Conserved Protein Domain Family
beta_tubulin

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cd02187: beta_tubulin 
Click on image for an interactive view with Cn3D
The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins and a sixth family (zeta-tubulin) which is present only in kinetoplastid protozoa. The alpha- and beta-tubulins are the major components of microtubules, while gamma-tubulin plays a major role in the nucleation of microtubule assembly. The delta- and epsilon-tubulins are widespread but unlike the alpha, beta, and gamma-tubulins they are not ubiquitous among eukaryotes. The alpha/beta-tubulin heterodimer is the structural subunit of microtubules. The alpha- and beta-tubulins share 40% amino-acid sequence identity, exist in several isotype forms, and undergo a variety of posttranslational modifications. The structures of alpha- and beta-tubulin are basically identical: each monomer is formed by a core of two beta-sheets surrounded by alpha-helices. The monomer structure is very compact, but can be divided into three regions based on function: the amino-terminal nucleotide-binding region, an intermediate taxol-binding region and the carboxy-terminal region which probably constitutes the binding surface for motor proteins.
BioAssay Targets and Results
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CID: 6167
AID: 270966
GI: 20455526
IC50: 0.5µM
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Inhibition of [3H]colchicine binding to beta tubulin
Target Name: Tubulin beta-3 chain
Chemical Name: colchicine
IC50: 0.5µM
CID: 44560005
AID: 270819
GI: 20455526
IC50: 0.0005µM
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Inhibition of tubulin polymerization
Target Name: Tubulin beta-3 chain
Chemical Name: Dolastatin-10
IC50: 0.0005µM
CID: 16090892
AID: 273280
GI: 20455526
IC50: 0.64µM
more
Inhibition of tubulin polymerization
Target Name: Tubulin beta-3 chain
Chemical Name: CHEMBL426514
IC50: 0.64µM
Statistics
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PSSM-Id: 100016
View PSSM: cd02187
Aligned: 43 rows
Threshold Bit Score: 683.235
Threshold Setting Gi: 169782427
Created: 31-Jul-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
Hierarchy
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Display:
 
Conserved site includes 30 residues -Click on image for an interactive view with Cn3D
Feature 1:nucleotide binding site [chemical binding site]
Evidence:
  • Comment:In beta-tubulin, E-site (exchangeable) exposed on surface of dimer
  • Structure:1JFF_B: binds GDP (defined using 4.5 A contacts)
    View structure with Cn3D

cd02187 is part of a hierarchy of related CD models.
Use the graphical representation to navigate this hierarchy.
cd00286:Tubulin_FtsZ_Cetz-likecd02186:alpha_tubulincd02187:beta_tubulincd02188:gamma_tubulincd02189:delta_zeta_tubulin-likecd02190:epsilon_tubulincd02191:FtsZ_CetZ-likecd02201:FtsZ_type1cd02202:CetZ_tubulin-likecd06059:Tubulincd06060:misato118372729169782427122379010887943525091369674839005170201757230326184782747200032716914663013794401736959191662104081729843622965571354938928541117460851701894267084417854135499135491135478370382052670811355001354821351203549054135469127906261117459629337144250913551JFF B1354631Z2B B135489244334651947607549053118372729169782427122379010887943525091369674839005170201757230326184782747200032716914663013794401736959191662104081729843622965571354938928541117460851701894267084417854135499135491135478370382052670811355001354821351203549054135469127906261117459629337144250913551JFF B1354631Z2B B135489244334651947607549053
