Conserved Protein Domain Family
LC-FACS_euk

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cd05927: LC-FACS_euk 
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS)
The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Statistics
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PSSM-Id: 213293
View PSSM: cd05927
Aligned: 37 rows
Threshold Bit Score: 672.335
Threshold Setting Gi: 729924
Created: 2-Nov-2011
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 60302804  117 QPYEWISYKEVSDRAEYVGSALLHRGfkpshSQYVGIFAq-NRPEWVIIEQSCytYSMVVVPLYDTLGtEAITYIVNKAD 195
gi 1346423    95 TPYITMTYEEMICVMHDIGRGLIKIGvkpngENKFHIFAs-TSHKWMKTFLGCmsQGIPVVTAYDTLGeSGLIHSMVETD 173
gi 75206264  125 GEYKWQSYGEVFERVCNFASGLVNVGhn--vDDRVAIFSd-TRAEWFIAFQGCfrQSITVVTIYASLGeEALIYSLNETR 201
gi 162662902 105 GQYEWTTYAQAFLRASDFASGLVALGhg--qGERVAIFSe-TRPEWLLALQACfrRNLTVVTIYASLGeDALVHSLSETE 181
gi 17553312  137 GEYVWQSYKEVETQVARLAAGIKDLAhe-eqNPKVVIFAe-TRADWLITALACfrANVTIVTVYATLGeDAIAHAIGETE 214
gi 74582344   92 SDYNYLSFNDIYDKALRYAGALRKLGln--kGDKFELYAp-TSAFWLLTAEAClsQSMTIVTAYDTLGeEGLLHSLRESG 168
gi 116505024 104 SEYQYINYIELQEIVSEIARGLHHLGit--eNDIFNIYAq-TSPTWQYMSHGCgsISIPIATAYDTLGeEGLIHSLNEPE 180
gi 74638740   96 GPYEYISFNKVYEIALALGSGLVASGi---tSETTMLFFaaTSAKWFTTAQGCssQAIPIVTAYETLGeDGIYTSLDECK 172
gi 83774987  108 SGYSYKSFVEYEQMALQLGAGLKKLGle--tGDKIHLYGa-TSENWLAMSHGAasQSLTIVTAYDTLGeEGLKHSLVQTS 184
gi 254351247  89 GPYNYLSYSQYELLAIQIGSGLRKLGls--sSNKVYLFGt-TSANWISMSHGCasQGIPIVTGYDTLSaTDIQHSLSQTH 165
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 60302804  196 LSLVFCDTpekakllltavekgetpils-------------tivimepfgtdllergkkcgVEVFSMKEIEELGKahrq- 261
gi 1346423   174 SVAIFTDNqllsklavplktaknvkfvihnepidpsdkrqngklykaakdavdkikevrpdIKIYSFDEIIEIGKkakde 253
gi 75206264  202 VSTLICDSkqlkklsaiqsslktvknii--------------yieedgvdvassdvnsmgdITVSSISEVEKLGQknav- 266
gi 162662902 182 VSTVICDSkqlkkivdlsdklgtvkr-----------------viymedddtvtsepsmegKVIARFSHVEKLGKqspa- 243
gi 17553312  215 ATILVTSSellpkivtlgkkcptlktliy------------fapvdqkapapelapfrdqfKHVLSLSGLLTRNQeqv-- 280
gi 74582344  169 VRGMYTEGhllktlvnplkeieslevii--------------yrndakeediktiqeirpnLKLIKFADFEKMSPpve-- 232
gi 116505024 181 CIGVFTNAellptlnrvlaktptvkyii--------------ydgepssalldnlrsvrdtIQVYSLTQLRELGRdkpve 246
gi 74638740  173 SRAIFTDPnlipkllgplkqstwvkliv--------------csstpsedlvelvkstapdVEIITYDNLLSLGKekpq- 237
