Conserved Protein Domain Family
STKc_cGK_PKG

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cd05572: STKc_cGK_PKG (this model, PSSM-Id:173663 is obsolete and has been replaced by 270724)
Catalytic domain of the Protein Serine/Threonine Kinase, cGMP-dependent protein kinase
Serine/Threonine Kinases (STKs), cGMP-dependent protein kinase (cGK or PKG) subfamily, catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The cGK subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. Mammals have two cGK isoforms from different genes, cGKI and cGKII. cGKI exists as two splice variants, cGKI-alpha and cGKI-beta. cGK consists of an N-terminal regulatory domain containing a dimerization and an autoinhibitory pseudosubstrate region, two cGMP-binding domains, and a C-terminal catalytic domain. Binding of cGMP to both binding sites releases the inhibition of the catalytic center by the pseudosubstrate region, allowing autophosphorylation and activation of the kinase. cGKI is a soluble protein expressed in all smooth muscles, platelets, cerebellum, and kidney. It is also expressed at lower concentrations in other tissues. cGKII is a membrane-bound protein that is most abundantly expressed in the intestine. It is also present in the brain nuclei, adrenal cortex, kidney, lung, and prostate. cGKI is involved in the regulation of smooth muscle tone, smooth cell proliferation, and platelet activation. cGKII plays a role in the regulation of secretion, such as renin secretion by the kidney and aldosterone secretion by the adrenal. It also regulates bone growth and the circadian rhythm.
Statistics
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PSSM-Id: 173663
Aligned: 22 rows
Threshold Bit Score: 365.018
Created: 4-Aug-2006
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the binding of peptide substrates and ATP analogs to the AGC kinases, PKA and PKB.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1      #####   #                                                             # #       
Q13237     459 LGVGGFGRVELVKVkn-------------------------------------------------enVAFAMKCIRKKHI 489  human
AAM20901   690 VGRGTFGTVKLVRHvp-------------------------------------------------tdIRYALKCVSRRSV 720  Toxoplasma gondii
AAN36959   547 IGRGTFGTVKLVHHkp-------------------------------------------------tkIRYALKCVSKRSI 577  Plasmodium falci...
EAR88061   840 LGRGNFGSVNLVFNniqtqdfttnnkristqqdinqnfnirkskysqknpeiqiqkksnkfnekqkdRLFAMKVFISNYI 919  Tetrahymena ther...
CAH69750   455 IAYGQFGPVYLVKAky-------------------------------------------------nqQLYVLKAFNKNQI 485  Paramecium tetra...
CAH69645   475 LGSGQFGSVYLCRNkn-------------------------------------------------qeTLFALKYVTRAHI 505  Paramecium tetra...
CAD79353   463 LGEGTFGNAYLVKDip-------------------------------------------------qqNMHVMKCVPKVNV 493  Paramecium tetra...
BAB18105   380 LGGGAFSRVSIVTEes-------------------------------------------------tgRTYALKRMRKSAV 410  Chlamydomonas re...
CAL56612   281 LGGGTGGCVHKVISrr-------------------------------------------------dgRLFALKRVRKLAV 311  Ostreococcus tauri
CAL52451   630 LGVGSFGRVTLVSYq---------------------------------------------------gETYALKEIGKAQV 658  Ostreococcus tauri
Feature 1                              #               ## #   # #                              
Q13237     490 VdtKQQEHVYSEKRILEELCSPFIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGs---------------------- 547  human
AAM20901   721 IalSQQQHIRLEREIMAENDHPFIIRLVRTFRDKEFLYFLTELVTGGELYDAIRKLGl---------------------- 778  Toxoplasma gondii
AAN36959   578 InlNQQNNIKLEREITAENDHPFIIRLVRTFKDSKYFYFLTELVTGGELYDAIRKLGl---------------------- 635  Plasmodium falci...
EAR88061   920 FqrGIEKYILREKSTLQKCNFPFIMRYYRSFYDNYHIYFMNEFINGMDFFTVMREVGl---------------------- 977  Tetrahymena ther...
CAH69750   486 NeqTLEKYLQQEKQVLEIVNFPFIISFMKTFKDTLDVYFLLEYVRGMELFDVIRDIGl---------------------- 543  Paramecium tetra...
CAH69645   506 QqyGIQKHVQQEKSVLEIMNHQFILKFYRSFKDAENIYFLTEYIAGEELFDAIREIGl---------------------- 563  Paramecium tetra...
CAD79353   494 IanNTERHIKNEKQVLELLNHPNLVCFRRSFKDQNYIYLLTEYVKGKELFHVIREIGl---------------------- 551  Paramecium tetra...
BAB18105   411 V--QCPEHVFCEQAITKNVAHPFCIRQYASFQDKYHLYFLFDLMPGGDLMDVLVAEAkvikrrvpqgtwrigclapkvkm 488  Chlamydomonas re...
CAL56612   312 F--DAPAHIFCEKEVTSEISHFSLMCQHASFQDRAHLYMLFDLMDGCDLMDLLSSAVkvkmipsdvng-----qvtnvpt 384  Ostreococcus tauri
CAL52451   659 VsrGLVEHVWREKETMAQCDSPFLVNLHRTYVNEKSIFMLMDKVLGGELFTYLQMRNa---------------------- 716  Ostreococcus tauri
Feature 1                                      # # ## #         ##  #                ######    
Q13237     548 ---FDEPTSKFCVACVTEAFDYLHR-LGIIYRDLKPENLILDAEGYLKLVDFGFAKKIGSgq----kTWTFCGTPEYVAP 619  human
AAM20901   779 ---LARSQAQFYLASIVLAIEYLHE-RNIAYRDLKPENILLDSQGYVKLIDFGCAKKMQGr------AYTLVGTPHYMAP 848  Toxoplasma gondii
AAN36959   636 ---LSKSQAQFYLGSIILAIEYLHE-RNIVYRDLKPENILLDKQGYVKLIDFGCAKKVQGr------AYTLVGTPHYMAP 705  Plasmodium falci...
