Conserved Protein Domain Family
PBP1_LacI

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cd01574: PBP1_LacI 
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Ligand-binding domain of DNA transcription repressor LacI specific for lactose, a member of the LacI-GalR family of bacterial transcription regulators
Ligand-binding domain of DNA transcription repressor LacI specific for lactose, a member of the LacI-GalR family of bacterial transcription regulators. The ligand-binding domain of LacI is structurally homologous to the periplasmic sugar-binding domain of ABC-type transporters and both domains contain the type I periplasmic binding protein-like fold. The LacI-GalR family repressors are composed of two functional domains: an N-terminal HTH (helix-turn-helix) domain, which is responsible for the DNA-binding specificity, and a C-terminal ligand-binding domain, which is homologous to the type I periplasmic binding proteins. As also observed in the periplasmic binding proteins, the C-terminal domain of the bacterial transcription repressor undergoes a conformational change upon ligand binding which in turn changes the DNA binding affinity of the repressor.
Links
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Statistics
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PSSM-Id: 107259
View PSSM: cd01574
Aligned: 46 rows
Threshold Bit Score: 215.523
Threshold Setting Gi: 148656431
Created: 21-Jul-2004
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
Hierarchy
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Display:
 
Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:ligand binding site [chemical binding site]
Evidence:
  • Structure:1JWL_A; Escherichia coli LacI bound to ONPF anti-inducer, contacts calculated at 3.5 A.
    View structure with Cn3D
  • Structure:2P9H_A; Escherichia coli LacI bound to IPTG inducer, contacts calculated at 3.5 A.
    View structure with Cn3D

cd01574 is part of a hierarchy of related CD models.
Use the graphical representation to navigate this hierarchy.
cd01574 is a member of the superfamily cl10011.
cd01391:Periplasmic_Binding_Protein_Type_1cd01536:PBP1_ABC_sugar_binding_likecd01537:PBP1_Repressors_Sugar_Binding_likecd01538:PBP1_ABC_xylose_bindingcd01539:PBP1_GGBPcd01540:PBP1_arabinose_bindingcd01541:PBP1_AraRcd01542:PBP1_TreR_likecd01543:PBP1_XylRcd01544:PBP1_GalRcd01545:PBP1_SalRcd01574:PBP1_LacIcd01575:PBP1_GntRcd04509:PBP1_ABC_transporter_GCPR_C_likecd06267:PBP1_LacI_sugar_binding_likecd06268:PBP1_ABC_transporter_LIVBP_likecd06269:PBP1_glutamate_receptors_likecd06270:PBP1_GalS_likecd06271:PBP1_AglR_RafR_likecd06272:PBP1_hexuronate_repressor_likecd06273:PBP1_GntR_like_1cd06274:PBP1_FruRcd06275:PBP1_PurRcd06276:PBP1_FucR_likecd06277:PBP1_LacI_like_1cd06278:PBP1_LacI_like_2cd06279:PBP1_LacI_like_3cd06280:PBP1_LacI_like_4cd06281:PBP1_LacI_like_5cd06282:PBP1_GntR_like_2cd06283:PBP1_RegR_EndR_KdgR_likecd06284:PBP1_LacI_like_6cd06285:PBP1_LacI_like_7cd06286:PBP1_CcpB_likecd06287:PBP1_LacI_like_8cd06288:PBP1_sucrose_transcription_regulatorcd06289:PBP1_MalI_likecd06290:PBP1_LacI_like_9cd06291:PBP1_Qymf_likecd06292:PBP1_LacI_like_10cd06293:PBP1_LacI_like_11cd06294:PBP1_ycjW_transcription_regulator_likecd06295:PBP1_CelRcd06296:PBP1_CatR_likecd06297:PBP1_LacI_like_12cd06298:PBP1_CcpA_likecd06299:PBP1_LacI_like_13cd06300:PBP1_ABC_sugar_binding_like_1cd06301:PBP1_rhizopine_binding_likecd06302:PBP1_LsrB_Quorum_Sensingcd06303:PBP1_LuxPQ_Quorum_Sensingcd06304:PBP1_BmpA_likecd06305:PBP1_methylthioribose_binding_likecd06306:PBP1_TorT-likecd06307:PBP1_uncharacterized_sugar_bindingcd06308:PBP1_sensor_kinase_likecd06309:PBP1_YtfQ_likecd06310:PBP1_ABC_sugar_binding_like_2cd06311:PBP1_ABC_sugar_binding_like_3cd06312:PBP1_ABC_sugar_binding_like_4cd06313:PBP1_ABC_sugar_binding_like_5cd06314:PBP1_tmGBPcd06315:PBP1_ABC_sugar_binding_like_6cd06316:PBP1_ABC_sugar_binding_like_7cd06317:PBP1_ABC_sugar_binding_like_8cd06318:PBP1_ABC_sugar_binding_like_9cd06319:PBP1_ABC_sugar_binding_like_10cd06320:PBP1_allose_bindingcd06321:PBP1_ABC_sugar_binding_like_11cd06322:PBP1_ABC_sugar_binding_like_12cd06323:PBP1_ribose_bindingcd06324:PBP1_ABC_sugar_binding_like_13cd06325:PBP1_ABC_uncharacterized_transportercd06326:PBP1_STKc_likecd06327:PBP1_SBP_like_1cd06328:PBP1_SBP_like_2cd06329:PBP1_SBP_like_3cd06330:PBP1_Arsenic_SBP_likecd06331:PBP1_AmiC_likecd06332:PBP1_aromatic_compounds_likecd06333:PBP1_ABC-type_HAAT_likecd06334:PBP1_ABC_ligand_binding_like_1cd06335:PBP1_ABC_ligand_binding_like_2cd06336:PBP1_ABC_ligand_binding_like_3cd06337:PBP1_ABC_ligand_binding_like_4cd06338:PBP1_ABC_ligand_binding_like_5cd06339:PBP1_YraM_LppC_lipoprotein_likecd06340:PBP1_ABC_ligand_binding_like_6cd06341:PBP1_ABC_ligand_binding_like_7cd06342:PBP1_ABC_LIVBP_likecd06343:PBP1_ABC_ligand_binding_like_8cd06344:PBP1_ABC_ligand_binding_like_9cd06345:PBP1_ABC_ligand_binding_like_10cd06346:PBP1_ABC_ligand_binding_like_11cd06347:PBP1_ABC_ligand_binding_like_12cd06348:PBP1_ABC_ligand_binding_like_13cd06349:PBP1_ABC_ligand_binding_like_14cd06350:PBP1_GPCR_family_C_likecd06351:PBP1_iGluR_N_LIVBP_likecd06352:PBP1_NPR_GC_likecd06353:PBP1_BmpA_Med_likecd06354:PBP1_BmpA_PnrA_likecd06355:PBP1_FmdD_likecd06356:PBP1_Amide_Urea_BP_likecd06357:PBP1_AmiCcd06358:PBP1_NHasecd06359:PBP1_Nba_likecd06360:PBP1_alkylbenzenes_likecd06361:PBP1_GPC6A_likecd06362:PBP1_mGluRcd06363:PBP1_Taste_receptorcd06364:PBP1_CaSRcd06365:PBP1_Pheromone_receptorcd06366:PBP1_GABAb_receptorcd06367:PBP1_iGluR_NMDAcd06368:PBP1_iGluR_non_NMDA_likecd06369:PBP1_GC_C_enterotoxin_receptorcd06370:PBP1_Speract_GC_likecd06371:PBP1_sensory_GC_DEF_likecd06372:PBP1_GC_G_likecd06373:PBP1_NPR_likecd06374:PBP1_mGluR_groupIcd06375:PBP1_mGluR_groupIIcd06376:PBP1_mGluR_groupIIIcd06377:PBP1_iGluR_NMDA_NR3cd06378:PBP1_iGluR_NMDA_NR2cd06379:PBP1_iGluR_NMDA_NR1cd06380:PBP1_iGluR_AMPAcd06381:PBP1_iGluR_delta_likecd06382:PBP1_iGluR_Kainatecd06383:PBP1_iGluR_AMPA_Likecd06384:PBP1_NPR_Bcd06385:PBP1_NPR_Acd06386:PBP1_NPR_C_likecd06387:PBP1_iGluR_AMPA_GluR3cd06388:PBP1_iGluR_AMPA_GluR4cd06389:PBP1_iGluR_AMPA_GluR2cd06390:PBP1_iGluR_AMPA_GluR1cd06391:PBP1_iGluR_delta_2cd06392:PBP1_iGluR_delta_1cd06393:PBP1_iGluR_Kainate_GluR5_7cd06394:PBP1_iGluR_Kainate_KA1_21JWL A1TLF A1LBI A2P9H A2PE5 A146310588779757584961095515693512712593316121157148656431119945696843872319040727650841230508396915084208923464877154488014508411012346541314827388021225681139438373139438388139438376139438370234657371166704741184723871197146441545076081341008811153796801179280781583164456814649914827205415296689111996418721224662508391811482712141166704671482716041JWL A1TLF A1LBI A2P9H A2PE5 A14631058877975758496109551569351271259331612115714865643111994569684387231904072765084123050839691508420892346487715448801450841101234654131482738802122568113943837313943838813943837613943837023465737116670474118472387119714644154507608134100881115379680117928078158316445681464991482720541529668911199641872122466250839181148271214116670467148271604
cd01574 Sequence Cluster
cd01574 Sequence Cluster
Sub-family Hierarchy
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 cd01574 Branch
 Whole Hierarchy
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CD Hierarchy
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1              #     ##  #                                             #                 
1JWL_A        63 LIGVATSSLAlHAPSQIVAAIKSRAdqlGASVVVSMversgveacktAVHNLLAQRVSGLIINyplddqda--iaveaac 140
gi 148656431  10 TLGCVLNDPRfTFWSILRHGIRSRAa--DHGIAVIDqaagaveaqveMVADLLRRRVHALILGpahetrafaqhlelarl 87
gi 119945696  62 MIGLVTLDILlHGASLLANGVRKQAknlGYGTSLSViadpaidkittAVDDLKAQRVEFIIMNiplssdna--emlvvqy 139
gi 77975758   65 TLGLATTDLSlHAPSQIAAAIKRKArelGYNVVIAMvddhsehacqqAINELLAQRVDGILVNvplkpkrs--qkiaqqc 142
gi 146310588  63 TLGLITTDLAlHAPSQIASAVKSRAgerGASVMISMveqpe--fcqaALQELLAQRVEGLLVNvplddaea--ekirhla 138
gi 84387231   62 SVGLVTLDMAlHGAAMIANGVRMQAhdmSYGVAFSVvsepnlantreAIRELMAQQVDSIILNvplesada--ellveqy 139
gi 49610955   65 TLGLVTTDLSlHAPSQIAAAIKSTAsqlGINIVMSMlsspdvntcnnAVNDLLSQRVSGVIVNvplstdev--ahisqtc 142
gi 156935127  94 TLGFITVSLAlHAPSQIAAAIKSHAaraDYSVVIATletgtleelqrSLNEMRAQKVDGVIINlplgaeaa--aslvndn 171
gi 125933     65 SIGLITASVTlHAPSQIAAAIKSHAslhQLEVAIAMpaqadfvalqaRLDELRAQHIRGVIVSlplesata--erlvqdn 142
gi 16121157   65 TMGLVTTSLAlHAPSQVAAAIKRYAhieGYQVLISMidesvnqsiqhSINDLKSQWVGKVIINvpletava--ekiaadn 142
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                #             #                                 #   #                   
1JWL_A       141 TNVPALFLDvsdq--tpinSIIFSHEDGTRLGVEHLVa--lGHQQIALLAGPlssvSARLRLAGWHKYlt-rnQIQPIAE 215
