2D3J


Conserved Protein Domain Family
WIF

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pfam02019: WIF (this model, PSSM-Id:216849 is obsolete and has been replaced by 426564)
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WIF domain
The WIF domain is found in the RYK tyrosine kinase receptors and WIF, the Wnt-inhibitory- factor. The domain is extracellular and contains two conserved cysteines that may form a disulphide bridge. This domain is Wnt binding in WIF, and it has been suggested that RYK may also bind to Wnt. The WIF domain is a member of the immunoglobulin superfamily, and it comprises nine beta-strands and two alpha-helices, with two of the beta-strands (6 and 9) interrupted by four and six residues of irregular secondary structure, respectively. Considering that the activity of Wnts depends on the presence of a palmitoylated cysteine residue in their amino-terminal polypeptide segment, Wnt proteins are lipid-modified and can act as stem cell growth factors, it is likely that the WIF domain recognises and binds to Wnts that have been activated by palmitoylation and that the recognition of palmitoylated Wnts by WIF-1 is effected by its WIF domain rather than by its EGF domains. A strong binding affinity for palmitoylated cysteine residues would further explain the remarkably high affinity of human WIF-1 not only for mammalian Wnts, but also for Wnts from Xenopus and Drosophila.
Statistics
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PSSM-Id: 216849
Aligned: 11 rows
Threshold Bit Score: -1
Threshold Setting Gi: 0
Created: 22-Dec-2011
Updated: 16-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
2D3J_A        14 LWIDAHQARVLI------GFEEDILIVSEGKMAPFTHD-----FrkaqqrMPAIPVNIHSMNFTWQAaGQAEYFYEFLSl 82  human
Q6GLP3        55 LYLSEEEVRRLI------GLDAELYYIRNDIINHYALS-----F------NLLVPSETNSLHFTWHAkSKVEYKLGFQV- 116 African clawed ...
Q9W6F8        33 MWIDAHQARVLI------GFEEDILIVAEGKMAPFTHD-----FrkaqqrMPAIPVNIHAMNFTWQAtGQAEYFYEFLSl 101 African clawed ...
Q2F5U3        41 LWIDERQVRMFS------GISMQVFAIVNGHVSPYVLDpnfsnK------LPIIPSEVGYVNFTWKS--KKRYYYNFDTl 106 domestic silkworm
Q29IM0       103 LWINEQQLKMLSalyfpqGYSDRIYAIHNSLVTNDLRDtt-lyN------FLVIPSEVNYVNFTWKS-GRRKYFYDFDRl 174 Drosophila pseu...
Q52ZF0        40 LLITKAEMNRTL------GVNAELAYIENGEVNNYSTK-----Y------AYRVDSHVGELKFTWNApTNNKIHYSMQLd 102 Pristionchus pa...
Q7JQT0        29 IFISHHEVMKLM------GLEADLFYVHEGAINTYAMH-----F------TVPVPADVHELEFSWQSlIAYPLPYAISIe 91  fruit fly
Q27324        24 IFLNLHEVLRLI------GVSAELYYVREGAINDYALN-----F------AVPVPANISDVTFTWQSlVDHPLPYSINIa 86  fruit fly
Q9V422        49 VFLNPVEVMRLL------GVSAEVYYVREGHINNYALN-----F------IVPVPANVKDISFTWQSlAGRGLPYSINVv 111 fruit fly
NP_001077295  62 IFMSEEEVKKLL------GLDAELYYVRDDVVNHYALS-----F------VLPVPSDTNSLHFTWHSsSKVDYRLGFHV- 123 zebrafish
Q16Q72        45 LWINEQQVKIFS------GVPTKIYAIDNGRVSLHLRDpnfshY------LPIIPSEVSCVNFTWKS-GSKKYHYNFDRl 111 yellow fever mo...
Q6GLP3        55 LYLSEEEVRRLI------GLDAELYYIRNDIINHYALS-----F------NLLVPSETNSLHFTWHAkSKVEYKLGFQV- 116 African clawed ...
Q9W6F8        33 MWIDAHQARVLI------GFEEDILIVAEGKMAPFTHD-----FrkaqqrMPAIPVNIHAMNFTWQAtGQAEYFYEFLSl 101 African clawed ...
Q2F5U3        41 LWIDERQVRMFS------GISMQVFAIVNGHVSPYVLDpnfsnK------LPIIPSEVGYVNFTWKS--KKRYYYNFDTl 106 domestic silkworm
Q29IM0       103 LWINEQQLKMLSalyfpqGYSDRIYAIHNSLVTNDLRDtt-lyN------FLVIPSEVNYVNFTWKS-GRRKYFYDFDRl 174 Drosophila pseu...
Q52ZF0        40 LLITKAEMNRTL------GVNAELAYIENGEVNNYSTK-----Y------AYRVDSHVGELKFTWNApTNNKIHYSMQLd 102 Pristionchus pa...
