Conserved Protein Domain Family
AmyAc_OligoGlu_like

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cd11331: AmyAc_OligoGlu_like 
Alpha amylase catalytic domain found in oligo-1,6-glucosidase (also called isomaltase; sucrase-isomaltase; alpha-limit dextrinase) and related proteins
Oligo-1,6-glucosidase (EC 3.2.1.10) hydrolyzes the alpha-1,6-glucosidic linkage of isomalto-oligosaccharides, pannose, and dextran. Unlike alpha-1,4-glucosidases (EC 3.2.1.20), it fails to hydrolyze the alpha-1,4-glucosidic bonds of maltosaccharides. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.
Statistics
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PSSM-Id: 200470
Aligned: 16 rows
Threshold Bit Score: 667.876
Created: 16-Oct-2011
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
active sitecatalytic site
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                #  #                    
YP_001276270   9 LWWQRGVIYQIYPRSFQDsnGDGVGDLRGIRSRLDYLVDLGVDAIWLSPIFPSPMADFGYDVSDYCDIHPLFGTLTDFDT 88  Roseiflexus sp....
ZP_01747548   15 EWWKTGIIYQIYPRSFQDsdGDGVGDLKGIEGRLDYLVDLGIDAIWISPIFPSPMADFGYDVSDYRGIDPMFGTLEDFDR 94  Sagittula stell...
AAF10944      39 KWWQSGIIYQIYPRSYQDsnGDGVGDLPGITARLPYVASLGVQAVWLSPIFKSPMRDFGYDVADYCDIDPVFGTLEQFDA 118 Deinococcus rad...
BAB18518       2 SWWQRAVIYQVYPRSFQDtnGDGVGDLEGIRRRLPYLKSLGVDALWLSPFYKSPMKDFGYDVADYCDVDPVFGTLQDFDR 81  Bacillus flavoc...
YP_003125391   7 KWWQTGVIYQVYPRSFQDsnGDGVGDLNGVISRLDYLQWLGIDAVWLSPIYPSPMADFGYDIADYTGIHPLFGNQEDFDK 86  Chitinophaga pi...
CAD72944      55 RWWETGVIYQIYPRSFQDsnADGVGDLAGIKQRLDYLVRLGVDAVWLSPIYPSPMFDFGYDVSDYCGIDPLFGDLTAFDR 134 Rhodopirellula ...
YP_113423      9 AWWQTGIIYQIYPLSFQDsdGDGRGDLPGILHRLDYLADLNIAAVWLSPVFASPMRDFGYDVADYTSIHPWFGTLSDFDR 88  Methylococcus c...
YP_003382360   5 DWWRDAVIYQIYPRSFQDtaGNGVGDLAGIIARLPYLSWLGVDALWMCPFYRSPQADFGYDITDHTAVDPLFGSLRDFDD 84  Kribbella flavi...
YP_001770302  23 AWWQERVLYQIFVPSFQDadGDGFGDLAGLISRLDHLSDLGIGAVWLTPIYATPFEDAGYDVTDFTQVGARFGDLPTFDR 102 Methylobacteriu...
YP_001171038   8 PWWMGATVYQIYPRSFADsnGDGIGDLQGVLRHLDYLKRLGVDALWLSPIFRSPMADAGYDISDYCDIDPLFGSLADIDR 87  Pseudomonas stu...
Feature 1                        #  #                                         #                  
YP_001276270  89 LVADAHRRNLKVILDFVPNHTSDQHPWFIESRSSrsnPKRDWYIWRDPapdggpPNNWLSYFG-GSAWEYDAtTGQYYLH 167 Roseiflexus sp....
ZP_01747548   95 LVAATHGRGMKLILDFVPSHTSDQHPWFLDARSSrtsAKRDWYVWRDAkadgspPTNWISEFG-RPAWTWDEgTGQYYLN 173 Sagittula stell...
AAF10944     119 LVAEAHRLGLKVMLDYVPNHTSSDHAWFQEALTGkasAKRDWYVWRDPapdgglPNNWKSFFG-GPAWTLDEaSGQYYLH 197 Deinococcus rad...
BAB18518      82 LLEEAHALGLKVLVDLVPNHTSSEHPWFLESRASrnsPKRDWYIWKDPapdggpPNNWQSFFG-GPAWTLDEaTGQYYLH 160 Bacillus flavoc...
YP_003125391  87 LLKEVHDRGMKLLLDLVPNHTSNQHPWFLESRSSldnPKRDWYIWHDPlpdggaPNNWLSVFG-GEAWEWDPsTQQYYYH 165 Chitinophaga pi...
CAD72944     135 LLTAMHSRGLKLLMDFVPNHSSDQHPWFVQSRSSrdnPKRDWYIWRDAsadggpPNNWISDFG-GSSWQWDAtTRQYYLH 213 Rhodopirellula ...
YP_113423     89 LLAGLHGRGIKLILDLVPNHTSDRHPWFLESRSSrdnPRRDWYLWRDPapgggpPNNWLSFFG-GPAWTYDEtTGQYYLH 167 Methylococcus c...
