2G3M


Conserved Protein Domain Family
GH31_glucosidase_II_MalA

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cd06604: GH31_glucosidase_II_MalA (this model, PSSM-Id:133135 is obsolete and has been replaced by 269890)
Click on image for an interactive view with Cn3D
Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source.
Statistics
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PSSM-Id: 133135
View PSSM: cd06604
Aligned: 46 rows
Threshold Bit Score: 484.745
Threshold Setting Gi: 74563758
Created: 11-Sep-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                         #                           #                                  
2G3M_A       167 GKPFLPPMWAFGYMISRYSYYPQDKVVELVDIMQKegfRVAGVFLDIHYMDSYKLFTWHPyRFPEPKKLIDELHKRNVKL 246
gi 74563758  172 GKPFLPPNWGLGLHISRYSYEPHNAVVEVVEEVIEa-vPVDAVYLDIDYMDRYKQFTWDArKFPDPRGFVEQVHELGARV 250
gi 48477164  163 GRTFVPPRWALGHQISRYSYYPDRTVINVVKEYRKy-iDVSAVYLDIDYMDDYKIFTFDKeRFPDIKKFKEELNAMGTRL 241
gi 41033649  179 GKPFLPPRWALGYQISRYSYEPQDYVLMVVDKLLSe-vPLDAVYLDIDHMEDYKIFTWDRrKFPSPHELISELHGRGVRV 257
gi 70606930  164 GKPFELPEWALGYQISRYSYYPQETVEEVVRRHLEediPLSAIYLDIDYMEKYRLFTWDKaKFPSPKELIEKLHSLGVKV 243
gi 159042253 194 GKPTLMPKWALGHQQSRYSYYPQDRVIEIIKTFKEkelDNTVVYLDIHYMDGYRIFTWSKdRFPNPTELAKAAHELGVKL 273
gi 55978216  252 GLPPMPPRWALGFHYARWGLRTREEVEERVAGFLErglPLRAVHLDIDYMRGYRVFTVDEgRYPDLQGLVRGFQEKGVRT 331
gi 37521104  277 GRPPLPPKWALGYHQSRWGYETEAAVRNVAQAFEAndiPVSAIHLDIDVMDDFKAFTIDPkRFPLLGEFTRDLARRGVRL 356
gi 17232823  280 GRPPLPPRWTFGYHQSRWGYEREAALREVVKGFETyniPVSALHLDIDVLDNFRAFTIDPdRFPHLPELAAELAAKGIRL 359
gi 29833511  288 GAPALPPAWALGHHHARRGSGSEQEVRGVVSGYQErglPLDAVHLDNDHYDAHQVFTVDRdRFPKLPVLAEELRRDGIRL 367
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #                                  #                                  # #   
2G3M_A       247 ITIVDHGIRVDQnYSPFLSGm--GKFCEIESGELFVGKMWPGTTVYPDFFREDTREWWAGLISEwl-sqGVDGIWLDMNE 323
gi 74563758  251 VAILDPYIKAEPgYKPFEKLl--DCFLVTENDEIYLARGWPGLSALPDFLKPECREKWAALVAEfvsnyNIDGIWLDMNE 328
gi 48477164  242 ITIIDPGFKIDQlYKYFINGi--GKYVINSNNEIYISRLWPGNCAFLNFLDADSYNYWKSCVKEfa--eNVDGIWLDMNE 317
gi 41033649  258 VPIVDPYVKVEPgYRVFEGGl--RYMMTTKRNELYIARGWPGLSTLPDFLNRKTREWWAQLVEAyvkeyDVDGIWLDMNE 335
gi 70606930  244 VTIVDPCVRLDQnYHVFKDGl--GNYVENEDGTIYADILWPGLSVFPDFLNSKTREWWRNLVEKwvkenNIDGIWLDMNE 321
gi 159042253 274 VTIVDPYVKVDPnYYVFKEGingNHLSLDDDGGLSIVQGWPGKSALPDFFNKEAREWWASLIERwvreyGVDGIWLDMNE 353
gi 55978216  332 VLILDPGVKAEKgFPPYEEGlreGLFCRLPSGEVVRGPVWPGLAAFPDFTDPKARAWWGEKLKGfl-emGVAGFWLDMNE 410
gi 37521104  357 VSILNPAIKADPdLPIFRDGmerKAFVTTPNAQAVIAPVWPGWCAFPDFSDPEVRHWWSEQYRYll-slGIAGFWHDMNE 435
gi 17232823  360 ITIINPGVRASRkNKLFEEGraqDVFCKLPNGKPAIASVWAGLSAFPDFTNPQARHWWSRQYEYll-dlGITGFWHDMNE 438
gi 29833511  368 VSVVDPAVRAEPgNAVYDAGsaqDAFVRDAAGRVVEGVVWPGESVYPDFTHARVRKWWGGLYEErl-aqGFAGFWHDLNE 446
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
2G3M_A       324 PTDFsraieirdvlsslpv------qfrddrlvttfpdnvVHYLRGkr-vkHEKVRNAYPLYEAMATFKGfrtsh-rnei 395
gi 74563758  329 PTVFncdavatksriyalagat-phsltkeelyckaprgaYHVVNGvk-ipHEAVRGLYPYFEAMATYEGllka--gkep 404
