1F3M,2HY8,1YHV


Conserved Protein Domain Family
STKc_PAK_I

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cd06647: STKc_PAK_I (this model, PSSM-Id:132978 is obsolete and has been replaced by 270814)
Click on image for an interactive view with Cn3D
Catalytic domain of the Protein Serine/Threonine Kinase, Group I p21-activated kinase
Serine/threonine kinases (STKs), p21-activated kinase (PAK) subfamily, Group I, catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The PAK subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. PAKs are Rho family GTPase-regulated kinases that serve as important mediators in the function of Cdc42 (cell division cycle 42) and Rac. PAKs are implicated in the regulation of many cellular processes including growth factor receptor-mediated proliferation, cell polarity, cell motility, cell death and survival, and actin cytoskeleton organization. PAKs from higher eukaryotes are classified into two groups (I and II), according to their biochemical and structural features. Group I PAKs, also called conventional PAKs, include PAK1, PAK2, and PAK3. Group I PAKs contain a PBD (p21-binding domain) overlapping with an AID (autoinhibitory domain), a C-terminal catalytic domain, SH3 binding sites and a non-classical SH3 binding site for PIX (PAK-interacting exchange factor). They interact with the SH3 domain containing proteins Nck, Grb2 and PIX. Binding of group I PAKs to activated GTPases leads to conformational changes that destabilize the AID, allowing autophosphorylation and full activation of the kinase domain. Known group I PAK substrates include MLCK, Bad, Raf, MEK1, LIMK, Merlin, Vimentin, Myc, Stat5a, and Aurora A, among others.
Statistics
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PSSM-Id: 132978
View PSSM: cd06647
Aligned: 9 rows
Threshold Bit Score: 586.871
Threshold Setting Gi: 51701750
Created: 3-Sep-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 36 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:The active site is composed of the ATP binding site and the substrate binding site. The substrate binding site is based on the binding of human PAK4 to a consensus peptide.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                  #####   #            # #                            # 
1F3M_C         2 DEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIRQMNLQQQPKKELIINEILVMRENkNPNIVN 81  human
2HY8_1         2 DEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIRQMNLQQQPKKELIINEILVMRENkNPNIVN 81  human
1YHV_A         2 DEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIRQMNLQQQPKKELIINEILVMRENkNPNIVN 81  human
Q17850       275 DAEVLTKLRTIVSIGNPDRKYRKVDKIGSGASGSVYTAIEISTEAEVAIKQMNLKDQPKKELIINEILVMRENkHANIVN 354 nematode
AAQ23609     410 DEEILEKLRTIVSVGDPNRKYTKMEKIGQGASGTVYTAIESSTGMEVAIKQMNLSQQPKKELIINEILVMRENkHPNVVN 489 fruit fly
XP_001625767 206 DEEILARLRTIVSIGDPKKKYTKFEKIGQGASGTVYTAIEVATGHEVAIKQMNLSQQPKKELIINEILVMRENkHPNIVN 285 starlet sea ane...
NP_001081929 232 DEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYIAVDVATGQEVAIKQMNLQQQPKKELIINEILVMRENkHPNIVN 311 African clawed ...
Q64303       229 DEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVFTATDVALGQEVAIKQINLQKQPKKELIINEILVMKELkNPNIVN 308 Norway rat
CAI43167     248 DEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTALDIATGQEVAIKQMNLQQQPKKELIINEILVMRENkNPNIVN 327 human
Feature 1                      ##### ##  #                                  # #### #         ##  
1F3M_C        82 YLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGmDGSVKLTDFG 161 human
2HY8_1        82 YLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGmDGSVKLTDFG 161 human
1YHV_A        82 YLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGmDGSVKLTDFG 161 human
Q17850       355 YLDSYLVCDELWVVMEYLAGGSLTDVVTECQMEDGIIAAVCREVLQALEFLHSRHVIHRDIKSDNILLGmDGSVKLTDFG 434 nematode
AAQ23609     490 YLDSYLVSEELWVVMEYLPGGSLTDVVTETCMDEGQIAAVCREVLQALEFLHANQVIHRDIKSDNILLGlDGSVKLTDFG 569 fruit fly
XP_001625767 286 YVDSYLVGEELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNGVIHRDIKSDNILLGmDGQVKLTDFG 365 starlet sea ane...
NP_001081929 312 YLDSYLVGEELWVVMEYLAGGSLTDVVTETCLQEGEIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGmDGAVKLTDFG 391 African clawed ...
Q64303       309 FLDSYLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGmEGSVKLTDFG 388 Norway rat
CAI43167     328 YLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGmDGSVKLTDFG 407 human
Feature 1        #            ##### #                          #        ###                      
1F3M_C       162 FCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPek 241 human
2HY8_1       162 FCAQITPEQSKRSEMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPek 241 human
1YHV_A       162 FCAQITPEQSKRSEMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPek 241 human
Q17850       435 FCAQLSPEQRKRTTMVGTPYWMAPEVVTRKQYGPKVDVWSLGIMAIEMVEGEPPYLNENPLRAIYLIATNGKPDFPGRds 514 nematode
AAQ23609     570 FCAQISPEQSKRTTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMAIEMVEGEPPYLNENPLKALYLIATNGKPEIKEKdk 649 fruit fly
XP_001625767 366 FCATITPEQNKRSTMVGTPYWMAPEVVTRKQYGPRVDVWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELAHPek 445 starlet sea ane...
NP_001081929 392 FCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDVWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQQPek 471 African clawed ...
Q64303       389 FCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPek 468 Norway rat
CAI43167     408 FCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPer 487 human
Feature 1                                                             
1F3M_C       242 LSAIFRDFLNRCLDMdVEKRGSAKELLQHQFLKiAKPLSSLTPLIAAAKEATK 294 human
2HY8_1       242 LSAIFRDFLNRCLDMdVEKRGSAKELLQHQFLKiAKPLSSLTPLIAAAKEATK 294 human
1YHV_A       242 LSAIFRDFLNRCLDMdVEKRGSAKELLQHQFLKiAKPLSSLTPLIAAAKEATK 294 human
Q17850       515 MTLLFKDFVDSALEVqVENRWSASQLLTHPFLRcAKPLASLYYLIVAAKKSIA 567 nematode
AAQ23609     650 LSSAFQDFLDQCLEVeVDRRASALDLLKHPFLKlARPLASLTPLIMAAKEATK 702 fruit fly
XP_001625767 446 LSPVFKDFLAQSLEMdVEKRSSARELLQHPFLKqAKPLASLVPLILAAKEATS 498 starlet sea anemone
NP_001081929 472 QSSAFRDFLSRCLEMdVEKRVSARDLLQHPFLKiAKPLSSLTPYIITGKQIAK 524 African clawed frog
Q64303       469 LSPIFRDFLNRCLEMdVEKRGSAKELLQHPFLKlAKPLSSLTPLILAAKEAMK 521 Norway rat
CAI43167     488 LSAVFRDFLNRCLEMdVDRRGSAKELLQHPFLKlAKPLSSLTPLIIAAKEAIK 540 human

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