1L3R,1FOT,1U7E,1CDK,2JDS


Conserved Protein Domain Family
STKc_PKA

?
cd05580: STKc_PKA (this model, PSSM-Id:173671 is obsolete and has been replaced by 270732)
Click on image for an interactive view with Cn3D
Catalytic domain of the Protein Serine/Threonine Kinase, cAMP-dependent protein kinase
Serine/Threonine Kinases (STKs), cAMP-dependent protein kinase (PKA) subfamily, catalytic (c) subunit. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The PKA subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase (PI3K). This subfamily is composed of the cAMP-dependent proteins kinases, PKA and PRKX. The inactive PKA holoenzyme is a heterotetramer composed of two phosphorylated and active catalytic (C) subunits with a dimer of regulatory (R) subunits. Activation is achieved through the binding of the important second messenger cAMP to the R subunits, which leads to the dissociation of PKA into the R dimer and two active C subunits. PKA is present ubiquitously in cells and interacts with many different downstream targets. It plays a role in the regulation of diverse processes such as growth, development, memory, metabolism, gene expression, immunity, and lipolysis.
Statistics
?
PSSM-Id: 173671
View PSSM: cd05580
Aligned: 40 rows
Threshold Bit Score: 418.103
Threshold Setting Gi: 116057215
Created: 18-Jul-2006
Updated: 17-Jan-2013
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 42 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1L3R_E; Mouse PKA binds 20-aa substrate peptide and ADP-Mg-AlF3; defined at 4A contacts.
    View structure with Cn3D
  • Structure:1CDK_B; Sus scrofa PKA binds pseudosubstrate peptide inhibitor and MnAMP-PNP; defined at 4A contacts.
    View structure with Cn3D
  • Citation:PMID 8384554

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1               ###### #                                                           # # 
1L3R_E      41 DQFDRIKTLGTGSFGRVMLVKHke-----------------------------------------------sgNHYAMKI 73   house mouse
P51817      47 QDFDTLATVGTGTFGRVHLVKEkt-----------------------------------------------akHFFALKV 79   human
XP_638782  677 SDFKLFDLLGSGSFAKVRLCQHip-----------------------------------------------seRLFCMKI 709  Dictyostelium di...
AAG09429    15 KDLRHIKNIGSGSFGRVTLAQHid-----------------------------------------------tgKYYAVKA 47   Giardia intestin...
XP_951989  115 NDFEIGKTIGTGSYATVCIAKYtpnnryfeltrylypnfrergsvdsaytaysglssgqeyynnqvhdtetkeKIVSLKI 194  Theileria annula...
AAT81143   701 TELEFHRIIGTGQFGLVRVVRNik-----------------------------------------------tnEVYALKV 733  Chlamydomonas re...
CAL56443   406 KDVLFVKEIGSGMLGSVFIAKTln-----------------------------------------------tqETCCVKV 438  Ostreococcus tauri
BAE62818    74 DDFALLKTLGTGTFARVWLVRLkdei-------------------------------------------rqrdKVYALKI 110  Aspergillus oryzae
Q8SRK8       5 QDFEFVKVVGVGAFGKVHLVRLkss----------------------------------------------ptSVFAMKM 38   Encephalitozoon ...
AAC24243    49 SDFEVVETLGTGTFGRVLLVRLkdrdv------------------------------------------adrsAYFALKV 86   Ustilago maydis
Feature 1              #                      #               ## #   # #   #                   
1L3R_E      74 LDKqkvvkLKQIEHTLNEKRILQAVn-FPFLVKLEFSFKDnsNLYMVMEYVaGGEMFSHLRRi----gRFXEPHARFYAA 148  house mouse
P51817      80 MSIpdvirLKQEQHVHNEKSVLKEVs-HPFLIRLFWTWHDerFLYMLMEYVpGGELFSYLRNr----gRFSSTTGLFYSA 154  human
XP_638782  710 LNQnkiirLRQEVHVCNEKQVLMLTd-NPFIVKLYSTFKDdrYLYFLQEFIpGGELFDYIRAn----gSLSLYVTQIYAA 784  Dictyostelium di...
