2F2U,2ESM,2VD5


Conserved Protein Domain Family
STKc_ROCK_NDR_like

?
cd05573: STKc_ROCK_NDR_like (this model, PSSM-Id:173664 is obsolete and has been replaced by 270725)
Click on image for an interactive view with Cn3D
Catalytic domain of ROCK- and NDR kinase-like Protein Serine/Threonine Kinases
Serine/Threonine Kinases (STKs), Rho-associated coiled-coil containing protein kinase (ROCK) and Nuclear Dbf2-Related (NDR)-like kinase subfamily, catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The ROCK- and NDR-like subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. Members of this subfamily include ROCK and ROCK-like proteins such as DMPK, MRCK, and CRIK, as well as NDR and NDR-like proteins such as LATS, CBK1 and Sid2p. ROCK and CRIK are effectors of the small GTPase Rho, while MRCK is an effector of the small GTPase Cdc42. NDR and NDR-like kinases contain an N-terminal regulatory (NTR) domain and an insert within the catalytic domain that contains an auto-inhibitory sequence. Proteins in this subfamily are involved in regulating many cellular functions including contraction, motility, division, proliferation, apoptosis, morphogenesis, and cytokinesis.
Statistics
?
PSSM-Id: 173664
View PSSM: cd05573
Aligned: 17 rows
Threshold Bit Score: 464.462
Threshold Setting Gi: 47156963
Created: 4-Aug-2006
Updated: 17-Jan-2013
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 31 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the binding of peptide substrates and ATP analogs to the AGC kinases, PKA and PKB.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                #####   #              # #                                              
2F2U_B        75 EDYDVVKVIGRGAFGEVQLVRHka--sqKVYAMKLLSKFEMikrsDSAFFWEERDIMAFAn------------------- 133
gi 1346360   206 GDFQTITQVGQGGYGSVWLARKrd--tkEIVALKIMNKSVLhkmdEIRHVLTERDILTTAn------------------- 264
gi 52783106  703 SMFVKIKTLGIGAFGEVCLARKvd--tkALYATKTLRKKDVllrnQVAHVKAERDILAEAd------------------- 761
2ESM_A        74 EDYEVVKVIGRGAFGEVQLVRHks--trKVYAMKLLSKFEMikrsDSAFFWEERDIMAFAn------------------- 132
gi 85691141   43 SDYEIVKNVAKGGYGEVFLVRRe-----KIYAMKRVSKELVlkqpHTALFMAEKEAMVDCi------------------- 98
gi 47156963   49 DDFEIVGLLARGAYGEVFVARRs----dAVYAIKRVLKETAvqqpFTALFMAEKELMVDAr------------------- 105
gi 70802736   67 DDFVLVKRIGKGAFGEVYLCYKkydndkQLYALKRMRKTDMirkkQISHVRSEKDVMAEAas------------------ 128
gi 13926114   52 NEFCVGDAIGRGAFAEVFQCWRpee-phIVYAMKKIRKDLIvkrkQSLNVHTERDLLSDAklrq---------------- 114
gi 66362004  146 DDFHTISVIGQGGYSIVRLVFDih--tgNVYALKQAKKCQImn-nQSQHDNLENLVKIDInsskkqveekqtlntgimln 222
gi 71016321  715 NSFLKLKTIGHGAFGVVALCKErq--sgQLYAMKQLRKADMlrkgQEGHVRAERDLMTSAsas----------------- 775
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                      #               ## #   # #                                     # #
2F2U_B       134 ----------SPWVVQLFCAFQDdkYLYMVMEYMpGGDLVNLMSNy-DVPEKWAKFYTAEVVLALDAIHS-MGLIHRDVK 201
