2WYI,2WYH,3LVT,2WYH


Conserved Protein Domain Family
GH38N_AMII_SpGH38_like
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cd10814: GH38N_AMII_SpGH38_like 
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N-terminal catalytic domain of SPGH38, a putative alpha-mannosidase of Streptococcus pyogenes, and its prokaryotic homologs; glycoside hydrolase family 38 (GH38)
The subfamily is represented by SpGH38 of Streptococcus pyogenes, which has been assigned as a putative alpha-mannosidase, and is encoded by ORF spy1604. SpGH38 appears to exist as an elongated dimer and display alpha-1,3 mannosidase activity. It is active on disaccharides and some aryl glycosides. SpGH38 can also effectively deglycosylate human N-glycans in vitro. A divalent metal ion, such as a zinc ion, is required for its activity. SpGH38 is inhibited by swainsonine. The absence of any secretion signal peptide suggests that SpGH38 may be intracellular.
Links
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Statistics
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PSSM-Id: 212125
Aligned: 26 rows
Threshold Bit Score: 366.584
Created: 31-Mar-2011
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:2WYI; Streptococcus pyogenes alpha-mannosidase (SpGH38) binds swainsonine/Zn2+ complex, contacts at 4A.
  • Comment:A divalent metal ion, such as zinc ion, is required for the catalytic activity.
  • Comment:SpGH38 can be considered a five-domain protein, this is one of four domains which contributes residues to the active site
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                # #  #                                                                  
2WYI_A        27 KVHIISHSHWDREWYMAYEQHHMRLINLIDDLLEVFQTDpdfHSFHLDGQtIILDDYLKVR--PEREPEIRQAIASGKLR 104  Streptococcus ...
ZP_06242930    2 KFYYFNSTHWDREWYQPFQEFRKYLVDTARVLLEIFERDpdyRRFTFDGQtIVLEDICEIR--PDWRPKLEALIRAGKLN 79   Victivallis va...
YP_002940205   7 RVHVLSHTHWDREWYAAFETFRKRLLNLIDSLIENLPENphfKHFMLDGQtIVLEDYLELR--HQKKDELVEFIREGKIS 84   Thermotogales ...
ZP_06244401    3 QFHVISHTHWDREWYQPFEVFRHRLVDLIDHLLEIYREYp-eYVFELDAQtVCLEDYLEVR--PYRRAELERYIRSGNLK 79   Victivallis va...
ZP_06113705    3 KILIAASTHWDREWYRTFQEFQIRLCGLMNRLITLLEQDeefLCYTFDGQsAVLEDYLEIY--PENRGRIEKLAAEGRLV 80   Clostridium ha...
YP_004694740   3 TIHVICHTHWDREWYLTFQQFRLRLVHLVDGLLDILAEDhnyKHFMLDGQtIMLGDYLIMR--PEAEDTIKDHVRNGRIL 80   Nitrosomonas s...
YP_004528823   7 TIYAVSKTHWDREWYLDFQNFRVRLVRLVDNLLDILERDpgyVSFMMDGQmIPIEDYLAVK--PEAFDRIKKLVLDGRLV 84   Treponema azot...
ZP_01168672    6 EVHILNHTHWDREWYETFEEFRYKLRNGLRYIQTLLEEGk-iENFFLDGQtIVLDDYREVVg-EEEYQKLLSYINDGKIE 83   Bacillus sp. N...
ZP_06178082    5 ICSVVFQTHWDREWYLPFETFRARLIRVMERVVDALEKHe-lESFLFDGQvVAAEDLLEACe-PELAEKLHRLMQEGRLV 82   Vibrio harveyi...
YP_004070213  21 VIYFLPHTHWDREWYLPFQIFRARLLHVIDVLLEMLEKFp-eFKFNLDGQvIALEDYLEIEkdPQKTETLFSMIRNGRIS 99   Thermococcus b...

Feature 1                                                     # #                     #          
2WYI_A       105 IGPFYILQDDFLtSSESNVRNMLIGKEDCDRWGa---SVPLGYFPDTFGNMGQTPQLMLKAGLQAAAFGRGIrptgfnnq 181  Streptococcus ...
ZP_06242930   80 VGPWYVMPDEFLvSGEALIRNLLTGKKVAEEFGh--aPWPVGYVCDIFGHIAQLPQILAGFRLKGSVAWRGTedk----- 152  Victivallis va...
YP_002940205  85 VGPWYILPDEFLiTGEALIRNFLIGKSVLDKLGiggnVMKIGYLPDMFGHSAYTPVILKGLGFEGAVLWRGIgdk----- 159  Thermotogales ...
ZP_06244401   80 VGPWYVQNDFFLtGGEATVRNLLAGTALAEEFGa---CARCGYTPDQFGLIGQLPQVFRGFGIDNCVFGRGYrreg---- 152  Victivallis va...
ZP_06113705   81 FGPLYNLPDEFLsGGEALIRNFLLGDEVCQSVGg---KLNAGYVPDNFGHISQLPQILNGVGIDTAFFFRGMdrt----- 152  Clostridium ha...
YP_004694740  81 IGPWHILPDMFLvSPEAHIRNLLAGDREARKFGq---KMMIGYMPDSFGHIGQMPQILRGFGIDNACLWRGLdd------ 151  Nitrosomonas s...
YP_004528823  85 IGPWYVLPDEVLiSGESHIRNWLMGERLSRKFGk---KMNIGYLPDSFGHPSQMPQILAGLGMKQMTFWRGAted----- 156  Treponema azot...
ZP_01168672   84 AGPWYLLADEFLvSGESMIKNLEIGTKMAKAAGs---SCDIGYLPDTFGHISQMPQILKQSGISTALIFRGAvs------ 154  Bacillus sp. N...
ZP_06178082   83 LGPWYVMADEFLcSGESLIRNLEIGRKLANSLGn---YQKVGYLPDTFGHIGQMPQLLTGFDINNIVLWRGIda------ 153  Vibrio harveyi...
YP_004070213 100 VGPFYVLPDEFLiSGETFIRNFLIAQRVLEELSl--pSMKVAYFPDMFGHSAYVPTLIRGLGMDSVLIWRGVgdk----- 172  Thermococcus b...

