Conserved Protein Domain Family
STKc_nPKC_epsilon

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cd05591: STKc_nPKC_epsilon (this model, PSSM-Id:173682 is obsolete and has been replaced by 270743)
Catalytic domain of the Protein Serine/Threonine Kinase, Novel Protein Kinase C epsilon
Serine/Threonine Kinases (STKs), Novel Protein Kinase C (nPKC), epsilon isoform, catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The nPKC subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. PKCs are classified into three groups (classical, atypical, and novel) depending on their mode of activation and the structural characteristics of their regulatory domain. nPKCs are calcium-independent, but require DAG (1,2-diacylglycerol) and phosphatidylserine (PS) for activity. There are four nPKC isoforms, delta, epsilon, eta, and theta. PKC-epsilon has been shown to behave as an oncoprotein. Its overexpression contributes to neoplastic transformation depending on the cell type. It contributes to oncogenesis by inducing disordered cell growth and inhibiting cell death. It also plays a role in tumor invasion and metastasis. PKC-epsilon has also been found to confer cardioprotection against ischemia and reperfusion-mediated damage. Other cellular functions include the regulation of gene expression, cell adhesion, and cell motility.
Statistics
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PSSM-Id: 173682
View PSSM: cd05591
Aligned: 9 rows
Threshold Bit Score: 649.216
Threshold Setting Gi: 156368406
Created: 4-Apr-2007
Updated: 17-Jan-2013
Structure
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Aligned Rows:
  next features
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the binding of peptide substrates and ATP analogs to the AGC kinases, PKA and PKB.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          #####   #            # #                                #               ## #  
gi 80474928  412 KVLGKGSFGKVMLAELkgkdEVYAVKVLKKDvilqdDDVDCTMTEKRILALArkHPYLTQLYCCFQTkdRLFFVMEYVnG 491
gi 125547    307 KVLGKGSFGKVMLAEKkgtdEIYAIKVLKKDaiiqdDDVDCTMTEKRILALAanHPFLTALHSCFQTpdRLFFVMEYVnG 386
gi 22085162  419 KVLGKGSFGKVMLGERkgtdEVYAIKVLRKDvilqdDDVDCTMTEKRILTLAakHPFLTALCCSFQTedRLFFVMEYVnG 498
gi 6016444   407 KVLGKGSFGKVMLAEKkgtdEVYAIKVLKKDviiqdDDVECTMTEKRILALSakHPFLTALHSSFQTkeRLFFVMEYVnG 486
gi 47224750  432 KVLGKGSFGKVMLAELkgsdEVYAVKVLKKDvilqdDDVDCTLTEKRILALArkHPYLTQLFCCFQTkdRLFFVMEYVnG 511
gi 118087961 418 KVLGKGSFGKVMLAELkgkdEVYAVKVLKKDvilqdDDVDCTMTEKRILALArkHPYLTQLYCCFQTkdRLFFVMEYVnG 497
gi 17562588  382 KVLGKGSFGKVMLAERkgtdEVYAIKILKKDvivqdDDVECTMCEKRILSLAakHPFLTALHSSFQTsdRLFFVMEYVnG 461
gi 115529015 417 KVLGKGSFGKVMLAELkgrdEVYAVKVLKKDvilqdDDVDCTMTEKRILALArrHPYLTQLYCCFQTkdRLFFVMEYVnG 496
gi 156368406 387 KVLGKGSFGKVLLAERkgtdEVYAIKILKKEsvlqdDDVECTMIERRVLALSagHPFLTSLHSSFQTveRLFFVMEYVnG 466
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         # #                                    # # ## #         ##  #             #####
gi 80474928  492 GDLMFQIQRsrkFDEPRSRFYAAEVTSALMFLHQHGVIYRDLKLDNILLDaeGHCKLADFGMCKEGILNGVTTTTFCGTP 571
