Conserved Protein Domain Family
STKc_MPK1

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cd07857: STKc_MPK1 
Catalytic domain of the Serine/Threonine Kinase, Fungal Mitogen-Activated Protein Kinase MPK1
STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. This subfamily is composed of the MAPKs MPK1 from Saccharomyces cerevisiae, Pmk1 from Schizosaccharomyces pombe, and similar proteins. MPK1 (also called Slt2) and Pmk1 (also called Spm1) are stress-activated MAPKs that regulate the cell wall integrity pathway, and are therefore important in the maintainance of cell shape, cell wall construction, morphogenesis, and ion homeostasis. MPK1 is activated in response to cell wall stress including heat stimulation, osmotic shock, UV irradiation, and any agents that interfere with cell wall biogenesis such as chitin antagonists, caffeine, or zymolase. MPK1 is regulated by the MAP2Ks Mkk1/2, which are regulated by the MAP3K Bck1. Pmk1 is also activated by multiple stresses including elevated temperatures, hyper- or hypotonic stress, glucose deprivation, exposure to cell-wall damaging compounds, and oxidative stress. It is regulated by the MAP2K Pek1, which is regulated by the MAP3K Mkh1. MAPKs are important mediators of cellular responses to extracellular signals. The MPK1 subfamily is part of a larger superfamily that includes the catalytic domains of other STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.
Statistics
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PSSM-Id: 173750
View PSSM: cd07857
Aligned: 8 rows
Threshold Bit Score: 587.449
Threshold Setting Gi: 1173459
Created: 24-Sep-2008
Updated: 2-Mar-2014
Structure
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Aligned Rows:
 
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              #### ## #              # #                #              #               
gi 2499592   20 NFKVVKELGQGAYGIVCAARNVaskdqEAVAIKKITNVFSKSILTKRALREIKLLIHFRNHRNITCIYDLDIinp----y 95
gi 730747    22 RFQLIKEIGHGAYGIVCSARFAeaaedTTVAIKKVTNVFSKTLLCKRSLRELKLLRHFRGHKNITCLYDMDIvfyp--dg 99
gi 4193375   20 GYEVIKGLGKGSYGVVCSAKNIevednNKVAIKKITNIFSKKMLAKRALREIMLLQHFRNHKNITTLYDMDIvdp----s 95
gi 547783    22 HFHLTGKIGRGSHSLICSSTYTesneeTHVAIRKIPNAFGNKLSCKRTLRELKLLRHLRGHPNIVWLFDTDIvfyp--ng 99
gi 58271510 201 RWKLLRPLGQGAYGLVIQVQDAet--eIPIAVKCVTRVFDKVILARRALREITLLRHFGEHDNLTGLVDMDNvw-----d 273
gi 4580577   24 RYTVTKELGQGAYGIVCAATNVqt--gEGVAIKKVTNVFSKKILAKRALREIKLLQHFRGHRNITCLYDMDIprp----d 97
gi 28974309  21 PWQFVKELGQGAYGCVSSARNSst--gETCAVKKVTNVFQKKILTKRCLRELRLLHHFRGHKNITCLYDMDIvfdppgsg 98
gi 1173459   37 YYEIIQFLGKGAYGTVCSVKFKgrspaARIAVKKISNIFNKEILLKRAIRELKFMNFFKGHKNIVNLIDLEIvts----s 112
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               ####  # ##                                   # # ## #         ##  #     
gi 2499592   96 nFNEVYIYEELMEADLNAIIKSGQPLTDAHFQSFIYQILCGLKYIHSANVIHRDLKPGNLLVNADCELKICDFGLARGCS 175
gi 730747   100 sINGLYLYEELMECDMHQIIKSGQPLTDAHYQSFTYQILCGLKYIHSADVLHRDLKPGNLLVNADCQLKICDFGLARGYS 179
gi 4193375   96 kFNELYLYEELMQANLNSIIRSDQPLTDAHFQSFIYQILCGLKYIHSANVLHRDLKPSNLLINADCKLKICDFGLSRGIS 175
gi 547783   100 aLNGVYLYEELMECDLSQIIRSEQRLEDAHFQSFIYQILCALKYIHSANVLHCDLKPKNLLVNSDCQLKICNFGLSCSYS 179
gi 58271510 274 gYNEIYLYMEPMEADLHQIIRSGQPLGNAHIQFFIYQLLRGMKYIHSANVIHRDLKPGNLLVNSGCELKICDFGLARGFN 353
gi 4580577   98 nFNETYLYEELMECDLAAIIRSGQPLTDAHYQSFIYQILCGLKYIHSANVLHRDLKPGNLLVNADCELKICDFGLARGFS 177
gi 28974309  99 qFREVYLYEELMEADLHAIIRSGQPLSDAHFQSFLYQTLCGLKYIHSANVLHRDLKPGNLLVNADCELKICDFGLARGFQ 178
gi 1173459  113 pYDGLYCYQELIDYDLAKVIHSSVQLSEFHIKYFLYQILCGLKYIHSADVIHRDLKPGNILCTLNGCLKICDFGLARGIH 192
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                     # #### #                                     #                    
gi 2499592  176 ENPEE----NPGFMTEYVATRWYRAPEIMLSfssYHKGIDVWSVGCILAELLGGTPLFKGKDFVHQLNLILHQLGTPDEE 251
gi 730747   180 ENPVE----NSQFLTEYVATRWYRAPEIMLSyqgYTKAIDVWSAGCILAEFLGGKPIFKGKDYVNQLNQILQVLGTPPDE 255
gi 4193375  176 VNQGQ----GTEYMTEYVTTRWYRAPEVMLSfqsYSKAIDLWSVGCILAELLGRKPFFKGSNYVDQLNQIFCILGTPNEN 251
gi 547783   180 ENHKV----NDGFIKGYITSIWYKAPEILLNyqeCTKAVDIWSTGCILAELLGRKPMFEGKDYVDHLNHILQILGTPPEE 255
gi 58271510 354 PVKGEepqgEEGKLTEYVATRWYRAPEIMLSnrrYTTAIDVWSIGCILAELLGLKPVFKGKDYIEQMTLILETLGTPDEE 433
gi 4580577  178 IDPEE----NAGYMTEYVATRWYRAPEIMLSfqsYTKAIDVWSVGCILAELLGGRPFFKGRDYVDQLNQILHYLGTPNEE 253
gi 28974309 179 PGAVQtdqgQAGFMTEYVATRWYRAPEIMLSfanYTSSIDMWSVGCILAELLGGKPIFKGEDYVDQLNKILNLLGTPTED 258
gi 1173459  193 AGFFKchstVQPHITNYVATRWYRAPELLLSnqpYSKSVDIWAVGCILAEFYARKPVFMGRDSMHQIFEIIKVLGTPDKD 272
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
gi 2499592  252 TLSHISSSRAQEYVRSLPKQRPIPFETNFPKA--NPLALDLLAKLLAFDPNRRISVDDALEHPYLAVWHDPSDEPv-cDS 328
gi 730747   256 TLRRIGSKNVQDYIHQLGFIPKVPFVNLYPNA--NSQALDLLEQMLAFDPQKRITVDEALEHPYLSIWHDPADEPv-cSE 332
gi 4193375  252 IISKIKSASAQSYIRSLPTLPKMPYSKIFPYA--NPDALDLLNCLLTFDPYDRISCEEALEHPYLIIWHDPNKEPv-cSE 328
gi 547783   256 TLQEIASQKVYNYIFQFGNIPGRSFESILPGA--NPEALELLKKMLEFDPKKRITVEDALEHPYLSMWHDIDEEFs-cQK 332
gi 58271510 434 TMARVASEKALTFLKTLPTYEKKDLGSIFPDA--DPLAVDLTDKLLEFDPTRRIDVPSALTHAYVERYHDPEDEPs-cDK 510
gi 4580577  254 TLSRIGSPRAQEYVRNLPFMPKVPFQRLFPNA--NPDALDLLDRMLAFDPSSRISVEEALEHPYLAIWHDASDEPs-cPT 330
gi 28974309 259 TLRRVGSPRAQDYIRSLPIKPRVKFGTLYPNA--SPLALDLLSKLLTFDPAKRYGCEEALEHPYLAVWHDPADEPl-cEV 335
gi 1173459  273 ILIKFGTIKAWNLGKNSNNPVYKKIPWSNIFPfaSHEAINLIESLLHWDSTHRLNVEQAISHPFLNEVRKPDDEPvclQG 352
                       330       340
                ....*....|....*....|....*
Feature 1                                
gi 2499592  329 VFDFSFEyi--eDANELRRVILDEV 351
gi 730747   333 KFEFSFEsv--nDMEDLKQMVIQEV 355
gi 4193375  329 QFDFGFEai--dSIEEIRQMIINEV 351
gi 547783   333 TFRFEFEhi--eSMAELGNEVIKEV 355
gi 58271510 511 IFDKWEEveslsTIEELKEAITREI 535
gi 4580577  331 TFDFHFEvv--dDVQEMRRMIYEEV 353
gi 28974309 336 PFDFSFEee--dSVSGMRDLILEEV 358
gi 1173459  353 PFDFTYEse-lnSMSKLRDYLVEEV 376

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