cd02187 Sequence Cluster
cd02187 Sequence Cluster
Sub-family Hierarchy
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 cd02187 Branch
 Whole Hierarchy
 [Download CDTree]
CD Hierarchy
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                ###  ##                                                          ###  # 
1JFF_B         2 REIVHIQAGQCGNQIGAKFWEVISDEHGIDPtGSYHGdsdlq-----leriNVYYne---aaGNKYVPRAILVDLEPGTM 73
gi 72000327    2 KEIINVQVGQCGNQIGAKFWEYISEEHGLQTdGTYKGdngsq-----leriTSYYke---meGRKYVPRAILVDLDPESI 73
gi 51702017    3 REIVNIQAGQAGNQVGEAFWRMLLAEHGLDDaGMYKGndpqq-----iaraGVYFtqvdssgPTKYVPRSVQVDLESGVC 77
gi 18478274    2 REIIQLQIGQCGNQIGTKFWEEIAQEHRIDEnGQLIEqahef-----qqylDVYFne---vnGNRYIPRNVLIDLEPGTL 73
gi 57230326    3 REIINVSVGQAGNQIGTAFWENILQEHGLDRsGKATTddphi-----ldkvDVYFte---asNKKYVPRSIQVDLEPGVV 74
gi 108879435   2 REIVTLHLGQCGNKIAQTFWETICEEHCLNErGQFIGkhflp-----lqriNVYFee---apCCNFVPRAIFADLEPGAM 73
gi 67483900    2 REIICLQIGQCGNQVGEKFWDIIGQEHGLSPdGIFNGnklq------qqrlNVYFse---ssTKRYVPRSINVDLEPGTL 72
gi 1223790     3 REIVHVQVGQCGNQIGNVFWETMCKEHHLAEdGKFVPsqnkhhdqirldkiGVYFre---agEKRYVPRAILVDLEPGIL 79
gi 25091369    2 REIVTLQIGGAGNAIGDSFWHVISHEHGVDYaSGRFGgtspl----qleriNVFFna---taSKRFYARTILIDTEASTI 74
gi 169146630   2 REIVFLQVGQSGNNCGAKFWELIADEHGVDPnGRYVGdsdlq-----leriNVYFne---atGGRFVPRCVLVDLDPHTM 73
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                               ###                                      #######         
1JFF_B        74 DSVRsgpfgqIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLIS 153
gi 72000327   74 NYVRstqygkLFDPENAVSGESGAGNNWSRGYYEQGAEIVDKVLSVIRREAEAADSLEGFQLIHSLGGGTGSGLGSLLIS 153
gi 51702017   78 NRLRsgplgqLFRPDTYFTSDSGAGNNWAKGYYTEGAELIDGILDIVRRQCEATEALQGFQMIHSLGGGTGAGLGSLLLS 157
gi 18478274   74 DSVRasrygkLFRPDNFVFSQGGAGNNFAVGHYTNGTEILGEVLDVIRHQVESSDCLQGFQLTHSLGGGTGSGFGTLLLS 153
gi 57230326   75 DLVRsgplanLFRPDTFVHGESGAGNNWAKGYYTEGAELVDPVLDVLRQQAENSDSLQGFQLLHSLGGGTGSGLGALLLG 154
gi 108879435  74 VGLKcsrfgqLFSPESMVNGMLGAGNNWARGYHTEGAEMLDRIMNVARKMVEGCDCFQGFQMVHSIGGGTGSGLGTLMME 153
gi 67483900   73 DTIKsgkmghFFKPDSFIFGHGGAGNNWAKGHYTEGTEICETIMESVRKEAEACECLQGFQVTHSLGGGTGSGLGTLLLS 152
gi 1223790    80 EVIKaaptgkMFKPDNFIFGASGAGNNWGKGHYTEGAQLIEECVEVVRREAESCDAPQGFQITQSLGGGTGSGLGTLLLN 159
gi 25091369   75 QRLNas--sqLYRPENFVAGSESAGNNFARGYHTDGAAILDQVLENTRREVESVDSLQGFQLLHSIGGGTGSGLTSLIME 152
gi 169146630  74 ESVRgtpmgqLFRPDNYIIGQAGTGNNWARGRYTDGAEQADSVMDVVRKEVESCDLIQGFQVIHALGGGTGSGMTCLLLE 153
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                       # ##       ##                      #   #              #  ##  #   
1JFF_B       154 KIREEYPDRIMNTFSVVPSPKvsdTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLk-lTTPTYGDLNHLVSAT 232
gi 72000327  154 KLREEYSDKTLSTCSIIPSAKvsdTVVEPYNAILSMPHLMDNCDENFCIDNEAIFDICQYNLkleNRVTYGDLNHLASLA 233
gi 51702017  158 KLREEYPDRMLSTFSILPAPNvseTVVEPYNSLLSIHQLVDNCDLTICIDNEALYDIAVRTLk-iKSPGYKDLNQLIAKV 236
gi 18478274  154 KLQEDYCDRIFQSYSVFPSIKvsdAVVEYYNTAVSVNHLIENCSQCMSIDNEALYNICTNTLk-lKNTCYGDLNHIISAS 232
gi 57230326  155 KIREEYPDRMLATFSIFPSPKvseTVVEPYNAVLSTHNLVENSDITCCIDNEALYNICVSDLk-iQSPEYKDLNSLIAKV 233