gi 48477164  318 PALFndert--------------------------isgsaLHYTNNgf-ikHSKIHNAYSLLEAKATYEAlkei--kdef 368
gi 41033649  336 PTVFgadiegwakirseaaaglkpmplpreelfkrtaagaVHRLDDgkvveHERAHNAYAYYEAMATYEGlara--gkrp 413
gi 70606930  322 PSPLnkkpf---------------------------npraIHRLDDnsqvyHESVHNLYSLFQAMATKPSv--------d 366
gi 159042253 354 PAAFdypnht------------------------vsskviTHRLDDdsrvpHDFLHNAYALYEAMATYDGlvka--grrp 407
gi 55978216  411 PALFaawgep------------------------tlpasaRHALEGqg-gdHRLAHNLYGLLMARASWEGfrkhaperrp 465
gi 37521104  436 PAAFvawgdr------------------------slprptRHSMEGrg-gdHREAHNLYGLLQARAGYESlctfrpevrp 490
gi 17232823  439 PGVFvlwgdps-----------------------lpphatWHSMEGrg-gdHREAHNFYGLLQAEAGYQAlceyqpqrrp 494
gi 29833511  447 PVSFtafgen------------------------tlprsaRHLLEGrg-gdHREAHNVYALGMARAGYEGlrelspqerp 501
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #            #  #                                                           
2G3M_A       396 FILSRAGYAGIQRYAFIWTGDNTPSWDDLKLQLQLVLGLSISGVPFVGCDIGGFqgrn-----faeIDNSMDLLVKYYAL 470
gi 74563758  405 FILSRSGYLGIQKYAALWTGDVPSTWEGLRLTLMAVLGLSASGVPFVGADVGGFa-----------GIGDYELIARWYQA 473
gi 48477164  369 FILSRSGYPGIQRYAAIWTGDNKASDDDLKLQISMIVSMNLSGIMICGCDLGGFf-----------GYSSPELISRYYKA 437
gi 41033649  414 FVLSRAGYAGIQRYAAVWTGDVVASWDGLRAALMAVLGLAASGVHMVGADVSGFa-----------GYSDPELVVRWYQA 482
gi 70606930  367 FVLSRAGYSGIQRYAAIWTGDNTTSWSDLTLQLALTLGLSISGVPYVGCDLGGFi----------gRTTDYLLLYRYFQI 436
gi 159042253 408 FVLSRAGYAGIQRYAAVWTGDNTSNWEHLRLQLQILLGLSISGVTFIGADVGGFakyvpgsggnvlFTLSPELLVRWYEW 487
gi 55978216  466 FLLTRSGHAGVQRYAWTWTGDVESTWEGLRTTLRALLGLSLSGVYFVGSDIGGFs-----------GNPSPELYLRWFQM 534
gi 37521104  491 FIVSRAGWAGLQRYAWTWTGDTESSWGALAMTVAQVLELGLCGIPYSGPDTGGFr-----------GNPTSELYVRWMQL 559
gi 17232823  495 FIVSRSGWAGLQRYAWTWTGDIITSWEGLRQTIPTVLNLGLSGIAYSGSDIGGFk-----------GHPSAELYLRWFQV 563
gi 29833511  502 FIFSRSGWSGMQRYGGAWSGDVTTGWPGLRASLSLVIGLGLCGVPYSGPDVGGFd-----------GSPSPELYLRWFQL 570
                        330       340       350       360       370
                 ....*....|....*....|....*....|....*....|....*....|....*.
Feature 1                 #                                              
2G3M_A       471 ALFFPFYRSHKAtDGIDTEPVFlpDYYKEKVKEIVELRYKFLPYIYSLALEASEKG 526
gi 74563758  474 AAFFPIYRVHRDkGTPDAEITRlpTKYQQMALEAVKMRLRFMPYLRHLAWEAHLTG 529
gi 48477164  438 AMLFPFFRNHKVkEGNDQEIYLlpEKYRNEIIETVNERYKFIDYIYSIIKLSSLTG 493
gi 41033649  483 SLFFPLFRQHKGrEGNDVEFFAlpAKYREAVIEAIKLRYRFLPYLWHLAWEAHLTG 538
gi 70606930  437 ALFFPIFRNHKDkGGSDQEIYSipDYWKEKIKRVIKMRYQFLPYLNALARESSKTG 492
gi 159042253 488 AIFFPLLRNHASiGSPDQEPWAfgPRTLELIKNLLRLRARLTPYLYSLMWLSHING 543
gi 55978216  535 AALTPFFRLHAArWTKRREPWRfgEEVLEGVRRAMALRESLLPYLYTLAHRASREG 590
gi 37521104  560 SAFLPFFRTHASnDARSRAPWTfgEPSLGIARAFIKLRYRLLPYLYTLCWEASRRG 615
gi 17232823  564 SCFMPFCRTHSAnNTKPRTPWSfgEPTLGIVRQFLQLRYRLMPYFYTLAWESTQTG 619
gi 29833511  571 GSYLPLFRTRAGlRAGRRELWEfgEDVLEHARVALVERRRLLPYFMTLAHLARRTG 626

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