Feature 1                                          #                                             
2WYI_A       182 vdtsekyssqFSEISWQGPDNSRILGLLFA--NWYSNgn--------eIPTTEAEARLFWDKKLADAErfa---stKHLL 248  Streptococcus ...
ZP_06242930  153 --------grEHLLWWEAPDGTRLPHINLGkwCGYSEftfdvrgnhsaEPLDEQAFKTRLREYVERDRnr----wnGSFI 220  Victivallis va...
YP_002940205 160 --------srKTEFLWKAPNGDEILVINLI--RSYSNgahf----grdIELMKSVFKKEIEELSKHATt-------ENIL 218  Thermotogales ...
ZP_06244401  153 -------eaqPNEFEWCSPDGSAVLASFMS--RWYNNaq--------rFPEDPELALLFFREVEAAQEsea---vtPHRL 212  Victivallis va...
ZP_06113705  153 -------eykEKEFYWESPDGSRVLCEYMI--LGYWSlk--------sWGKMGKSTAEHFYDAYRLLKess---clNTFL 212  Clostridium ha...
YP_004694740 152 ---------qPAEFWWQSPDGSRVLLMYLR--DSYSNga--------gLNADNHAQFTEQIKKAADSLvph--sisSHFL 210  Nitrosomonas s...
YP_004528823 157 --------vdKNEFYWESPDGSKIFVNLMP--IGYSTga--------eFPDDPDLLAKRLDKYIDDSSpra---stDVLY 215  Treponema azot...
ZP_01168672  155 ---------dHFENTWEGPDSSKVFTFVLPlfEGYYQ-----------TFLKHDAYVEETKTYLEGSSpyl---tfGKAL 211  Bacillus sp. N...
ZP_06178082  154 ---------nQSELRWKGADGSEIFTLFLT--EGYYQ-----------HPLNTDDFATNIDVYLNKITera---ttDNLL 208  Vibrio harveyi...
YP_004070213 173 --------crDTEFIWKGPDGSEVLCINII--RGYWN-----------LGVPSSDYHEFKRMVEKEVEflfkhspsGHIL 231  Thermococcus b...

Feature 1             #                                                           
2WYI_A       249 MMNGCDHq-pVQLDVTKAIALANQLypDYEFVHScFEDYLADLADDLpe---nlSTVQGEITSQE 309  Streptococcus pyogenes M1 GAS
ZP_06242930  221 LSDGFDHa-cPSPDTSKMLRWIQEAypDAGVIHTdYTELFDHEFGCAe-----rPVIRGEQIQPA 279  Victivallis vadensis ATCC BAA...
YP_002940205 219 IMNGTDHe-fPLFNLPERFQEWSEEf-GAKIIHSsLQEYLKSVKSQKpe----lKTVEGELKDPK 277  Thermotogales bacterium TBF 1...
ZP_06244401  213 LMNGVDHl-eAQENLLPVLEELNRRiaPDVIRQStLCGYLEAVRTFAaehgialHRETGELRYGA 276  Victivallis vadensis ATCC BAA...
ZP_06113705  213 LINGSDHl-yQDPDFTAMVREVKEAypELDIKNGsIADYAEMAKAAAsqs-dclRTVEGEMRDFR 275  Clostridium hathewayi DSM 13479
YP_004694740 211 VMYGTDHm-aPPNGITKAIQAANKNlnEYCVIHStLPAYLQAVQAEItereseiSIVVGELRSSK 274  Nitrosomonas sp. Is79A3
YP_004528823 216 LSNGGDHl-eAVPYLSKVIKGANQKmkKGKIVHTtLEEFFTALEKEIkgk--siKTHKGEMMGTH 277  Treponema azotonutricium ZAS-9
ZP_01168672  212 VMNGADHt-fTATDLQERIKGLEEQnpGIKFKQSlMSEFIESYKGEEp-----qGKILGEQRDPS 270  Bacillus sp. NRRL B-14911
ZP_06178082  209 LTQGGDHlrpANGNMAERIAEFNHSrnDIELKQSdLATYLTTLQSEVsn---dlPVVKGELRGND 270  Vibrio harveyi 1DA3
YP_004070213 232 LMAGNDHr-fPFPLLPDFVRKLNSE--GLKCKIVtLDEYAQILQRMEls----lKKIEGEMRDSK 289  Thermococcus barophilus MP

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