YP_003382360  85 LVKAAHQAGLRVIVDFVPNHTSIEHHWFVASRSStasPYRDWYLWADPapdggpPNNWRSVTG-GSAWTHDArTDQYYLH 163 Kribbella flavi...
YP_001770302 103 LLAGLHARDIRLILDFVPNHTSDRHPWFRESRASrasPRRDWYVWRDPgpegsaPNNWLSRFG-GSAWTHDArTDQSYYH 181 Methylobacteriu...
YP_001171038  88 LIAEAHARDIRVLLDFVPNHTSDEHPWFVESRSSrdnPKRDWYIWRDQ------PNNWRAALGaGSAWTWDEhTQQYYLH 161 Pseudomonas stu...
Feature 1         #   #                               #                                          
YP_001276270 168 LFLKEQPDLNWRNPQVQAAMLDAMRFWLDRGVDGFRVDVMWLMIKDDQFRDNPpnpawkpgmmphm----rlletysadq 243 Roseiflexus sp....
ZP_01747548  174 IFLSEQPALNWRNPEVQAEMLDTLRFWYARGVDGFRVDAITHIAPDPDKGDHPddpdwrehmdpsy----rylkvhskhq 249 Sagittula stell...
AAF10944     198 QFLPSQPDLNWRNPDVRAAMFDVLRFWMRRGVDGFRVDVIWLLAEDERFLDEPenpdadrevvggqiehstllhiytqdq 277 Deinococcus rad...
BAB18518     161 LFLPEQPDLNWRNPEVREAIKEVMRFWLRRGVDGFRVDVLWLLGKDPLFRDEPgsplwrpglpdra----rhehlytedq 236 Bacillus flavoc...
YP_003125391 166 AFLKEQPDLNWRNPEVQAAMFDVMRYWLQKGVDGFRVDVMWHMIKDKQLRNNPtnpdyeahmgtys----qqlpvystdq 241 Chitinophaga pi...
CAD72944     214 AFLPQQPDLNWRHPELREAMMNVLRFWLDRGVDGFRMDVLWHIVKDAALRDNPinpdwtsdrtqrd----qliqlhstdq 289 Rhodopirellula ...
YP_113423    168 QFTPEQPELNLRHPAVLEAMLEVMRFWLDRGVDGFRVDVPWLLIKDAGFRDEPenpawdgidphgr-----llhihtahq 242 Methylococcus c...
YP_003382360 164 SFLPTQPDLNWRNPAVVKAMHDVLRFWLDRDVDGFRIDMVDYLIKDQQLRDEPldnaggyqpat---------asyqlnq 234 Kribbella flavi...
YP_001770302 182 AFLPSQPDLNWRNPEVRRAMHEVMRFWLRRGVDGFRIDAAAVLAEDAALRDDPpnpdfdgdtpppe----rfrrlrtdsq 257 Methylobacteriu...
YP_001171038 162 LFLAKQPDLNWRNPEVVAAMHDVLRFWLDRGIDGFRIDVIHCTGKDPSFADDPrcqagqpls-------------dfndq 228 Pseudomonas stu...
Feature 1                                    #                                                   
YP_001276270 244 pdvHTIVAMMRRVLDSY----DERMMVGEIYl--PYDRLMRYYGtpeapEAHLPFNFALVl---lPWDAQTIAQTIALYE 314 Roseiflexus sp....
ZP_01747548  250 pdgLDFVRMMRRVTDEF----PDRVLIGETDg--KLHDVMAYYGed-fdAFHLPFNFTLQg---aPWEMPEIVRRVEAYE 319 Sagittula stell...
AAF10944     278 petHDYVREMRRVIDEF----DDRMMVGEIYl--PLDKLLPYSGtpeapMVHLPFNFHLIl---lPWDAQTIRQFADEYD 348 Deinococcus rad...
BAB18518     237 petYAYVREMRQVLDEFsepgRERVMVGEIYl--PLPRLVRYYAa----GCHLPFNFSLVteglsDWRPENLARIVETYE 310 Bacillus flavoc...
YP_003125391 242 pevHEVVRNMRAVMEEFd---GDRVMIGEIYl--PLQQLMAYYGvd-nkGAHLPFNFQLLs---lPWQSASLAVAIDQYE 312 Chitinophaga pi...
CAD72944     290 peaHSIAAEFRTLADNY----GDRVLIGEISl--PNDRLARWFGteerpEVHLPTNFHLIe---cEWNAPTLRRVIANYE 360 Rhodopirellula ...
YP_113423    243 pelHRIIRAMRAVVDGYp---GERVLIGETNv--PEEELVKYYGaa-rdEFHLPFNFRLIh---aPWDARKIRQMVETYE 313 Methylococcus c...
YP_003382360 235 petIDLLRSFRALTDGYg---HGRVLIGEVEyglPMPRLTSYYGnd--dALQLPINFWLLf---lPWTAQALQRFITDYE 306 Kribbella flavi...
YP_001770302 258 vvtLDYLAELRRVVEEF----PDRVLLGEVDt--APDKLPGFYGeg-trRLHLPLNYRLLs---vPWKAAALAGAVDAYL 327 Methylobacteriu...
