2OA9,2OAA


Conserved Protein Domain Family
MvaI-like
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cd22337: MvaI-like 
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Restriction Endonuclease MvaI and similar endonucleases
Restriction endonuclease MvaI recognizes the sequence CC|WGG (W stands for A or T, '|' designates the cleavage site) and generates products with single nucleotide 5'-overhangs; it belongs to a superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.
Links
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Statistics
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PSSM-Id: 411741
Aligned: 13 rows
Threshold Bit Score: 243.395
Created: 7-Jan-2019
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
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active siteDNA binding
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:2OAA: Kocuria varians restriction endonuclease MvaI active site contacts substrate DNA and divalent cations; contacts at 4A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                     #    ###                           
2OA9_A         8 LNLLKEAIQNVVDGGWHETKRKGNTGIGKTFEDLLEKEEDNLDAPDFHDIEIKTHEtaAKSLLTLFTKSPTn----PRGA 83  Kocuria varians
2OAA_A         8 LNLLKEAIQNVVDGGWHETKRKGNTGIGKTFEDLLEKEEDNLDAPDFHDIEIKTHEtaAKSLLTLFTKSPTn----PRGA 83  Kocuria varians
WP_113844503   6 INDLVEVVNKIKEDGWHDATRKGNTGIGKTFEDLLEKEEDNLDAPDYFDIEIKTHEtaSMSMITLFTKSPSy----PRGA 81  Enterococcus fa...
WP_125833946   7 TQHLLEVFSEIKEAGWHESRRKGNTGIGKTFEDLLEKEEDNLDEPDFYDIEIKTHDenAASLVTLFTKSPTn----PRGA 82  Streptococcus
AUJ33388      15 INSQPTEFDFGDGKVWYKSHRNGPTGIGKTFEDLLGKKEDNLPLPDFHQIELKAHDqaENSYITLFTKSPNv----PRGV 90  Lactobacillus n...
WP_029597583   6 LLELKKKFIEIKNMGWVTATRSGNTAIGKTFEDLLGKMEDNLAEPDFGDIEIKSQRelSKSFVTLFTKAPSy----PKSA 81  Fusobacterium n...
WP_125426275   7 LKELEDKFHQIKSKDWNLSSRSGPTGVGKTFEDLLDKKEDNCAKADFGEYEIKTHL--GKTRITLFTKAPImd-gkKRGV 83  Streptococcus
CCX35725       8 IEELNKLFLSINKMGFVESKFKGSGAAGKTFESLIGKRGDSKCLPDFKGIEIKTHInnVKYPITLFSCMPDtnevnSNYM 87  Clostridium sp....
CDB91667      12 IKKLNNEFNRIKRMGYVPEVHRGYGGIGDTFERLLGKEKSDFCVPDYDGIEIKTRRsfSKGLIGLFGSVPDg---eELFE 88  Clostridium sp....
CDF20097      34 MKIFCQQFYYIQALNWVKSSGNGSAAAGKTFEDLVGKAQDRMFLPDYLGIEIKTKSvsAKRKLCLFNMAPDs----EPNI 109 Clostridium sp....
WP_057197238  15 INTAISEHNFGDGRVWYKSHRNGPTGIGKTFEDLLGKQEDNLPLPDFHDIELKAHDndMDSNITLFTKSPNi----PKSA 90  Lactobacillus k...
WP_150280159   7 VEEFKMEFERISGLGFIKSTRKGPTGIGKTFEDMLDKIEDNEDAPDFGEIEIKAHDtiGNSMVTLFTKAPTn----PKQS 82  Leuconostoc car...
WP_107382249   5 INDLIFAIKEIQKKDWHNSTRNGSTAIGKTFEDLLEKEEDNNDLPDFYDIEIKTHEtvINSMLTMFTKSPTy----PKGA 80  Staphylococcus ...

Feature 1                                                                                        
2OA9_A        84 NTMLRNRYGKkd--eyGNNILHQTvs-gnRKTNSNSYn----yDFKIDIDwesqvVRLEVfd---kqDIMIDn------- 146 Kocuria varians
2OAA_A        84 NTMLRNRYGKkd--eyGNNILHQTvs-gnRKTNSNSYn----yDFKIDIDwesqvVRLEVfd---kqDIMIDn------- 146 Kocuria varians
WP_113844503  82 NTLLRTKYGKld--ifNNKILHVTv----SGNKITQSkey-sfNFKIVVDdtnrlIRLHVyd---kdKNIIDd------- 144 Enterococcus fa...
WP_125833946  83 NTYLRENFGMln--efGLRTLHQTv---sATQRTNSRvy--dfDFMIQVDrl--nQRVYIcvy-nklGELIDn------- 145 Streptococcus
AUJ33388      91 NTLLRERYGYkdi-nsDQKILHTSv---tSKMQFNAKs----nRYFMIVNdp-erESLILrvy-thsRELIKdd------ 154 Lactobacillus n...
WP_029597583  82 NTYLREKYGDkn--eeNLRTLHTSif-rdENIYKERY------SFSLEIDen--eERIYIkvkdlntKNTEQ-------- 142 Fusobacterium n...
WP_125426275  84 NLEIRDKYGIasktfpEVKTFFTSl----SSKKLTATdgkyshQFKLEVNrg--eKRLYLlv---ydNGKLIs------- 147 Streptococcus
CCX35725      88 LINFVKNCGHyg---iNNKNIYFN-----SKIYVNNIclslnhFTKSYIDfk--kQEIIIdfv-sydLELIN-------- 148 Clostridium sp....
CDB91667      89 LDRLREKYGYpdkeirSAKVLYSVvygnmLCQVGTKY------FFKLDVDrk--eEKVFLcvf-dknLNLLEr------- 152 Clostridium sp....
CDF20097     110 IRTLWSKCGWrsk-knDLREFFATv----DALNFSNEhr--kyAFKLKVDydnkcIRLCIrkn-wddVDEIDd------- 174 Clostridium sp....
WP_057197238  91 NDLLRDLYGYkn--dtNVKELHVSv---kSTKQFNSKsn---hYFQICDNp---dKKAVT-------LEVFDdkgnrcsn 152 Lactobacillus k...
WP_150280159  83 NTYLRERYGVmdt-efGVKTLHMTv----SAIQKTKSeky-dyDFQIRVNrk--eKRIIQmvf--dkTGTMId------- 145 Leuconostoc car...
WP_107382249  81 NTMLRENYGInd--ehDNKILHVTvs-gkQKTNLNKYn----yNYLIEVNk---eKEIIKlniydkkDNLVNd------- 143 Staphylococcus ...

Feature 1                         #                                                              
2OA9_A       147 --SVYWSFDSLQNQLDKKLKYIAVISAESKien--------ekkYYKYNSANLFTDLtvqslc---rgiengDIKVDIRI 213 Kocuria varians
2OAA_A       147 --SVYWSFDSLQNQLDKKLKYIAVISAESKien--------ekkYYKYNSANLFTDLtvqslc---rgiengDIKVDIRI 213 Kocuria varians
WP_113844503 145 --SVYWTYNDLQTQINKKLETIAIISAESRihd--------gkkQYKYNEIDIVTGLsvdclv---eaikagDLKVDIRI 211 Enterococcus fa...
WP_125833946 146 --HIFWSFEDIQRQLERKLKKIALVSGNRKeen--------dkvYYKYNRIHLITGFnidnli---laiengDMKIDIRI 212 Streptococcus
AUJ33388     155 -fIAEWSYDILKKSLNNKLKTLAVINSSTKvy-----------nKIKYYSYDNIKIIyalsleqllaaledqKLIVDLRL 222 Lactobacillus n...
WP_029597583 143 --LVYYSFETIKNKLDKKLKKLAYVEAKTKylnn-------gkeAFYYTKMTMYENPslenfl---nlikenKIMIDIRI 210 Fusobacterium n...
WP_125426275 148 -kEYYWEFSDFTTLLEKKLKKIAIFSADSRkiittidgksnseiFYRYKKMQIFTGLnldkfl---kgleegSIIVEPRM 223 Streptococcus
CCX35725     149 --KMAWPIKKIEKIIESKIKTLAIITVNRIqne--------estKFKYKSIEYYEYKgnksfi---eslknrKIYISFNL 215 Clostridium sp....
CDB91667     153 --SVYWSFFTLKNKLYCKLRYLMLVDAWTNnid--------gkmHYKYYKATFYKLKdidifv---dlidkgFIAVTFKI 219 Clostridium sp....
CDF20097     175 --RFFWTFQQLESRIHVKLSYLALIHYESKmsygesyfkftsldMYRYRYFEKFLELie-----------agKITVDFKI 241 Clostridium sp....
WP_057197238 153 diSIEWSYDSLENSLNNKLKTLAIIQASSQkyn--------gdvYYSYESIKIIHGFnlksll---salkddKLIIDLRL 221 Lactobacillus k...
WP_150280159 146 -dSVYWTFESLEKQLIKKLGTLGLVDVEVQkkl--------dgtYYHYVGVQYIKGLtidnmv---kaiedgAMKIDNRI 213 Leuconostoc car...
WP_107382249 144 --SVFWSFNDLEKQINKKLKTIAIISAESRikd--------nqkQYKYNEVDIVTNLsiesli---ngiengDIKIDIRI 210 Staphylococcus ...

Feature 1                                         
2OA9_A       214 GayhsgkkkgKTHDHGTAFRINMEKLLEYGEVK 246 Kocuria varians
2OAA_A       214 GayhsgkkkgKTHDHGTAFRINMEKLLEYGEVK 246 Kocuria varians
WP_113844503 212 GayqsgikkgKTHDHGTGFRINMKRLLNYATVK 244 Enterococcus faecium
WP_125833946 213 GvyktgrnygKTHDHGTGFRILDSDLIKYANVI 245 Streptococcus
AUJ33388     223 GvyhsgknlgKTHDHGTGFRIKYNDLLQYAYTE 255 Lactobacillus nagelii
WP_029597583 211 GvyhdlkkygKTHDHGTGFRIKEENFCELYKCK 243 Fusobacterium nucleatum
WP_125426275 224 GyynsgtkkgQVHDYGTAFRIHFTDLLKYGECK 256 Streptococcus
CCX35725     216 Nlqri-nnknKLKYHGISFVINIENISSLYRKI 247 Clostridium sp. CAG:1000
CDB91667     220 SvyrgnyrygLPHNHGLTFGISNEHLPLLYDEY 252 Clostridium sp. CAG:302
CDF20097     242 LthgdgddfgKPNDKGTSFIISEDDLDILFEKI 274 Clostridium sp. CAG:609
WP_057197238 222 GayksgknagKKHDHGTGFRLKYKYLLEYSQIQ 254 Lactobacillus kunkeei
WP_150280159 214 GayktgskkgKTHDHGTGFRMTQNALLKYGTVF 246 Leuconostoc carnosum
WP_107382249 211 GayktgknkgKTHDHGTGFRIALKDLIKYANVE 243 Staphylococcus chromogenes

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