gi 1346360   265 ----------SEWLVRLLYAFQDtsNIYLAMEFVpGGDFRTLLSNsgVLRDHHAKFYATEMFLAIDALHQ-LGYIHRDLK 333
gi 52783106  762 ----------NEWVVRLYYSFQDkdNLYFVMDYIpGGDMMSLLIRmgIFPESLARFYIAELTCAVESVHK-MGFIHRDIK 830
2ESM_A       133 ----------SPWVVQLFYAFQDdrYLYMVMEYMpGGDLVNLMSNy-DVPEKWARFYTAEVVLALDAIHS-MGFIHRDVK 200
gi 85691141   99 ---------gSEWLVCAHMTMQDeeYLYYLMDFIpGGDFMGLLSKedVLEEDWVRFYAAEIVAALDELHK-LGWIHRDLK 168
gi 47156963  106 ---------gSMWLLHAHWTFQDeeAVYYVTDFLpGGDFMGYLVRqeCVTEHEVRFYGAEIVAALEELHG-LGYVHRDLK 175
gi 70802736  129 ---------tNPWVVKLYLSFQDeqYLYMVMEYMpGGDMISWLCIkqRFDVESTRFYIAELCAAVASVHD-MFFVHRDIK 198
gi 13926114  115 ------krvsCPWITDLVVAFQDqdFLYIVTEYCsGGDLISWLIRydVFPEETARFYFVELVLALNALHK-MGYVRRDVK 187
gi 66362004  223 teislmynsdSPWINKLYYYWEDnhFYYMLMEYMpGGDLMRHLIDlhTFDEETAKFYIAELLLALDYLNNiQGHIHRDIK 302
gi 71016321  776 --------atAKWIVKLIYSFQDvdHLYLIMEFMgGGDLLNLLIEkdIFEEDFARFYVAEMVLAIHEAHS-FGYIHRDIK 846
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         ## #         ##  #                                                             
2F2U_B       202 PDNMLLDkhGHLKLADFGTCMKMDEtg----------------------------------------------------- 228
gi 1346360   334 PENFLVGasGHIKLTDFGLSSGIISkkkiesmkirlqevnnvvvp----------------------------------- 378
gi 52783106  831 PDNILIDrdGHIKLTDFGLCTGFRWthdskyyqsgdhprqdsmdfsnewg------------------------------ 880
2ESM_A       201 PDNMLLDksGHLKLADFGTCMKMNKeg----------------------------------------------------- 227
gi 85691141  169 PDNVLIGidGHVKLADFGSCIKMKDgk----------------------------------------------------- 195
gi 47156963  176 PENMLIDakGHIRLADFGSCARMADgs----------------------------------------------------- 202
gi 70802736  199 PDNILLDakGHIKLTDFGLSKRFAKvgeelldleegndalcssnssanlsntcgstcqgpn-------------svpvvs 265
gi 13926114  188 PDNVLIDreGHVKLADFGLSKRDPDqaestsvaddsyltedvt------------------------------------- 230
gi 66362004  303 PDNILLDvdGHIKLIDFGLSKQIIQskqrpfvsnasvnssf--------------------------------------- 343
gi 71016321  847 PDNFLYTsdGHIKLADFGLCQSFHWahdgayydqqrknllkkhgidlddshaaagmrraaqatgrrggapgqagaglsdk 926
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                         ######                              #     #  # 
2F2U_B       229 ---------------------------mvHCDTAVGTPDYISPEVLKSqggdgyYGRECDWWSVGVFLFEMLVGDTPFYA 281
gi 1346360   379 ---------ersmrerrqvfrtllsqdpvYAHSVVGSPDYMAPEVLRGe----nYNHSVDYWSLGCIMYECLSGFPPFSG 445
gi 52783106  881 ----dpsscrcgdrlkplerraarqhqrcLAHSLVGTPNYIAPEVLLRt----gYTQLCDWWSVGVILFEMLVGQPPFLA 952
2ESM_A       228 ---------------------------mvRCDTAVGTPDYISPEVLKSqggdgyYGRECDWWSVGVFLYEMLVGDTPFYA 280
gi 85691141  196 ----------------------------aRSSITVGTPDYVSPDVLCSvneeceYGEDVDFWTLGVIIYEMIYGTTPFYS 247
gi 47156963  203 ----------------------------vRCRATVGTPDYVSPEVLCGtgceveYGCEVDLWALGVVLYEMLYEAPPFYS 254
gi 70802736  266 phnphsptanynsltpagshasahpqarrIFESIVGSPGYIAPEILLRq----rYGVNCDWWSVGVIMYEMLYGIPPFFS 341
gi 13926114  231 ------------vdddvkkrfrdkkerkvMFFSTVGSPAYIAPEVLIGr----gYDYSCDWWSAGVILYEMIFGYPPFFN 294
