1L3R,1FOT,1U7E,1CDK,2JDS


Conserved Protein Domain Family
STKc_PKA

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cd05580: STKc_PKA (this model, PSSM-Id:173671 is obsolete and has been replaced by 270732)
Click on image for an interactive view with Cn3D
Catalytic domain of the Protein Serine/Threonine Kinase, cAMP-dependent protein kinase
Serine/Threonine Kinases (STKs), cAMP-dependent protein kinase (PKA) subfamily, catalytic (c) subunit. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The PKA subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase (PI3K). This subfamily is composed of the cAMP-dependent proteins kinases, PKA and PRKX. The inactive PKA holoenzyme is a heterotetramer composed of two phosphorylated and active catalytic (C) subunits with a dimer of regulatory (R) subunits. Activation is achieved through the binding of the important second messenger cAMP to the R subunits, which leads to the dissociation of PKA into the R dimer and two active C subunits. PKA is present ubiquitously in cells and interacts with many different downstream targets. It plays a role in the regulation of diverse processes such as growth, development, memory, metabolism, gene expression, immunity, and lipolysis.
Statistics
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PSSM-Id: 173671
View PSSM: cd05580
Aligned: 40 rows
Threshold Bit Score: 418.103
Threshold Setting Gi: 116057215
Created: 18-Jul-2006
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 42 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1L3R_E; Mouse PKA binds 20-aa substrate peptide and ADP-Mg-AlF3; defined at 4A contacts.
    View structure with Cn3D
  • Structure:1CDK_B; Sus scrofa PKA binds pseudosubstrate peptide inhibitor and MnAMP-PNP; defined at 4A contacts.
    View structure with Cn3D
  • Citation:PMID 8384554

