3NUQ,3ONN,3OPX


Conserved Protein Domain Family
HAD_5NT

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cd02604: HAD_5NT 
Click on image for an interactive view with Cn3D
haloacid dehalogenase (HAD)-like 5'-nucleotidases similar to Saccharomyces cerevisiae Phm8p and Sdt1p
This family includes Saccharomyces cerevisiae Phm8p (phosphate metabolism protein 8) and Sdt1p (Suppressor of disruption of TFIIS). Phm8p participates in the ribose salvage pathway, it catalyzes the dephosphorylation of nucleotide monophosphates to nucleosides, its preferred substrates are nucleotide monophosphates AMP, GMP, CMP, and UMP. Phm8p is also a lysophosphatidic acid phosphatase, dephosphorylating lysophosphatidic acids (LPAs) to monoacylglycerol in response to phosphate starvation. Sdt1p is a pyrimidine and pyridine-specific 5'-nucleotidase; it is an NMN/NaMN 5'-nucleotidases involved in the production of nicotinamide riboside and nicotinic acid riboside, and is a pyrimidine 5'-nucleotidase with high specificity for UMP and CMP. This family belongs to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319791
View PSSM: cd02604
Aligned: 17 rows
Threshold Bit Score: 231.753
Threshold Setting Gi: 731454
Created: 23-Jun-2004
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:

Feature 1           #####              #                             #    #                     
3NUQ_A       59 VFFFDIDNCLYKSS.[2].IHDLMQQSILRFFQT.[2].KLSPEDAHVLNNSYYKEYGL.[1].IRGLVMFHK.[18].I 140
3ONN_A       32 VFFFDIDNCLYKSS.[2].IHDLMQQSILRFFQT.[2].KLSPEDAHVLNNSYYKEYGL.[1].IRGLVMFHK.[18].I 113
3OPX_A       32 VFFFAIDNCLYKSS.[2].IHDLMQQSILRFFQT.[2].KLSPEDAHVLNNSYYKEYGL.[1].IRGLVMFHK.[18].I 113
gi 3322425   11 HILFDIDNTLYSCT.[2].IEMAITQRIHTFVAH.[2].HVSCEEARALRQRTKHLYAT.[1].FEWLKAEHN.[18].E 92
gi 1723966   57 VFFFDIDNCLYKSS.[2].IHDLMQQSILRFFQT.[2].KLSPEDAHVLNNSYYKEYGL.[1].IRGLVMFHK.[18].I 138
gi 1351649    7 IIFFDLDNCLYPKS.[2].IHNMMAARITAFFSD.[2].GIPTEEAERLREVYYRHYGI.[1].IRGLVLHHE.[18].V 88
gi 30697234  17 CLLFDIDDTLYPLS.[2].LAMEVKKNIQEYMVQ.[2].GIEEDKVQELCLSLYKIYGT.[2].AGLKAVGYD.[17].T 98
gi 79456826  10 CLFFDMDDTLYPLS.[2].INLACRNNIQEFMLN.[2].GIEESEVPKLCLDLYKEYGT.[2].AGLKVMGYE.[17].K 91
gi 83594672  14 TWLFDLDNTLYPAS.[2].LFAQIDLRMKAYIGR.[2].GLPPEEAFRLQKHYYHTYGT.[1].LRGLMDEHA.[16].V 93
gi 56707911   3 TYIFDLDNTLYSYK.[2].LFDSQMARMSEYIKL.[3].ISDIEKANAIRDELYYEFGS.[1].MLGMMRYHN.[16].H 83

Feature 1                                 ###                                          #        
3NUQ_A      141 LKPDIPLRNMLLRL.[5].IDKLWLFTNA.[1].KNHAIRCLR.[1].LGIADLFDGLTYCDYSR.[ 6].KPHVKAFEK 212
3ONN_A      114 LKPDIPLRNMLLRL.[5].IDKLWLFTNA.[1].KNHAIRCLR.[1].LGIADLFDGLTYCDYSR.[ 6].KPHVKAFEK 185
3OPX_A      114 LKPDIPLRNMLLRL.[5].IDKLWLFTNA.[1].KNHAIRCLR.[1].LGIADLFDGLTYCDYSR.[ 6].KPHVKAFEK 185
gi 3322425   93 LQYDPMLRPFLQSL     HMPLTALTNA.[1].RVHAQRVLD.[1].FHLSDLFLDVFDITYHA.[ 3].KPHHSCFVR 156
gi 1723966  139 LKPDIPLRNMLLRL.[5].IDKLWLFTNA.[1].KNHAIRCLR.[1].LGIADLFDGLTYCDYSR.[ 6].KPHVKAFEK 210
gi 1351649   89 IKKDEVLREMLLEL.[2].KYKCWIFTNA.[1].IVHANRVLK.[1].LGIEDCFDGITYCDYNA.[ 5].KPMPEMYER 156
gi 30697234  99 LKPDPILRNIILSL     PIRKVVFTNA.[1].KAHAAKIIA.[1].LGLEGCFEKIISFETLN.[39].KPSEGAFEQ 198
gi 79456826  92 LKPDPLLRNLLLSM     PHRKIIFTNA.[1].KAHATRALN.[1].LGLEDCFEGIICFETLN.[11].KPSVEAFEA 163
gi 83594672  94 LAADPVLGGLLARL     PGRKIVFTNG.[1].TRHALAVLD.[1].LGITDHFEAIHDIAASG.[ 3].KPQPACYDD 157
gi 56707911  84 FEPNQKLNKYINDL.[2].NNRTYIFTNA.[1].DFHASRVLK.[1].LGLDNSFDGVLTIEDTG.[ 3].KPKTKYFEI 149

Feature 1                          ##  ##                
3NUQ_A      213 AMKESGLARY.[2].AYFIDDSGKNIETGIKLGMKTCIHLV 250
3ONN_A      186 AMKESGLARY.[2].AYFIDDSGKNIETGIKLGMKTCIHLV 223
3OPX_A      186 AMKESGLARY.[2].AYFIDDSGKNIETGIKLGMKTCIHLV 223
gi 3322425  157 TLEAVHKTVQ.[1].TLFVDDCLMHVRAFIALGGHAVLVDE 193
gi 1723966  211 AMKESGLARY.[2].AYFIDDSGKNIETGIKLGMKTCIHLV 248
gi 1351649  157 VMREAGVTDK.[2].CIFVDDSYGNILGAREFGWKYTVQLV 194
gi 30697234 199 VFKMANINPK.[1].TLFFDDSIRNIQTGKRVGLHTVWVGT 235
gi 79456826 164 AIRIADIVDP.[2].TIFFDDSIRNIASAKATGLKTVFVGE 201
gi 83594672 158 LIARYGLDPA.[1].TIMVEDSHKNLQPAAALGMTTLLVRN 194
gi 56707911 150 GRDKFDIDFT.[1].AIFFEDSSHNLVPAKHLGMQTVLVHA 186

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