1VZO


Conserved Protein Domain Family
STKc_MSK_N

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cd05583: STKc_MSK_N (this model, PSSM-Id:173674 is obsolete and has been replaced by 270735)
Click on image for an interactive view with Cn3D
N-terminal catalytic domain of the Protein Serine/Threonine Kinase, Mitogen and stress-activated kinase
Serine/Threonine Kinases (STKs), Mitogen and stress-activated kinase (MSK) subfamily, N-terminal catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The MSK subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. MSKs contain an N-terminal kinase domain (NTD) from the AGC family and a C-terminal kinase domain (CTD) from the CAMK family, similar to 90 kDa ribosomal protein S6 kinases (RSKs). MSKs are activated by two major signaling cascades, the Ras-MAPK and p38 stress kinase pathways, in response to various stimuli such as growth factors, hormones, neurotransmitters, cellular stress, and pro-inflammatory cytokines. This triggers phosphorylation in the activation loop (A-loop) of the CTD of MSK. The active CTD phosphorylates the hydrophobic motif (HM) in the C-terminal extension of NTD, which facilitates the phosphorylation of the A-loop and activates the NTD, which in turn phosphorylates downstream targets. MSKs are predominantly nuclear proteins. They are widely expressed in many tissues including heart, brain, lung, liver, kidney, and pancreas. There are two isoforms of MSK, called MSK1 and MSK2.
Statistics
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PSSM-Id: 173674
View PSSM: cd05583
Aligned: 6 rows
Threshold Bit Score: 547.081
Threshold Setting Gi: 115532262
Created: 23-Aug-2006
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 31 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the binding of peptide substrates and ATP analogs to the AGC kinases, PKA and PKB.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                #####   #               # #                                 #            
1VZO_A         55 NFELLKVLGTGAYGKVFLVRKIsghdtgKLYAMKVLKKATIVQKAKttEHTRTERQVLEHIrqSPFLVTLHYAFQTetKL 134  human
Q9V3I5        260 DFKIIRVLGTGAYGRVFLVRKLtrhdagKLYAMKVLNKITVVQKRKtaEHTKTERVVLEAIqrNPFLVSLHYAFQSssKL 339  fruit fly
XP_786494       8 DTLMVRHELKHAYGKVFLVRKVgnhnqgKLYAMKVLKKATIVQKAKtaEHTMTERQVLEAVrsCPFLVTLHYAFQTdsKL 87   purple urchin
XP_001631226   15 NFELLRVLGTGAYGKVFLARKRgghhdgRLFAMKVLKKATIVQKAKtaEHTRTERQVLEAVrrCPFLVTLHWAFQTeaKL 94   starlet sea a...
NP_001040645   16 NFALLRVLGKGAYGKVFLVRKVggkdhnTIYAMKVLRKTRVLTKQKtlEHTMAERQVLERLrgTPFLVNLFYAFQTdtKL 95   nematode
AAC67395       16 NFELLKVLGTGAYGKVFLVRKAgghdagKLYAMKVLRKAALVQRAKtqEHTRTERSVLELVrqAPFLVTLHYAFQTdaKL 95   human
Feature 1            ## #   # #                                    # # ## #         ##  #         
1VZO_A        135 HLILDYInGGELFTHLSQrerFTEHEVQIYVGEIVLALEHLHKLGIIYRDIKLENILLDsnGHVVLTDFGLSKEFVAd-e 213  human
Q9V3I5        340 YLVLDFAnGGELFTHLYHsenFEESRVRVYIAEVVLALEQLHQLGIIYRDIKLENILLDgeGHIVLSDFGLSKILTAe-n 418  fruit fly
XP_786494      88 NLILDYVnGGELFTHLYQrehFRESEVRIYIAEIIIALDCLHQLGIIYRDIKLENILLDkdGHVVLTDFGLSKEFLTn-t 166  purple urchin
XP_001631226   95 HLIMDYVnGGELFTHLYQrekFTEDEVRLYIGEIVVALDHLHQLGIIYRDIKLENILLDkdGHVVLTDFGLSKEFAApns 174  starlet sea a...
NP_001040645   96 HIVMEYVrGGELFTHLCSrghFDLEAARFVIAELVVAIDSLHQRKVIYRDLKLENILLDeeGHVKLTDFGLSKLFLPg-e 174  nematode
AAC67395       96 HLILDYVsGGEMFTHLYQrqyFKEAEVRVYGGEIVLALEHLHKLGIIYRDLKLENVLLDseGHIVLTDFGLSKEFLTe-e 174  human
Feature 1               ######                            #     #  #                              
1VZO_A        214 TERAYDFCGTIEYMAPDIVRGgdsghDKAVDWWSLGVLMYELLTGASPFTVDgeknsQAEISRRILKSEPPYPQEmsALA 293  human
Q9V3I5        419 EYRAHSFCGTLEYMAPEIIRTgppghDSAVDWWSVGVLTFELLTGASPFATSdgqvqQSEISRRIQKEQPMIPSSfsANA 498  fruit fly
XP_786494     167 EDRAYSFCGTIEYMAPEVVRGgsdghNKAVDWWSLGVLTYELLTGASPFTVEgernsQSQISQRILTSHPPMPNTfsKEV 246  purple urchin
XP_001631226  175 GERAYSFCGTIEYMAPEVVKGgsrghDKAVDWWSLGVLMYELLTGASPFTVDgeknsQSEISKRILHNNPPMPHDlsAEA 254  starlet sea a...
NP_001040645  175 LDRANSYCGTIEYMSPEVINRpeggySDVVDWWSLGVISFELLTGCSPFTVDgaqnsSKDIAKRIMTKKVPFPKTmdVDA 254  nematode
AAC67395      175 KERTFSFCGTIEYMAPEIIRSkt-ghGKAVDWWSLGILLFELLTGASPFTLEgerntQAEVSRRILKCSPPFPPRigPVA 253  human
Feature 1                                                            
1VZO_A        294 KDLIQRLLMKdPKKRLGCgprdADEIKEHLFFQKINWDDLAAKKVPAPFKP 344  human
Q9V3I5        499 RDFVLKMLEKnPKRRLGGnhrdASEIKEHPFFNGINWQELRTKRRKAPYKP 549  fruit fly
XP_786494     247 KDFINKLLVKdPTKRLGCn--gVKDIKSHSFFKGLNWDDVAAKRVSPPFRP 295  purple urchin
XP_001631226  255 IDLLHKLLQKdPKKRLGAk--gAHELKAHPFFKAFDWADLAEKRIPAPFKP 303  starlet sea anemone
NP_001040645  255 RDFIGQLLEKkLEKRLGYn--gVDEIKNHKFMSSIDWDAAVKRTLKPVIVP 303  nematode
AAC67395      254 QDLLQRLLCKdPKKRLGAgpqgAQEVRNHPFFQGLDWVALAARKIPAPFRP 304  human

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