1M2O,1F6B,2GAO


Conserved Protein Domain Family
Sar1

?
cd00879: Sar1 
Click on image for an interactive view with Cn3D
Sar1 is an essential component of COPII vesicle coats
Sar1 is an essential component of COPII vesicle coats involved in export of cargo from the ER. The GTPase activity of Sar1 functions as a molecular switch to control protein-protein and protein-lipid interactions that direct vesicle budding from the ER. Activation of the GDP to the GTP-bound form of Sar1 involves the membrane-associated guanine nucleotide exchange factor (GEF) Sec12. Sar1 is unlike all Ras superfamily GTPases that use either myristoyl or prenyl groups to direct membrane association and function, in that Sar1 lacks such modification. Instead, Sar1 contains a unique nine-amino-acid N-terminal extension. This extension contains an evolutionarily conserved cluster of bulky hydrophobic amino acids, referred to as the Sar1-N-terminal activation recruitment (STAR) motif. The STAR motif mediates the recruitment of Sar1 to ER membranes and facilitates its interaction with mammalian Sec12 GEF leading to activation.
Statistics
?
PSSM-Id: 206645
Aligned: 22 rows
Threshold Bit Score: 257.592
Threshold Setting Gi: 19173546
Created: 4-Feb-2003
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:GTP/Mg2+ binding site [chemical binding site]
Evidence:
  • Structure:1M2O: Saccharomyces cerevisiae Sar1 binds GppNHp, a GTP analog and Mg2+, defined using 3.5 A contacts
    View structure with Cn3D

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                #######               #                     #        
1M2O_B      5 DIFGWFRDvlaslglwnKHGKLLFLGLDNAGKTTLLHMLKNDRLATLQPTWHPTSEELAIGNIKFTTFDLGGHIQARRLW 84  baker's yeast
Q8MQT8      2 GFGDWFKSalsflglykKKATIVFVGLDNAGKSTLLAMLKNSATTTVAPTQQPTSQELVMGSIRFKTFDLGGHEVARQLW 81  Giardia intestinalis
NP_597349  19 FYEKVFQNfvks--lfgKPSSILFLGIDNAGKTTLVNKLKSDSTDVYMPTHHPSTSYIEIGNLKAQVIDLGGHTAARLAW 96  Encephalitozoon cu...
AAT09092    3 YFSGFFTGilklfgfysKEATVVLIGLDNAGKTTLQYKLRTGESYSFVPTQKPQEQEITIGNVKLQTWDLGGHKAVRKLW 82  Bigelowiella natans
CAI44555    2 FILNMFKSllgslglykKNAKILFLGLDNAGKTTLLRRLKDDRMVQHDPTLHPHAEELVLGNVRFKAFDLGGHKAVRKTW 81  Paramecium tetraur...
AAN31482    2 FVFDWFYNvlgylglyhKNAKILFLGLDNAGKTTLLHMLKDDRVAVHEPTLHPNSEELIIGKLRLRTFDLGGHETARRLW 81  potato late blight...
XP_667389   2 FVFSWFRDilswfglsnKSAKILFLGLDNAGKTTLLHMLKDDRIATHVPTLHPHSEELVIGKIKFKTFDLGGHETARRIW 81  Cryptosporidium ho...
CAI76569    3 FIINWIKKtlqslgllnKDARIVFLGLDNAGKTTLLRMLKDNRVGIHTPTLHPHSEQLSLEKVNLTAFDLGGHETARRVW 82  Theileria annulata
AAT06576    2 FLVDWFYGvlaslglwqKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQYPTSEELSIGRIKFKAFDLGGHQIARRVW 81  maize
XP_642388   2 FLVDWFYNmflwlgffkKEAKIVIIGLGNAGKTTLLHLLVTGSLKSHIPTLRPNAESFTYGNVNFKAYDLGGQQNLRFLW 81  Dictyostelium disc...
Q8MQT8      2 GFGDWFKSalsflglykKKATIVFVGLDNAGKSTLLAMLKNSATTTVAPTQQPTSQELVMGSIRFKTFDLGGHEVARQLW 81  Giardia intestinalis
NP_597349  19 FYEKVFQNfvks--lfgKPSSILFLGIDNAGKTTLVNKLKSDSTDVYMPTHHPSTSYIEIGNLKAQVIDLGGHTAARLAW 96  Encephalitozoon cu...
