3CKX


Conserved Protein Domain Family
STKc_MST3_like

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cd06609: STKc_MST3_like (this model, PSSM-Id:132940 is obsolete and has been replaced by 270786)
Click on image for an interactive view with Cn3D
Catalytic domain of Mammalian Ste20-like protein kinase 3-like Protein Serine/Threonine Kinases
Serine/threonine kinases (STKs), mammalian Ste20-like protein kinase 3 (MST3)-like subfamily, catalytic (c) domain. STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The MST3-like subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase. This subfamily is composed of MST3, MST4, STK25, Schizosaccharomyces pombe Nak1 and Sid1, Saccharomyces cerevisiae sporulation-specific protein 1 (SPS1), and related proteins. Nak1 is required by fission yeast for polarizing the tips of actin cytoskeleton and is involved in cell growth, cell separation, cell morphology and cell-cycle progression. Sid1 is a component in the septation initiation network (SIN) signaling pathway, and plays a role in cytokinesis. SPS1 plays a role in regulating proteins required for spore wall formation. MST4 plays a role in mitogen-activated protein kinase (MAPK) signaling during cytoskeletal rearrangement, morphogenesis, and apoptosis. MST3 phosphorylates the STK NDR and may play a role in cell cycle progression and cell morphology. STK25 may play a role in the regulation of cell migration and polarization.
Statistics
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PSSM-Id: 132940
View PSSM: cd06609
Aligned: 24 rows
Threshold Bit Score: 438.589
Threshold Setting Gi: 68487165
Created: 2-Aug-2006
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 32 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:The active site is composed of the ATP binding site and the substrate binding site. The substrate binding site is based on the binding of human PAK4 to a consensus peptide.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                 #####   #                       # #                #             #      
3CKX_A          7 ELFTKLEKIGKGSFGEVFKGIDnr-----------tqKVVAIKIIDLEeaeDEIEDIQQEITVLSQCd-SPYVTKYYGSY 74
gi 1174431     16 KLYSIQSCIGRGNFGDVYKAVDrv-----------tqEIVAIKVVNLEhsdEDIELLAQEIFFLAELk-SPLITNYIATM 83
gi 729897      21 SLFKRTEVIGRGKFGVVYKGYNvk-----------tgRVYAIKVLNLDsdsDEVEDVQREIQFLASLkqISNITRYYGSY 89
gi 50304565     5 QQFQLLECVGKGNFGDVYLAHYlgsnqeptdpkipknVPLAIKCINLEhsnEPIDLLLKEIYFLSTLh-CPYITHYYGTF 83
gi 50551119    56 DQFELLEELGSGSFGVVYKAIDkv-----------sgQIVAVKKIDLEsseDDIEEIQKEIAILSGCq-DEHITTYYGCF 123
gi 31077007     7 NSYTLLRKLGSGSFGVVWKAREnv-----------sgDIIAIKQIDLEtgiDDITDIEQEVFMLSNCn-SSNVIQYYGCF 74
gi 50422805     4 DKYKIHQCIGKGNFGDVYKATDis-----------nnTLVAVKVINLDesdEDIAVLIQEIQFLSKLr-SPYITKYFETF 71
gi 2414625      4 HQYRDLQLIGQGSFGSVFRAQDve-----------ssKIVALKVVDLDatkDQIETLTQEINFLIDLn-SVHITKYYASF 71
gi 68487165    10 EELEVYEEVGRGGFGVVYRGIIks-----------tnQEVAIKQIDLEkdsTDLIEINKEIQIISECh-CQQITSYMGSF 77
gi 146416649    3 NKYCLEKCIGRGNFGDVYKAACkr-----------sgKVVAVKVVNLDeslEDMFFMVQEIQFLSLMr-SPYIVQYLESF 70
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                  ####  ##  #                                     # #### #           #  #
