1FIU,4ABT


Conserved Protein Domain Family
NgoMIV-like

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cd22340: NgoMIV-like 
Click on image for an interactive view with Cn3D
Restriction endonuclease NgoMIV and similar endonucleases
Type II restriction endonuclease NgoMIV recognizes and cleaves the palindromic sequence 5'-G|CCGGC-3' (| denotes the cleavage site) to produce 4 bp 5' staggered ends. It is active as a homotetramer and belongs to a superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.
Statistics
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PSSM-Id: 411744
Aligned: 48 rows
Threshold Bit Score: 235.975
Created: 28-Mar-2011
Updated: 30-Sep-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1FIU: Neisseria gonorrhoeae restriction endonuclease NgoMIV active site binds substrate DNA and divalent cations; contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                             #          
1FIU_A        28 GVVSNADGSNTRSFNIakgiadll---------------------------hsetvseRLPGQTSGNAFEAICSEFVQSA 80  Neisseria gonor...
WP_052069387  15 GLPNTSDASDKLSIEIgsalfehlgvpvdqpatpvakewavamaddlqerlsipaphlLIQAERKLLHFEQYAHLDVARS 94  Streptacidiphil...
SEC10503      15 GRPNTSDKNDKGSVELgkaffev----------------------------mgvspnaEPPEQPVGNLMAAAVAADLQQG 66  Streptomyces sp...
ELB87749      31 GNPSTSDSHDPGSIEWghalfdalgvipgtpevsgv---gdamekraaahlaslrpdlMLGQSRPALEFDQYRHLNVFRQ 107 Rhodococcus wra...
WP_072736853  17 ALPSTSDKGDVNSIRWgramfda-----------------------------lgvahaTPEISDVGTALEVAVVEDLRAR 67  Rhodococcus tri...
WP_038675754  33 GKPNTSDKTKSDSVALgfalfda-----------------------------lgvrrdTPGQDETGLPFAQRVAADLAAR 83  Pimelobacter si...
WP_110943817  15 GKPNTSDSSKPESVELgnclfekigveegkesvkidhiwshsmrddlkekltataphlVVDRERAADDFEQYAHLNALKQ 94  Streptomyces niger
EUA15588      34 AFPNTSDAHDGLSVELggalfeqlgispdkpaptgs---gsdmevrlvnhlrdlrpdlHIERSRAAREFSQYAHLDVFPN 110 Mycobacterium k...
ROP77402      17 GNPNTSDNNDDQSKYLgkslfeavgvpdgqevgddp---gknleaavaadlaprrpdlDLELSRAVTDFDQYRHLGVFGN 93  Frigoribacteriu...
WP_120494883  16 GAPNTSDNNDPQSKELglelfrqmmvpdgkpgasdp---gsaleaavladlssrrpdlLIERSRSVANFAQYRHLAVFPA 92  Microbacterium ...
Feature 1                                                                                        
1FIU_A        81 FEKLq------------------------------------------hIRPGDWNVKQVGSRnrleia----------ry 108 Neisseria gonor...
WP_052069387  95 LANDdglevagala------------------------aldaltaadsLLGGEPLVQAVNSVraa--------------- 135 Streptacidiphil...
SEC10503      67 LQFEaphlsvvperpft-------------------sfeqfshlnatrELRGDMATQVLHAAaalrkqtqkldvaeaere 127 Streptomyces sp...
ELB87749     108 FSKTyrgpaae-------------------------------ldqalaELPPTPEFHSVRAH------------------ 138 Rhodococcus wra...
WP_072736853  68 RPDLivdrsriatdfdqyrhlsqlknymssfhddldriemaiaetkqlDTSKSVTALRRQLNti---------------- 131 Rhodococcus tri...
WP_038675754  84 LSASghgllvapegvvntfqq-----------yrhvgaladiavepsaAYEKAWRRLTAEARkrviap-----------p 141 Pimelobacter si...
WP_110943817  95 LTADdsleisaal---------------------------erlqshlsLEHGQWDAEGAQLVqe---------------- 131 Streptomyces niger
EUA15588     111 FRRNfg-----------------------------------------dLRPALDDLTTRVLAvel--------------- 134 Mycobacterium k...
ROP77402      94 FRKGyapsssdi----------------------------eefirladNLPTSPATTRLRNVlqqsr------------- 132 Frigoribacteriu...
WP_120494883  93 FRKTykpvseam----------------------------aalgaiasALPKSREASKLQAAlrrts------------- 131 Microbacterium ...
Feature 1                                                  #                                     