AAT09092    3 YFSGFFTGilklfgfysKEATVVLIGLDNAGKTTLQYKLRTGESYSFVPTQKPQEQEITIGNVKLQTWDLGGHKAVRKLW 82  Bigelowiella natans
CAI44555    2 FILNMFKSllgslglykKNAKILFLGLDNAGKTTLLRRLKDDRMVQHDPTLHPHAEELVLGNVRFKAFDLGGHKAVRKTW 81  Paramecium tetraur...
AAN31482    2 FVFDWFYNvlgylglyhKNAKILFLGLDNAGKTTLLHMLKDDRVAVHEPTLHPNSEELIIGKLRLRTFDLGGHETARRLW 81  potato late blight...
XP_667389   2 FVFSWFRDilswfglsnKSAKILFLGLDNAGKTTLLHMLKDDRIATHVPTLHPHSEELVIGKIKFKTFDLGGHETARRIW 81  Cryptosporidium ho...
CAI76569    3 FIINWIKKtlqslgllnKDARIVFLGLDNAGKTTLLRMLKDNRVGIHTPTLHPHSEQLSLEKVNLTAFDLGGHETARRVW 82  Theileria annulata
AAT06576    2 FLVDWFYGvlaslglwqKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQYPTSEELSIGRIKFKAFDLGGHQIARRVW 81  maize
XP_642388   2 FLVDWFYNmflwlgffkKEAKIVIIGLGNAGKTTLLHLLVTGSLKSHIPTLRPNAESFTYGNVNFKAYDLGGQQNLRFLW 81  Dictyostelium disc...
Feature 1                                                     ## #                            
1M2O_B     85 KDYFPEV-NGIVFLVDAADPERFDEARVELDALFNIAELkdVPFVILGNKIDAPn-----AVSEAELRSALgllnttg-- 156 baker's yeast
Q8MQT8     82 EQYVTNS-DGIVFLVDSADPSRFEESRRTLQELLDNHDLatTPILILSNKVDIQt-----AVSMETMVQSFgiqhlltgk 155 Giardia intestinalis
NP_597349  97 RDYFYDC-HGIVFIVDVHDVERFQEVREAYETVLSLEKR--APVVVLMNKIDLEghtpetAEADYQWKSWLsqetgienq 173 Encephalitozoon cu...
AAT09092   83 KQYYRTA-DGIVFVVDAADKSRLPEAKKVLNFILKEDALveTPIAILGNKSDKKe-----AVPMQKLQEELglpkl---- 152 Bigelowiella natans
CAI44555   82 KNYFPTV-DGIIYLVDSADSQRLKESRDELEQILNTAELakVPIVILGNKIDKPg-----AVPEEELRQALginvkqqin 155 Paramecium tetraur...
AAN31482   82 RDYFATV-DGVVFVVDALDRERFPESKRELDTLLGYDELanVPFLVLGNKIDVPr-----AASEDELRSALglyetygke 155 potato late blight...
XP_667389  82 KDYFATV-DAIVFLVDASDRSRFGETREELSNLLETPELqnVPFVILGNKIDIPm-----AASEDELRQSLglfehltyg 155 Cryptosporidium ho...
CAI76569   83 KQYCGNV-DGIVFIVDASDRSRFQESNEELRSLLSDEELtnKPFVVLGNKIDNRg-----AASEEELRTALslyandtyg 156 Theileria annulata
AAT06576   82 KDYYAKV-DAVVYLVDAVDKERFAESKKELDALLADDSLanVPFLILGNKIDIPy-----AASEEELRYYLglsnfttgk 155 maize
XP_642388  82 KQYVPDSkTIIVFMVDSSDYNSIIESKSEIHDILGDEHLsqSPLLILGSKCDAKg-----HHNRENLIDLLdirrfel-- 154 Dictyostelium disc...
Q8MQT8     82 EQYVTNS-DGIVFLVDSADPSRFEESRRTLQELLDNHDLatTPILILSNKVDIQt-----AVSMETMVQSFgiqhlltgk 155 Giardia intestinalis
NP_597349  97 RDYFYDC-HGIVFIVDVHDVERFQEVREAYETVLSLEKR--APVVVLMNKIDLEghtpetAEADYQWKSWLsqetgienq 173 Encephalitozoon cu...
