Conserved Protein Domain Family
PKc_PBS2_like

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cd06622: PKc_PBS2_like 
Catalytic domain of fungal PBS2-like dual-specificity Mitogen-Activated Protein Kinase Kinases
PKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine (ST) or tyrosine residues on protein substrates. Members of this group include the MAPKKs Polymyxin B resistance protein 2 (PBS2) from Saccharomyces cerevisiae, Wis1 from Schizosaccharomyces pombe, and related proteins. PBS2 and Wis1 are components of stress-activated MAPK cascades in budding and fission yeast, respectively. PBS2 is the specific activator of the MAPK Hog1, which plays a central role in the response of budding yeast to stress including exposure to arsenite and hyperosmotic environments. Wis1 phosphorylates and activates the MAPK Sty1 (also called Spc1 or Phh1), which stimulates a transcriptional response to a wide range of cellular insults through the bZip transcription factors Atf1, Pcr1, and Pap1. The PBS2 subfamily is part of a larger superfamily that includes the catalytic domains of STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.
Statistics
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PSSM-Id: 132953
View PSSM: cd06622
Aligned: 4 rows
Threshold Bit Score: 513.242
Threshold Setting Gi: 28189093
Created: 16-Nov-2007
Updated: 2-Mar-2014
Structure
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Aligned Rows:
 
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               #####   #            # #                             #               ###
gi 83305209 358 DELEFLDELGHGNYGNVSKVLHkptnVIMATKEVRLELDeakFRQILMELEVLHKCnSPYIVDFYGAFFIegAVYMCMEY 437
gi 28189093 289 DEVEVMDELGKGNYGTVYKVRHs---KVMAMKEIRLELDeskFSTILKELVILHECaSPYIIDFYGAFYQegAVYMCIEY 365
gi 465483   318 SEIIKLEELGKGNYGVVYKALHqptgVTMALKEIRLSLEeatFNQIIMELDILHKAvSPYIVDFYGAFFVegSVFICMEY 397
gi 57223234 293 DEIEVLGELGKGNYGSVHKVFHrptgVTMAMKEIRLELDdskLNGIIMELDILHRAvAPEIVEFYGAFTIesCVYYCMEY 372
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1       #  ##  #                                                   # #### #          ## 
gi 83305209 438 MdgGSLDKIYdesse------------iggiDEPQLAFIANAVIHGLKELKEqHNIIHRDVKPTNILCSanqGTVKLCDF 505
gi 28189093 366 MdgGSIDKLYag-----------------giPENVLRKITYATIMGLKCLKEdHNIIHRDVKPTNILVNt-nGQVKICDF 427
gi 465483   398 MdaGSMDKLYagg----------------ikDEGVLARTAYAVVQGLKTLKEeHNIIHRDVKPTNVLVNs-nGQVKLCDF 460
gi 57223234 373 MdaGSLDSLTgggvaakdqtkeeeddatervPEDVLRRITARIVKGLRFLKDeLQIIHRDVKPTNVLINg-kGEVKMCDF 451
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        #           #### #                                     #        #              
gi 83305209 506 GVSGNLVASLAKTNIGCQSYMAPERIKSlnp------draTYTVQSDIWSLGLSILEMAlgRYPYPPEtydnIFSQLSAI 579
gi 28189093 428 GVSGNLVASIAKTNIGCQSYMAPERISGggmsaaggaaagTYSVQSDIWSLGLTIIECAmgRYPYPPEvsstIFSQLSAI 507
gi 465483   461 GVSGNLVASISKTNIGCQSYMAPERIRVggpt----ngvlTYTVQADVWSLGLTILEMAlgAYPYPPEsytsIFAQLSAI 536
gi 57223234 452 GVSGQLEKSLAKTNIGCQSYMAPERIKSetan-----qnpTYTVSSDVWSVGLSIVELAkgCYPYPPEtyanVFAQLQAI 526
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                       
gi 83305209 580 VdgPPPRLPSDkFSSDAQDFVSLCLQKiPERRPTyAALTEHPWLVKYRNq------------------------------ 629
gi 28189093 508 VegDPPGLPSEgYSGTAQDFVKSCLNKiPAKRHTyPMLLMHPWIKSLGRpetiteeveaeekaaddqladatgsldinsn 587
gi 465483   537 CdgDPPSLPDS-FSPEARDFVNKCLNKnPSLRPDyHELANHPWLLKYQNa------------------------------ 585
gi 57223234 527 VhgTPPTLPPG-YSDDANDFVAKCLEKdPNRRPTyAQLLEHPFLVADKGa------------------------------ 575
                       330       340
                ....*....|....*....|..
Feature 1                             
gi 83305209 630 -------dVHMSEYITERLERR 644
gi 28189093 588 gpindqgdREVAEWVTNVLDRK 609
gi 465483   586 -------dVDMASWAKGALKEK 600
gi 57223234 576 -------eVDMVGWVEGALKRK 590

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