1FIU_A       109 qqyaHLTALAkaa-----------eenPELAAALGSDYTITPDIIVTRNliadaeinrneflvdeniatyaslragngnm 177 Neisseria gonor...
WP_052069387 136 --vaGADERR-----------------KLLIDLMADESPLNLDLAVERPtiaavv-------------------pgtvll 177 Streptacidiphil...
SEC10503     128 qieiHLSRINdelha-------aegrrRELLDLLGEESLLKLDITVSREmpnala--------------------peapl 180 Streptomyces sp...
ELB87749     139 ----ILAAIAaagqd--------hqlvTQLQDTMPEESLLRIDITVAAPep----------------------------v 178 Rhodococcus wra...
WP_072736853 132 --rnHAASNRgf--------------fCALKENLAEESMLRTDIAVTSPrs----------------------------g 167 Rhodococcus tri...
WP_038675754 142 aavtRLEQLIedverat---saevearQTALAEIGTESLLKLDVAVAQDqrp--------------------------rl 192 Pimelobacter si...
WP_110943817 132 ---lSQAVERsna------------rrRPLLDLLGDESLPRLDIAVHRPsmpea----------------------hsdi 174 Streptomyces niger
EUA15588     135 --gpHRDRIErdlhdlasstdtqaalvDDLAEQMPEESLLKIDISIAIPheg--------------------------el 186 Mycobacterium k...
ROP77402     133 srfeGRDYLV-----------------SQMLDNMPEESLLRVDVTVGQHrse-------------------------arp 170 Frigoribacteriu...
WP_120494883 132 trfaAQDALT-----------------SRMVGEMPEEAILKVDLTVGQPrga--------------------------gd 168 Microbacterium ...
Feature 1                 #             #                                                        
1FIU_A       178 PLLHASISCKWTIRSDRAQNARSEGLNLVRNRKGRLPHIVVVTAEPTPSRISSIALGtGEIDCVYHFALYELEQILqsl- 256 Neisseria gonor...
WP_052069387 178 PHLVAGFSLKWSLRTDRLQDPRTQGSQMAAMRRGRMPHFAAVTMEPRPYYLGRLAMGtGDLDCVYHLALPELSKAVeevy 257 Streptacidiphil...
SEC10503     181 PHLVAGLSLKWSLRTDRAQDCRSQGAKMTALRRGRMPHFAAVTVEPRPAMLALLGRGsGDIDCVYHLNLPALGTAIdnyc 260 Streptomyces sp...
ELB87749     179 DRLLIGLSSKWSLRTDRAQDCVSQGSKLVNLRRGHMPHYAVLTMEPRPSMLKLIAYGsGAVDCIYHLALPELREAAmrie 258 Rhodococcus wra...
WP_072736853 168 QRLLVALSSKWSLRTDRAQDCVSQGSKLVSLRRGHMPHFAVITMEPRPSMLRLLTDGsGSVDCVYHVAFGALKTAAnsls 247 Rhodococcus tri...
WP_038675754 193 PELEIGLSLNWSLRTDRAQDCRSQGAKLSALRRGRMPHFAAVTMEPRPYMLNLLGGGsGDVDCVYHLHLPALQAAVddvy 272 Pimelobacter si...
WP_110943817 175 PHLVAGLSLKWSLRTDRLQDPRTHGAKMAALRRGRMPHFAAVTMEPRPYFLARLGQGtGDLDCVYHLHLPALVSAVddfy 254 Streptomyces niger
EUA15588     187 DELAIAISSKWSLRTDRAQDCVSQGNKLVAQRRGMMPHFGVLTIEPRPAMLKILADGsGAVDYVYHLDLPALEATIeava 266 Mycobacterium k...
ROP77402     171 PLMHLGLSAKWSLRTDRAQDCISQGSKLATQRRGAMPHFAVITMEPRPSMLKILADGsGAIDCVYHLHLPALIEAVevqr 250 Frigoribacteriu...
WP_120494883 169 PHMEIGVSAKWSLRTDRAQDCISQGARLASQRRGAMPHFAVLTMEPRPAMLRILADGsGAIDCVYHFDLPALTRAIdvva 248 Microbacterium ...
Feature 1                                               
1FIU_A       257 ---------nyEDALDLFYIMVNGKRLKDISDLPLDLAV 286 Neisseria gonorrhoeae
WP_052069387 258 v------gpkrAKQLDTFHRLVDQRRLRDYDELVTYLAT 290 Streptacidiphilus albus
SEC10503     261 sg---strktrLEIRDKFNRLRDQRRLRDYDELRSYLAT 296 Streptomyces sp. 2224.1
ELB87749     259 stkktkagaagWPQKLMLERMITQGRIRSYQQLVEEVLR 297 Rhodococcus wratislaviensis IFP 2016
WP_072736853 248 kq----siprmPEQLDLLDRMIKQNRIRPFSALLDEINL 282 Rhodococcus triatomae
WP_038675754 273 gr---ktdrtsQRIVAVFNRLVEQRRVRDYDELVAHAER 308 Pimelobacter simplex
WP_110943817 255 t-----gnrsrMKLRDVFHRMVDQRRIRDYDDLVEYLGT 288 Streptomyces niger
EUA15588     267 e-----nkprgWSPRQTFRRLMTQRRLRDFDHLVYEVMR 300 Mycobacterium kansasii 732
ROP77402     251 kkr-tsahgrpWSPGVTFDRLIRQRRLRDYDELVTAMDA 288 Frigoribacterium sp. PhB107
WP_120494883 249 s-----rkkgsWSPKRTFDRLVLQGRIRDYSELVESVER 282 Microbacterium phyllosphaerae

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