3CKX_A         75 LkdtkLWIIMEYLgGGSALDLLEpg---pLDETQIATILREILKGLDYLHSEKKIHRDIKAANVLLSe-hGEVKLADFGV 150
gi 1174431     84 LedvsMWIVMEYCgGGSCSDLLKrsyvngLPEEKVSFIIHEVTLGLKYLHEQRKIHRDIKAANILLNe-eGMVKLGDFGV 162
gi 729897      90 LkdtsLWIIMEHCaGGSLRSLLRpg---kIDEKYIGVIMRELLVALKCIHKDNVIHRDIKAANVLITn-eGNVKLCDFGV 165
gi 50304565    84 TgdcnLYIVMEYCsNGSLLNLLRyy--srLTEQTTCFIILQVCHALEYLHEKRLIHRDLKAANILLNd-dGEVRLADLGV 160
gi 50551119   124 VrgykLWIIMEYLaGGSGLDLLKpg---iFHEPEIAVMCRELLEGLIYLHDNGKIHRDVKAANVLVSs-eGSVKLADFGV 199
gi 31077007    75 VdgytLWILMEHMdGGSVSGLLKmg---rLNEQVISIILREVLYGLNYLHGQNKIHRDIKAANILLSsstGNVKLADFGV 151
gi 50422805    72 IndmsMWIVMEYCgGGSCADLLKcy--rkLNEETTAFIIRDVLRGLTYLHEENKVHRDIKLANILLTs-yGEIKLADFGV 148
gi 2414625     72 VdgfrLWITMEYCdGGSCLDLLKls--gtFSERVIAEVMRQVLEALVYLHGQGKMHRDIKAANILTMk-dGLVKLADFGV 148
gi 68487165    78 VknykLWVIMEFIdGGSIFELLKpg--piDDEKIVSFILYEILLALNYLHNQGKIHRDLKSQNILISk-tGDIKLTDFGV 154
gi 146416649   71 VensnMWIIMEYCgGGSVADVLRyc--kkFDENIAAYIIKYVLLGLSYLHKQKTVHRDVKLANVLVTt-dGTIKLADFGV 147
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                           #### #                            #        #                  
3CKX_A        151 AGQLTDTq-----IKRNXFVGTPFWMAPEVIKQSa--YDSKADIWSLGITAIELArGEPPHSELh-PMKVLFLIPKn--- 219
gi 1174431    163 SGHIRST------LKRDTFVGTPYWMAPEVVCCEvdgYNEKADIWSLGITTYELLkGLPPLSKYd-PMKVMTNLPKr--- 232
gi 729897     166 AAQVNQTs-----LRRQTMAGTPYWMAPEVIMEGv-yYDTKVDIWSLGITTYEIAtGNPPYCDVe-ALRAMQLIIKs--- 235
gi 50304565   161 TGQLKFNstrhggKNLNTFVGTPFWMAPEIIKNQs--YDGKCDIWSLGITTLELLnGKPPMSHLd-SMKALMRIPKl--- 234
gi 50551119   200 ATQLSNNm-----SRRNTFVGTPFWMAPEVIRQEd--YDTKADIWSLGITAIEFAkGEPPLSEYh-PMKVLFLIPKa--- 268
gi 31077007   152 AAQLSNAa-----SRRHTFVGTPFWMAPEVIQQTs--YGLAADIWSLGITAIEMAnGIPPRATMh-PMRVIFEIPQs--- 220
gi 50422805   149 SGEITMTq-----LKRNTFVGTPFWMAPEVITRLktgYNEKADIWSTGITTIELVtGSPPLSQYd-PMKILFEIPKk--- 219
gi 2414625    149 SGQLESLr-----DKNDDFVGTPFWMAPEVVKQTg--YNYKADIWSLGITAYELAtGEPPYSGIh-PMKVLLLIPKh--- 217
gi 68487165   155 STQLSSNf-----SKRNTTVGTPYWMAPEVIVNNnggHSYKADIWSLGCCCYEMFtGKPPLQSKypPMRALRLISKcqkn 229
gi 146416649  148 SGEMSFTt-----NKRSTFVGTPYWMAPEVIVHSklgYNEKADIWSTGITTIELIkGDPPLADYd-PMKILFKIPKq--- 218
                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
Feature 1                                                                           
3CKX_A        220 --NPPTLEGN---YSKPLKEFVEACLNKePSFRPTAKELLKHKFILRnakktsyltELIDRYKRWK 280
gi 1174431    233 --KPPKLQGP---FSDAAKDFVAGCLVKtPADRPSAYNLLSFEFVKNitit--nlkSDVDLIKQKK 291
gi 729897     236 --KPPRLEDRs--YSTSLKEFIALCLDEdPKERLSADDLLKSKFIRAhkat--ptsILKELISRYL 295
gi 50304565   235 --NADSILRNm-dISPLGKDFIRSCLQQdPNQRPTCKQLLQHKWLKKcs-----itNIMEEITVMK 292
gi 50551119   269 --EPPTVPAGg-nWSADFRDFVACCLRKnPAERPSGRQLLKHRFIRKagkt-sqlkLLVDRRERWM 330
gi 31077007   221 --EPPKLDDH---FSPTFRDFVSCCLDLnPNMRWSAKELLQHPFIKSagtvkdiipLLVQKENKLF 281
gi 50422805   220 --RPPLLTGVd--FSENIKDFVRYCLIKdPKKRPSSSTLLHHKFLKNikrn-vnlfKLLEEKNKWS 280
gi 2414625    218 --SPPSLERSk--FSRAFCDFVSNCLKKnPKDRATAEYLSKHKFIKKycpn-tsvkEVVASYAKWK 278
gi 68487165   230 qdFIKLIELNeldISWEFKDFLHQCFIEdPKLRASASKLLKHKFITKhihdkdkikLLKKLITKKQ 295
gi 146416649  219 --EPPLLTGDk--YSDELKGFLEKCLVKnPRKRSSSTELLDHPFIGIaaeqqdfckLLRRRRTSFL 280

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