1VZO


Conserved Protein Domain Family
STKc_MSK1_N

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cd05613: STKc_MSK1_N (this model, PSSM-Id:173704 is obsolete and has been replaced by 270764)
Click on image for an interactive view with Cn3D
N-terminal catalytic domain of the Protein Serine/Threonine Kinase, Mitogen and stress-activated kinase 1
Serine/Threonine Kinases (STKs), Mitogen and stress-activated kinase (MSK) subfamily, MSK1, N-terminal catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The MSK subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. MSKs contain an N-terminal kinase domain (NTD) from the AGC family and a C-terminal kinase domain (CTD) from the CAMK family, similar to 90 kDa ribosomal protein S6 kinases (RSKs). MSKs are activated by two major signaling cascades, the Ras-MAPK and p38 stress kinase pathways, which trigger phosphorylation in the activation loop (A-loop) of the CTD of MSK. The active CTD phosphorylates the hydrophobic motif (HM) of NTD, which facilitates the phosphorylation of the A-loop and activates the NTD, which in turn phosphorylates downstream targets. MSK1 plays a role in the regulation of translational control and transcriptional activation. It phosphorylates the transcription factors, CREB and NFkappaB. It also phosphorylates the nucleosomal proteins H3 and HMG-14. Increased phosphorylation of MEK1 is associated with the development of cerebral ischemic/hypoxic preconditioning.
Statistics
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PSSM-Id: 173704
View PSSM: cd05613
Aligned: 3 rows
Threshold Bit Score: 606.993
Threshold Setting Gi: 189531032
Created: 8-Nov-2007
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 31 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the binding of peptide substrates and ATP analogs to the AGC kinases, PKA and PKB.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               #####   #               # #                                 #            
1VZO_A        55 NFELLKVLGTGAYGKVFLVRKIsghdtgKLYAMKVLKKATIVQKAKttEHTRTERQVLEHIrqSPFLVTLHYAFQTetKL 134
gi 82231192   38 NFELLKVLGTGAYGKVFLVRKVsghdagKLYAMKVLKKATIVQKAKttEHTRTERQVLEHIrqSPFLVTLHYAFQTdtKL 117
gi 189531032  33 NFELLKVLGTGAYGKVFLVRKVsghdsgKLYAMKVLKKATIVQKAKtaEHTRTERQVLEHIrqSPFLVTLHYAFQTdtKL 112
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           ## #   # #                                                 # # ## #         #
1VZO_A       135 HLILDYInGGELFTHLSQrerFTEHEVQIYVGEIVLALEHLHK-------------LGIIYRDIKLENILLDsnGHVVLT 201
gi 82231192  118 HLILDYInGGELFTHLSQrerFSENEVQIYIGEIVLALEHLHK-------------LGIIYRDIKLENILLDsdGHVVLT 184
gi 189531032 113 HLILDYVnGGELFTHLVQrvrFKEQEVTLYSGEIVLALEHLHKlihfcfflflffqLGIVYRDLKLENILLDsnGHIVLT 192
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        #  #              ######                            #     #  #                  
1VZO_A       202 DFGLSKEFVAdeTERAYDFCGTIEYMAPDIVRGgdsghDKAVDWWSLGVLMYELLTGASPFTVDgeknsQAEISRRILKS 281
gi 82231192  185 DFGLSKEFLTdeNERAYSFCGTIEYMAPDIVRGgdtghDKAVDWWSVGVLMYELLTGASPFTVDgeknsQAEISRRILKS 264
gi 189531032 193 DFGLSKEFHE--VERAYSICGTIEYMAPEIVAGgegghDKAVDWWSMGVLMYELLTGGSPFTVDgdensHSDIAERIMKK 270
                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
Feature 1                                                                       
1VZO_A       282 EPPYPQEmsALAKDLIQRLLMKdPKKRLGCgprdADEIKEHLFFQKINWDDLAAKKVPAPFKP 344
gi 82231192  265 EPPYPQEmsALSKDIIQRLLMKdPKKRLGCgptdADEIKQHPFFQNINWDDLAAKKVPAPFKP 327
gi 189531032 271 DPPFPKDmgPLAKDIIQRLLIKdPKKRLGSgpsgAQNVKKHPFYQKLNWEDLAAKKVRAPFKP 333

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