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                  ###### #                                                           # # 
1L3R_E         41 DQFDRIKTLGTGSFGRVMLVKHke-----------------------------------------------sgNHYAMKI 73
gi 1709648     47 QDFDTLATVGTGTFGRVHLVKEkt-----------------------------------------------akHFFALKV 79
gi 66810117   677 SDFKLFDLLGSGSFAKVRLCQHip-----------------------------------------------seRLFCMKI 709
gi 9957600     15 KDLRHIKNIGSGSFGRVTLAQHid-----------------------------------------------tgKYYAVKA 47
gi 84994534   115 NDFEIGKTIGTGSYATVCIAKYtpnnryfeltrylypnfrergsvdsaytaysglssgqeyynnqvhdtetkeKIVSLKI 194
gi 50660932   701 TELEFHRIIGTGQFGLVRVVRNik-----------------------------------------------tnEVYALKV 733
gi 116061055  406 KDVLFVKEIGSGMLGSVFIAKTln-----------------------------------------------tqETCCVKV 438
gi 83772690    74 DDFALLKTLGTGTFARVWLVRLkdei-------------------------------------------rqrdKVYALKI 110
gi 51701600     5 QDFEFVKVVGVGAFGKVHLVRLkss----------------------------------------------ptSVFAMKM 38
gi 2565330     49 SDFEVVETLGTGTFGRVLLVRLkdrdv------------------------------------------adrsAYFALKV 86
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                 #                      #               ## #   # #   #                   
1L3R_E         74 LDKqkvvkLKQIEHTLNEKRILQAVn-FPFLVKLEFSFKDnsNLYMVMEYVaGGEMFSHLRRi----gRFXEPHARFYAA 148
gi 1709648     80 MSIpdvirLKQEQHVHNEKSVLKEVs-HPFLIRLFWTWHDerFLYMLMEYVpGGELFSYLRNr----gRFSSTTGLFYSA 154
gi 66810117   710 LNQnkiirLRQEVHVCNEKQVLMLTd-NPFIVKLYSTFKDdrYLYFLQEFIpGGELFDYIRAn----gSLSLYVTQIYAA 784
gi 9957600     48 MSKrkiiaLRQVAHINNECSILRVVr-SPFIIRQYGTYQDdkNVYIILDFIqGGELFYHLRRy----nKFPLQVVKFFAV 122
gi 84994534   195 LSKdkiieKRQLEHVKNEKNILSSLk-HPFIVEYLGSFQDplNLYFVLEFVpGGELFTYLRRm----rNFPLEYTRFYSS 269
gi 50660932   734 MHKapiveSKQIEHVINERKILEEActHPFCVRLCGAYQDkaSLYLLQEWVpGGELFHHLDVe----gAFDEPTAMFYAA 809
gi 116061055  439 MHKwkiahLDQEKNVCRELEIMKRFd-SPFLTRAVGAFQDarSLYLLMDYLpGGDLFQLLVSgpyrggRFPVNVAQFYAA 517
gi 83772690   111 LRKadvikLKQVEHVRNERKTLSAVagHPFITTLIASFSDdqSLYMLLDYCpGGEIFSYLRRa----rRFNENTAKFYAA 186
gi 51701600    39 LDFgeilrQRLADQLENEISILKRIygCPFVAKLYSTDFHggKVGLIMEYVgGGELFYWLKKc----gRFDEQMARFYAA 114
gi 2565330     87 LAKtdvikLKQVSHINSERCILTKVd-HPFLVNMIASFQDskNCYMLMEYVvGGEIFSYLRRa----gHFSADVARFYIS 161
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                           # #### #         ##  #                    # ####              
1L3R_E        149 QIVLTFEYLHS-LDLIYRDLKPENLLIDqqGYIQVTDFGFAKRVKGR----------TWXLCGTPEYLAPEIILSkgYNK 217
gi 1709648    155 EIICAIEYLHS-KEIVYRDLKPENILLDrdGHIKLTDFGFAKKLVDR----------TWTLCGTPEYLAPEVIQSkgHGR 223
gi 66810117   785 EIVLALEYLHN-QDIIYRDLKPENLLIDqyGHIKLTDFGFAKRITEN----------TKSMCGTPEYIAPEILSGhgHGK 853
gi 9957600    123 EILLALGYLHN-LGIAYRDLKLENVLVDntGHIKLADLGFAKRLVEKnadgdtvsqlTFSIVGTPEYLAPEIIRStgHDM 201
gi 84994534   270 QVLLALDYLHM-NKLVYRDLKPENILLDvmGYIRIVDFGFAKRLDKGr---------TYTVCGTCDYLAPEIFLKkgHDF 339
gi 50660932   810 NVLLALEFLHA-KGIVYRDLKPENLLLDtqGYIKMADFGFAKYIGGDk---------TYTICGTPDYQAPEVIMRrgTTK 879
gi 116061055  518 EVFIALEYIHT-EEYVYRDLKPENVLLDacGHVKLTDFGFCRHLLPGe--------rAYTTCGTADYMAPEVMLAqgYDQ 588
gi 83772690   187 EITLTIEFLHDvEGIVYRDLKPENILLDaeGHIRLVDFGFAKKVDNRe---------TYTLCGTPEYLAPEVIHNsgHGL 257
gi 51701600   115 EIISALRFIHG-RGILYRDLKPENILITstGHIKLIDFGFSVYESEN----------IYMISGTPEYMSPEKLRSedDGR 183
gi 2565330    162 TIVLAIEYLHS-NKVVYRDLKPENLLIDsnGYTKITDFGFAKEVEDR----------TWTLCGTPEYLAPEIIQCsgHGS 230
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                     #   ###  ###   #  #                                                 
1L3R_E        218 AVDWWALGVLIYEMAAGYPPFFADq--PIQIYEKIVSGKVRFPSHf-sSDLKDLLRNLLQVdLTKRFGNlk-nGVNDIKN 293
gi 1709648    224 AVDWWALGILIFEMLSGFPPFFDDn--PFGIYQKILAGKIDFPRHl-dFHVKDLIKKLLVVdRTRRLGNmk-nGANDVKH 299
gi 66810117   854 SADWWSLGILIYEMLVGVPPFVSEg-sQNDIFRLIREARIQVPPEv-dQVARDLIEKLVVTdVEKRLGSle-gGIEDIKN 930
gi 9957600    202 SADWWAFGVLIYEMLTGSPPFFDDn--PDMTCKKILGGKITFASGf-dKAAKDLIIRLLNPdKTRRLGAsinnGTADIMK 278
gi 84994534   340 MADFYSFGVFLYELLIGIPPFYANt--PQKTYKLALNNEVKFSRKi-sEPSRDIIRKLLQVePSKRLGN----DVREVYF 412
gi 50660932   880 AADYWALGVLIFEMLVGDPPFKSLtgdPWDTFRRTLSGRFYVPNFi-sDQAADLIFKLLQVnPDKRLGSdkrlGAEEIKR 958
gi 116061055  589 LADYWSFGVLLYEMLVGYAPFASVs--DGVRHRRIISSDLKFHSTyfsLTAKSLISRLCVVdVSKRLGSga-rGSGDLKS 665
gi 83772690   258 AVDWWALGILIYEFLVGQPPFWDQn--PMRIYEQIVEGRIRFPQNm-sPAAQNIISLLCKTnPTERLGYis-gGSARVKS 333
gi 51701600   184 ASDYWGLGVMIYEMLCGDPPFYDSs--ADAIYHKILESNVVFPHYv-sPVARCLITGLLDKnRATRLGTk---GICEIMG 257
gi 2565330    231 AVDWWSLGILLFEMLAGYPPFYDPn--PILIYEKILAGNLVFPEEi-dPLSRDLISSLLTAdRSRRLGNlr-gGANDVKN 306
                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*....
Feature 1                                                      #  #
1L3R_E        294 HKWFATTDWIAIYQRkveAPFIPKFKG------------PGDTSNFDDY 330
gi 1709648    300 HRWFRSVDWEAVPQRklkPPIVPKIAG------------DGDTSNFETY 336
gi 66810117   931 HPFFGAINWNSIQNRe-sAPLKPRIRPlkhhlmderdeeDRISANFIKP 978
gi 9957600    279 HAFFSGVNCLGLRKKsgrHPIVPKLTD------------PADTANYEDY 315
gi 84994534   413 HPFFEGIDFNMLLAKrikAPIVPTVSG------------QDDVSNFIKY 449
gi 50660932   959 HRWFSRIDWRALEQKklpSPFRPRVRN------------PLDTSNFDNF 995
gi 116061055  666 HDFFRDLNWEDVTARridSPVRPPVRDr----------vSADVGSSAAS 704
gi 83772690   334 HPFFEDIQWDDLFYRrikGPIIPRVDH------------PADTGNFEEY 370
gi 51701600   258 HPFFKGIDWHEVESRriePPFIPNPNTvlss-----lasSGELKGTDDA 301
gi 2565330    307 HPWFHGVDWKALQEGrilPPIVPYLGR------------PGDTSNFSKY 343

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