2W5A,2WQM


Conserved Protein Domain Family
STKc_Nek

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cd08215: STKc_Nek (this model, PSSM-Id:173755 is obsolete and has been replaced by 270855)
Click on image for an interactive view with Cn3D
Catalytic domain of the Protein Serine/Threonine Kinase, Never In Mitosis gene A-related kinase
Serine/Threonine Kinases (STKs), Never In Mitosis gene A (NIMA)-related kinase (Nek) family, catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The Nek family is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. The Nek family is composed of 11 different mammalian members (Nek1-11) with similarity to the catalytic domain of Aspergillus nidulans NIMA kinase, the founding member of the Nek family which was identified in a screen for cell cycle mutants that were prevented from entering mitosis. Neks contain a conserved N-terminal catalytic domain and a more divergent C-terminal regulatory region of various sizes and structures. They are involved in the regulation of downstream processes following the activation of Cdc2, and many of their functions are cell cycle-related. They play critical roles in microtubule dynamics during ciliogenesis and mitosis.
Statistics
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PSSM-Id: 173755
Aligned: 60 rows
Threshold Bit Score: 295.562
Created: 5-Jul-2006
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1             ## ##   #             # #                              #                 
2W5A_A       7 DYEVLYtIGTGSYGRCQKIRRks-dGKILVWKELDygsmteaekqMLVSEVNLLRELkHPNIVRYYDRIIdrt------n 79   human
CAJ04405    43 KYRVRRaIGRGAFSTVWLLINnk-tGKTVVGKLSDashsssackaFAEAEVENMRCCsHPNIISLIETFEag-------- 113  Leishmania major
XP_822838   32 KYWIKRvLGSGATGTVLCAKRts-dGETFAVKVVDlegmnegdknRAQAEVHCLMNCdFFSILKCHEDFAkkdpqnevnv 110  Trypanosoma bruc...
AAX79478    13 RYAIEKaLFLGNTTDVFKVREvg-tDRPYVLKQMSllpmgaderkRVLQEICVMSGVdHPNIVKFRESFSgn-------- 83   Trypanosoma brucei
AAZ14739    67 MYVLTTlVGRNPTTAAFVATRgsdpSEKVVAKFVMlndd--rqatYARSELHCLAACkHFGIVKHFDDFKsd-------- 136  Leishmania major...
XP_638809   11 KYLIKSqIGSGSYGNTFKVTHke-sGQVFCKKSIQvnnq--enieKVLEEGKILTVMdHVNVVKLNDSFFen-------- 79   Dictyostelium di...
XP_804294   29 KYTYVRyLAHGSSGETWKVLDvs-dGTPYALKITSlknyrepkllRVRTEVRCLESIdHFACIRLHEKLEte-------- 99   Trypanosoma cruz...
XP_666371   20 NYRIIEyVGKGQFGNTYKVENti-dYKIWLAKCIDlsqmdeddkkRSLQEAEIMKSInHPYVIKCHESFIhdd------- 91   Cryptosporidium ...
CAK91365    39 QFTILNeLGKGSYGVVYKVKSsm-dGNIYVLKKINlthlkpkhqaEALKEAQLLRKLkHPNVITYYMSFIeq-------- 109  Paramecium tetra...
CAK91253     2 DYEIIDqIGKGSYGVIYKVKIr---SQIQVLKEISlknlslkqqnEAYREAQIMHTLnHPNIVSYYNSQLrk-------- 70   Paramecium tetra...
Feature 1             ###   #                                                            # #   
2W5A_A      80 ttLYIVMEYCEGGDLASVITkgtk--------erqYLDEEFVLRVMTQLTLALKECHrrsdgghtVLHRDLKPANVFLDg 151  human
CAJ04405   114 ekSLYILEYANAGDLQAQVDtraqpppgtsdgtsiPYREDEVLVIFAQLGLAIRYLHdr-----rIMHRDLKTSNVLLTr 188  Leishmania major
XP_822838  111 lmIALVLDYANAGDLRQEIKsrak--------asrTFREHEAGLLFIQVLLALHHVHsr-----hMIHRDIKSANILLCs 177  Trypanosoma bruc...
AAX79478    84 tsVNIIMECCKCTLEEIIMLqqe---------egqPFPEEAIIEWMVELLSGLAHLHsr-----rVVHRDIKTSNIFVTe 149  Trypanosoma brucei
