1NA6,3HQG


Conserved Protein Domain Family
EcoRII-like

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cd22322: EcoRII-like 
Click on image for an interactive view with Cn3D
Restriction endonuclease EcoRII and similar endonucleases
Restriction endonuclease EcoRII recognizes the sequence 5'-CCWGG-3' (W stands for A or T); it requires binding of a second target site as an allosteric effector in order to be active. EcoRII belongs to a superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.
Statistics
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PSSM-Id: 411726
Aligned: 22 rows
Threshold Bit Score: 219.878
Created: 14-Jan-2019
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:3HQG: Escherichia coli restriction endonuclease EcoRII active site contacts substrate DNA; contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
1NA6_A       188 DWHLRFPSGSEIIQYAAshyvkn--------------sldpdEQLLDRRRVEYDIFLLVEELHVLDIIRKGfg------- 246 Escherichia coli
3HQG_A         6 DWHLRFPSGSEIIQYAAshyvkn--------------sldpdEQLLDRRRVEYDIFLLVEELHVLDIIRKGfg------- 64  Escherichia coli
WP_117817549 188 DSFPKGIDFTRYSISTLgeldw---------------inepdKCLLDCYEREEVLFKVFERHLLERDLAPYlgnd----- 247 unclassified Co...
WP_117612035  68 EIQEKIYDHTEYLRINPd------------------------LKLIEYTRVEYALFRHMEQAKYMDSVRDGfd------- 116 Bifidobacterium...
KRF38938      59 EHLMAKIAGKRVSHKTGfvavesfctavrsllpevdgrdadeAIATWMQVTEELYFLYEESVIQRRLDDAFanrt----- 133 Terrabacter sp....
WP_014961880 194 EWFKKFPSGAEIIKKTLefrplh--------------geipdKRLLKRRECEYEIFQSVEEAIELPVIRRGfs------- 252 Leptospirillum ...
WP_088826046  14 NDQLAKEVADEIFAGKApeeik----------------shfsDYVTQIHDRIYEKYVLAERRAGQQVIDEQierhs---- 73  Marinobacter sp...
WP_032514797  26 DIFKKMVPSSKEFVDKQflllkeelt-------pkelklnfsHYVQLLHDQIYADFLKIQKTGFNKAFSDDlesg----- 93  Prochlorococcus...
WP_035022443  20 VPKAAELTGDALNALLGakandae-----------yikdnfsQLIHDTQIKAYHLYLEHEARESGAAIRDVvmpllpsga 88  Aquamicrobium d...
OFI35424      10 DDFKSYLPTNEQLCRSSaervigehk-------kdyvmsnfsELLEEMQEEIYKSYLKEQEVSGQKVIDEQidkfg---- 78  Alteromonas lip...
Feature 1                                           #                                #           
1NA6_A       247 -----------svDEFIALANSVSNRRKSRAGKSLELHLEHLFIeh---glRHFATQAITeg--nKKPDFLFPsagayhd 310 Escherichia coli
3HQG_A        65 -----------svDEFIALANSVSNRRKSRAGKSLELHLEHLFIeh---glRHFATQAITeg--nKKPDFLFPsagayhd 128 Escherichia coli
WP_117817549 248 ----------ldvDGVLRTTMSAFQRRKSRAGTAFEHQLEYLFKgw----gISYSAQPYTeg--kSRPDFIMPsidaykk 311 unclassified Co...
WP_117612035 117 -----------dlDAFVQLALSLLNSRKSRAGKGFENQIGAILTan----gLLFQEQVKTeg--sKRPDFVFPsgaayhd 179 Bifidobacterium...
KRF38938     134 ---------kidiKTFTSVASSVLNTRKSRAGGAFESHLSAVFRan----gIAFTRVTKPlrsdkTKPDFLMPslslyqd 200 Terrabacter sp....
WP_014961880 253 -----------svSEFVDRAQTILQRRKSRSGKSLELHLKEIFLedglsegRDFSCQAESep--gKRPDFIFPseaayrd 319 Leptospirillum ...
WP_088826046  74 ---------tidfSEMSKLMMSLSQSRRSRAGQAFEGIFKALFYkl----sYPYSDQVNIe---gAKPDFVMPseeffr- 136 Marinobacter sp...
WP_032514797  94 ---------elsfEKFDKFFLSLSQSRKVRSGGVFELIIENLLKfl----eYPIDTQKKId---nKRVDFVLPsvdfyk- 156 Prochlorococcus...
WP_035022443  89 taddvfsflenrfHAIDRLCLSLSQSRRTRAGSTFETIVTTLFRkl----gYPHTPQPQIn---gSRPDYVLPslahyl- 160 Aquamicrobium d...
OFI35424      79 ---------kinfSDVDKFLMSIFQSRKSRAGKAFEFIIRELFIra----gVPFSEQITVd---gAKPDFVMPsaeyfe- 141 Alteromonas lip...
Feature 1                     #            #                                                     
1NA6_A       311 tefpvenlRMLAVKTTCKDRWRQILNEadk--ihqVHLFTLQEGVSLAQYREMRESGVRLVVPSSLhkkype-avraelM 387 Escherichia coli
3HQG_A       129 tefpvenlRMLAVKTTCKDRWRQILNEadk--ihqVHLFTLQEGVSLAQYREMRESGVRLVVPSSLhkkype-avraelM 205 Escherichia coli
WP_117817549 312 plypveklTMLGAKTTVKERWRQVLEEanr--ienKHLITLDVAVSTEYTDSMREHNLQLVVPRPVfgsytt-eqqnwlM 388 unclassified Co...
WP_117612035 180 mgrspdklVVLAAKTTCKDRWRQILTEakrmenepKWLITLQQGNSVQQLNEMMEDNVRLVVPEPYirsypa--eyrgsI 257 Bifidobacterium...
KRF38938     201 ddfqtgllSTVAAKRTLKERWLQVASEgkr--iptKHLATVDRDVASDTILGMPSHNIVLVMPRSLrdtryw-lnardvW 277 Terrabacter sp....
WP_014961880 320 psfpseklRMLAAKTTCKDRWRQILNEadr--iqvKHLLTLQDGVSENQFREMSESGVQLVVPKSLhdsypp-nirqnlL 396 Leptospirillum ...
WP_088826046 137 --tnpldsIIFTAKRTLRERWRQVVTEank--gygFFLGTFDDKISANQISQAARHKIYIVVPASMkkenpnyreaynvL 212 Marinobacter sp...
WP_032514797 157 --knplntVLLSAKRTTRERWKQVVPEvsq--snqFYLATIDKKITSDQLKEMEKYRVFVINTLQNidsishykeaanmV 232 Prochlorococcus...
WP_035022443 161 --ayssdcIIFTCKRTLRERWRQAITEgvt---gqFFLATVDDQLSVQQIDAMKEKNVVLVVPAGLkntayk--drmnvV 233 Aquamicrobium d...
OFI35424     142 --erpldcMLFTAKRTLRERWRQVVTEank--sysYFLATLDEKVTESQLKQAATHKLYIVVPAQIkesnpvysssynvL 217 Alteromonas lip...
Feature 1                  
1NA6_A       388 TLGAFIAELT 397 Escherichia coli
3HQG_A       206 TLGAFIAELT 215 Escherichia coli
WP_117817549 389 DVGEFCQLLV 398 unclassified Collinsella
WP_117612035 258 WPFKKFIGYV 267 Bifidobacterium pseudocatenulatum
KRF38938     278 TLEDLLSMLL 287 Terrabacter sp. Soil810
WP_014961880 397 TLKDFIYELR 406 Leptospirillum ferriphilum
WP_088826046 213 TFEDFLKLHL 222 Marinobacter sp. es.048
WP_032514797 233 SFEHFLDFTL 242 Prochlorococcus marinus
WP_035022443 234 SFETFFDHYL 243 Aquamicrobium defluvii
OFI35424     218 SFEQFFEIHL 227 Alteromonas lipolytica

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