1F3M,3CKX,1U5R,2CLQ,1S9J,2J7T,3COM,2VWI,2Q0N,1YHV


Conserved Protein Domain Family
PKc_STE

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cd05122: PKc_STE (this model, PSSM-Id:173659 is obsolete and has been replaced by 270692)
Click on image for an interactive view with Cn3D
Catalytic domain of STE family Protein Kinases
Protein Kinases (PKs), STE family, catalytic (c) domain. PKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine or tyrosine residues on protein substrates. The STE family is part of a larger superfamily that includes the catalytic domains of other protein serine/threonine kinases (STKs), protein tyrosine kinases (PTKs), RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase (PI3K). This family is composed of STKs, and some dual-specificity PKs that phosphorylate both threonine and tyrosine residues of target proteins. Most members are kinases involved in mitogen-activated protein kinase (MAPK) signaling cascades, acting as MAPK kinases (MAPKKs), MAPK kinase kinases (MAPKKKs), or MAPK kinase kinase kinases (MAP4Ks). The MAPK signaling pathways are important mediators of cellular responses to extracellular signals. The pathways involve a triple kinase core cascade comprising of the MAPK, which is phosphorylated and activated by a MAPKK, which itself is phosphorylated and activated by a MAPKKK. Each MAPK cascade is activated either by a small GTP-binding protein or by an adaptor protein, which transmits the signal either directly to a MAPKKK to start the triple kinase core cascade or indirectly through a mediator kinase, a MAP4K. Other STE family members include p21-activated kinases (PAKs) and class III myosins, among others. PAKs are Rho family GTPase-regulated kinases that serve as important mediators in the function of Cdc42 (cell division cycle 42) and Rac. Class III myosins are motor proteins containing an N-terminal kinase catalytic domain and a C-terminal actin-binding domain, which can phosphorylate several cytoskeletal proteins, conventional myosin regulatory light chains, as well as autophosphorylate the C-terminal motor domain. They play an important role in maintaining the structural integrity of photoreceptor cell microvilli.
Statistics
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PSSM-Id: 173659
Aligned: 30 rows
Threshold Bit Score: 256.751
Created: 30-Jun-2006
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 31 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:The active site is composed of the ATP binding site and the substrate binding site. The substrate binding site is based on the binding of human PAK4 to a consensus peptide.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1               #####   #                 # #                               #          
1F3M_C      21 KYTRF--EKIGQGASGTVYTAMDva-----tgQEVAIRQMNLqqq---pkkELIINEILVMRENkNPNIVNYLDSYLvg- 89   human
1U5R_B      55 LFSDL--REIGHGSFGAVYFARDvr-----nsEVVAIKKMSYsgkqsnekwQDIIKEVRFLQKLrHPNTIQYRGCYLre- 126  Norway rat
1359632    189 YFTNL--VSIGKGGFGEVFSLTQrie----dgKPIRFKMLKHsvd---eryTKIGAEVARLSNWkHPNIVGFEGCWLyn- 258  Entamoeba histol...
XP_657522  313 EVRRL--ESVGKGGFGEVFRAQRks-----dgTVVALKILKHtvk---erySKLGQEIARMALWkHEFLIQIDKCYLfd- 381  Entamoeba histol...
XP_646541  398 KKRIKftHMVGRGQYGKVYDALYd-------kKRVCVKVVNYstp---keqHNVLQEIGFLTQCdHPNILKYNCSVLyg- 466  Dictyostelium di...
XP_629713  934 LFRDK--IKLGTGAFGNVFYAIRks-----dsSPVAIKVLMErtk----kdSPIIPELYIHSACnHSNIVTYIESYLck- 1001 Dictyostelium di...
1405366    584 LYTFR--EKVGKGGFGTVYLVRNnmd---kskSRIAIKKLPHvrk---kekKFNVKEIRVLEFTnHPNIITYYNSHMlh- 654  Dictyostelium di...
XP_635572  437 MYKDS--DQVGKGGFGTVYFAKStk-----ekRLVAIKKMPHvtk---rqqQQNFREAAILAKCdHPNIVKLHTCHIdkd 506  Dictyostelium di...