gi 83774987  185 SVAMFCDPslipsvanvlkdvksikhiiw------------nshfapkqadlerlkteyedINVISFEDLRTLGEqnpv- 251
gi 254351247 166 AEVIYLDPhllgtasialensqvktviin------------tgsifsggydidqfrnehpqFNVITYEELIQLGRhnlk- 232
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                         #                                           ##                 
gi 60302804  262 --kpiLPKPEDLAVICFTSGTTGNPKGAMITHKNIVSNSSAFLKTtektfiptpEDVLISFLPLAHMFERIVECVVLCHG 339
gi 1346423   254 -velhFPKPEDPACIMYTSGSTGTPKGVVLTHYNIVAGIGGVGHNvigw--igpTDRIIAFLPLAHIFELTFEFEAFYWN 330
gi 75206264  267 --qpiLPSKNGVAVIMFTSGSTGLPKGVMITHGNLVATAAGVMKVvpk---ldkNDTYIAYLPLAHVFELEAEIVVFTSG 341
gi 162662902 244 --qpdMPRPSDIAVIMYTSGSTGMPKGVMMSHKNIVATIAGVTASvpk---lntSDVYLAYLPLAHILELAGESTLFAAG 318
gi 17553312  281 --kesTAVKSDIALIMYTSGTTGQPKGVILLHQNVVAALLGQGDGvgi---icnADTYIGYLPLAHILELDAELTCLTKG 355
gi 74582344  233 ---pdPPSPEEICCIMYTSGSTGLPKGVILSHKNMVAIVTAIVKHvpe---vtsKDYLLAYLPLAHILEFAFENICLAWG 306
gi 116505024 247 plearRPKPETIACIMYTSGSTGNPKGVVLKHSNLIAGVGAFKVLighh--lgrEDSILAYLPLAHVLEYMVELCSLFAG 324
gi 74638740  238 --pphPPKADDICCYMYTSGSTGKPKGVVLLHRNIIAALGGINRIlsqh--invKDYVLAYLPLAHIFEFIFEMCCLYWG 313
gi 83774987  252 --epvPPSPEDLCCIMYTSGSTGPPKGVPLTHANVIAATAGINTIvgpy--igpSDALLTYLPQSHILEFMFENLCLIWG 327
gi 254351247 233 --epiPVKSSDLFCIMYTSGSTGLPNGCCITHENFLAGITGLLGGiddf--vsdQERVLAYLPLAHIFEMALENLVMYIG 308
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                            # ##  #                      ##             
gi 60302804  340 ARIGFFqgdi---------rllMDDLKTLQPTVFPVVPRLLNRMfdki--fgqADTSLKKWMLDFASKRKeaelrsg--- 405
gi 1346423   331 GILGYAnvktltp---tstrncQGDLMEFKPTVMVGVAAVWETVrkgilakinELPGWSQTLFWTVYALKernip----- 402
gi 75206264  342 SAIGYGsamtltdtsnkvkkgtKGDVSALKPTIMTAVPAILDRVregvlkkveEKGGMAKTLFDFAYKRRlaavdgswfg 421
gi 162662902 319 AAIGYGspltltdtsskikfgtKGDATELQPTLMSAVPAILDRVrdgvrkkvnTKGGITKKLFELAYYRRlaaiegswfg 398
gi 17553312  356 AKVGYSspltlhdraskiqkgtHGDCHALRPTLMAAVPAIMDRIfkavseevaASPRFMQELFKLNYERKrsryle---- 431
gi 74582344  307 GTIGYAnvrtlvd---tncrncKGDINTFRPTIMVGVPAVWEMVrkgimsklnAASAVKRSVFWTAYYTKaklmrhn--- 380
gi 116505024 325 MTIGYGrvktltd---asvrhcKGDISEFKPTRFVGVPAVWENIrkgilakvdTAGGLKKVIFNGAYWAKsnkip----- 396
gi 74638740  314 GVLGYAsprtltd---asvvncKGDLTEFRPTVLIGVPAVYELIkkgilakvsSMPAHRQKVFSGSLSLKqyliern--- 387
gi 83774987  328 GTMGYGnprtlsd---asmrncKGDIREFKPTILVGVPAVWESVkkgvlnnlnKNNLIVRGMFWGAMAAKnflmttgfp- 403