gi 148656431  88 ARVPVIEVDggilegyvsaTVRSNERQGLMRVAELLVahlgDRMRIALIAGPrn-rRAEIMAEVLRQWp----GVQIVAQ 162
gi 119945696 140 SSIHFVFIDvppd--sqvnSVSGASYNGAKIAAELMLs--lCRKRLLFINGPqgssASRLRLQGWLDVlkedaDSKIINQ 215
gi 77975758  143 VDTPALFLDvdpq--saafSVLFDPKLGAIQGAEHLIa--lGHQQIVFICGPketiSAQLRCQGWLQAls-taSLVPQAV 217
gi 146310588 139 APVPVLFFDvsps--anvhRLAFDAEQGATLGAEHLLs--lGHQHIALLAGPessvSSRARLSGWQARlg-qaNVTPCAI 213
gi 84387231  140 QHLNLIFIDvpsn--sqvnYVCGDHAEGAKLAAQHLLe--sGRTDFLLITGPnessASKIRHQSWLAAls-daESKVQYQ 214
gi 49610955  143 ADTPVLFMDadph--tdilNVMFDPDHGARLAIAHLVe--lGHQHIALLTGPmtsiSARLRYEGWLAEle-kqRLSPVSV 217
gi 156935127 172 SDLLCLFLDvpad--sdvfHVSFDPRDGSRQSVELLLn--gGHQRIALLAGPehsvASGMRLACWQEVls-ahGLAPCAV 246
gi 125933    143 PDMACLFLDvspe--advcCVRFDHRDGCGACVRHLWe--mGHREFGLLAGPessvSARLRLASWREAlh-slNIARSTT 217
gi 16121157  143 DDVTCLFLDvdpy--ssvfNVSFNPADGTRASVKYLYe--lGHREIALLAGPkqsvSANLRLKSWLETla-dyGLSPVSV 217
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                      #                           #                     
1JWL_A       216 REGDWSAMSGFQQTMQMLNegivptAMLVANDQMALGAMRAITESGlrVGADISVVGYDDTedss---cyiPPLTTIKQD 292
gi 148656431 163 STGDWTRDSGRRLAEEWLRtgqpidAIFAANDPMALGVVDAVEALN--LTGSIAVVGFDGLpealrainsgAMLATVNQD 240
gi 119945696 216 FEGDWRASSGYRCMRQALSdghdfdGVLVANDQMALGVLRALSEFNlsVPQQVSVIGFDDTvdsa---yftPPLTTIKQD 292
gi 77975758  218 YYGEWNAQSGYQATQKLLIsntpfsAILVANDQMALGVLRALHEHNidVPGQVSVIGYDDTadsa---yfqPPLTTIRQN 294
gi 146310588 214 AHGDWSAASGYEKGHQLLSgatlpdAILVANDQMALGVMRACSEKGiaVPGQISVVGFDDTadsa---wfsPPLTTIRQA 290
gi 84387231  215 YQGNWQAESGYLGVREAVAkqavfdAVLVASDQMALGALRALQELQipVPDKVAVVGFDGIedsa---ffnPPLTTIKQD 291
gi 49610955  218 LHGDWSAASGYHQTLALLGqslrisAIVVANDQMALGVLRALHEYGlrVPEQMSVIGFDDTqdsa---yytPPLTTIRQD 294
gi 156935127 247 LHGDWSSKDAWQQTLSLFRtrrditALVVANDQMAFGAMSALDELGlrVPQDVSVIGYDDTpdsa---yfiPPLTTVEQD 323
gi 125933    218 VFGDWSAASGWQKTFELLHlqprisAIVVANDQMALGVLSALAQLNrsGSQAVSVTGYDDTadsl---yfqPPLTTVAQD 294
gi 16121157  218 IHGNWDAQSGYSGALQMLRetpqfsAVLVANDQMALGVLSAFHQNQlaVPGEKSVIGYDDTyess---ffyPALTTVSLD 294
                        250       260       270
                 ....*....|....*....|....*....|....*...
Feature 1                                              
1JWL_A       293 FRLLGQTSVDRllqlsq--gqavkGNQLLPVSLVKRKT 328
gi 148656431 241 PLCIGQQAVDTalqalr--sepveRQILTPGELITRDT 276
gi 119945696 293 FLTIGERAVLLafsvad-kakksyRHESIVTELIERKS 329
gi 77975758  295 FKLLGEESVSRlivrlhhtieppsESLLLATELTIRQT 332
gi 146310588 291 FRDAGERSVEWllssa----tnelWQTQLPVTLVARHS 324
gi 84387231  292 FTTIGRQAVILaeklna-nsqdtlLQHHIETTLLPRES 328
gi 49610955  295 FRLLGKEGVTRllatlr--dptqaTSLLLPTELIVRHS 330
gi 156935127 324 FNRLGAQAVSRmvarlt--ghgesGSTLLPTRFIERRS 359
gi 125933    295 FDLLGKRAVERlialma--apqlrIRELLPTRLIVRQS 330
gi 16121157  295 LDLQGKEAVRRmlsasd--nesmrSSSILPAKLVVRNS 330

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