Conserved Protein Domain Family
STKc_cGK_PKG

?
cd05572: STKc_cGK_PKG (this model, PSSM-Id:173663 is obsolete and has been replaced by 270724)
Catalytic domain of the Protein Serine/Threonine Kinase, cGMP-dependent protein kinase
Serine/Threonine Kinases (STKs), cGMP-dependent protein kinase (cGK or PKG) subfamily, catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The cGK subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. Mammals have two cGK isoforms from different genes, cGKI and cGKII. cGKI exists as two splice variants, cGKI-alpha and cGKI-beta. cGK consists of an N-terminal regulatory domain containing a dimerization and an autoinhibitory pseudosubstrate region, two cGMP-binding domains, and a C-terminal catalytic domain. Binding of cGMP to both binding sites releases the inhibition of the catalytic center by the pseudosubstrate region, allowing autophosphorylation and activation of the kinase. cGKI is a soluble protein expressed in all smooth muscles, platelets, cerebellum, and kidney. It is also expressed at lower concentrations in other tissues. cGKII is a membrane-bound protein that is most abundantly expressed in the intestine. It is also present in the brain nuclei, adrenal cortex, kidney, lung, and prostate. cGKI is involved in the regulation of smooth muscle tone, smooth cell proliferation, and platelet activation. cGKII plays a role in the regulation of secretion, such as renin secretion by the kidney and aldosterone secretion by the adrenal. It also regulates bone growth and the circadian rhythm.
Statistics
?
PSSM-Id: 173663
View PSSM: cd05572
Aligned: 22 rows
Threshold Bit Score: 365.018
Threshold Setting Gi: 74832250
Created: 4-Aug-2006
Updated: 17-Jan-2013
Structure
?
Aligned Rows:
 
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the binding of peptide substrates and ATP analogs to the AGC kinases, PKA and PKB.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1         #####   #                                                             # #       
gi 6226833    459 LGVGGFGRVELVKVkn-------------------------------------------------enVAFAMKCIRKKHI 489
gi 20378272   690 VGRGTFGTVKLVRHvp-------------------------------------------------tdIRYALKCVSRRSV 720
gi 23497415   547 IGRGTFGTVKLVHHkp-------------------------------------------------tkIRYALKCVSKRSI 577
gi 89290073   840 LGRGNFGSVNLVFNniqtqdfttnnkristqqdinqnfnirkskysqknpeiqiqkksnkfnekqkdRLFAMKVFISNYI 919
gi 74832334   455 IAYGQFGPVYLVKAky-------------------------------------------------nqQLYVLKAFNKNQI 485
gi 74832178   475 LGSGQFGSVYLCRNkn-------------------------------------------------qeTLFALKYVTRAHI 505
gi 29120036   463 LGEGTFGNAYLVKDip-------------------------------------------------qqNMHVMKCVPKVNV 493
gi 11230987   380 LGGGAFSRVSIVTEes-------------------------------------------------tgRTYALKRMRKSAV 410
gi 116061224  281 LGGGTGGCVHKVISrr-------------------------------------------------dgRLFALKRVRKLAV 311
gi 116061733  630 LGVGSFGRVTLVSYq---------------------------------------------------gETYALKEIGKAQV 658
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                 #               ## #   # #                              
gi 6226833    490 VdtKQQEHVYSEKRILEELCSPFIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGs---------------------- 547
gi 20378272   721 IalSQQQHIRLEREIMAENDHPFIIRLVRTFRDKEFLYFLTELVTGGELYDAIRKLGl---------------------- 778
gi 23497415   578 InlNQQNNIKLEREITAENDHPFIIRLVRTFKDSKYFYFLTELVTGGELYDAIRKLGl---------------------- 635
gi 89290073   920 FqrGIEKYILREKSTLQKCNFPFIMRYYRSFYDNYHIYFMNEFINGMDFFTVMREVGl---------------------- 977
gi 74832334   486 NeqTLEKYLQQEKQVLEIVNFPFIISFMKTFKDTLDVYFLLEYVRGMELFDVIRDIGl---------------------- 543
gi 74832178   506 QqyGIQKHVQQEKSVLEIMNHQFILKFYRSFKDAENIYFLTEYIAGEELFDAIREIGl---------------------- 563
gi 29120036   494 IanNTERHIKNEKQVLELLNHPNLVCFRRSFKDQNYIYLLTEYVKGKELFHVIREIGl---------------------- 551
gi 11230987   411 V--QCPEHVFCEQAITKNVAHPFCIRQYASFQDKYHLYFLFDLMPGGDLMDVLVAEAkvikrrvpqgtwrigclapkvkm 488
gi 116061224  312 F--DAPAHIFCEKEVTSEISHFSLMCQHASFQDRAHLYMLFDLMDGCDLMDLLSSAVkvkmipsdvng-----qvtnvpt 384
gi 116061733  659 VsrGLVEHVWREKETMAQCDSPFLVNLHRTYVNEKSIFMLMDKVLGGELFTYLQMRNa---------------------- 716
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                         # # ## #         ##  #                ######    
gi 6226833    548 ---FDEPTSKFCVACVTEAFDYLHR-LGIIYRDLKPENLILDAEGYLKLVDFGFAKKIGSgq----kTWTFCGTPEYVAP 619
gi 20378272   779 ---LARSQAQFYLASIVLAIEYLHE-RNIAYRDLKPENILLDSQGYVKLIDFGCAKKMQGr------AYTLVGTPHYMAP 848
gi 23497415   636 ---LSKSQAQFYLGSIILAIEYLHE-RNIVYRDLKPENILLDKQGYVKLIDFGCAKKVQGr------AYTLVGTPHYMAP 705
gi 89290073   978 ---FNKQQAQFYTAFIILTLQYLNN-QGIVYRDLKPENILIDHEGWPKLVDMGTAKYLYDknqqltrTYSLVGTPHYMAP 1053
gi 74832334   544 ---LSTYDSQFYVASMILITEYLHH-QNIIYRDIKPENFMVDDKGFLKLIDLGTAKIVKGkqg-iirTYTIIGTPHYMAP 618
gi 74832178   564 ---LGKYDSQFYTAQMILQMEYLHTvHQIVYRDIKPENVMVDQFGYLKLIDMGTAKSLKNvsp--pkTFTIIGTPHYMAP 638
gi 29120036   552 ---LNSYETQFYIAQMINILEKLHS-QHIIYRDVKPENFIVMENGYIKLIDMGTAKVLKSra---skTYTLVGTPHYMAP 624
gi 11230987   489 lqgMSEDLARFYIGSIVLALEYLHN-NNIVYRDLKPENVFIDASGYVKLGDFGFAKVLENgn----rTYTFCGTPGYVAP 563
gi 116061224  385 qmgMDEDAARYYCAMIVLAFEYLHA-NQIIYRDLKPENVLVGLNGRSKLGDFGYSRKLGVge----rAWTFCGTPGYVAP 459
gi 116061733  717 --pLPEAHGKFYAACVVSAFEYLHD-RNIIYRDLKPENLLISTNGYLKVTDFSFAKKLQKgq----kTFTLCGTPQYLAP 789
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                               #     #  #                                                
gi 6226833    620 EVILNKGHDFSVDFWSLGILVYELLTGNPPFSGVd-------QMMTYNLILkgIEKMDFPRKi--tRRPEDLIRRLCRQN 690
gi 20378272   849 EVILGKGYTLTADTWAFGVCLYEFMCGPLPFGNDae-----dQLEIFRDIL--TGKLVFPHYvt-dQDAINLMKRLLCRL 920
gi 23497415   706 EVILGKGYGCTVDIWALGICLYEFICGPLPFGNDee-----dQLEIFRDIL--TGQLTFPDYvt-dTDSINLMKRLLCRL 777
gi 89290073  1054 EVIQQKGYGFAVDIYSLGVILYELLVGYLPYGEDvd-----dPIEVYQLIL--EGRLGFPNHmknrLSDKKLISQLMAKS 1126
gi 74832334   619 EIICGKGYNCLVDLWSIGICLYEFMCGMVPFGEEae-----dPYEIYEEII--KKDITYPNYlk-dKKAKKLMDQLLSRV 690
gi 74832178   639 EVISGKGYGYFADLWSVGCCLYEFLCGGLPFGEEqe-----dPFEIYKEIV--KNPLHYPSYit-dKTAKTIIEQLLNKI 710
gi 29120036   625 EILKGKGYSFSTDLWSLGICFYELMCGEVPFAGDee----ddPFIIEEAIL--NGKLEYPQIlk-dFKAIKLMDQLMNRT 697
gi 11230987   564 ENVLAHGYNYSVDWWGLGVLMYVLLTGRQPFSTPkt----ddPMVVMRRIVdeNYQIKYPPYl--sPAAKDLISRLLERK 637
gi 116061224  460 EICLSKGYGFAVDWWALGVLTYVIITSRQPFTISkdpsqkddPIKVMRRIVdmTYKVQYPTYa--sDEACDFISQLLQRN 537
gi 116061733  790 EQVQQVGHNRAVDWWALGVLIYELVNGLPPFNQDd-------DYARYKAINd-VSFHFSPKNa---PAFRSVVSGLLLRL 858
                         330       340
                  ....*....|....*....|....*...
Feature 1                                     
gi 6226833    691 PTERLGnlkNGINDIKKHRWLNGFNWEG 718
gi 20378272   921 PEVRIGcsiNGYKDIKEHAFFGDFDWDK 948
gi 23497415   778 PQGRIGcsiNGFKDIKDHPFFSNFNWDK 805
gi 89290073  1127 PEVRLG---GSYGALKSHEWFEDFDWDI 1151
gi 74832334   691 PEVRLG---GSYASLKGNPWFENFDWEK 715
gi 74832178   711 PECRLG---GSYSSLKNNQWFSDFDWNK 735
gi 29120036   698 PELRLG---GSFEALKVHPWFDGFQWEE 722
gi 11230987   638 PAKRMGmlnARAMDIKNHKWFENLAWEE 665
gi 116061224  538 DMNRLGnvqGGARRIKEHPWFGKFDWEL 565
gi 116061733  859 PAGRLGmgkSGARDIKQHPWFADVNWSA 886

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap