Conserved Protein Domain Family
C2C_MCTP_PRT_plant

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cd04019: C2C_MCTP_PRT_plant 
C2 domain third repeat found in Multiple C2 domain and Transmembrane region Proteins (MCTP); plant subset
MCTPs are involved in Ca2+ signaling at the membrane. Plant-MCTPs are composed of a variable N-terminal sequence, four C2 domains, two transmembrane regions (TMRs), and a short C-terminal sequence. It is one of four protein classes that are anchored to membranes via a transmembrane region; the others being synaptotagmins, extended synaptotagmins, and ferlins. MCTPs are the only membrane-bound C2 domain proteins that contain two functional TMRs. MCTPs are unique in that they bind Ca2+ but not phospholipids. C2 domains fold into an 8-standed beta-sandwich that can adopt 2 structural arrangements: Type I and Type II, distinguished by a circular permutation involving their N- and C-terminal beta strands. Many C2 domains are Ca2+-dependent membrane-targeting modules that bind a wide variety of substances including bind phospholipids, inositol polyphosphates, and intracellular proteins. Most C2 domain proteins are either signal transduction enzymes that contain a single C2 domain, such as protein kinase C, or membrane trafficking proteins which contain at least two C2 domains, such as synaptotagmin 1. However, there are a few exceptions to this including RIM isoforms and some splice variants of piccolo/aczonin and intersectin which only have a single C2 domain. C2 domains with a calcium binding region have negatively charged residues, primarily aspartates, that serve as ligands for calcium ions. This cd contains the third C2 repeat, C2C, and has a type-II topology.
Statistics
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PSSM-Id: 175986
View PSSM: cd04019
Aligned: 23 rows
Threshold Bit Score: 187.876
Threshold Setting Gi: 15228607
Created: 30-Dec-2004
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
putative Ca2+
Feature 1:putative Ca2+ binding site [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                        #           #                                                # # 
gi 226495161  203 YVRVNVIEARDIAFADKa------rVGEVFVRSRIaaqVHKTRTCVar-lpTCGWNEDHMFVAAEPFedHLILSVEDRVk 275
gi 3047119    194 KKNYEIMLADVIIVSDKs------rVPEVFVRVKVgnqMLRTKFPQrs--nNPKWGDEFTFVVAEPFedNLVLSVEDHTa 265
gi 3287696    212 YLRIHVMEAQDLVPSDKg------rVPDAIVKIQAgnqMRATRTPQmr-tmNPQWHEELMFVVSEPFedMVIVSVDDRIg 284
gi 6850863    200 YVRVTIVSGHDLISKDKn------kTPSVYVTATLgkvALKTKVSSg---tNPSWNQDLIFVASEPLegTVYIRLIDREd 270
gi 55733914   235 YLRVAAIGAQDLVPLDAs------rPANFCVKLQLagqVRRTRPGAppgtlNPIWNEEFMFVVSEPFdePLFVTVEDRVg 308
gi 115461524  452 YLKVVAIAAQDLIPAEKgr-----pLAPSIVKIQLggqTRRTRSQGs---aNPMWNEEFLFVAAEPFdePLVVTVEERVa 523
gi 148906480  179 YVRVNVIEAQDLIPSDKt------kFPDFQVKVQLgnqTGKTKPAVpsrnmSPFWNEELLFVAAEPFedHLILTVEDRLg 252
gi 223975463  288 YLRVGVLEAQDVVPPGAcatpdkgrHAEVFAKVQVggtVLRTRPCTtrgptNLAWNEELVFAVAEPFedPAVLIIEARVh 367
gi 224100519  192 YLKIKVNGAQDLVVSDPn------rKPEVYVKATLgnkVLKTKVSKnk-gvNPSWNEELMFVVAEPFedALILSVEDDKg 264
gi 224125320  200 YLRVQVIAAQDLAPSDKn------rKPEAYIKAVLgnlVLRTTVSKdk-npNPTWNEEVMFVAAEPFddHLILSVEDKMg 272
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1             #                                                                           
gi 226495161  276 vdkEEVIGHVHIPFkeferr------wdarpiRPRWFNLVrpega---------akidkFSAKICVRLCleGGYRVLTEP 340
gi 3047119    266 pnrDEPVGKAVILMndiekrkgtsdtyvvakyGHKWVRSRtvinsmnpk-yneqytwevFDPATVLTICvfDNAHFAAGD 344
gi 3287696    285 pgkDEILGRVFIPVrdvpvrq-----evgkmpDPRWFNLQrhsmsmee---enekrkekFSSKILLRVCieAGYHVLDES 356
gi 6850863    271 eqhEGCIGTLKKKLtemtpl------kvpssaPALFYDIEmptevkp------agdsrrFASRLKMKLAtdQAYHVAEEC 338
gi 55733914   309 pgrDEPLGRIMLPLnaamprh----dhfgkpvEPRWYSLArpsddp-------dkkegkFASKIQLRMSldFGYHVLDES 377
gi 115461524  524 agrDEPVGRVIIPVaapyvpr----ndlaksiEAKWFSLSraltadeaaaaeatklkssFASKIHLRLSleTAYHVLDES 599
gi 148906480  253 pnkEEIMGRAIIPLnlvdkr------ldhktaIPRWFSLEkpavaaaeg--dskkkevkFASRIFLRLSldGGYHVLDES 324
gi 223975463  368 pgkDEIVGRALLPLtifekr------ldcrpvQSQWFSLEhfgrp----------apavFAGRVHLRACleGAYHVMEEP 431
gi 224100519  265 dnmVDYLGKCVKPVhk--------------llRDCCLLFQgpm--------------ekFSSKLRVTIYldGVYHVFDEP 316
gi 224125320  273 ankEVCLGRSVIPLhqvekr------lmpqaiGAQWINLEkyvaege------ektevkFASRLHLRIFldGLYHVFDEP 340

                  ....*....
Feature 1                  
gi 226495161  341 VHYLSDVRP 349
gi 3047119    345 GGNKRDQPI 353
gi 3287696    357 THFSSDLQP 365
gi 6850863    339 TQYSSDNRA 347
gi 55733914   378 TYYSSDLQP 386
gi 115461524  600 THYSSDLQP 608
gi 148906480  325 THYSSDLRP 333
gi 223975463  432 TMYASDTRP 440
gi 224100519  317 ALFSTDLKA 325
gi 224125320  341 TYYSSDLRA 349

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