AAG09429    48 MSKrkiiaLRQVAHINNECSILRVVr-SPFIIRQYGTYQDdkNVYIILDFIqGGELFYHLRRy----nKFPLQVVKFFAV 122  Giardia intestin...
XP_951989  195 LSKdkiieKRQLEHVKNEKNILSSLk-HPFIVEYLGSFQDplNLYFVLEFVpGGELFTYLRRm----rNFPLEYTRFYSS 269  Theileria annula...
AAT81143   734 MHKapiveSKQIEHVINERKILEEActHPFCVRLCGAYQDkaSLYLLQEWVpGGELFHHLDVe----gAFDEPTAMFYAA 809  Chlamydomonas re...
CAL56443   439 MHKwkiahLDQEKNVCRELEIMKRFd-SPFLTRAVGAFQDarSLYLLMDYLpGGDLFQLLVSgpyrggRFPVNVAQFYAA 517  Ostreococcus tauri
BAE62818   111 LRKadvikLKQVEHVRNERKTLSAVagHPFITTLIASFSDdqSLYMLLDYCpGGEIFSYLRRa----rRFNENTAKFYAA 186  Aspergillus oryzae
Q8SRK8      39 LDFgeilrQRLADQLENEISILKRIygCPFVAKLYSTDFHggKVGLIMEYVgGGELFYWLKKc----gRFDEQMARFYAA 114  Encephalitozoon ...
AAC24243    87 LAKtdvikLKQVSHINSERCILTKVd-HPFLVNMIASFQDskNCYMLMEYVvGGEIFSYLRRa----gHFSADVARFYIS 161  Ustilago maydis
Feature 1                        # #### #         ##  #                    # ####              
1L3R_E     149 QIVLTFEYLHS-LDLIYRDLKPENLLIDqqGYIQVTDFGFAKRVKGR----------TWXLCGTPEYLAPEIILSkgYNK 217  house mouse
P51817     155 EIICAIEYLHS-KEIVYRDLKPENILLDrdGHIKLTDFGFAKKLVDR----------TWTLCGTPEYLAPEVIQSkgHGR 223  human
XP_638782  785 EIVLALEYLHN-QDIIYRDLKPENLLIDqyGHIKLTDFGFAKRITEN----------TKSMCGTPEYIAPEILSGhgHGK 853  Dictyostelium di...
AAG09429   123 EILLALGYLHN-LGIAYRDLKLENVLVDntGHIKLADLGFAKRLVEKnadgdtvsqlTFSIVGTPEYLAPEIIRStgHDM 201  Giardia intestin...
XP_951989  270 QVLLALDYLHM-NKLVYRDLKPENILLDvmGYIRIVDFGFAKRLDKGr---------TYTVCGTCDYLAPEIFLKkgHDF 339  Theileria annula...
AAT81143   810 NVLLALEFLHA-KGIVYRDLKPENLLLDtqGYIKMADFGFAKYIGGDk---------TYTICGTPDYQAPEVIMRrgTTK 879  Chlamydomonas re...
CAL56443   518 EVFIALEYIHT-EEYVYRDLKPENVLLDacGHVKLTDFGFCRHLLPGe--------rAYTTCGTADYMAPEVMLAqgYDQ 588  Ostreococcus tauri
BAE62818   187 EITLTIEFLHDvEGIVYRDLKPENILLDaeGHIRLVDFGFAKKVDNRe---------TYTLCGTPEYLAPEVIHNsgHGL 257  Aspergillus oryzae
Q8SRK8     115 EIISALRFIHG-RGILYRDLKPENILITstGHIKLIDFGFSVYESEN----------IYMISGTPEYMSPEKLRSedDGR 183  Encephalitozoon ...