EAR88061   978 ---FNKQQAQFYTAFIILTLQYLNN-QGIVYRDLKPENILIDHEGWPKLVDMGTAKYLYDknqqltrTYSLVGTPHYMAP 1053 Tetrahymena ther...
CAH69750   544 ---LSTYDSQFYVASMILITEYLHH-QNIIYRDIKPENFMVDDKGFLKLIDLGTAKIVKGkqg-iirTYTIIGTPHYMAP 618  Paramecium tetra...
CAH69645   564 ---LGKYDSQFYTAQMILQMEYLHTvHQIVYRDIKPENVMVDQFGYLKLIDMGTAKSLKNvsp--pkTFTIIGTPHYMAP 638  Paramecium tetra...
CAD79353   552 ---LNSYETQFYIAQMINILEKLHS-QHIIYRDVKPENFIVMENGYIKLIDMGTAKVLKSra---skTYTLVGTPHYMAP 624  Paramecium tetra...
BAB18105   489 lqgMSEDLARFYIGSIVLALEYLHN-NNIVYRDLKPENVFIDASGYVKLGDFGFAKVLENgn----rTYTFCGTPGYVAP 563  Chlamydomonas re...
CAL56612   385 qmgMDEDAARYYCAMIVLAFEYLHA-NQIIYRDLKPENVLVGLNGRSKLGDFGYSRKLGVge----rAWTFCGTPGYVAP 459  Ostreococcus tauri
CAL52451   717 --pLPEAHGKFYAACVVSAFEYLHD-RNIIYRDLKPENLLISTNGYLKVTDFSFAKKLQKgq----kTFTLCGTPQYLAP 789  Ostreococcus tauri
Feature 1                            #     #  #                                                
Q13237     620 EVILNKGHDFSVDFWSLGILVYELLTGNPPFSGVd-------QMMTYNLILkgIEKMDFPRKi--tRRPEDLIRRLCRQN 690  human
AAM20901   849 EVILGKGYTLTADTWAFGVCLYEFMCGPLPFGNDae-----dQLEIFRDIL--TGKLVFPHYvt-dQDAINLMKRLLCRL 920  Toxoplasma gondii
AAN36959   706 EVILGKGYGCTVDIWALGICLYEFICGPLPFGNDee-----dQLEIFRDIL--TGQLTFPDYvt-dTDSINLMKRLLCRL 777  Plasmodium falci...
EAR88061  1054 EVIQQKGYGFAVDIYSLGVILYELLVGYLPYGEDvd-----dPIEVYQLIL--EGRLGFPNHmknrLSDKKLISQLMAKS 1126 Tetrahymena ther...
CAH69750   619 EIICGKGYNCLVDLWSIGICLYEFMCGMVPFGEEae-----dPYEIYEEII--KKDITYPNYlk-dKKAKKLMDQLLSRV 690  Paramecium tetra...
CAH69645   639 EVISGKGYGYFADLWSVGCCLYEFLCGGLPFGEEqe-----dPFEIYKEIV--KNPLHYPSYit-dKTAKTIIEQLLNKI 710  Paramecium tetra...
CAD79353   625 EILKGKGYSFSTDLWSLGICFYELMCGEVPFAGDee----ddPFIIEEAIL--NGKLEYPQIlk-dFKAIKLMDQLMNRT 697  Paramecium tetra...
BAB18105   564 ENVLAHGYNYSVDWWGLGVLMYVLLTGRQPFSTPkt----ddPMVVMRRIVdeNYQIKYPPYl--sPAAKDLISRLLERK 637  Chlamydomonas re...
CAL56612   460 EICLSKGYGFAVDWWALGVLTYVIITSRQPFTISkdpsqkddPIKVMRRIVdmTYKVQYPTYa--sDEACDFISQLLQRN 537  Ostreococcus tauri
CAL52451   790 EQVQQVGHNRAVDWWALGVLIYELVNGLPPFNQDd-------DYARYKAINd-VSFHFSPKNa---PAFRSVVSGLLLRL 858  Ostreococcus tauri
Feature 1                                  
Q13237     691 PTERLGnlkNGINDIKKHRWLNGFNWEG 718  human
AAM20901   921 PEVRIGcsiNGYKDIKEHAFFGDFDWDK 948  Toxoplasma gondii
AAN36959   778 PQGRIGcsiNGFKDIKDHPFFSNFNWDK 805  Plasmodium falciparum 3D7
EAR88061  1127 PEVRLG---GSYGALKSHEWFEDFDWDI 1151 Tetrahymena thermophila SB210
CAH69750   691 PEVRLG---GSYASLKGNPWFENFDWEK 715  Paramecium tetraurelia
CAH69645   711 PECRLG---GSYSSLKNNQWFSDFDWNK 735  Paramecium tetraurelia
CAD79353   698 PELRLG---GSFEALKVHPWFDGFQWEE 722  Paramecium tetraurelia
BAB18105   638 PAKRMGmlnARAMDIKNHKWFENLAWEE 665  Chlamydomonas reinhardtii
CAL56612   538 DMNRLGnvqGGARRIKEHPWFGKFDWEL 565  Ostreococcus tauri
CAL52451   859 PAGRLGmgkSGARDIKQHPWFADVNWSA 886  Ostreococcus tauri

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