GVEFWVVRNSWGEPWG 261
gi 60467666 192 VRGAKDMMAEIYARGPIACSIDATs-kLEAYTSGIFKEFkl-dPLPNHIISVIGWGVQD-----STPYWIVRNSWGSYYG 264
gi 50657031 195 VTGEDQMKAEVFARGPIACSVYAHsaaFEEYTGGVIHDPvq-yNSTTHVVAVTGWGTDEk---tGMKYWIGRNSFGTAWG 270
gi 29150712 183 LSGEDNIINEMFARGPLSCSMYASenfVFNYTGGVYVENs--nSLPNHLVSILGWGEDVdehdkVRPYWIIRNSWGTNWG 260
gi 47028329 210 ISGQTAMQKEIAARGPISCGIDAAp--ILNYTSGIADMRg---EMVDHVISVVGWGKDDt----KGSYWIVRNSWGEYWG 280
gi 17562680 354 VQGRDKIMSEIKKGGPIACAIGATkkfEYEYVKGVYSEKs--dLESNHIISLTGWGVDEn----GVEYWIARNSWGEAWG 427
gi 60469326 189 VNGSVAMMQEIFARGPIACGMEVTd-aFESYTSGVFTSSvgstGEINHEISIIGWGTEN-----GVDYWIGRNSWGTYFG 262
                       250       260       270
                ....*....|....*....|....*....|....
Feature 1                                         
1EF7_A      208 ERGWLRIVTstykdgkgaryNLAIEEHCTFGDPI 241
1DEU_A      243 ERGWLRIVTstykdgkgaryNLAIEEHCTFGDPI 276
gi 47939805 261 ERGWLRIVTsaykggkgadyNLAIEEDCAYGDPI 294
gi 50758927 383 ERGWLRIVTsaykggrgaeyNLAVEEDCAYGDVI 416
gi 17507081 394 EHGWFKIVTsqykn-agskyNLKIEEDCVWADPI 426
gi 53734636 262 EKGWLRIVTsaykggsgsqyNLAIEEDCMYGDPI 295
gi 60467666 265 EGGFFNIVQgsl------feNLGIELDCNWAVPS 292
gi 50657031 271 EDGWFKLQRgv-------naLDIEKHTCAWAVPK 297
gi 29150712 261 EKGFFRIPRssyk---dgryTLNIEKGLYFSNYR 291
gi 47028329 281 EMGYIRVAFg----------ALKVEEQCAWAEVK 304
gi 17562680 428 ELGWFRVVTskfkdgqgdqyNMGIERDCYYADVD 461
gi 60469326 263 ELGFFRIQRgi--------dLLSIESACDWAVPK 288

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