Restriction endonuclease EcoO109I and related endonucleases
EcoO109I is a type II restriction endonuclease that recognizes ds DNA with a seven-base pair motif of both degenerate and discontinuous sequence, RG|GNCCY (R = A or G, Y = T or C, with | designating the cleavage site), and generates 5'-overhangs; it belongs to a superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.
Structure:1WTE: Escherichia coli restriction endonuclease EcoO109I active site contacts metal ion and substrate DNA; contacts at 4A - View structure with Cn3D
Jiyao W et al.(2023). "The conserved domain database in 2023.",