1D02


Conserved Protein Domain Family
MunI-like
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cd22336: MunI-like 
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restriction endonuclease MunI and similar proteins
MunI ( E.C. 3.1.21.4) is a type II restriction enzyme that catalyzes the hydrolysis of DNA, recognizing the palindromic hexanucleotide sequence CAATTG (with the cleavage site after C-1), and is very similar to EcoRI. They belong to a superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.
Links
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Statistics
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PSSM-Id: 411740
Aligned: 15 rows
Threshold Bit Score: 306.956
Created: 28-Mar-2011
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1: active site [active site], 6 residue positions
Conserved feature residue pattern:D E [IV] K [RS] RClick to see conserved feature residue pattern help
Evidence:
  • Structure:1D02: Mycoplasma MunI active site interacts with CAA trinucleotide and cuts between C and A; contacts at 4A
    - View structure with Cn3D
  • Comment:conserved residues in these columns may be essential for endonuclease activity
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                        
1D02_A         2 GKSELSGRLNWQALAGLKasGAEQNLYNVFNAVFEGt---KYVLYEKPKHLKNLYAqvvlpddvik------eifnplid 72  Mycoplasma
WP_007686624   2 GTEALRGRAVWQDYSGANagFAEQSFFAVFTEAFRGt---ELSIRAKPQEFKHIYVdvpladdvi--------aeiynpe 70  Sphingobium ind...
WP_083236029   2 GSEANRLRKKWQHYSGEQagRAEQSFFDAFSNAFTDt---EYTIRAKPKEFSRAYVdvqlpsqvl--------seiynps 70  Halomonas sinai...
CDE86384       2 GSFANRFRRKWQDYGGSKatVAEHSFIEVFSELFKDt---EYEVVSKPTDFKNLYVdvelseeel--------aaiytpd 70  Prevotella sp. ...
WP_104722441   2 GKEELKNRENWQNQSGMQaiNAENRFFNVFNQYFFYnl-kHFQIIAKPRELDNIYKniilpkeie--------akiynpp 72  Helicobacter me...
WP_075347222   2 ASNELRGRANWQTISGGKalKTEEIFAKALQIALDGaypgVFKVDQHPKEFGDIYSkhqlpqqtlda---iynvsitksn 78  Streptococcus s...
WP_081032414   2 ATQELRKRANWQTISGAKalGTEKLFQKSLQSALDSvypgQFVVDRHPKEFGDIYStydlpqevldkiynvdvtakkang 81  Flavonifractor ...
WP_102748146   2 ATQELRKRANWQTISGAKalGTEKLFQKSLQTALDSvypgQFVVDRHPKEFGDIYStydlpeelcnkiyninvtakkang 81  Akkermansia muc...
WP_065236852   2 ATQELRKRANWQTNSGLKalKTEELFQHVLQSSLDIvypeKFLVDRHPKEFSDIYStyplpeeelkkiynidvtekkrng 81  Gallibacterium ...
ABK77229       2 GKKELRDRDVWQKKSGLMgkKAEDEFEAVFLAEFTGt---EYVIRKEPKELKDIYS------------------------ 54  Cenarchaeum sym...

Feature 1                  #              ####                    #                              
1D02_A        73 lSTTQWGVSPAFAIENTETHKILFGEIKRQDGWVE-GKDPSAGRGNAHERSCKLFTPGLLKAYRTIGGINdEEILPFWVV 151 Mycoplasma
WP_007686624  71 cGITKHGITPDYAIDNKGAGKTLYVEVKRQDGWVE-GKPRSAGRGNAHERSNKFFTPGLLKTLRKRGNLG-PDALPFWTV 148 Sphingobium ind...
WP_083236029  71 ePIKKHGVFPDYAIDNKETGKTIYIEVKRQDGWIE-GGQRSDGRGNAHERSCKFFTPGLMSLLRKEGNIS-EPNLPFWTV 148 Halomonas sinai...
CDE86384      71 ePITKHGIQPDGAIRNTRTGKTIYVEIKRQDGWVE-GKSRSAGRGNAHERLCKYFTPGLLNKLRTTGKIS-APNLPFWIV 148 Prevotella sp. ...
WP_104722441  73 nGYGKHGISPDYAIKNIITNKTLYVEVKRQDGWVE-NKEMSAGRGNAHERACKLFTPGLLNILRENGNIK-NIPLPFWII 150 Helicobacter me...
WP_075347222  79 gKDYQWGISMDFAIRNLQSGKTLFGEIKRQDGWIE-GGNMQDGRGNAHERSNKYFTPGLLKVIREKSGIS-DEILPFWVV 156 Streptococcus s...
WP_081032414  82 kPKYQWGISMDFAIRNITNGKILFGEIKRQDGWIE-TTNMQAGRGNAHERSCKYFTPGLMKVIRETGGLS-DEILPFWLV 159 Flavonifractor ...
WP_102748146  82 kPKYKWGISMDFAIRNITNGKVLFGEIKRQDGWVE-TTNMQAGRGNAHERSCKYFTPGLMKVIRETGGLS-DEILPFWLV 159 Akkermansia muc...
WP_065236852  82 sAKYQWGISMDFAIRNLENGKILFGEIKRQDGWVE-TTNMAAGRGNAHERSCKYFTPGLMRIIRKTGGLS-DEILPFWLV 159 Gallibacterium ...
ABK77229      55 -KNPKHGVSIDYAITNKKTKKTLYVEIKSQEGYVPgETLPKDGRGNAHERSCKFFTPGLLKVMCKLSNLP-VGTLPFWIV 132 Cenarchaeum sym...

Feature 1                                                           
1D02_A       152 FEGDITRDPKRVREITFWYDHYQDNYFMWRpNESGEKLVQHFNEKLKKYLD 202 Mycoplasma
WP_007686624 149 FQGDIARDPCRVREITLWYDGYPGHYFMWRdNSDPTALLRHFDEHLRPLLA 199 Sphingobium indicum
WP_083236029 149 FLGNITRDPCRVREITCWYYGHEGNFFFWRdPSNPEPLFEHFEAHIAPILR 199 Halomonas sinaiensis
CDE86384     149 FQGDITRDPCRVREITYWFNDFKSNYFFWRnTTKAEDLIDHFETYIAPLLE 199 Prevotella sp. CAG:891
WP_104722441 151 FQGDIARDPKRTREIFCWFSGYENHFSLWSnQDSDELVINHFKQYFKYFLD 201 Helicobacter mesocricetorum
WP_075347222 157 FVGDITRDPRRNREIDFWYQGFERNYFMWRdTTDIDSLLQFFEENLLDYLL 207 Streptococcus sp. 'caviae'
WP_081032414 160 IVGDITRDPRRNREIAFWFQNYTKNYYMWRdTDDVGDMLDFFENNLLPYLL 210 Flavonifractor plautii
WP_102748146 160 IVGDITRDPRRNREIAFWFQNYTKNYYMWRdTDDVGDMLDFFENNLLSYLL 210 Akkermansia muciniphila
WP_065236852 160 IVGDITRDPRRNREIAYWFQEYTKNYYMWRnTNDIGELLDFFENNLLPYLL 210 Gallibacterium genomosp. 3
ABK77229     133 YQGRITRDAKRTREITYWFDEYGAHFFLWK-SSSSTPLINHFKKNLRHILD 182 Cenarchaeum symbiosum A

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