AAZ14739   137 dkLLLIMEYGSGGDLNKQIKqrlk--------ehlPFQEYEVGLLFYQIVLALDEVHsr-----rMMHRDLKSANIFLMp 203  Leishmania major...
XP_638809   80 gnYVIIMEFAENGDLFQKIEnqkr--------sgkPFSDFEIMHYFCQLVIALNYIHsq-----nIIHRDIKPKNIVLSs 146  Dictyostelium di...
XP_804294  100 shFFFVMEYCDAGDLRYQMHdg------------gKLLEHQIVHILLQLVLALHYLHtk----kkILHRDLKPANVLLKt 163  Trypanosoma cruz...
XP_666371   92 vyLVIIMEYCERGDIGAVIDscis--------kgtYLPEEKILYWCAQLAAGLYYLHne----crIIHRDIKPSNIFIRe 159  Cryptosporidium ...
CAK91365   110 dnLCIIMEYAEGGDLQKLLKdyke--------rrkFMQEETIWEMSRELSSALQHLHen-----nIIHRDIKTLNVFLTk 176  Paramecium tetra...
CAK91253    71 dkLNIFMEYCEDDLHNYIYRn-------------pSPAENQVWQWTIQLLEGLEYLHsq-----kIVHRDIKPNNILMKs 132  Paramecium tetra...
Feature 1                                   #                                                  
2W5A_A     152 -----------------kQNVKLGDFGLARILNhdt--sfaKTFVGTPYYMSPEQMNRMsYNEKSDIWSLGCLLYELcaL 212  human
CAJ04405   189 -----------------sGLIKLGDFGFSRQYQesvsgevgKTFCGTPYYLAPEMWQRQsYSYKADIWSLGVIMYELlaL 251  Leishmania major
XP_822838  178 -----------------nGLIKLGDFGFTKMYSatvsddvgKTFCGTPYYVAPEIWRRKpYSKKADLFSLGVLLYELltL 240  Trypanosoma bruc...
AAX79478   150 -----------------kNHLKLGDFGVCTVLTsas--iatNSMVGTPLYFSPEVCAGDaYDVRSDVWSLGVVFYEMctL 210  Trypanosoma brucei
AAZ14739   204 -----------------tGIIKLGDFGFSKQYSdsvsldvaSSFCGTPYYLAPELWERKrYSKKADMWSLGVILYELltL 266  Leishmania major...
XP_638809  147 sdsgsgsgsgsgssndsiPLLKIADFGVSKLMSetd--lyaNTTAGTPQYVSYEICNKKpYTNKTDIWSLGVVLYELmtL 224  Dictyostelium di...
XP_804294  164 -----------------tGLLKLGDFGLSNAYDtisg-dvgLTICGTPAYVAPELWRNErYGAAADIWSLGVILYEAmtG 225  Trypanosoma cruz...
XP_666371  160 -----------------nGDLVIGDFGISRIMLsvtm-pftLTSIGTPQYMSPEMCENKpYTYKSDIWSFGCVLYELtcL 221  Cryptosporidium ...
CAK91365   177 -----------------dKHVKLGDLGVSKIFNsdt--alqGTRVGTPLYLSPELVQHQpYDYKVDIWALGCVVFYMaaL 237  Paramecium tetra...
CAK91253   133 ------------------NILKISDLGESKSLIstq--qlsTTKVGTPLYLAPELIRNRpYDNKIDIWALGCVIYFVcqG 192  Paramecium tetra...
Feature 1                                                                    
2W5A_A     213 MPPFTAfsqKELAGKIREGk--FRRIpy-rYSDELNEIITRMLNLkDYHRPSvEEILENPLI 271  human
CAJ04405   252 KKPFQAtnlSELMEMVTRQg-sFDPLpedrYSSDMISLVNQMLRVdPSERPSiKDILALPLF 312  Leishmania major
XP_822838  241 RRPFDGesmRDVMNRTLSGq--YDPLpp-eTSPEMAEIVSLLLSSdPKQRPCsGKLLDMPIC 299  Trypanosoma brucei TREU927
AAX79478   211 RRPFEAehlPGLLQQVLTRd--VAPFdt-gLDTRLEEIVLRMLRKdPKERPTsQDLIDNHLV 269  Trypanosoma brucei
AAZ14739   267 HRPFKGpsqREIMQQVLYGk--YDPCpc-pVSSSMQALLDPLLSKnPTERPTtQQLLQTEFL 325  Leishmania major strain Friedlin
XP_638809  225 SLPFDGr-kETVMRNIQTEstiFKPVn--hPNEELSSLLFKLLNKnPESRFStQQILEQVFI 283  Dictyostelium discoideum AX4
XP_804294  226 RHPFEGtkyEELRHKVLHEe--VPPIkt-sHSVELKRIVYALLRKsSVERPDtTQLLREPLL 284  Trypanosoma cruzi strain CL Brener
XP_666371  222 KPPFSGdslLSLAWKISFQe--IEPLps-cYSSNLFKLIQSLLSRdPILRPDpLEILNNESF 280  Cryptosporidium hominis TU502
CAK91365   238 EPPFQGenlIALGYSIVNRa--PKALpp-qYSTRLSQFIWKLLEKiPALRPSiSEVNTIYFQ 296  Paramecium tetraurelia
CAK91253   193 DPPFGGsniISLGYNIVNKs--PKPIns-kYSRRLQSLIFKLLNKvPEFRPTaAQALQQIRD 251  Paramecium tetraurelia

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