gi 125547    387 GDLMFQIQKarrFEASRAAFYAAEVTLALQFLHTHGVIYRDLKLDNILLDqeGHCKLADFGMCKEGIMNGMLTTTFCGTP 466
gi 22085162  499 GDLMFQIQKarkFDEARARFYAAEVTLALMFLHRHGVIYRDLKLDNILLDseGHCKIADFGMCKEGIAGGATTTTFCGTP 578
gi 6016444   487 GDLMFQIQRarkFDEPRARFYAAEVTLALMFLHRHGIIYRDLKLDNILLDaeGHCKIADFGMCKEGMTENKLTQTFCGTP 566
gi 47224750  512 GDLMFQIQRsrkFDEARSRFYAAEVTSALMFLHRHGVIYRDLKLDNILLDadGHCKLADFGMCKEGILNGITTTTFCGTP 591
gi 118087961 498 GDLMFQIQRsrkFDEPRSRFYAAEVTSALMFLHQHGVIYRYLDSGNILLDaeGHCKLAAFGMCKEGILNGVTTTTFCGTP 577
gi 17562588  462 GDLMFQIQRarkFDESRARFYAAEVTCALQFLHRNDVIYRDLKLDNILLDaeGHCRLADFGMCKEGINKDNLTSTFCGTP 541
gi 115529015 497 GDLMFQIQRsrkFDEPRSRFYAAEVTSALMFLHQHGVIYRDLKLDNILLDaeGHCKLADFGMCKEGILNGVTTTTFCGTP 576
gi 156368406 467 GDLMFQIQKsrkFDENRSRFYAAEVVLALQFLHQNGVIYRDLKLDNVLLDadGHIKLADFGMCKENMAPGKTTTTFCGTP 546
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        #                          #     #  #                                           
gi 80474928  572 DYIAPEILQEleyGPSVDWWALGVLMYEMMAGQPPFEADneDDLFESILHDDVLYPVWlsKEAVSILKAFMTknPHKRLG 651
gi 125547    467 DYIAPEILKEqeyGASVDWWALGVLMYEMMAGQPPFEADneDELFDSIMHDDVLYPVWlsREAVSILKGFLTknPEQRLG 546
gi 22085162  579 DYIAPEILQEleyGPSVDWWALGVLMYEMMAGQPPFEADneDDLFESILHEDVVYPVWlsKEAVSVLKGFMTknPAKRLG 658
gi 6016444   567 DYIAPEILQElkyDASVDWWALGVLMYEMMAGQPPFEADneDDLFESILHDDVLYPVWlsKEAVSILKGFMTknPAKRLG 646
gi 47224750  592 DYIAPEILQEldyGPSVDWWALGVLMYEMMAGQPPFEADneDDLFESILHDDVLYPVWlsKEAVSILKAFMTksPNKRLG 671
gi 118087961 578 DYIAPEILQEleyGPSVDWWALGVLMYEMMAGQPPFEADneDDLFESILHDDVLYPVWlsKEAVSILKAFMTknPNKRLG 657
gi 17562588  542 DYIAPEILQEmeyGVSVDWWALGVLMYEMMAGQPPFEADneDDLFEAILNDDVLYPVWlsKEAVNILKAFMTknAGKRLG 621
gi 115529015 577 DYIAPEILQEleyGPSVDWWALGVLMYEMMAGQPPFEADneDDLFESILHDDVLYPVWlsKEAVSILKAFMTknPNKRLG 656
gi 156368406 547 DYIAPEILQEqpyGFSVDWWALGVLMYEMMAGQPPFEAEneEDLFESILHDDVLYPVWlsREAVSILKGFMTknYLKRLG 626
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 80474928  652 CvasqngeDAIKQHPFF-KEIDWVLLEqKKIKPPFKPRIKTKRDVNNFDQDFTREEPVLTLvdeaivkqINQEEFKGFSY 730
gi 125547    547 Ctg---deNEIRKHPFF-AKLDWKELEkRNIKPPFRPKMKNPRDANNFDAEFTKEDPVLTPignevvrcINQDEFAGFSF 622
gi 22085162  659 CvpsqgleQAILNHIFF-KEIDWDALEsRKVKPPFRPKIKTKRDVNNFDQDFTKEDPVLTQvnpdvirtINQEEFRGFSV 737
gi 6016444   647 CvttqgceKAILVHPFFhEKIDWEALEqRKVKPPFKPKIKNKTDANNFDRDFTSEDPVLTPvdpaviktINQEEFRGFSF 726
gi 47224750  672 CvvaqgleEAIKLHPFF-REIDWTLLEqRKIRPPFKPRIKTKRDVNNFDQDFTREEPVLTPvdnsiikqINQDEFKGFSY 750
gi 118087961 658 CvasqngeDAIKQHPFF-KEIDWVLLEqRKIKPPFKPRIKTKRDVNNFDQDFTREEPVLTLvdetiikqINQEEFKGFSY 736
gi 17562588  622 CvvsqggeDAIRAHPFF-REIDWDALEsRQVKPPFKPKIKSKRDANNFDSDFTKEEPVLTPsdpavvraINQDEFRGFSF 700
gi 115529015 657 CvaaqgleEAIKQHPFF-KEIDWVLLEqRKVKPPFKPRIKTKRDVNNFDQDFTREEPVLTLvdeaivkqINQEEFKGFSY 735
gi 156368406 627 Ctpt-steSSIREHAFF-RLINFEKLEaRQINPPFKPKIRSRTDVGNFDTDFTKEEPKLTPtdrnvivnIDQREFRGFSF 704

                 ..
Feature 1          
gi 80474928  731 FG 732
gi 125547    623 VN 624
gi 22085162  738 VN 739
gi 6016444   727 AN 728
gi 47224750  751 FG 752
gi 118087961 737 FG 738
gi 17562588  701 IN 702
gi 115529015 736 FG 737
gi 156368406 705 VN 706

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