gi 66362004  344 -------------qknvtaridphevlihDQNFHAGTPDYMAPEIHRGe----pYSLCVDLWSVGIILFEMLFGGPPLSD 406
gi 71016321  927 elieimsdrnadgtpkthvltwreknkkkIAYSVVGTNNYMAPEVLRGl----gYDQACDWWSLGVIVFEMLYGYPPFVS 1002
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2F2U_B       282 Ds--lVGTYSKIMdhkNSLCFPEdae-------isKHAKNLICAFLTdREVRLGrn------------------------ 328
gi 1346360   446 Sn--vNETWSNLKnwrKCFQRPHyddprdlefnwrDDAWDFVCHCITdPKDRFCs------------------------- 498
gi 52783106  953 Qt--pLETQMKVInwqTSLHIPPqak-------lsPEASDLIIKLCRgPEDRLGkn------------------------ 999
2ESM_A       281 Ds--lVGTYSKIMnhkNSLTFPDdnd-------isKEAKNLICAFLTdREVRLGrn------------------------ 327
gi 85691141  248 Dt--lMETYRRITr--VEFGFPFkv---------sPELTSLIEGLIAaKDRRMR-------------------------- 288
gi 47156963  255 Et--lQETYERISs--IDLRLPAhg---------sAEFRDLVVRLLCrREERLS-------------------------- 295
gi 70802736  342 Sd--tSSTCHKIThwrEYLTYPSdrg-------vpPEATDLLRRLMCdQRDRLD-------------------------- 386
gi 13926114  295 Dn--nTATAKKIIqfkEHLEFPKdqt------tvsKEAIDLISHLIAdSKERYG-------------------------- 340
gi 66362004  407 PgqnsLITRNKVMnwdKHFYLPSehh------kysPEAMDLICSLVCePSQRIKs------------------------- 455
gi 71016321 1003 Ks--rHLTRQKILnwkQTLRFPTkpr-------isREGQDFIARLVCeREDRLGstasasvsrpnsylqsarrsgfgnqg 1073
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2F2U_B       329 ----------gVEEIKQHPFFKNDQWNWdniretAAPVVPELSSDIDSSNFddieddkgdve------------------ 380
gi 1346360   499 -----------LKQVMQHPYFSKIDWKNvrt-ayRPPFVPDLNSEIDAGYFddftnendmskykevhe---------kqa 557
gi 52783106 1000 ----------gADEIKAHPFFKTIDFSSdlr-qqSASYIPKITHPTDTSNFdpvdpdklwsddneee----------nvn 1058
2ESM_A       328 ----------gVEEIKRHLFFKNDQWAWetlrdtVAPVVPDLSSDIDTSNFddleedkgeee------------------ 379
gi 85691141  289 -----------IEEIKSHAFFEGVDWSRlk--elKPPFVPEVNGDSDTSHFvdtqfdaek-------------------- 335
gi 47156963  296 -----------LEDVKAHAFFAGTDWESlh--rrQSPFVPKVMGDADMSNFerategstlae------------------ 344
gi 70802736  387 -----------FQGVKEHPFFNDIDWERlr--etPPAFVPELSDPLDTHYFpevedntpqqhhqr--------------- 438
gi 13926114  341 -----------FEEIIRHPFCKGVPLTDsir-neEAPFSVVLNDPKDLQYFepapdnadiqk------------------ 390
gi 66362004  456 -----------AKEALKHPFFKGMDFTKir--hqKPPIVPKVKHKLDHSNFdkfpddllvsfynsakastsv-pisnlse 521
gi 71016321 1074 qsntgsalqdgVEELMSHPWFRGIDWENlh--rtRPPFRPALSHPGDTKHFeddiedeplaapgaannpnadqprdpmlr 1151
                        490       500
                 ....*....|....*....|...
Feature 1                               
2F2U_B       381 --tfpipkafvgnQLPFIGFTYY 401
gi 1346360   558 aianmvntfnkpkRNAFIGFTFR 580
gi 52783106 1059 dtlngwykngkhpEHAFYEFTFR 1081
2ESM_A       380 --tfpipkafvgnQLPFVGFTYY 400
gi 85691141  336 -----rklnvkgnYMPFVGFTFD 353
gi 47156963  345 ---secgrggdahFLNFVGFTFD 364
gi 70802736  439 peernavrevdprGVIFADYKFN 461
gi 13926114  391 ---qpmtavsredQSVFVGFTSK 410
gi 66362004  522 elnfnscntnkiqGESFVSEFFP 544
gi 71016321 1152 dqeqgpkllemrkQLAFMGYTFK 1174

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap