2C71


Conserved Protein Domain Family
CE4_CtAXE_like

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cd10954: CE4_CtAXE_like 
Click on image for an interactive view with Cn3D
Catalytic NodB homology domain of Clostridium thermocellum acetylxylan esterase and its bacterial homologs
This family is represented by Clostridium thermocellum acetylxylan esterase (CtAXE, EC 3.1.1.72), a member of the carbohydrate esterase 4 (CE4) superfamily. CtAXE deacetylates O-acetylated xylan, a key component of plant cell walls. It shows no detectable activity on generic esterase substrates including para-nitrophenyl acetate. It is specific for sugar-based substrates and will precipitate acetylxylan, as a consequence of deacetylation. CtAXE is a monomeric protein containing a catalytic NodB homology domain with the same overall topology and a deformed (beta/alpha)8 barrel fold as other CE4 esterases. However, due to differences in the topography of the substrate-binding groove, the chemistry of the active center, and metal ion coordination, CtAXE has different metal ion preference and lacks activity on N-acetyl substrates. It is significantly activated by Co2+. Moreover, CtAXE displays distinctly different ligand coordination to the metal ion, utilizing an aspartate, a histidine, and four water molecules, as opposed to the conserved His-His-Asp zinc-binding triad of other CE4 esterases.
Statistics
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PSSM-Id: 200578
Aligned: 29 rows
Threshold Bit Score: 241.336
Created: 17-Aug-2010
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:based on similarity to other superfamily members

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1              ##                                                         #   #         
2C71_A        5 KLVALTFDDGPdn----vLTARVLDKLDKYnvKATFMVVGQRVnd---stAAIIRRMVNsGHEIGNHSWSYSGMa-nMSP 76  Clostridium ther...
Q52644      474 PMVAIAFDDGAsaakkgdPAYRIIDTMADNgfHATFFYIGSQIk-----tEEQVKYAHEkGMETANHTMTHPYLs-kLKP 547 Ruminococcus albus
ACZ98622    261 KLVALSFDDGPvnn--gsSATRIQDVMKKYnmHCTFFYWGITMgg----nTSEIQRAYElGCEVANHTNTHSYLt-nMSS 333 Clostridium sp. H1
EEQ56814    115 PMIAFTFDDGPft----kVDQRILDVLDAYggRATFFIVGSRVnd----yKDTLKRIHNsGSEIGNHTYNHKNLe-nLAP 185 Clostridiales ba...
ZP_02026115 195 KYVAFTFDDGPg-----iYTDKLLKALNKNnaKATFFVVGSCVns----hKTQLKNEYKlHMEIGSHTYNHSNLk-tLSV 264 Eubacterium vent...
ZP_06599211 193 PLVALTFDDGPdq----qIDQIIMDELEKVngRATFFVVGQRVqr----ySADVKSMAErGHEIGNHSWDHDIRlsrKGE 264 Oribacterium sp....
ZP_03633618 318 PMVALTFDDGPhp----kNTPRILETLRTYdgASTFFMVGTNVer----yPDVVRQVAEsGSEIASHTYDHPNLt-kLSG 388 Holdemania filif...
ZP_02025009  90 PMIALTFDDGPg-----qYTGKLLDALEKYnaRASFFMCGYSLkktdipvDELLKRMDAlGCDLGNHTMNHCALd-kVSK 163 Eubacterium vent...
ZP_05427494 280 PMIALTYDDGPg-----eHTDRLLKLFERVggACTLFQLGVNIenk-dpdHKILKKAVStGMELASHSYNHPNLl-tLSK 352 Eubacterium saph...
ZP_02420386 148 KLIAFSFDDGPar----eNTDRVVKALAKNhaRATFFMLGQNAsy----yPRLVKEVKDsGNEVAGHSWNHPQLt-kLGA 218 Anaerostipes cac...
Feature 1                                  #####                  #                             
2C71_A       77 DQIRKSIADTNAVIQKYAGt--tPKFFRPPNLET--SPTLFNNVDLVFVGG-LTANDWIpstTAEQRAAAVINGv----r 147 Clostridium ther...
Q52644      548 AEIRDEYEKCREKLKGIIGee-pSALMRLPYLDGg-GDTTRTLNDVALISCsVDTKDWNk-aTSDKIVDTIKKAkdngsl 624 Ruminococcus albus
ACZ98622    334 DQIKDEIGKVNNVLKGISGk--aSNLIRPPYLAT--NGTVTSSVDAPLVTCsIDSKDWDg-aSKDQIISNVLNNv----k 404 Clostridium sp. H1
EEQ56814    186 EEVISQVEMTNDAVEAVTGf--rPKLVRVPYGAF--KGQVSGLVSYPMIQWnIDTEDWSs-kDKDAILSTLLSQa----k 256 Clostridiales ba...
ZP_02026115 265 PQIKKELGSTNKVVKSVIGt--tPTLLRPPYGSY--NKTTGKYAGVPMIYWsVDTLDWKy-rNVNYVSSTILKEt----k 335 Eubacterium vent...
ZP_06599211 265 SYISSEFSKTDDAVRAVTGs--eTALIRLPGGTI--SDDVKKAVKKPLIYWtVDTDDWKs-rNAQSVVDIVMNQv----k 335 Oribacterium sp....
ZP_03633618 389 DRLNYQLSRVNELIGELTDqqvsVKTLRPPYGAV--SEQVREAADTPLILWsMDTLDWKt-rDAQKTISYVLEQv----e 461 Holdemania filif...
ZP_02025009 164 SKRKYEINGVNELVKKAVGy--nPKFLRPPYGSGirDAKVQKAAKMPIVCWsVDTLDWKt-kSKKKTVKSIMDSa----k 236 Eubacterium vent...
ZP_05427494 353 KKVIEQNHRTDEAFKKAVGf--aPRLYRPPYGAG--NGRTTKLFNKSAIFWsIDTYDWRh-kSAKHLAKFITSQkk---l 424 Eubacterium saph...
ZP_02420386 219 AGVCRQMQKMNYAISKVTGs--dVGLLRPPYGAI--DRMVKANINAPLILWsIDTLDWKt-lNTDATVNTILKQa----k 289 Anaerostipes cac...
Feature 1              #                                 
2C71_A      148 DGTIILLHDVQpephpTPEALDIIIPTLKSRGYEFVTLTEL 188 Clostridium thermocellum
Q52644      625 NGAIVLCHENYa---aTAEAMEEIVPWLKDEGWQVVTVSEL 662 Ruminococcus albus
ACZ98622    405 DGSIVLMHETYd---tTAAAVEYLVPELIKRGYTIVTVSEL 442 Clostridium sp. H1
EEQ56814    257 DGSIILMHDLYp---sTAEAFEAAVPQLVAQGYQLVTVSEM 294 Clostridiales bacterium 1_7_47_FAA
ZP_02026115 336 AWDIVLLHDIHq---tSVDGFIKALPTLKKRGYELVTVSEL 373 Eubacterium ventriosum ATCC 27560
ZP_06599211 336 DGDIILMHSLYl---sTAEACRTIIPELSARGYQLVTVSEM 373 Oribacterium sp. 'Oral Taxon 078' str. F0262
ZP_03633618 462 DGDIILMHDIHa---eSADAAVAIIPQLTAMGYQLVTVQEL 499 Holdemania filiformis DSM 12042
ZP_02025009 237 DGQIILMHDIHs---wSVDAAIEAIPKLQKKGYQIVSVSEM 274 Eubacterium ventriosum ATCC 27560
ZP_05427494 425 VSAVVLMHDIHk---sTVEASMKAVPSLKKKGYQLVTVSEL 462 Eubacterium saphenum ATCC 49989
ZP_02420386 290 DGDIVLMHDIHk---pSVAAAEKVLPLLKDRGFEVCTVSEL 327 Anaerostipes caccae DSM 14662

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