1OIW,3BFK,3CPJ


Conserved Protein Domain Family
Rab11_like

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cd01868: Rab11_like 
Click on image for an interactive view with Cn3D
Rab GTPase family 11 (Rab11)-like includes Rab11a, Rab11b, and Rab25
Rab11a, Rab11b, and Rab25 are closely related, evolutionary conserved Rab proteins that are differentially expressed. Rab11a is ubiquitously synthesized, Rab11b is enriched in brain and heart and Rab25 is only found in epithelia. Rab11/25 proteins seem to regulate recycling pathways from endosomes to the plasma membrane and to the trans-Golgi network. Furthermore, Rab11a is thought to function in the histamine-induced fusion of tubulovesicles containing H+, K+ ATPase with the plasma membrane in gastric parietal cells and in insulin-stimulated insertion of GLUT4 in the plasma membrane of cardiomyocytes. Overexpression of Rab25 has recently been observed in ovarian cancer and breast cancer, and has been correlated with worsened outcomes in both diseases. In addition, Rab25 overexpression has also been observed in prostate cancer, transitional cell carcinoma of the bladder, and invasive breast tumor cells. GTPase activating proteins (GAPs) interact with GTP-bound Rab and accelerate the hydrolysis of GTP to GDP. Guanine nucleotide exchange factors (GEFs) interact with GDP-bound Rabs to promote the formation of the GTP-bound state. Rabs are further regulated by guanine nucleotide dissociation inhibitors (GDIs), which facilitate Rab recycling by masking C-terminal lipid binding and promoting cytosolic localization. Most Rab GTPases contain a lipid modification site at the C-terminus, with sequence motifs CC, CXC, or CCX. Lipid binding is essential for membrane attachment, a key feature of most Rab proteins. Due to the presence of truncated sequences in this CD, the lipid modification site is not available for annotation.
Statistics
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PSSM-Id: 206660
View PSSM: cd01868
Aligned: 49 rows
Threshold Bit Score: 283.682
Threshold Setting Gi: 74834247
Created: 6-Jan-2012
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 17 residues -Click on image for an interactive view with Cn3D
Feature 1:GTP/Mg2+ binding site [chemical binding site]
Evidence:
  • Structure:1OIW: Human Rab11a binds GTP-gamma-S, a GTP analog and Mg2+, defined using 3.5 A contacts
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                  #######          #  #  #                             #               
1OIW_A       27 DYLFKVVLIGDSGVGKSNLLSRFTRNEFNlESKSTIGVEFATRSIQVD----GKTIKAQIWDTAGLERYRAITSAYYRGA 102
gi 28628539   6 DHLYKLILIGDSAVGKSNILSRFTSNKFNaSSAATIGVEFTTKTLTVEvqgkMISTRLQCWDTAGQERYRSITSSYYRGA 85
gi 74834400   9 DLLYKLVLIGDSGVGKTNILNQFTRGEFLqDSKTTIGVEFASKNIMID----DKTVKAQIWDTAGQERYRAITSAYYRGA 84
gi 74834407   9 DYLFKVVLSGDSGVGKSNLLLRFTKNQFNpDSKTTIGVEFATRSISIS----GKIIKAQIWDTAGQERYRAITSAYYRGA 84
gi 74831454  10 KHLFKIVLIGDSSVGKSNILSRFTKRQFNfDSQPTIGVEFATRSLTEN----GKIIQAQIWDTCGQERYKSITSAYYRGA 85
gi 74833680   8 DYQYKIVIIGDSGVGKTNIMTQFTRGEFSeETKTTVGVEFANKQLVID----DKIIKAQLWDTAGQERYRAIISSYYKGA 83
gi 74833730  10 DYLFKIVLIGDSGVGKTNILKRFINNEFQlESKPTIGVEFATKTFQQQ----GKSVKCQIWDTAGQERFRAITNAYYRGA 85
gi 74831376   8 DILMKIIIVGDSGVGKTNLVNRFAQQKFLdDSKPTVGVEFFFRHIDIM----GKTIKAQVWDTAGQEKFRAITYGYYRGA 83
gi 74834495   7 SYLFKIILIGDSGVGKTNLFNRLQNKEFQyDTRPTIGVEFINRTVRQD----GNLVKCQIWDTAGQEKFRAITNAYYRGA 82
gi 74834211  10 DFLFKIILIGDSGVGKTNILQRFVKNEFIlDSKPTIGVEFSTKTINVE----NKSVKCQIWDTAGQERYRAITNAYYRGA 85
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                ## #                          ###      
1OIW_A      103 VGALLVYDIAKHLTYENVERWLKELRDHADs--nIVIMLVGNKSDLRHLRAVPTDEARAFAEKNgLSFIETSALdSTNVE 180
gi 28628539  86 HGCLLVFDVTKRTSFEHCTEWLTELRNASAnstaCKVILVGNKVDLRHLREVTTEEATAFAQQQgLSYIETSASsGHGID 165
gi 74834400  85 VGAMIIYDITKSLTFENVDKWMKELKENADp--sLQIMIVGNKTDQKHIRQVATDCAIAYAQRNgVAFIETSAKdGTNVE 162
gi 74834407  85 IGAVLVYDITNKQSFESVERWIQEVRENADk--dIVIMIIGNKSDLKHLRAIRTESGQDLAQMYkVAFMEASAQdGTNVD 162
gi 74831454  86 VGAMLIYDITKQKTFENIEKWIQELKEYAEt--nVVAMLIGNKADLKAQRKVPSDKAAQFAENHtMAFMEVSAFdGTNVD 163
gi 74833680  84 SGALIVFDITKQSTFDNVDRWMKVQESTSNe---ISIILVGNKSDLRHLRQVSSDVSSAYASKHkIAFLETSAKdGANVN 160
gi 74833730  86 VGAFICYDITREITFKNTEKWLSELKEHADg--nIVIIMIGNKIDAVDSRAVRTDEAQDYCESQkIGFIETSALdGTNID 163
gi 74831376  84 LGAMICYDITKEQSFLNVERWIEELREHGDs--nLVMMLIGTKSDLESKRVVRNEDGTQKALQHnMAFLETSALkAANVG 161
gi 74834495  83 KGVFVCYDVTKQGTYESTLRWMSEIKQFGDh--dIVIMLVGNKIDLAEQRIVRTDEVSQFCEQNkVGYIETSALnNINVE 160
gi 74834211  86 VGAFVCYDITREITFTNIEKWLSEIKEYAPk--nIVIMMIGNKIDQANDRMVRSVEAAKLCQQNkIGYIETSAQnGLNVE 163
                       170
                ....*....|.
Feature 1                  
1OIW_A      181 AAFQTILTEIY 191
gi 28628539 166 DAFKQLVTELA 176
gi 74834400 163 DAFKNILQQIY 173
gi 74834407 163 QAFTQIIQQIY 173
gi 74831454 164 LAFSRLINEIY 174
gi 74833680 161 EAFNKLINGKA 171
gi 74833730 164 VAFNKIVANIF 174
gi 74831376 162 KAFSMLLESKS 172
gi 74834495 161 MAFNQMVTEIY 171
gi 74834211 164 EAFSNLVSEIF 174

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