XP_640557  425 LFRIGe-NAEVKGAFGTVYQVFYvngq--ynnVDVALKKMDHkse---kkrRNNLNEISILRYLkHPNIVTYINSYEknd 498  Dictyostelium di...
XP_642589  368 HFKNL--DFEARGGFGSVFCAKNknphsaydkQMVALKKMPIktl---rhkRMNLSEIGFLKYFnHPNIVKFLCAYQksn 442  Dictyostelium di...
Feature 1            ####  ##  #                                            # #### #          #
1F3M_C      90 deLWVVMEYLagGSLTDVVtet----------cmDEGQIAAVCRECLQALEFLHsNQVIHRDIKSDNILLGmdGSVKLTD 159  human
1U5R_B     127 htAWLVMEYCl-GSASDLLevhk--------kplQEVEIAAVTHGALQGLAYLHsHNMIHRDVKAGNILLSepGLVKLGD 197  Norway rat
1359632    259 nqVYIGMEYCslGTLKTLLknrm--------rpfQDADTAFLLLETLKALKYIHeEGFIHRDIKTANIMLKqnFEIKVID 330  Entamoeba histol...
XP_657522  382 nrVYITMPYCscGSIKQVVkdks--------hifTLCDVSYVIQCLLMALEYIHsQGFIHRDIKPANILMMddGGVKLID 453  Entamoeba histol...
XP_646541  467 sdLFIVSEFIqgGTLEQASass---------hvfKETQVGFIGLELLKAISYLHeKKLIHRDIKAANVMVStdGEVKLID 537  Dictyostelium di...
XP_629713 1002 ghVWIILEYCdgGTVRDLLqatctpgnpnnlqlfEETLIAYIITELLEGLVYLRsKGIIHRDLKSRNILLTrkGKVKIAD 1081 Dictyostelium di...
1405366    655 deVWIAMEFMegGTLAEASnqy----------pfQESNIAYVAREVLQGLAYLHgIQLVHRDLKSQNIMMTtsGEIKLID 724  Dictyostelium di...
XP_635572  507 snLWIVMEFMegGTFEEAAkaw----------kfNENNLAYVAKELLKGLQYLHeNHMVHRDLKSANIMMSveGKVKLID 576  Dictyostelium di...
XP_640557  499 eeIWMVMEYMdgGTIRDAIsnf----------tfTEKYVAYITKEILHSLEYLAsLNIAHRDLKSSNIMINskAEVKLID 568  Dictyostelium di...
XP_642589  443 deLWMIMEFLsgGTLKNAAsnf----------kfCERKIAYVCREILQGLDYLHkQNIAHRDLKSANVMVNdkGEIKLID 512  Dictyostelium di...
Feature 1        #             #### #                             #        #                   
1F3M_C     160 FGFCAQITpeqskrstMVGTPYWMAPEVVTRka---yGPKVDIWSLGIMAIEMIegEPPYLnenplRALYLIat---nGT 233  human
1U5R_B     198 FGSASIMApa----nxFVGTPYWMAPEVILAmdegqyDGKVDVWSLGITCIELAerKPPLFnmnamSALYHIaq---nES 270  Norway rat
1359632    331 FGLVVRINskp---snRAGSKAYMAPEVIKQip---yNEKVDIWSIGCVAQELFegSPPYRelgmfKGLFKTat---vGA 401  Entamoeba histol...
XP_657522  454 FGLVVRKSlnp---htRSGSKQYMAPEVVLQkv---yDEKADIWSVGCVAQELAegKSPYRedgivKLLFRTvt---nGA 524  Entamoeba histol...
XP_646541  538 FGLCASVEkgg--sqhMVGSPYYMSPEMIRGee---cSYPSDIWSFGICILELLfkKPPHRds-rmKAMFYNain-giDF 610  Dictyostelium di...
XP_629713 1082 FGLATTCSlgrg-rtrMCGTMGRIAPEVIRRep---yDTQSDIYSLGCLIIEMAegTVPYGkdsslKALFYTa----iHQ 1153 Dictyostelium di...
1405366    725 FGLCANLSkge--rirMCGSPIWMPPEMIQQkq---hGYTCDIWSTAICLLELAnrNNKLRkn-piKTMFMVgsegikDP 798  Dictyostelium di...
XP_635572  577 FGLCEDVAtst--pmhMVGSPFWMAPEMIQQky---hSTPVDIWSFAISLLEMAnqRPPMMes-avKAMFTVat---dGA 647  Dictyostelium di...
XP_640557  569 FGFSIDLThlkq-dinMCGSPFYMSPEQIQDka---hGLAVDIWSLGIVVAEMVrgRVPHHks-kiKAMFLAgt---vGV 640  Dictyostelium di...
XP_642589  513 FGLCIDFSieke-einMLGSPSYISPEMINGnp---hSLSTDIWSFGICALEMLlgKLPYHds-rlKAMVFVatn-nlNL 586  Dictyostelium di...
Feature 1                                                
1F3M_C     234 PELQNPek---lsAIFRDFLNRCLDMdVEKRGSaKELLQHQF 272  human
1U5R_B     271 PALQSGhw----sEYFRNFVDSCLQKiPQDRPTsEVLLKHRF 308  Norway rat
1359632    402 PGLRNPsk---apPELVDFLKKCFFFnPEERWSaTQLLEHPF 440  Entamoeba histolytica
XP_657522  525 MGLRRIky---wdNIFVDFVEQCFIYnPEKRWSaTQLLKHPF 563  Entamoeba histolytica HM-1:IMSS
XP_646541  611 PKIRCS-------IDLKDMLWQCFESnPEKRSTvDKLMRHPF 645  Dictyostelium discoideum AX4
XP_629713 1154 YKLPNPkk---ytKEFVDFLYLCLNPdPFKRPTpEMLLHHTF 1192 Dictyostelium discoideum AX4
1405366    799 FEDPHKw-----sDEFHDIINKCLQMdPHKRPSaQELLKHPF 835  Dictyostelium discoideum
XP_635572  648 TGFDDPal---wsDCFKDFLSLCLKQdPAERATaEELLKHPF 686  Dictyostelium discoideum AX4
XP_640557  641 KFSKEKkyschwsPELFDFLNVCLQMdPTKRPTpTQLLQHPF 682  Dictyostelium discoideum AX4
XP_642589  587 PLLLSTtt---ssLEFRDFLTNCLQFdPSKRLTsSQLLQHPF 625  Dictyostelium discoideum AX4

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