2GSF,2HEL


Conserved Protein Domain Family
PTKc_EphR_A

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cd05066: PTKc_EphR_A (this model, PSSM-Id:173639 is obsolete and has been replaced by 270651)
Click on image for an interactive view with Cn3D
Catalytic domain of the Protein Tyrosine Kinases, Class EphA Ephrin Receptors
Protein Tyrosine Kinase (PTK) family; Ephrin Receptor (EphR) subfamily; most class EphA receptors including EphA3, EphA4, EphA5, and EphA7, but excluding EphA1, EphA2 and EphA10; catalytic (c) domain. The PTKc family is part of a larger superfamily that includes the catalytic domains of other kinases such as protein serine/threonine kinases, RIO kinases, and phosphoinositide 3-kinase (PI3K). PTKs catalyze the transfer of the gamma-phosphoryl group from ATP to tyrosine (tyr) residues in protein substrates. EphRs comprise the largest subfamily of receptor tyr kinases (RTKs). In general, class EphA receptors bind GPI-anchored ephrin-A ligands. There are ten vertebrate EphA receptors (EphA1-10), which display promiscuous interactions with six ephrin-A ligands. One exception is EphA4, which also binds ephrins-B2/B3. EphRs contain an ephrin-binding domain and two fibronectin repeats extracellularly, a transmembrane segment, and a cytoplasmic tyr kinase domain. Binding of the ephrin ligand to EphR requires cell-cell contact since both are anchored to the plasma membrane. The resulting downstream signals occur bidirectionally in both EphR-expressing cells (forward signaling) and ephrin-expressing cells (reverse signaling). Ephrin/EphR interaction mainly results in cell-cell repulsion or adhesion, making it important in neural development and plasticity, cell morphogenesis, cell-fate determination, embryonic development, tissue patterning, and angiogenesis. EphARs and ephrin-A ligands are expressed in multiple areas of the developing brain, especially in the retina and tectum. They are part of a system controlling retinotectal mapping.
Statistics
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PSSM-Id: 173639
View PSSM: cd05066
Aligned: 13 rows
Threshold Bit Score: 588.793
Threshold Setting Gi: 55962854
Created: 17-May-2007
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 22 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the structures of other PTK family members bound to substrate peptides and ATP analogs including, the structures of human EphA2 receptor bound with an ATP analog and mouse EphB2 receptor tyr kinase bound with ADP, (1MQB_B, 2HEN_A respectively).
  • Citation:PMID 9312016

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    # ###   #               # #                                          
2GSF_A         42 LDATNISIDKVVGAGEFGEVCSGRLKLpskKEISVAIKTLKVGYTEkQRRDFLGEASIMGQFDHPNIIRLEGVVTksKPV 121
2HEL_A         26 IDASCIKIEKVIGVGEFGEVCSGRLKVpgkREICVAIKTLKAGYTDkQRRDFLSEASIMGQFDHPNIIHLEGVVTkcKPV 105
gi 119568903  623 LDASCIKIERVIGAGEFGEVCSGRLKLpgkRDVAVAIKTLKVGYTEkQRRDFLCEASIMGQFDHPNVVHLEGVVTrgKPV 702
gi 55962854   359 IEASRIKIEKIIGSGEFGEVCYGRMKLpgkRDVPVALKTLKAGYTEkQRRDFLAEASIMAQFDHPNVIHLEGVVTrsKPV 438
gi 119600293  729 IDPSRIRIERVIGAGEFGEVCSGRLKTpgkREIPVAIKTLKGGHMDrQRRDFLREASIMGQFDHPNIIRLEGVVTksRPV 808
gi 45384174   623 LDASCIKIERVIGAGEFGEVCSGRLKLpgkRDVAVAIKTLKVGYTEkQRRDFLCEASIMGQFDHPNVVHLEGVVTrgKPV 702
gi 123704646  646 LDASCIKIERVIGAGEFGEVCSGRLKLpgkRDVSVAIKTLKVGYTEkQRRDFLCEASIMGQFDHPNVVHLEGVVTrgKPV 725
gi 45382181   616 LDASNISIDKVVGAGEFGEVCSGRLKLpskKEISVAIKTLKAGYTEkQRRDFLGEASIMGQFDHPNIIRLEGVVTksKPV 695
gi 47226254   596 LDASCIAIDKVVGAGEFGEVCSGRLKLpskKEICVAIKTLKGGYTDkQRRDFLAEASIMGQFDHPNIIRLEGVVTrsKPV 675
gi 555619     624 IEASCITIERVIGAGEFGEVCSGRLKLqgkREFPVAIKTLKVGYTEkQRRDFLGEASIMGQFDHPNIIHLEGVVTksKPV 703
gi 8134439    615 IDASCIKIEKVIGVGEFGEVCSGRLKVpgkREIYVAIKTLKAGYTDkQRRDFLSEASIMGQFDHPNIIHLEGVVTkcKPV 694
gi 312217     479 IDASCIKIEKVIGVGEFGEVCSGRLKVpgkREICVAIKTLKAGYTDkQRRDFLSEASIMGQFDHPNIIHLEGVVTkcKPV 558
gi 24119222   617 IDASTIRIERVIGAGEFGEVCSGRLKLpskREIYVAIKSLKAGYSEkQRRDFLSEASIMGQFDHPNIIRLEGVVTrcKPV 696
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            ## #                                           #   ## #          #           
2GSF_A        122 MIVTEYMENGSLDSFLRKHdaqFTVIQLVGMLRGIASGMKYLSDMGYVHRDLAARNILINSnLVCKVSDFGLSRVLEDDP 201
2HEL_A        106 MIITEYMENGSLDAFLRKNdgrFTVIQLVGMLRGIGSGMKYLSDMSAVHRDLAARNILVNSnLVCKVSDFGMSRVLEDDP 185
gi 119568903  703 MIVIEFMENGALDAFLRKHdgqFTVIQLVGMLRGIAAGMRYLADMGYVHRDLAARNILVNSnLVCKVSDFGLSRVIEDDP 782
gi 55962854   439 MIITEYMENGSLDSFLRRHdgqFTIIQLVGILRGIAAGMTYLADLGYVHRDLAARNVLVNSnLVCKISDFGLSRVLEDDP 518
gi 119600293  809 MIVVEYMENGSLDSFLRKHdghFTVIQLVGMLRGIASGMKYLSDMGYVHRDLAARNILVNSnLVCKVSDFGLSRVLEDDP 888
gi 45384174   703 MIVIEYMENGALDAFLRKHdgqFTVIQLVGMLRGIAAGMRYLADMGYVHRDLAARNILVNSnLVCKVSDFGLSRVIEDDP 782
gi 123704646  726 MIVIEYMENGSLDAFLRKHdgqFTVIQLVGMLRGIAAGMRYLSDMGYVHRDLAARNILVNSnLVCKVSDFGLSRVIDDDP 805
gi 45382181   696 MIVTEYMENGSLDSFLRKHdaqFTVIQLVGMLRGIASGMKYLSDMGYVHRDLAARNILINSnLVCKVSDFGLSRVLEDDP 775
gi 47226254   676 MIVTEYMENGSLDTFLRKHdaqFTGIQLVGMLRGIASGMKYLSDMGYVHRDLAARNILVNSnLVCKVSDFGLSRVLEDDP 755
gi 555619     704 MIVTEYMENGSLDTFLKKNdgqFTVIQLVGMLRGIASGMKYLSDMGYVHRDLAARNILINSnLVCKVSDFGLSRVLEDDP 783
gi 8134439    695 MIITEYMENGSLDAFLRKNdgrFTVIQLVGMLRGIGSGMKYLSDMSYVHRDLAARNILVNSnLVCKVSDFGMSRVLEDDP 774
gi 312217     559 MIITEYMENGSLDAFLRKNdgrFTVIQLVGMLRGIGSGMKYLSDMSYVHRDLAARNILVNSnLVCKVSDFGMSRVLEDDP 638
gi 24119222   697 MIITEYMENGSLDTFLKKHdgqFTVIQLVGMMRGIASGMKYLSDMSYVHRDLAARNILVNSnLVCKVSDFGLSRVLEDDP 776
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                #####        #                                 #                         
2GSF_A        202 EAAYTTRGGKIPIRWTSPEAIAYrKFTSASDVWSYGIVLWEVMSYGERPYWEMSNQDVIKAVDEGYRLPPPMDCPAALYQ 281
2HEL_A        186 EAAYTTRGGKIPIRWTAPEAIAYrKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPPPMDCPIALHQ 265
gi 119568903  783 EAVYTTTGGKIPVRWTAPEAIQYrKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPAPMDCPAGLHQ 862
gi 55962854   519 DAAYTTSGGKIPIRWTAPEAIAYrKFSSSSDVWSYGVVMWEVMSYGEQPYWNLTNRDVIKSVEEGYRLPAPMGCPGALHT 598
gi 119600293  889 EAAYTTTGGKIPIRWTAPEAIAYrKFSSASDAWSYGIVMWEVMSYGERPYWEMSNQDVILSIEEGYRLPAPMGCPASLHQ 968
gi 45384174   783 EAVYTTTGGKIPVRWTAPEAIQYrKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPAPMDCPAGLHQ 862
gi 123704646  806 EAVYTTTGGKIPVRWTAMEAIQYrKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPAPMDCPPGLHQ 885
gi 45382181   776 EAAYTTRGGKIPIRWTSPEAIAYrKFTSASDAWSYGIVLWEVMSYGERPYWEMSFQDVIKAVDEGYRLPPPMDCPAALYQ 855
gi 47226254   756 EAAYTTRGGKIPIRWTAPEAIAYrKFTSASDAWSYGIVLWEVMSYGERPYWEMSNQDVIKAVDEGYRLPPPMDCPATLYQ 835
gi 555619     784 EAAYTTRGGKIPIRWTAPEAIAFrKFTSASDVWSYGIVMWEVMSYGERPYWEMTNQDVIKAVEEGYRLPSPMDCPAALYQ 863
gi 8134439    775 EAAYTTRGGKIPIRWTAPEAIAYrKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPPPMDCPIALHQ 854
gi 312217     639 EAAYTTRGGKIPIRWTAPEAIAYrKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPPPMDCPIALHQ 718
gi 24119222   777 EAAYTTRGGKIPIRWTAPEAIAYrKFTSASDVWSYGIVMWEVISYGERPYWEMSNQDVIKAIDEGYRLPAPMDCPVVLHQ 856
                         250       260
                  ....*....|....*....|....*..
Feature 1                                    
2GSF_A        282 LMLDCWQKdRNNRPKFEQIVSILDKLI 308
2HEL_A        266 LMLDCWQKeRSDRPKFGQIVNMLDKLI 292
gi 119568903  863 LMLDCWQKeRAERPKFEQIVGILDKMI 889
gi 55962854   599 LMLDCWQKdRNERPKFCQIVTVLDKLI 625
gi 119600293  969 LMLHCWQKeRNHRPKFTDIVSFLDKLI 995
gi 45384174   863 LMLDCWQKeRGERPKFEQIVGILDKMI 889
gi 123704646  886 LMLDCWQKdRADRPKFDQIVGILDKMI 912
gi 45382181   856 LMLDCWQKdRNNRPKFEQIVSILDKLI 882
gi 47226254   836 LMLDCWQKeRNNRPKFEQIVSILDKLI 862
gi 555619     864 LMLDCWQKdRNSRPKFDEIVSMLDKLI 890
gi 8134439    855 LMLDCWQKdRSDRPKFGQIVSMLDKLI 881
gi 312217     719 LMLDCWQKeRSDRPKFGQIVNMLDKLI 745
gi 24119222   857 LMLDCWEKsRSERPKFGQIVNTLDKLI 883

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