gi 108879435 154 NMKDEFPRKILNTFSVIPSEKvseVVVEPYNAVFALNSMTDCSDETFCLDNEALYNINMTTLr-vDKPTIDDLNHLISMA 232
gi 67483900  153 KLKDEFFEKVISTFSVVPSPTvseTVVEPYNCTLSVHKLLESSGVTFCFDNEALFKIASDIMk-ePKPSYESLNSLISSV 231
gi 1223790   160 RIRDGFPDRITATYSVYPSPKvsdVVVEPYNATLSIHQIIENGDETFVIDNEALYNISHNVLk-qAQPKYKELNWVISLV 238
gi 25091369  153 ALVEQYPDNLLCNYVTIPSPNmsqVVVEPYNALLSTPALVNNSHLTFCLDNEALFQICNRNLk-lKMSGYEHINHIVALT 231
gi 169146630 154 QIKESWPDRIVNTFSIFPSPKvsdVVVEPYNAIFTIDLLLSLSDETLVLDNEALHSICTNVLn--ISASFNDINHLMSTC 231
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1JFF_B       233 MSGVTTCLRFPGQLNADLRKLavNMVPFPRLHFFMPGFAPLTsrgsqqyralTVPELTQQMFDAKNMMAaCDPRHGRYLT 312
gi 72000327  234 LSGITTFQRFKGNLKTDIRKL--NTAGSPRLHFFMTSFAPVYgkgiidcqafSISDLTQQVLDAKNIMT-CNHNQGKFLS 310
gi 51702017  237 MCGVSTSLRFPGQLNGDLRKLgmNLVPFPRLHFLMPSFAPFYdpkartfqrlSVSELTSSLFDKKNLLVaSDPRFGRYLT 316
gi 18478274  233 MSGTTCSLRFPGQLNSSLRKLsvNLVPFPRLHFFTTSFAPLTsrhsmqyrslKISEVSDQMFDPQNQMCaINCKQGRYLT 312
gi 57230326  234 MTGFTTTLRFPGVLNSDLRKLavNMVPFPRLHFFTAGYAPLVanasksytasNVHELTAAIFQKRSLLAaIDPLFGKYLT 313
gi 108879435 233 MSGITCSFRYPGQLNSDLRKLltNMVPYRKLHFFVPGIAPLTskesqcyrslSVSELVYQIFDEQNLMAaCSPSRGKYLT 312
gi 67483900  232 MSGITCSLRFPGQLNQDLKKLtvNMIPYPRLHFFSSSIAPVSsalsmqyeslSVQEIIQQLFDRKNMMVdLDPASGKYLT 311
gi 1223790   239 MAGITSSLRFPGKLNGDLRKMavNLVPFPRLHFFLIAQSPLFapgegakvklTIQEITDQMWSSRNFMAnVVPGDGKYLS 318
gi 25091369  232 MSGITTCLRFPGQLNAGLRKIyvNMVPFPRLHFLIPGFAPLVtckqqqfskgTVSELVQQIFYSNNLLCaIDLRKGKLLT 311
gi 169146630 232 VGGMTTCFRFPGQLNSDLRKLavNMVPFPRLHFFAPCFVPLSsrgnqpykaiTVPNLVQQMFDAKHLMAyCDPREGRFLT 311
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1JFF_B       313 VAAVFRG-RMSMKEVDEQMLNVQNKNssyfveWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRR 391
gi 72000327  311 SAIIYRG-QQTEKKDAEQIISVENEDpsemieSLPKSTNTDVCDIPSRGLKTSATFIANSTAIQEPLKRISKQFAGLFRR 389
gi 51702017  317 AACIFRG-KVSSHEAENSVMQLQRKNsnlfveWIPDNVSVSLCSVPPVGQPQAAVALANSTCMQELFKRNLDQFALMFKR 395
gi 18478274  313 GACLFRG-QFSSSEVEKQITQYQKKNpqnqvqWLPNNMKVSQCNVSQRGVVNSGTFVGNTTAINEMFRRIYNNFQAMFKR 391
gi 57230326  314 VSVAYRG-KLSMRDIENAVWDFHNKNsehfvpWIPNSSLTTLCTVPPLGQTAAATLVANTTAISEVFKRSHTQFRSLFRR 392
gi 108879435 313 AAALFRG-RVSTRNVEEQIANVRQKNhgtfshWIPNNVKSAICDIPPAGMKMSATFIANTTAITQLFQRLLNQFGTMYRR 391
gi 67483900  312 ASCIMRG-KVSTHDVEEQLYKIREKNpdafvpWIPNNMQLAVCDIPPKGLNLSGTFIGNSTSIMDLFQRVNSQFLSMFKR 390
gi 1223790   319 ASLAYRGaDIPSQEVDGIVKSIQEKMaedfvnWIPNNIKSSIISVPPEDTLLSGTFVANTTAIKGVFQRISSAFARMYKR 398
gi 25091369  312 AAGIFRG-RMSPREVDQLMTGVRNKNinnfvdWIPNNIKTAICDIPPRGLKMSATFIGNTTAIQTLFQRLLDASMSMLRR 390
gi 169146630 312 CASVFRG-RLSMAEVEESMLNVQDKNsryfveWIPNNVQNAVCDIPMRGQKMCATFLANTTALQTVFRRIQQAFSAMFKR 390
                        410       420       430
                 ....*....|....*....|....*....|....*
Feature 1                                           
1JFF_B       392 KAFLHWYTgegMDEMEFTEAESNMNDLVSEYQQYQ 426
gi 72000327  390 KAFLHWYT---MEESEFTDAENKVNDLISEFQQYE 421
gi 51702017  396 RAFLHWYTgegMDVMEFTEAESNTQDLISEYQQYQ 430
gi 18478274  392 KAFLHWYTnegMDELEFTEAGANVQDLISEYLQYS 426
gi 57230326  393 RAFTHWYTgegMDEQEFTEAEANLSDLCIEYDQYA 427
gi 108879435 392 KAFLHWYTgegMEEKEFVDAEQSLRELIKEYESQE 426
gi 67483900  391 KAFLHLYTeegMEESEFSEATADLIDLVNEYRQYD 425
gi 1223790   399 KAFLHWYKgegMDEMEFEESDKNVRDLVTEYQDKQ 433
gi 25091369  391 KAHLHWYTgegMEEQEFQDAQQELQAIIDDYRSSA 425
gi 169146630 391 KAFMHWYSgegMEESEFVSTESNVNDLISEYQEYQ 425

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