Q7JQT0        29 IFISHHEVMKLM------GLEADLFYVHEGAINTYAMH-----F------TVPVPADVHELEFSWQSlIAYPLPYAISIe 91  fruit fly
Q27324        24 IFLNLHEVLRLI------GVSAELYYVREGAINDYALN-----F------AVPVPANISDVTFTWQSlVDHPLPYSINIa 86  fruit fly
Q9V422        49 VFLNPVEVMRLL------GVSAEVYYVREGHINNYALN-----F------IVPVPANVKDISFTWQSlAGRGLPYSINVv 111 fruit fly
NP_001077295  62 IFMSEEEVKKLL------GLDAELYYVRDDVVNHYALS-----F------VLPVPSDTNSLHFTWHSsSKVDYRLGFHV- 123 zebrafish
Q16Q72        45 LWINEQQVKIFS------GVPTKIYAIDNGRVSLHLRDpnfshY------LPIIPSEVSCVNFTWKS-GSKKYHYNFDRl 111 yellow fever mo...
2D3J_A        83 rsLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVDVIVMNSEG----NTILQTPQNAIF 149 human
Q6GLP3       117 --DNPMAMTMPQANISMQGEVPSSLTVFRVDLYCSGKMDSEVLFLMQLNITISSKn---ITVLNFKRRKMC 182 African clawed frog
Q9W6F8       102 rsLDKGIMADPTVNMPLLGTVPHKATVIQVGFPCLGNQDGVAAFEVNVIVMNSEg----NVILQTPQNAIF 168 African clawed frog
Q2F5U3       107 ksSDLKILKAPVLSIKTQGRVPKTAKEFSIILPCVGNSSGVATFEIGLVLKNARGlplkGTPLRLNLKKEC 177 domestic silkworm
Q29IM0       175 qtMDDSILKAPTLSIRKSGRIPQEQKNFSIFLPCTGNSSGTASFNVGLKIQTRNNkplsGTPIRLNFKKEC 245 Drosophila pseudoobscura
Q52ZF0       103 --SDPQVV--AKLRHSPSGFLPRNPSEFSIEYRCAGTRSGQFEVVLTIEFTHEDSk---TTRVRLRQEKIC 166 Pristionchus pacificus
Q7JQT0        92 ynNDQEALGTPTLSIPHKGLVPQEIESFLVYLPCTGNASLQMPVNVNMVVRAPPRf--nDTRLHFKRNKIC 160 fruit fly
Q27324        87 -tSDTEVLPRPILNISRIGDVPVEPQTWGIALKCSGTRNAEVTVTINVEVILDRAtn-nNTNLIFKRKKIC 155 fruit fly
Q9V422       112 -sSDQEVLPRPAINVSHSGEIPTTIQTWSIALKCSGLKAAEVDVTVSLEVVLNRSln-nVTHLVFRRKKIC 180 fruit fly
NP_001077295 124 --ENTSAMDQPQSNISVQGEVPRTRSVFRVDLFCSGKADGEAVLTVQLNLSTAAn----FTVLNFKRRKMC 188 zebrafish
Q16Q72       112 vsEDEQILKPPTISIKTSGRVPKNAKEFSVTLPCSGNVSGIAVFSIGLLIQSRKGkpvpGTPLRIKLRKEC 182 yellow fever mosquito
Q6GLP3       117 --DNPMAMTMPQANISMQGEVPSSLTVFRVDLYCSGKMDSEVLFLMQLNITISSKn---ITVLNFKRRKMC 182 African clawed frog
Q9W6F8       102 rsLDKGIMADPTVNMPLLGTVPHKATVIQVGFPCLGNQDGVAAFEVNVIVMNSEg----NVILQTPQNAIF 168 African clawed frog
Q2F5U3       107 ksSDLKILKAPVLSIKTQGRVPKTAKEFSIILPCVGNSSGVATFEIGLVLKNARGlplkGTPLRLNLKKEC 177 domestic silkworm
Q29IM0       175 qtMDDSILKAPTLSIRKSGRIPQEQKNFSIFLPCTGNSSGTASFNVGLKIQTRNNkplsGTPIRLNFKKEC 245 Drosophila pseudoobscura
Q52ZF0       103 --SDPQVV--AKLRHSPSGFLPRNPSEFSIEYRCAGTRSGQFEVVLTIEFTHEDSk---TTRVRLRQEKIC 166 Pristionchus pacificus
Q7JQT0        92 ynNDQEALGTPTLSIPHKGLVPQEIESFLVYLPCTGNASLQMPVNVNMVVRAPPRf--nDTRLHFKRNKIC 160 fruit fly
Q27324        87 -tSDTEVLPRPILNISRIGDVPVEPQTWGIALKCSGTRNAEVTVTINVEVILDRAtn-nNTNLIFKRKKIC 155 fruit fly
Q9V422       112 -sSDQEVLPRPAINVSHSGEIPTTIQTWSIALKCSGLKAAEVDVTVSLEVVLNRSln-nVTHLVFRRKKIC 180 fruit fly
NP_001077295 124 --ENTSAMDQPQSNISVQGEVPRTRSVFRVDLFCSGKADGEAVLTVQLNLSTAAn----FTVLNFKRRKMC 188 zebrafish
Q16Q72       112 vsEDEQILKPPTISIKTSGRVPKNAKEFSVTLPCSGNVSGIAVFSIGLLIQSRKGkpvpGTPLRIKLRKEC 182 yellow fever mosquito
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