Conserved Protein Domain Family
STKc_cPKC_alpha

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cd05615: STKc_cPKC_alpha (this model, PSSM-Id:173706 is obsolete and has been replaced by 270766)
Catalytic domain of the Protein Serine/Threonine Kinase, Classical Protein Kinase C alpha
Serine/Threonine Kinases (STKs), Classical Protein Kinase C (cPKC) subfamily, alpha isoform, catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The cPKC subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. PKCs are classified into three groups (classical, atypical, and novel) depending on their mode of activation and the structural characteristics of their regulatory domain. PKCs undergo three phosphorylations in order to take mature forms. In addition, cPKCs depend on calcium, DAG (1,2-diacylglycerol), and in most cases, phosphatidylserine (PS) for activation. There are four cPKC isoforms, named alpha, betaI, betaII, and gamma. PKC-alpha is expressed in many tissues and is associated with cell proliferation, apoptosis, and cell motility. It plays a role in the signaling of the growth factors PDGF, VEGF, EGF, and FGF. Abnormal levels of PKC-alpha have been detected in many transformed cell lines and several human tumors. In addition, PKC-alpha is required for HER2 dependent breast cancer invasion.
Statistics
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PSSM-Id: 173706
View PSSM: cd05615
Aligned: 6 rows
Threshold Bit Score: 703.679
Threshold Setting Gi: 227490
Created: 31-Aug-2007
Updated: 17-Jan-2013
Structure
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Aligned Rows:
  next features
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the binding of peptide substrates and ATP analogs to the AGC kinases, PKA and PKB.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              #####   #            # #                                #               #
gi 125550   338 DFNFLMVLGKGSFGKVMLADRkgteELYAIKILKKDvviqdDDVECTMVEKRVLALLdkPPFLTQLHSCFQTvdRLYFVM 417
gi 50604098 343 DFCFLMVLGKGSFGKVMLAERkgsdELYAIKILKKDvviqdDDVECTMVEKRVLALQekPPFLTQLHSCFQTvdRLYFVM 422
gi 61098322 340 DFNFLMVLGKGSFGKVMLADRknteELYAIKILKKDvviqdDDVECTMVEKRVLALQdkPPFLTQLHSCFQTvdRLYFVM 419
gi 227490   342 DFSFLMVLGKGSFGKVMVGRRkgsdELYAVKILKKDvviqdDDVECTMIEKRVLALQdkPPFLTQLQSCFQTvdRLYFVM 421
gi 22023043 336 DFNFLALLGKGSFGKVMLAEMkstdELYAIKILKKDvviqdDDVECTMVEKRVLAQRdkPPFLTQLHSCFQTvdRLYFVM 415
gi 47223946 314 TDGPSSLMPSNIMDQVMLVEMkatgELYAVKILKKDvviqdDDVECTMVEKRVLALQdkPPFLTQLHSCFQTvdRLYFVM 393
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1       # #   # #                                    # # ## #         ##  #             
gi 125550   418 EYVnGGDLMYHIQQvgkFKEPQAVFYAAEISIGLFFLHkRGIIYRDLKLNNVMLNseGHIKIADFGMCKEHMMDGVTTRT 497
gi 50604098 423 EYVsGGDLMYHIQQvgkFKEPQAVFYAAEISVGLFFLHrKGICYRDLKLDNVMLDfeGHIKIADFGMCKEHMVEGVTTRT 502
gi 61098322 420 EYVnGGDLMYHIQQvgkFKEPQAVFYAAEISVGLFFLHnRGIVYRDLKLDNVMLDseGHIKIADFGMCKEHMLDGVTTRT 499
gi 227490   422 EYVsGGDLMYHIQQvgkFKEPQAVFYAAEISVGLFFLHkKGIVYRDLKLDNVMLDseGHIKIADFGMCKEHVVEGVTTRT 501
gi 22023043 416 EYInGGDLMYHIQRmgkFKEPQAVFYAAEIAVGLFFLHrKGIIYRDLKLDNVMLDseGHIKIADFGMCKESMYEGMTTRT 495
gi 47223946 394 EYVnGGDLMYHIQQvgkFKEPQAVFYAAEIAVGLFFLHeRGIIYRDLKLDNVMLDseGHIKIADFGMCKENMVDGVCTRT 473
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1       ######                          #     #  #                                      
gi 125550   498 FCGTPDYIAPEIIAYqpyGKSVDWWAYGVLLYEMLAGQPPFDGEdeDELFQSIMEHNVSYPKSlsKEAVSICKGLMTkqP 577
gi 50604098 503 FCGTPDYIAPEIIAYqpyGRSVDWWAYGVLLYEMLAGQPPFDGEdeDELFQSIMEHNVSYPKSmsKEAVSICKGLMTkhP 582
gi 61098322 500 FCGTPDYIAPEIIAYqpyGKSVDWWAYGVLLYEMLAGQPPFDGEdeDELFQSIMEHNVSYPKSlsKEAVSICKGLMTkhP 579
gi 227490   502 FCGTPDYIAPEIIAYqpyGRSVDWWAIGVLLYEMLAGQPPFDGEdeDELFQSIMEHNVAYPKSmsKEAVSICKPLMTkhP 581
gi 22023043 496 FCGTPDYIAPEIIAYqpyGKSVDWWAYGVLLYEMLAGQPPFDGEdeDELFQSIMEHNVSYPKSmsKEAVSICKGLMTkhP 575
gi 47223946 474 FCGTPDYIAPEIIAYqpyGLSVDWWAYGVLLYEMLAGQPPFDGEdeDELFQSIMEHNVSYPKSlsKEAVSICKGLMAklP 553
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
gi 125550   578 AKRLGCgpegERDVREHAFFRRIDWEKLENREIQPPFKPKVCGKgaENFDKFFTRGQPVLTPPDqlviaNIDQSDFEGFS 657
gi 50604098 583 VKRLGCgpegERDIREHTFFRRIDWDKLENREIQPPFKPKVCGKgaENFDKFFTRGQPVLTPPDqlviaNIDQADFEGFS 662
gi 61098322 580 AKRLGCglegERDIREHAFFRRIDWEKLENREIQPPFKPKVCGKgaENFDKFFTRGQPVLTPPDqlviaNIDQSDFEGFS 659
gi 227490   582 VKRLGCgpegERDVREHTFFRRIDWDKLENREIQPPFKPKVCGKgaENFDKFFTRGQPVLTPPDqlviaNIDQADFEGFT 661
gi 22023043 576 SKRLGCgpegEMDIREQAFFRRIDWDRLANREIQPPFKPKVGGKgaENFDKFFTRTQPVLTPPDhlviaNIDQSEFAGFS 655
gi 47223946 554 SKRLGCgqegERDIRDHVFFRRIDWKRLENREIQPPFKPKVCGKaaENFDKFFTRTQPQLTPPDelviaNIDQAEFANFS 633

                ...
Feature 1          
gi 125550   658 YVN 660
gi 50604098 663 YIN 665
gi 61098322 660 YVN 662
gi 227490   662 YVN 664
gi 22023043 656 YVN 658
gi 47223946 634 FIN 636

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