AAC24243   162 TIVLAIEYLHS-NKVVYRDLKPENLLIDsnGYTKITDFGFAKEVEDR----------TWTLCGTPEYLAPEIIQCsgHGS 230  Ustilago maydis
Feature 1                  #   ###  ###   #  #                                                 
1L3R_E     218 AVDWWALGVLIYEMAAGYPPFFADq--PIQIYEKIVSGKVRFPSHf-sSDLKDLLRNLLQVdLTKRFGNlk-nGVNDIKN 293  house mouse
P51817     224 AVDWWALGILIFEMLSGFPPFFDDn--PFGIYQKILAGKIDFPRHl-dFHVKDLIKKLLVVdRTRRLGNmk-nGANDVKH 299  human
XP_638782  854 SADWWSLGILIYEMLVGVPPFVSEg-sQNDIFRLIREARIQVPPEv-dQVARDLIEKLVVTdVEKRLGSle-gGIEDIKN 930  Dictyostelium di...
AAG09429   202 SADWWAFGVLIYEMLTGSPPFFDDn--PDMTCKKILGGKITFASGf-dKAAKDLIIRLLNPdKTRRLGAsinnGTADIMK 278  Giardia intestin...
XP_951989  340 MADFYSFGVFLYELLIGIPPFYANt--PQKTYKLALNNEVKFSRKi-sEPSRDIIRKLLQVePSKRLGN----DVREVYF 412  Theileria annula...
AAT81143   880 AADYWALGVLIFEMLVGDPPFKSLtgdPWDTFRRTLSGRFYVPNFi-sDQAADLIFKLLQVnPDKRLGSdkrlGAEEIKR 958  Chlamydomonas re...
CAL56443   589 LADYWSFGVLLYEMLVGYAPFASVs--DGVRHRRIISSDLKFHSTyfsLTAKSLISRLCVVdVSKRLGSga-rGSGDLKS 665  Ostreococcus tauri
BAE62818   258 AVDWWALGILIYEFLVGQPPFWDQn--PMRIYEQIVEGRIRFPQNm-sPAAQNIISLLCKTnPTERLGYis-gGSARVKS 333  Aspergillus oryzae
Q8SRK8     184 ASDYWGLGVMIYEMLCGDPPFYDSs--ADAIYHKILESNVVFPHYv-sPVARCLITGLLDKnRATRLGTk---GICEIMG 257  Encephalitozoon ...
AAC24243   231 AVDWWSLGILLFEMLAGYPPFYDPn--PILIYEKILAGNLVFPEEi-dPLSRDLISSLLTAdRSRRLGNlr-gGANDVKN 306  Ustilago maydis
Feature 1                                                   #  #
1L3R_E     294 HKWFATTDWIAIYQRkveAPFIPKFKG------------PGDTSNFDDY 330  house mouse
P51817     300 HRWFRSVDWEAVPQRklkPPIVPKIAG------------DGDTSNFETY 336  human
XP_638782  931 HPFFGAINWNSIQNRe-sAPLKPRIRPlkhhlmderdeeDRISANFIKP 978  Dictyostelium discoideum AX4
AAG09429   279 HAFFSGVNCLGLRKKsgrHPIVPKLTD------------PADTANYEDY 315  Giardia intestinalis
XP_951989  413 HPFFEGIDFNMLLAKrikAPIVPTVSG------------QDDVSNFIKY 449  Theileria annulata strain Ankara
AAT81143   959 HRWFSRIDWRALEQKklpSPFRPRVRN------------PLDTSNFDNF 995  Chlamydomonas reinhardtii
CAL56443   666 HDFFRDLNWEDVTARridSPVRPPVRDr----------vSADVGSSAAS 704  Ostreococcus tauri
BAE62818   334 HPFFEDIQWDDLFYRrikGPIIPRVDH------------PADTGNFEEY 370  Aspergillus oryzae
Q8SRK8     258 HPFFKGIDWHEVESRriePPFIPNPNTvlss-----lasSGELKGTDDA 301  Encephalitozoon cuniculi
AAC24243   307 HPWFHGVDWKALQEGrilPPIVPYLGR------------PGDTSNFSKY 343  Ustilago maydis

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap