1YFI,1SA3


Conserved Protein Domain Family
MspI-like
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cd22334: MspI-like 
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Restriction endonuclease MspI and similar endonucleases
The type II restriction endonuclease MspI It recognizes and cleaves the palindromic tetranucleotide sequence 5'-C|CGG (| denotes the cleavage site) leaving 2 base 5' overhangs. It belongs to a superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.
Links
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Statistics
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PSSM-Id: 411738
Aligned: 4 rows
Threshold Bit Score: 372.108
Created: 15-Jan-2019
Updated: 30-Sep-2020
Structure
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Program:
Drawing:
Aligned Rows:
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active siteDNA binding
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1SA3: Moraxella sp. restriction endonuclease MspI active site binds substrate DNA and a cation; contacts at 4A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                       #                                             
1YFI_A      1 MRTELLSKLYDDFGIDQLPHTQHGVTSDRLGKLYEKYILDIFKDIESLKKYNTNA-FPQEKDISSKLLKALNLDLDNIID 79  Moraxella sp.
1SA3_A      1 MRTELLSKLYDDFGIDQLPHTQHGVTSDRLGKLYEKYILDIFKDIESLKKYNTNA-FPQEKDISSKLLKALNLDLDNIID 79  Moraxella sp.
P11405      1 MRTELLSKLYDDFGIDQLPHTQHGVTSDRLGKLYEKYILDIFKDIESLKKYNTNA-FPQEKDISSKLLKALNLDLDNIID 79  Moraxella sp.
TCM68663    1 MRTEILTNLYDEFEIDSLPLEQHGKTSDRLGKLYERYTLEIFKDFKTMIFYSDPIlYPQEAKIVKDILEALNIYLEDISD 80  Acinetobacter calc...

Feature 1                      # #                 # #                                        
1YFI_A     80 VSSSDTDLGRTIAGGSPKTDATIRFTFHNQSSRLVPLNIKHSSKKKVSIAEYDVETICTGVGISDGELKELIRKHQNDQS 159 Moraxella sp.
1SA3_A     80 VSSSDTDLGRTIAGGSPKTDATIRFTFHNQSSRLVPLNIKHSSKKKVSIAEYDVETICTGVGISDGELKELIRKHQNDQS 159 Moraxella sp.
P11405     80 VSSSDTDLGRTIAGGSPKTDATIRFTFHNQSSRLVPLNIKHSSKKKVSIAEYDVETICTGVGISDGELKELIRKHQNDQS 159 Moraxella sp.
TCM68663   81 VSSSNDGLGRTIAGGLPKTDAYITIHLYDGTKKQVPLNIKHSSKSKVSIAEYDVETICEGVGIPDGELKELIRKHQNDQS 160 Acinetobacter calc...

Feature 1                                                   #                                 
1YFI_A    160 AKLFTPVQKQRLTELLEPYRERFIRWCVTLRAEKSEGNILHPDLLIRFQVIDREYVDVTIKNIDDYVSDRIAEGSKARKP 239 Moraxella sp.
1SA3_A    160 AKLFTPVQKQRLTELLEPYRERFIRWCVTLRAEKSEGNILHPDLLIRFQVIDREYVDVTIKNIDDYVSDRIAEGSKARKP 239 Moraxella sp.
P11405    160 AKLFTPVQKQRLTELLEPYRERFIRWCVTLRAEKSEGNILHPDLLIRFQVIDREYVDVTIKNIDDYVSDRIAEGSKARKP 239 Moraxella sp.
TCM68663  161 AKFFTSAERHRLIELLDPYKERFVRWCITLNAEKSEENLLHPDLIIRFKVVDREYKGVSIRNIDDYVNDRIKEGARKRRP 240 Acinetobacter calc...

Feature 1                            
1YFI_A    240 GFGTGLNWTYASGSKAKKMQFKG 262 Moraxella sp.
1SA3_A    240 GFGTGLNWTYASGSKAKKMQFKG 262 Moraxella sp.
P11405    240 GFGTGLNWTYASGSKAKKMQFKG 262 Moraxella sp.
TCM68663  241 GFGTGLNWTYASGSKSKKMQFKG 263 Acinetobacter calcoaceticus

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