4RVE


Conserved Protein Domain Family
EcoRV-like
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cd22323: EcoRV-like 
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Restriction endonuclease EcoRV and similar endonucleases
Type II restriction endonuclease EcoRV recognizes the site 5'-GAT|ATC-3' (| denotes the cleavage site) and functions as a homodimer; it belongs to a superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.
Links
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Statistics
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PSSM-Id: 411727
Aligned: 61 rows
Threshold Bit Score: 224.738
Created: 28-Mar-2011
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:4RVE: Escherichia coli restriction endonuclease EcoRV active site contacts substrate DNA; contacts at 4A
    - View structure with Cn3D
  • Citation:PMID 8491171
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                     
4RVE_A      3 RSDLINALYde-----------nqKYDVCGIISAEgKIYPLgSDTKVLSTIFELFSRPIINKIAEKHg-----yIVEEPK 66  Escherichia coli
GBC91746   15 ADAFLKRLQkqveqlncllsgqgrDWAIRGVIDTFqQIYALsADTKLISKIMEIVLLPHMLQFAQKHk-----yKTVLSP 89  bacterium HR15
OYT75241   12 QLRELAPKIeslln------spsgEWSARGVIDLQqTIYPLsYDTKLISKVIELMILPILIGFADQYg-----yRTEIAP 80  Armatimonadetes ba...
RLC66919    9 EFRALFTTLeqelq------tedgLWTLRGFVDTAqRVYSLtTDTKVISKALELMLLPRISGFWEQRg-----yEVVPAK 77  Chloroflexi bacterium
RJQ26636    4 KLDLKKILEeeia-------liglGWDIKGVIDSQnRIYTInSDTKLISKVFELVTTPIILDIGEKYk-----iKVIQSD 71  Candidatus Parcuba...
OQY86398   10 LLDYFDELKdrvv-------tkdgDWTVKGFIDIYrRIYTIsVDSKVLSKVLELLMFPVLQQFAQRNg-----fRVILAQ 77  Anaerolineae bacte...
PIV64793    4 FNFAQSLKAnleanl----ltedkDWNIKGFIDVHkNIYPIsLDTKLLSKILELTTIPIVTSFAKKYr-----lKVYLAQ 74  bacterium (Candida...
PIN85934   10 KQVIISELKs-------------fRWDILGIIDEKqNIFPMtSDTKVLSKVFEMKITPDFRRIASKLsvkgnpcTLVLAK 76  Candidatus Woesear...
KYC54888    4 KEEIKRELDelf---------tdfEWDIKGLIDKNnNIKPLpKDSKVFTLIFENKGKDIIKTFADAHn-----lSLEESS 69  Arc I group archae...
PKL53823   36 KANLKKDLEqlf---------sdfKWDFIGIADDKqNIIPIpKNSTCITAIIEQKSLEILDKFVKSKyk----cELIRAP 102 Candidatus Methano...

Feature 1           #               # #                                                       
4RVE_A     67 QQnHYPDFTLYKpsepnKKIAIDIKTTYTnke------neKIKFTLGGYTSFi--RNNTKNIVYPFd---qYIAHWIIGY 135 Escherichia coli
GBC91746   90 KQnYYPDITFIDdtphrHKFALDIKSAYRlsd------teVSMMTLGAFTGYfrnRRSRKNITLPYe---eYSAHFVLGI 160 bacterium HR15
OYT75241   81 EQnYYPDITFIDqa--kCKFALDIKTTYRiesk----egeVSAMTLGTFTGYfrnRESRKNILYPYk---eYSGHFVLGI 151 Armatimonadetes ba...
RLC66919   78 YQnFYPDLSLVRa---eERYALDLKTTYRllrrgggvpsrVSGFTLGAFTGYfrhRDSTKNVTFPYg---sYRQHYVVLI 151 Chloroflexi bacterium
RJQ26636   72 RQtVYPDLTLVLpt--gEKIALDIKSTYRktk------drVAGFTLGSYTGYl--RNPTKNIMFPYh---eYSEHLVLGF 138 Candidatus Parcuba...
OQY86398   78 AQnQYPDLTLVStsnenLCFAVDIKTTYRtkvdq-dglmrVNGMTLGTFGGYfrnRDRPVNSVMAYa---rYAKHYVLGV 153 Anaerolineae bacte...
PIV64793   75 HQlQYPDITLEGaeiknKKYAADIKTTYRvgg------klKGGFTLGSFRGSlrsPLSTRYSRFPYs---qYAKHWILGI 145 bacterium (Candida...
PIN85934   77 EQnQYPDITLSGgilgdEKIAIDIKSAYRkge------gkFSGFTLGAFNGYfknRNSTKNIAFPYk---eYARHWVLCF 147 Candidatus Woesear...
KYC54888   70 TR-EYPDVTLIEnifngKMLAIDFKSAQKkdn-----gtsTTKMTLGSFMGYf--RHPERKLSGCKyaygkYSQHWIIGF 141 Arc I group archae...
PKL53823  103 TTrEYPDAILRGgifedKIIALDIKTARKvgt------drISGLTIGSYAGYf--LHPDEKRAGCSipygnFDEHWIVSF 174 Candidatus Methano...

Feature 1                                                        #                            
4RVE_A    136 VYTRvatrksslktyNINELneIPKPYKgVKVFLQDKWvIAGDLAGSGNTTNIGSIHAhyKDFVEGKGi-----fDSEDE 210 Escherichia coli
GBC91746  161 IYTRndssinrsrayALKELnsIPAVISdILLFVQYKYkITSDKPGSGNTKNIGAITRi-EDLVKGRGpf---aeLGEEV 236 bacterium HR15
OYT75241  152 IYTRadihqenlkpiPIDALetVRAVIRdIRVFVEHKYkIASDRPGSGNTKNIGSVRQi-ELLLKGEGpf---aeHGEEV 227 Armatimonadetes ba...
RLC66919  152 VYTQlrgqt--pgiyPLERLsdIMPPIRdIEIFIHEKWrVANDRPGSGNTRNIGSITDl-AALREGIGpfvrlgeEGEVI 228 Chloroflexi bacterium
RJQ26636  139 IYTReedkd--geivPLTKIdeIKPVIKdIEIIVQEKYkLSADRPGSGNTANIGSSSDi-EILRNGTGvf---akHGEKV 212 Candidatus Parcuba...
OQY86398  154 VYSRaenid-erqvyEIEHLtdIPSVASdFQFFLHEKYrIASDGPGSGNTKNIGSTKYl-DRLLNGTGvf---akLGEAV 228 Anaerolineae bacte...
PIV64793  146 IYTRkkeie-qkriySLDDLpnINSVITdLEIILQEKYrIANYVPGSGNTANIGSVANi-EMLRNGTGpf---akYGDKV 220 bacterium (Candida...
PIN85934  148 IYERdretd-vtamfSLKDAkkIPKIITdVEIILQDKWkVAKTTPGSGNTANIGGIDSl-EKLKNGTGpf---ssEGEDV 222 Candidatus Woesear...
KYC54888  142 IYKWd----------TSQDT--LNIVSD-VEVIINEKWkVASRTTGSGNTAHIGSVTDi-SKLKEGRGe-----fNSEVE 202 Arc I group archae...
PKL53823  175 LYKW-----------DSRKN--SKEMVSeIDCVVNLKWkMASKSTGTGTTKHIGSVKSi-QALKDGIGg-----fDSEAE 235 Candidatus Methano...

Feature 1              
4RVE_A    211 FLDYWRNYE 219 Escherichia coli
GBC91746  237 FDDYWMHYL 245 bacterium HR15
OYT75241  228 FDEYWMNYQ 236 Armatimonadetes bacterium JP3_11
RLC66919  229 FNEYWQQYM 237 Chloroflexi bacterium
RJQ26636  213 FEDYWRNFL 221 Candidatus Parcubacteria bacterium
OQY86398  229 FNDYWMNYR 237 Anaerolineae bacterium UTCFX5
PIV64793  221 FQDYWVNYL 229 bacterium (Candidatus Ratteibacteria) CG01_land_8_20_14_3_00_40_19
PIN85934  223 FNDYWMHYI 231 Candidatus Woesearchaeota archaeon CG10_big_fil_rev_8_21_14_0_10_44_13
KYC54888  203 FEQYWRQFA 211 Arc I group archaeon ADurb1013_Bin02101
PKL53823  236 FLKFWRNKG 244 Candidatus Methanoperedenaceae archaeon HGW-Methanoperedenaceae-1

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