Conserved Protein Domain Family

cd08687: C2_PKN-like 
C2 domain in Protein kinase C-like (PKN) proteins
PKN is a lipid-activated serine/threonine kinase. It is a member of the protein kinase C (PKC) superfamily, but lacks a C1 domain. There are at least 3 different isoforms of PKN (PRK1/PKNalpha/PAK1; PKNbeta, and PRK2/PAK2/PKNgamma). The C-terminal region contains the Ser/Thr type protein kinase domain, while the N-terminal region of PKN contains three antiparallel coiled-coil (ACC) finger domains which are relatively rich in charged residues and contain a leucine zipper-like sequence. These domains binds to the small GTPase RhoA. Following these domains is a C2-like domain. Its C-terminal part functions as an auto-inhibitory region. PKNs are not activated by classical PKC activators such as diacylglycerol, phorbol ester or Ca2+, but instead are activated by phospholipids and unsaturated fatty acids. The C2 domain was first identified in PKC. C2 domains fold into an 8-standed beta-sandwich that can adopt 2 structural arrangements: Type I and Type II, distinguished by a circular permutation involving their N- and C-terminal beta strands. Many C2 domains are Ca2+-dependent membrane-targeting modules that bind a wide variety of substances including bind phospholipids, inositol polyphosphates, and intracellular proteins. Most C2 domain proteins are either signal transduction enzymes that contain a single C2 domain, such as protein kinase C, or membrane trafficking proteins which contain at least two C2 domains, such as synaptotagmin 1. However, there are a few exceptions to this including RIM isoforms and some splice variants of piccolo/aczonin and intersectin which only have a single C2 domain. C2 domains with a calcium binding region have negatively charged residues, primarily aspartates, that serve as ligands for calcium ions.
PSSM-Id: 176069
Aligned: 4 rows
Threshold Bit Score: 143.683
Created: 29-Mar-2010
Updated: 2-Oct-2020
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
Format: Row Display: Color Bits: Type Selection:
6225859   364 TRSSFMSRtsksksgss-------------------------------------rnllktddlSNDVCAVLKld-nTVVG 405  human
ABB22769   57 LQVRLLGCvglldvvpgrsrgspvtlpcyspgeg---rpfklslysrnsshslkipgktdelsSGVSAVLKLentvVGQT 133  milkfish
Q63433    329 LEVRVVGCknlpetipwspppsvgasgtpdsrtpflsrparglynrsgslsgrsslkgeaensTEVSTVLKLdntvVGQT 408  Norway rat
XP_697542 320 LEVRLMGCqdllesvpgrcrvsnmssapgspsds----kslrmrtglstrstngkttktdelsSEISAVLKVdnkiVGRT 395  zebrafish
6225859   406 QTSWkpisNQSW-DQKFTLEldrsrELEISVYWRDwrs---------------------------lcAVKFLRLedfld- 456  human
ABB22769  134 SWKTv--gEQAW-NQTFTVDlersrEMEIAVYWRDyrslcavkylkleefldnqrheiqldlepqgrLLAEVTFfnpvie 210  milkfish
Q63433    409 AWKPc---GPNAwDQSFTLElerarELELAVFWRDqrglcalkflkledfldnerhevqldmepqgcLVAEVTFrnpiie 485  Norway rat
XP_697542 396 HWRPl--sKDAW-NQSFSIElersrELEIAVYWRDwrslcavkflrled----fldnqrhgmclylePQGTLFTevrfin 468  zebrafish
6225859   457 ------------------------------nqrhGMCLYL 466  human
ABB22769  211 rgprlqrqrkvf----skqqgkaflkarqmnvdiATWVRL 246  milkfish
Q63433    486 riprlqrqkkif----skqqgqtfqrarhmnidvATWVRL 521  Norway rat
XP_697542 469 pvierhpklqrqkrifpkekgknflraaqmnmnfATWGRL 508  zebrafish
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