Helix located at the middle N-terminal region of Sfi1p
Sfi1p is a single continuous alpha helix wrapped with centrins, which are calmodulin-like proteins present in centrosomes and spindle pole bodies (SPBs). Sfi1p is localized to the half-bridge of the yeast SPBs, and like Cdc31p (yeast centrin), it is required for the early steps of SPB duplication. Sfi1p contains a central domain composed of multiple conserved repeats which all can bind to Cdc31p. The Sfi1p C-terminus has an important role in separation or splitting of the duplicated SPBs during bipolar spindle assembly.
Feature 1:centrin binding site [polypeptide binding site]
Evidence:
Structure:2DOQ; Saccharomyces cerevisiae Sfi1p-centrin complex; contacts at 4A.
Comment:The Saccharomyces cerevisiae centrin, Cdc31p, binds Sfi1p on multiple conserved repeats; both proteins localize to the yeast spindle pole bodies (SPBs) half-bridge, where the new SPB is assembled.
Jiyao W et al.(2023). "The conserved domain database in 2023.",