1ZRZ,1XJD,2I0E


Conserved Protein Domain Family
STKc_PKC

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cd05570: STKc_PKC (this model, PSSM-Id:173661 is obsolete and has been replaced by 270722)
Click on image for an interactive view with Cn3D
Catalytic domain of the Protein Serine/Threonine Kinase, Protein Kinase C
Serine/Threonine Kinases (STKs), Protein Kinase C (PKC) subfamily, catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The PKC subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. PKCs are classified into three groups (classical, atypical, and novel) depending on their mode of activation and the structural characteristics of their regulatory domain. PKCs undergo three phosphorylations in order to take mature forms. In addition, classical PKCs depend on calcium, DAG (1,2-diacylglycerol), and in most cases, phosphatidylserine (PS) for activation. Novel PKCs are calcium-independent, but require DAG and PS for activity, while atypical PKCs only require PS. PKCs phosphorylate and modify the activities of a wide variety of cellular proteins including receptors, enzymes, cytoskeletal proteins, transcription factors, and other kinases. They play a central role in signal transduction pathways that regulate cell migration and polarity, proliferation, differentiation, and apoptosis. Also included in this subfamily are the PKC-like proteins, called PKNs.
Statistics
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PSSM-Id: 173661
View PSSM: cd05570
Aligned: 8 rows
Threshold Bit Score: 560.458
Threshold Setting Gi: 6225859
Created: 23-Aug-2006
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 31 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the binding of peptide substrates and ATP analogs to the AGC kinases, PKA and PKB.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          #####   #            # #                                  #               ## #
1ZRZ_A        26 RVIGRGSYAKVLLVRLkktdRIYAMKVVKKelvnddediDWVQTEKHVFEQAs--nHPFLVGLHSCFQTesRLFFVIEYV 103
1XJD_A        23 KMLGKGSFGKVFLAEFkktnQFFAIKALKKdvvlmdddvECTMVEKRVLSLAw--eHPFLTHMFCTFQTkeNLFFVMEYL 100
2I0E_A        26 MVLGKGSFGKVMLSERkgtdELYAVKILKKdvviqdddvECTMVEKRVLALPg--kPPFLTQLHSCFQTmdRLYFVMEYV 103
gi 6225859   661 AVLGRGHFGKVLLAEYkntnEMFAIKALKKgdivardevDSLMCEKRIFETVnsvrHPFLVNLFACFQTkeHVCFVMEYA 740
gi 80474928  412 KVLGKGSFGKVMLAELkgkdEVYAVKVLKKdvilqdddvDCTMTEKRILALAr--kHPYLTQLYCCFQTkdRLFFVMEYV 489
gi 125543    375 KVIGKGSFGKVLLAERrgtdELYAVKVLRKdviiqtddmELPMNEKKILALSg--rPPFLVSMHSCFQTmdRLFFVMEYC 452
gi 3114956   347 VVLGKGSFGKVFLAEQkttkDVYAIKSLKKdlivqeddvECTLNERKVLALQe--kPPFLTGLHSCFQTrdHLFFVMEYI 424
gi 281185512 359 RVLGKGSFGKVMLARVketgDLYAVKVLKKdvilqdddvECTMTEKRILSLAr--nHPFLTQLFCCFQTpdRLFFVMEFV 436
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1           # #                                    # # ## #         ##  #             ###
1ZRZ_A       104 nGGDLMFHMQRqrkLPEEHARFYSAEISLALNYLHeRGIIYRDLKLDNVLLDseGHIKLTDYGMCKEGLRPGDTTSXFCG 183
1XJD_A       101 nGGDLMYHIQSchkFDLSRATFYAAEIILGLQFLHsKGIVYRDLKLDNILLDkdGHIKIADFGMCKENMLGDAKTNXFCG 180
2I0E_A       104 nGGDLMYHIQQvgrFKEPHAVFYAAEIAIGLFFLQsKGIIYRDLKLDNVMLDseGHIKIADFGMCKENIWDGVTTKXFCG 183
gi 6225859   741 aGGDLMMHIHTd-vFSEPRAVFYAACVVLGLQYLHeHKIVYRDLKLDNLLLDteGFVKIADFGLCKEGMGYGDRTSTFCG 819
gi 80474928  490 nGGDLMFQIQRsrkFDEPRSRFYAAEVTSALMFLHqHGVIYRDLKLDNILLDaeGHCKLADFGMCKEGILNGVTTTTFCG 569
gi 125543    453 kGGDLMYHMQQygrFKESVAIFYAVEVAIALFFLHeRDIIYRDLKLDNILLDgeGHVKLVDFGLSKEGVTERQTTRTFCG 532
gi 3114956   425 nGGDLMFHILElgrFPENQTKFYAGEIVLGLRFLHkRGIIYRDLKLDNVMLDadGHIKIADFGLCKEGILGGNKTRTFCG 504
gi 281185512 437 nGGDLMFHIQKsrrFDEARARFYAAEIISALMFLHdKGIIYRDLKLDNVLLDheGHCKLADFGMCKEGICNGVTTATFCG 516
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ###                          #     #  #                                         
1ZRZ_A       184 TPNYIAPEILRGedyGFSVDWWALGVLMFEMMAGRSPFDIVgssdnpdqnteDYLFQVILEKQIRIPRSmsVKAASVLKS 263
1XJD_A       181 TPDYIAPEILLGqkyNHSVDWWSFGVLLYEMLIGQSPFHGQd---------eEELFHSIRMDNPFYPRWleKEAKDLLVK 251
2I0E_A       184 TPDYIAPEIIAYqpyGKSVDWWAFGVLLYEMLAGQAPFEGEd---------eDELFQSIMEHNVAYPKSmsKEAVAICKG 254
gi 6225859   820 TPEFLAPEVLTEtsyTRAVDWWGLGVLIYEMLVGESPFPGDd---------eEEVFDSIVNDEVRYPRFlsTEAISIMRR 890
gi 80474928  570 TPDYIAPEILQEleyGPSVDWWALGVLMYEMMAGQPPFEADn---------eDDLFESILHDDVLYPVWlsKEAVSILKA 640
gi 125543    533 TPNYMAPEIVSYdpySIAADWWSFGVLLFEFMAGQAPFEGDd---------eTTVFRNIKDKKAVFPKHfsVEAMDIITS 603
gi 3114956   505 TPDYIAPEIISYqpyDAAVDWWALGVLTYEMLVGRPPFDGDd---------dDQLFNNIMEKPVHYPRGmsDPAKKIIQG 575
gi 281185512 517 TPDYIAPEILQEmlyGPAVDWWAMGVLLYEMLCGHAPFEAEn---------eDDLFEAILNDEVVYPTWlhEDATGILKS 587
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1ZRZ_A       264 FLNkdPKERLGclpq-tgfADIQGHPFFRNVDWDMmeqKQVVPPFKPnisgefgldNFDSQFTNERVQLXPddddi-vrk 341
1XJD_A       252 LFVrePEKRLGv------rGDIRQHPLFREINWEElerKEIDPPFRPkvkspfdcsNFDKEFLNEKPRLSFadral-ins 324
2I0E_A       255 LMTkhPGKRLGcgp--egeRDIKEHAFFRYIDWEKlerKEIQPPYKPkacg-rnaeNFDRFFTRHPPVLXPpdqev-irn 330
gi 6225859   891 LLRrnPERRLGase--kdaEDVKKHPFFRLIDWSAlmdKKVKPPFIPtirgredvsNFDDEFTSEAPILTPpreprilse 968
gi 80474928  641 FMTknPHKRLGcvasqngeDAIKQHPFFKEIDWVLleqKKIKPPFKPriktkrdvnNFDQDFTREEPVLTLvdeai-vkq 719
gi 125543    604 FLTkkPNNRLGagr--yarQEITTHPFFRNVDWDKaeaCEMEPPIKPmikhrkdisNFDDAFTKEKTDLTPtdklf-mmn 680
gi 3114956   576 FLTkhPSRRLGchpe-tgeQDIKGAPFFKSIDWDRlgrRELKPPYKPkirgkksvsNFDPEFTKEPCRLSPvdptv-iga 653
gi 281185512 588 FMTknPTMRLGsltq-ggeHAILRHPFFKEIDWAQlnhRQIEPPFRPriksredvsNFDPDFIKEEPVLTPidegh-lpm 665
                        330
                 ....*....|..
Feature 1                    
1ZRZ_A       342 idQSEFEGFEYI 353
1XJD_A       325 mdQNMFRNFXFM 336
2I0E_A       331 idQSEFEGFXFV 342
gi 6225859   969 eeQEMFRDFDYI 980
gi 80474928  720 inQEEFKGFSYF 731
gi 125543    681 ldQNDFIGFSFM 692
gi 3114956   654 iePEVFEGFSFT 665
gi 281185512 666 inQDEFRNFSYV 677

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