2VWI


Conserved Protein Domain Family
STKc_OSR1_SPAK

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cd06610: STKc_OSR1_SPAK (this model, PSSM-Id:173726 is obsolete and has been replaced by 270787)
Click on image for an interactive view with Cn3D
Catalytic domain of the Protein Serine/Threonine Kinases, Oxidative stress response kinase and Ste20-related proline alanine-rich kinase
Serine/threonine kinases (STKs), oxidative stress response kinase (OSR1) and Ste20-related proline alanine-rich kinase (SPAK) subfamily, catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The OSR1 and SPAK subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. SPAK is also referred to as STK39 or PASK (proline-alanine-rich STE20-related kinase). OSR1 and SPAK regulate the activity of cation-chloride cotransporters through direct interaction and phosphorylation. They are also implicated in cytoskeletal rearrangement, cell differentiation, transformation and proliferation. OSR1 and SPAK contain a conserved C-terminal (CCT) domain, which recognizes a unique motif ([RK]FX[VI]) present in their activating kinases (WNK1/WNK4) and their substrates.
Statistics
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PSSM-Id: 173726
Aligned: 15 rows
Threshold Bit Score: 448.344
Threshold Setting Gi: 15233460
Created: 2-Aug-2006
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 31 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:The active site is composed of the ATP binding site and the substrate binding site. The substrate binding site is based on the binding of human PAK4 to a consensus peptide.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                #####   #             # #                              #               #
2VWI_C        15 DDYELQEVIGSGATAVVQAAYCap-kkEKVAIKRINLEKCqt-smDELLKEIQAMSQChHPNIVSYYTSFVVkdeLWLVM 92  human
AAC72238      63 DAYELQEVIGSGATAVVQAALCkp-rqERVAIKRINLEKCqt-smDELLKEIQAMSQCsHPNVVTYYTSFVVkdeLWLVM 140 human
XP_418527     15 DDYELQEVIGSGATAVVQAAFCvp-kkEKVAIKRINLEKCqt-smDELLKEIQAMSQChHPNIVSYYTSFVVkdeLWLVM 92  chicken
AAH77793      19 DDYELQEVIGSGATAVVQAAFCap-kkEKVAIKRINLEKCqt-smDELLKEIQAMSQChHPNIVSYYTSFVVkdeLWLVM 96  African clawed ...
AAH67372      15 DDYELQEVIGSGATAVVQAAYCvp-rkEKVAIKRINLEKCqt-smDELLKEIQAMSQChHPNIVSYYTSFVVkdeLWLVM 92  zebrafish
2262107       31 KDYKLMEEIGHGASAVVYRAIYlp-tnEVVAIKCLDLDRCns-nlDDIRRESQTMSLIdHPNVIKSFCSFSVdhsLWVVM 108 thale cress
BAD28003      31 KDYELLEPVGDGATAVVRRARClplggEVVAVKIMNMSQRseddvNHASEEVKMMSTIdHDNLLGAYCSFTEgetLWIIM 110 Japanese rice
NP_193184     13 EAYEIICKIGVGVSASVYKAICipmnsMVVAIKAIDLDQSra-dfDSLRRETKTMSLLsHPNILNAYCSFTVdrcLWVVM 91  thale cress
XP_001310230   8 SQYKILNQVGKGVTAVVYVAECla-ngRTVALKTIDLETCpi-eiENLRHEVAFWSTSqSPNVVKYHGSFVEgsvLYIVM 85  Trichomonas vag...
XP_001315036   9 SHYKFIEPIGRGSTSEVLSAICld-nqNEISIKKIDMEKYpi-elEFLRQEVSFWSSSaHPNVVGYYGSFVEgtvIYILM 86  Trichomonas vag...
Feature 1        ###  ##  #                                           # #### #          #  #     
2VWI_C        93 KLLsgGSVLDIIKHIvakgehksgVLDESTIATILREVLEGLEYLHKNGQIHRDVKAGNILLGedGSVQIADFGVSAFLA 172 human
AAC72238     141 KLLsgGSMLDIIKYIvnrgehkngVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGedGSVQIADFGVSAFLA 220 human
XP_418527     93 KLLsgGSVLDIIKHIvakgehktgVLDEPCIATILKEVLEGLDYLHKNGQIHRDVKAGNILLGedGSVQIADFGVSAFLA 172 chicken
AAH77793      97 KLMsgGSVLDIIKHIiargehkngVLDEPSIATMLKEVLEGLEYLHKNGQIHRDVKAGNILLGddGSVQIADFGVSAFLA 176 African clawed ...
AAH67372      93 KLLsgGSVLDVIKHIiskgehktgVLDEPSIATVLKEVLQGLEYLHKNGQIHRDLKAGNILLGedGSVQIADFGVSAFLA 172 zebrafish
2262107      109 PFMaqGSCLHLMKTAys------dGFEESAICCVLKETLKALDYLHRQGHIHRDVKAGNILLDdnGEIKLGDFGVSACLF 182 thale cress
BAD28003     111 PYMagGSCFHLMKSSyp------kGFDEKFIAFVLRETLEGLAYLHRYALVHRDVKAGNILLDqhKGVKLADFGASASLY 184 Japanese rice
NP_193184     92 PFMscGSLHSIVSSSfp------sGLPENCISVFLKETLNAISYLHDQGHLHRDIKAGNILVDsdGSVKLADFGVSASIY 165 thale cress
XP_001310230  86 EYMsaGSCYEIMRYSnk-----kgIQDEAIIAAILHEVLQALCYFHDNRQIHRDIKAGNILINehGDVKIGDFGISANLL 160 Trichomonas vag...
XP_001315036  87 EYCsaGSIYDIMRYGfp-----rgFNDEFIIATILKGVLNALNYIHSNGQIHRDIKPGNILLCadGSVKIGDFGVAASLF 161 Trichomonas vag...
Feature 1                          #### #                           #        #                   
2VWI_C       173 TGGDitr-----nkvRKTFVGTPCWMAPEVMEQvrGYDFKADIWSFGITAIELAtGAAPYHKyPPMKVLMLTLQN--DPP 245 human
AAC72238     221 TGGDvtr-----nkvRKTFVGTPCWMAPEVMEQvrGYDFKADMWSFGITAIELAtGAAPYHKyPPMKVLMLTLQN--DPP 293 human
XP_418527    173 TGGDitr-----nkvRKTFVGTPCWMAPEVMEQvrGYDFKADIWSFGITAIELAtGAAPYHKyPPMKVLMLTLQN--DPP 245 chicken
AAH77793     177 TGGDitr-----nkvRKTFVGTPCWMAPEVMEQvrGYDFKADIWSFGITAIELAtGAAPYHKyPPMKVLMLTLQN--DPP 249 African clawed ...
AAH67372     173 TGGDmtr-----nkvRKTFVGTPCWMAPEVMEQvkGYDFKADIWSFGITAIELAtGAAPYHKyPPMKVLMLTLQN--DPP 245 zebrafish
2262107      183 DNGDrq-------raRNTFVGTPCWMAPEVLQPgnGYNSKADIWSFGITALELAhGHAPFSKyPPMKVLLMTIQN--APP 253 thale cress
BAD28003     185 DPMInrh------gkRKTLVGTPCWMAPEVMEQk-EYDAKADIWSFGITALELAhGHAPFSTqPPAKVFLLTLQH--APP 255 Japanese rice
NP_193184    166 EPVTsssgttssslrLTDIAGTPYWMAPEVVHShtGYGFKADIWSFGITALELAhGRPPLSHlPPLKSLLMKITKrfHFS 245 thale cress
XP_001310230 161 EGGQrk-------raRFTVIGTPCYMAPEVLKEeeGYTEKADIWSLGITAIELAiGAAPYANlFPLEVIVKIVNS--PPP 231 Trichomonas vag...
XP_001315036 162 QDGQrq-------raRYTVIGTPCYMAPEVLTEdhGYTEKADIWSLGITAIELAtGEAPYSKlKPMEIMVKILKS--PPS 232 Trichomonas vag...
Feature 1                                                        
2VWI_C       246 SLETGvqdk--emlkKYGKSFRKMISLCLQKdPEKRPTAAELLRHKFF 291 human
AAC72238     294 TLETGvedk--emmkKYGKSFRKLLSLCLQKdPSKRPTAAELLKCKFF 339 human
XP_418527    246 SLETGvqdk--emlkKYGKSFRKMISSCLQKdPEKRPTAAELLRHKFF 291 chicken
AAH77793     250 TLETGvqdk--emlkKYGKSFRKMISSCLQKdPEKRPTAAELLKHKFF 295 African clawed frog
AAH67372     246 CLETGitdk--emvkKYGKSLRKMISQCLQKePEKRPTSSELLKHKFF 291 zebrafish
2262107      254 GLDYDrd-------kKFSKSFKEMVAMCLVKdQTKRPTAEKLLKHSCF 294 thale cress
BAD28003     256 SLHNTkd-------kKFSKSFKQMIATCLMKdPSKRPTAQHLLELPFF 296 Japanese rice
NP_193184    246 DYEINtsgsskkgnkKFSKAFREMVGLCLEQdPTKRPSAEKLLKHPFF 293 thale cress
XP_001310230 232 QLPEDa---------KFSSAFRDFIKSCLVQsPAKRPTAHQLLEHKFF 270 Trichomonas vaginalis G3
XP_001315036 233 KLPTNa---------PYSAEFRNFVEKCLQSdPMNRATAEELLRHPFI 271 Trichomonas vaginalis G3

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