YP_001171038 229 pysHEVLRGLRKLVDSYp---GDRMLVGEVNi-rSTERVLQYYGag--dELHLAFNFNPLd---aPWDPVLFRTCIREVE 299 Pseudomonas stu...
Feature 1                        ##                                                              
YP_001276270 315 ALlp-pFAWPNWVLGNHDQPRIASRV--GEAQARVAAMLLLTLRGTPTIYYGDEIGMHNVPIPPDRvqdpfek------n 385 Roseiflexus sp....
ZP_01747548  320 AAlp-gGAWGNWVLGNHDCTRIASRA--GPAQAAVAATLLLTLRGTPTLYQGDELGMESAVIPPEAvqdpwek------q 390 Sagittula stell...
AAF10944     349 AAsnaaGAWPNWVLGNHDQHRFKTRV--GAAQYRVAQTLLLTLRGTPTVYYGDEIGMENVPVPPEKmvdpsglq-----q 421 Deinococcus rad...
BAB18518     311 GLls-rWDWPNWVLGNHDQPRLASRL--GEPQARVAAMLLFTLRGTPTWYYGDELALPNGLIPPEKvqdpaalrqrdrep 387 Bacillus flavoc...
YP_003125391 313 GAlp-nQGWPNWVLSNHDQHRIASRV--GQLQARVAAMLLLTLRGTPTIYYGDEIAMRNVAIPFEEvqdpqgl------n 383 Chitinophaga pi...
CAD72944     361 ASlp-nFGWPNWVLGSHDAPRIAARL--GDAQSRVAAMLLLTLRGTPTLYQGDEIGIGEVPIPRDQirdpqdl------r 431 Rhodopirellula ...
YP_113423    314 AIlp-qRASPAWVLGNHDQPRIASRF--GPDAARVATMLLLTLRGTPTCYYGDELGMENGVIPRDRirdpqaln----qp 386 Methylococcus c...
YP_003382360 307 AGlp-aAAWPNWVIGSHDISRAASRL--GAARVRSALLVLLTLRGTPFLYYGDELGLPDAELQARDkrdpwv-------- 375 Kribbella flavi...
YP_001770302 328 DAlp-pGAWPNWVLGSHDKARIASRV--GPAQARVAALMLLTLPGTPMLFAGDEIGMPNVPVPVDRmrdpfer------l 398 Methylobacteriu...
YP_001171038 300 HWlqpaGAWPTWVLSNHDNVRQRSRYqgSERAARAAAVLLLTLRGTPFIYQGEELGLEDAHIADEArid----------- 368 Pseudomonas stu...
Feature 1                                                                                     
YP_001276270 386 vpgeghGRDPQRTPMQWDASe-YAGFSKVq--PWLPVADDYrqrNVATQRNVPHSMLSLYRRLLALRRSEPALSIGS 459 Roseiflexus sp. RS-1
ZP_01747548  391 vpgrglGRDPARTPMPWGPGq-AHGFSEGd--PWLPVFVPAa-gDATTQRAEVGSLLNYVRALIALRRDTPALTLGS 463 Sagittula stellata...
AAF10944     422 pdspdaGRDPERTPMQWEAAp-GAGFTAAgtePWLPLTDNFaqvNVQVQEQDSQSDLNYFRALTRLRQEQPALVGGS 497 Deinococcus radiod...
BAB18518     388 tayhtlGRDPERTPMPWDASp-YGGFSTVe--PWLPLNPDYrtrNVAVQEQDPRSMLHLVKRLIALRKDPDLLYGAY 461 Bacillus flavocald...
YP_003125391 384 mpdknlSRDPSRTPMQWDDSp-NAGFTSGk--PWLRLSKTWqrvNVSTQQHDPYSMLTLYRELIDLRRKEPSLSSGD 457 Chitinophaga pinen...
CAD72944     432 qpelniGRDRSRTPMPWDDSa-FAGFSVNd--TWLPLNPDWrqrNVATQEADPQSILNLYRTLLSLRRSHAALSNGD 505 Rhodopirellula bal...
YP_113423    387 gisgvfNRDEARTPLPWDTSp-NAGFAPEgvdPWLPLGEDWpmrNVARQAADPRSMLALFRALTRLRQCHPALSHGS 462 Methylococcus caps...
YP_003382360 376 qadasiGRDPARTPMPWTADlpHAGFCAAeaqPWLPIGSNHqglDVETQLQDPGSTLHLTRDLLTLRRTHRALRAGS 452 Kribbella flavida ...
YP_001770302 399 vpgyglSRDPSRVPLPWTAGp-GGGFSAVe--PWLPAPCPPpvaPVAAQARDPASLLSLYRAVIALRRRRPEFRAGG 472 Methylobacterium s...
YP_001171038 369 ----pgGRDGCRAPIPWLAEppHDWNGTS---PWLPFPPDAdelAAERQVGAANSMFALYQRLLAARKASPALLHGD 438 Pseudomonas stutze...

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