gi 254351247 309 GTLGYGnpktltd---aslrecNGDMVEFKPTIMVGVPQIWETIrkavlsklnCSGFVAKTVFWTAMSFKsfavrys--- 382
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                            ######                      
gi 60302804  406 -iirnnsfWDKVIFRKIQASLGGKVRLMVTGAAPVSASVLTFLRtalGCQFYEGYGQTECtAGCSLSMPGDWt--AGHVG 482
gi 1346423   403 ----csglLSGLIFKRIREATGGNLRFILNGGSAISIDAQKFLSn-lLCPMLIGYGLTEGvANACVLEPEHFd--YGIAG 475
gi 75206264  422 awglekmlWDALVFKKIRAVLGGHIRFMLVGGAPLSPDSQRFINicmGSPIGQGYGLTETcAGATFSEWDDPa--VGRVG 499
gi 162662902 399 awgleamlWNVLVFRKIRSALGGRVRGMLSGGAPLSGDTQRFMNicfGCPIGQGYGLTETcAGGTFSNWEDMt--VGRVG 476
gi 17553312  432 --gycspfLDRIVFKKIRRLLGGKLRGVLSGGAPLNAETQRFMNicmCCPVVQGYGLTETcGAACVADINDLs--TGTVG 507
gi 74582344  381 -lpgscvlDTAVFNKIRSMGTGGRLRYTLSGGSALSPDTKRFLSi-vLCPMLIGYGLTEIsAAAMVQNPACFn-lDDSAG 457
gi 116505024 397 ---ilstiADKVVFGGVRAATGGRIAFALSGGAAISPETHEFLSt-aLVDISQGYGMTETcGMISLVPPELMr--YKSVG 470
gi 74638740  388 --lpgsaaLDAIVFNKVKAATGGRLRYCISGGAALAASTQAFLSs-cICPVLPGYGLTETcAGSFVLSPEQWhlyANTVG 464
gi 83774987  404 ghsvgsslLDAVVFKKLKDATGGRLRIMMNGGGPISKDTQKFLSm-aIAPMISGYGLTETsAMGALNDPMAWn--PDALG 480
gi 254351247 383 --lpgkgvFDDLVFGRVRQMTGGRLRYILNGSSGIADSTKEFLSl-iVAEMLTGYGLTETcANGALSSPFEQt--TSAIG 457
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                          #           # 
gi 60302804  483 APMACNIIKLVDVQEMNYLAak----gEGEVCVKGPNVFLGYLKDPEKTAEal----dkDGWLHTGDIGKWLPNGTLKII 554
gi 1346423   476 DLVGTITAKLVDVEDLGYFAkn----nQGELLFKGAPICSEYYKNPEETAAaf----tdDGWFRTGDIAEWTPKGQVKII 547
gi 75206264  500 PPLPCGYVKLVSWEEGGYRIsdk-pmpRGEIVVGGNSVTAGYFNNQEKTDEvykvdekgTRWFYTGDIGRFHPDGCLEVI 578
gi 162662902 477 PPVAVCYLKLVNWDEGGYKYtda-pmpRGEIVIGGPNVTMGYFKNQAKTDEvykvdekgMRWFYTGDIGRFHEDGCIEII 555
gi 17553312  508 PPVRCSDIALREWSEGGYSPfnd--ppQGEILISGENISPGYYKQPEKTAEdfit-ykgKVYFATGDIGQKRDDGSLLII 584
gi 74582344  458 SLLPCTEMKLVDCEEGNYNShgh--ppRGEIWLRGPSLTRGYLNRDKENKEsf----tpDGWFRTGDVGELTPEGLLRII 531
gi 116505024 471 IPFPSVEIKLLDVPDAGYFStnd--ppQGEVCVRGPSVTSGYYKRPDLNNDesi---fkGGWLRTGDVGQWNPDGTLTLI 545
gi 74638740  465 FPIPSIEFKLVDIPDLGYYTdss--ppRGEVWIRGPAVCNGYLNRPEDNKAaf----teDGWFKTGDVGEIAKGNTLRLI 538
gi 83774987  481 EIPASIEVKLVDFADAGYFTksn--ppQGEIFIRGGSVSKGYWDNEEETKAay----teDGWFMTGDIGEFDKNGHLKII 554
gi 254351247 458 STSPAIDVKLVSIPELGYFTdadagpcQGEILVRGPAVFKGYFNNPQGTEKaf----apGGWFKTGDIGEFDDRGHLKII 533
                        490       500       510       520       530       540       550       560
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         #        ####                                                                  
gi 60302804  555 DRKKHIFKLAQGEYIAPEKIENVYLRSEALAQVFVHGESLQAFLLAVVVPDPETLCKWakk-------kgfEGTYEKLCK 627
gi 1346423   548 DRKKNLVKTLNGEYIALEKLESIYRSNPYVQNICVYADENKVKPVGIVVPNLGHLSKLaielg----imvpGEDVESYIH 623
gi 75206264  579 DRKKDIVKLQHGEYVSLGKVEAALGSSNYVDNIMVHADPINSYCVALVVPSRGALEKWaeea------gvkHSEFAELCE 652
gi 162662902 556 DRKKDIVKLQHGEYISLGKVEAVLSASLYVENIMLHADPFHSYCVALVVVSQSALEGWaqda------nvdYSDFADLCS 629
gi 17553312  585 DRKKDLVKLQHGEYVSLAKVECALLNCPIVDNICVYGSGMEDSVVALVVPNQKHLEKIaeae------gvsTSEMKTMCE 658
gi 74582344  532 DRKKNLVKTQNGEYIALEKLESRYRTSSLVSNICVYADQTKVKPLAIIVPNEPVVRKLateqa----glspDASWEEVCH 607
gi 116505024 546 DRIKNLIKLAQGEYIALERLESIYKSCNLVANICVHAVPAANQPMGIIIPQEVHLRNTlasl-----nldsTSDLSVLCA 620
gi 74638740  539 DRKKNIVKSLNGEYIALEKIEAQFFTSPLVSNVCAYADVNHAKPVVIVNPDENGLRTYltknsgssfngnpNDTLTNLCK 618
gi 83774987  555 DRKKNLVKTQNGEYIALEKLESVYRSSPLVGNICVYAAQDQDKPIAIIVPVEAALKKLahen------gieGDTLETLVH 628
gi 254351247 534 DRIKSLVKMQGGEYIALEKLESIYRTSQAILQVMVHADFEYTRPIVIIMPNTKFLQDKsre--------lgFSDDDSTLS 605
                        570       580       590       600       610       620
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
Feature 1                               #                                           
gi 60302804  628 NKDVKKHIleDMVRVGKESGLKSFEQVKDIIVHTEmFSIENGLLTPTLKAKRPELRKYFQSQIDELY 694
gi 1346423   624 EKKLQDAVckDMLSTAKSQGLNGIELLCGIVFFEEeWTPENGLVTSAQKLKRRDILAAVKPDVERVY 690
gi 75206264  653 KGEAVKEVqqSLTKAGKAAKLEKFELPAKIKLLSEpWTPESGLVTAALKIKREQIKSKFKDELSKLY 719
gi 162662902 630 KTQAIKEVlsSLTKIGKENRLEKFEIPMKIKLMSElWTPESGLVTAALKLKRENIRKTFAEDLKSLY 696
gi 17553312  659 DKKVIAAYkkQLEEHAKKSKLSRSEIPAAIHLCPEiWTPDTGLLTEALKLKRKPIQMAYQKTLDDLY 725
gi 74582344  608 NKKVRQLVydDLIRIGRSHHFANIELIQNVVLVPIeFTPENGLVTAAQKLQRRKILDRFKKEIDAAY 674
gi 116505024 621 KDEVRKLIlkECNAVGRRNGFKPVELLQDVVLTAEeWTPENGLVTAAQKIQRVKIAKTFHEQIEAVY 687
gi 74638740  619 DSGVQHLIlkELINIGKQQRLASIEIPEGVVLSDFeWTAENNFLTASRKVKRQVIVAHYSDEIQKAY 685
gi 83774987  629 NEKLKGIVlkQLQTAGRAGGLKGIEIINGVVLSDEeWSPQNGYMTAAQKLQRKKIVGHFQKEIDQAY 695
gi 254351247 606 SERMSAYVldDLQDIARRSGLSKIETVTGVVITDIeWTPQSGLVTPTMKLNRRFILNYFRDEVEKCM 672

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