AAT09092   83 KQYYRTA-DGIVFVVDAADKSRLPEAKKVLNFILKEDALveTPIAILGNKSDKKe-----AVPMQKLQEELglpkl---- 152 Bigelowiella natans
CAI44555   82 KNYFPTV-DGIIYLVDSADSQRLKESRDELEQILNTAELakVPIVILGNKIDKPg-----AVPEEELRQALginvkqqin 155 Paramecium tetraur...
AAN31482   82 RDYFATV-DGVVFVVDALDRERFPESKRELDTLLGYDELanVPFLVLGNKIDVPr-----AASEDELRSALglyetygke 155 potato late blight...
XP_667389  82 KDYFATV-DAIVFLVDASDRSRFGETREELSNLLETPELqnVPFVILGNKIDIPm-----AASEDELRQSLglfehltyg 155 Cryptosporidium ho...
CAI76569   83 KQYCGNV-DGIVFIVDASDRSRFQESNEELRSLLSDEELtnKPFVVLGNKIDNRg-----AASEEELRTALslyandtyg 156 Theileria annulata
AAT06576   82 KDYYAKV-DAVVYLVDAVDKERFAESKKELDALLADDSLanVPFLILGNKIDIPy-----AASEEELRYYLglsnfttgk 155 maize
XP_642388  82 KQYVPDSkTIIVFMVDSSDYNSIIESKSEIHDILGDEHLsqSPLLILGSKCDAKg-----HHNRENLIDLLdirrfel-- 154 Dictyostelium disc...
Feature 1                        ###                 
1M2O_B    157 ----sqriegqRPVEVFMCSVVm-rNGYLEAFQWLSQYI 190 baker's yeast
Q8MQT8    156 --ggsnlrsdqRPLEVFPCSVIn-rFGYTDGFKWLSKYL 191 Giardia intestinalis
NP_597349 174 edpergqvvkiFYVTITSGSANsitGPLARAFKWLEAMI 212 Encephalitozoon cuniculi GB-M1
AAT09092  153 -------ldayRKIKVFNTSVTe-gYGYPTAFEWIAANL 183 Bigelowiella natans
CAI44555  156 --nknikeidgRPVDVFMCSVAn-rVGYAEGFRWLSQFL 191 Paramecium tetraurelia
AAN31482  156 --argdkdasiRPIELYMCSVVr-rMGYADGFRWMAQFL 191 potato late blight agent
XP_667389 156 r-gekrgdngiRPVEVFMCSVVr-rMGYSEAFRWLSQFL 192 Cryptosporidium hominis
CAI76569  157 ---ksvksgrgRPVELFMCSIIk-kQGYAEALRWLSHFL 191 Theileria annulata
AAT06576  156 g-nvnladsnvRPLKIFMCSVVr-kMGYGEGFKWMSQYI 192 maize
XP_642388 155 ----glnnsnnVPFDLIMTSSIt-rYGITDMLNWLDKCT 188 Dictyostelium discoideum
Q8MQT8    156 --ggsnlrsdqRPLEVFPCSVIn-rFGYTDGFKWLSKYL 191 Giardia intestinalis
NP_597349 174 edpergqvvkiFYVTITSGSANsitGPLARAFKWLEAMI 212 Encephalitozoon cuniculi GB-M1
AAT09092  153 -------ldayRKIKVFNTSVTe-gYGYPTAFEWIAANL 183 Bigelowiella natans
CAI44555  156 --nknikeidgRPVDVFMCSVAn-rVGYAEGFRWLSQFL 191 Paramecium tetraurelia
AAN31482  156 --argdkdasiRPIELYMCSVVr-rMGYADGFRWMAQFL 191 potato late blight agent
XP_667389 156 r-gekrgdngiRPVEVFMCSVVr-rMGYSEAFRWLSQFL 192 Cryptosporidium hominis
CAI76569  157 ---ksvksgrgRPVELFMCSIIk-kQGYAEALRWLSHFL 191 Theileria annulata
AAT06576  156 g-nvnladsnvRPLKIFMCSVVr-kMGYGEGFKWMSQYI 192 maize
XP_642388 155 ----glnnsnnVPFDLIMTSSIt-rYGITDMLNWLDKCT 188 Dictyostelium discoideum

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap