2FQZ


Conserved Protein Domain Family
Ecl18kI-like
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cd22320: Ecl18kI-like 
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Restriction endonuclease Ecl18kI and similar endonucleases
Restriction endonuclease Ecl18kI recognizes the sequence |CCNGG and cleaves it before the outer C (| designates the cleavage site) to generate 5 nt 5'-overhangs. It belongs to a superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.
Links
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Statistics
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PSSM-Id: 411724
Aligned: 24 rows
Threshold Bit Score: 299.55
Created: 10-Jan-2019
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:2FQZ: Enterobacter cloacae restriction endonuclease Ecl18kI active site contacts substrate DNA; contacts at 4A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                        
2FQZ_A         9 FKTLISKERKSHfi---tPFALVYKTFCDLGydqknsdYFLNNPSEYIIAMRKNCWKEFEPFEKEFTTRMLSYLIDEERI 85  Enterobacter cl...
PIR80006      16 WKEKIEEHRIRLvn---kPSYYVEKIIKERKin---keDLEKNIEGILLSLREEAWQTYLIDEALFNSEIISHLFVNRNL 89  Candidatus Levy...
PWL73822      15 LSSEIKNYRRKYi----nTPKNYLKILEKNNctd--fsYLKSNISKVIEELREGCWQLYLKDEAEFNKNFISHLIETRKL 88  Candidatus Gast...
RLD47436      19 FEKLIKQYRKQYir---nSKDIVKQVLDEHKyfi--eeIENKSISKIILGIRELAWNEYLKDEVEFNVVLMKNLHLVLET 93  Bacteroidetes b...
WP_086239219  15 FSKFIKSTRNKIip---kPKDILIKTLLKHDlk----tIKLDTISQTITQLREYSYDEFLTYENEFNSVILKNIASKFNI 87  Campylobacter s...
KFL86304       7 FKKSIKSNRNALvp---kPRDLVQEALRNSPyydkdqsYFLDNATEILYGVRAGCWNTYLKHEKEFNIKMGQILVKQDSD 83  Bacillus cereus
WP_056830780   7 KSKVIPKLRKRHie---tSAEMINEAMQVYGfdikpqsYFLNNSSDFIMTMRDILWNNFRNAEKKFNAAATTALFDNPQL 83  Paenibacillus s...
WP_101731518   3 RKTFILPPKETVk-----KVLSEHSDLMEAL-------NVERDVSIVVRTLREEAFRSFLTQEAAMNAYLLNHISEKFAV 70  Carnobacterium ...
WP_078387730  18 YYGFLKTVRKGIiktpkdNIDIVTNKFNNDIaq----lLKNKEFTKAIFLIREETYKEFLKQEHEFNTYVLKSLAIKFDI 93  Campylobacter p...
SEH74676      73 FEKLIKSCRSKYir---tSQEIVDKVFKEKPvyl--qnIKNKEVSQSILGLRGLVWNEYLKDEVSFNIILMQKMHATLKT 147 Bathymodiolus a...

Feature 1                                                                        #               
2FQZ_A        86 k-------------------------dMSPYDAIRDFTMEYPTHIYDLALSNTQSRRSRAGKEFESILELLMMGAGIPVD 140 Enterobacter cl...
PIR80006      90 pktilsellnreffnldleklskeeieIKIAKIVGDSTGRVFPYIYQLSLSTTNSRRTRSGKVFETLIESFFDILQYPYE 169 Candidatus Levy...
PWL73822      89 pcsllqdyikt------ydicnsknftTDLSELIGKYTGSIMPYIYALNLSTTQSRRARSGKTFEAIIKYLIVEKFKYPF 162 Candidatus Gast...
RLD47436      94 pkriieaivdn-----kvinysgeeliREVRDICGEYAGRVYPYIYKISLSNTNSRRSRSGSTFEAIIYTVYEALGYPYD 168 Bacteroidetes b...
WP_086239219  88 adeiltliakdf---fknlpsdydsfkASFSLYFGRYAGEISPYIYELCLSNTQSRRSRAGKIFEAIIYYLYEYFNYSFN 164 Campylobacter s...
KFL86304      84 iqn---------------------sqnNNAEEIIGSFLEKYSDHLFELSKSNTNSRRARAGKEFEAIIEAILLRSGIFFD 142 Bacillus cereus
WP_056830780  84 meqlntll-----------ydvslddkQKLVNIVTGFSSNYEEHLFELNKSNTNSRRSRAGKEFEGLLKKILIKSNISFD 152 Paenibacillus s...
WP_101731518  71 pkkkfdqlyd-------ifsqsdneikSRLRVFFGDYAGQIYPYIYRLSLSNTQSRRSRAGKVFEGIIYGLYDVLNYPYE 143 Carnobacterium ...
WP_078387730  94 pkikmdaisdel---ysiskkgkydfkTELINKFGDYASLISPYIYILSLSNTQSRRSRAGKTFEYTIYYIYEYLGYPYN 170 Campylobacter p...
SEH74676     148 pqhilellvnk-----kitelkekdllKEVRSLCGEYAGRVFPYIYKIALSNTNSRRSRAGKTFESIVYKVYDILEYDFD 222 Bathymodiolus a...

Feature 1                            #                     #            #                        
2FQZ_A       141 VQGAIgksff-qknqIGKLVDLVMPGVVQYTSNKRNTMLISAKTTLRERWQEVPEEVNRTGIREMYLATLDDSFSEETIN 219 Enterobacter cl...
PIR80006     170 TQSSIssge---ywsLGKKVDLIVPDVEKYSKERAKCAVVTMKTSLRERWQEVAEELHRTSVPHIYLLTLDPKVSGNLID 246 Candidatus Levy...
PWL73822     163 DDQSNigqgffktqnVGKIVDGIIPNKNAYINNRAKTLIITMKTSLRERWQEVAEEIGRTNTPHMFLLTLDKKLSKQKLK 242 Candidatus Gast...
RLD47436     169 SQSKVgrkif-telgLGKKVDSILPNVGCFEKRRNKTIIGTMKTSLRERWQEVAEEIERTKIPEIHLLTVDENISYNKAS 247 Bacteroidetes b...
WP_086239219 165 SQAKVgkraf-sdlgLGKVVDSILPSVEAFKNFRNKTIVGSMKTTLRERWQEVVEEIDRSNLPNIYLLTCDTDISTSKIT 243 Campylobacter s...
KFL86304     143 NQGVIgsklf-eterLGKLVDCVVPGVIEYSLEKRKCSLISMKTTLRERWQEVPEEMKRTGAQEMFLLTLDDGITKNTIE 221 Bacillus cereus
WP_056830780 153 DQGAVgtkkf-seggLSKIVDYVVPGVLEYGIEKTHCALISMKTSLRERWQEVPEELSRTGAHFMYLFTLDRDISNEVIT 231 Paenibacillus s...
WP_101731518 144 SQATIgsknf-qskkLGKIVDSLLPNLEAFEQRRDKVVIGTMKTTLRERWQEVIEEINRTGLPSIYLLTVDEDISSSKVD 222 Carnobacterium ...
WP_078387730 171 AQSQIgknaf-snmgLGKIVDSILPSADAFSEFRNQCIIGSMKTTLRERWQEVVEEINRSNLPNIYLLTTDNNISENQIK 249 Campylobacter p...
SEH74676     223 SQNKVgrktf-tklgLGKKVDSILPSVDCYEQRRNKTIIGTMKTSLRERWQEVAEEIERTKIPEIHLLTVDEDISKSKAQ 301 Bathymodiolus a...

Feature 1                                                     
2FQZ_A       220 ILYEANVVVVTTVENKNFkykn--nnrvLTFEDMLQSAMELSRKW 262 Enterobacter cloacae
PIR80006     247 TIKRYNITLVILKKDKERfprs--dnvkDYETFFKKEIPYIANYW 289 Candidatus Levybacteria bacterium CG10_big_fil_rev...
PWL73822     243 TMELHNIRIVNTKDVKEAykdy--nniiSFDEFFNEEIPDCLNYW 285 Candidatus Gastranaerophilales bacterium
RLD47436     248 EMSKHNIIVVAFDWVANSkplkemrniiSFEEYFFEEIPLILKFW 292 Bacteroidetes bacterium
WP_086239219 244 QMSQHNIVLVVLNSVKSKlenf---hnvITFESYFAHEIPQILNY 285 Campylobacter sp. P0136
KFL86304     222 SLASHNITLVVPDDLKLKyyeg--hlsiYGLTQFLYELIERNQLW 264 Bacillus cereus
WP_056830780 232 SLSEHKIYLVVLDVHKNDyyss--dnriYGLSELLTELKRLTEYW 274 Paenibacillus sp. Soil787
WP_101731518 223 QMANHNVILVVYRDVKAQkalvn-krnvVSFEDYFFEEIPQNLKY 266 Carnobacterium maltaromaticum
WP_078387730 250 QMREHNIILVVNKEVKDTflny---rnvISFETYLTTNIPQVLKY 291 Campylobacter pinnipediorum
SEH74676     302 EMANHNIIVVAYDWVANSeslts-mkniISFEDYLFEEIPSILKF 345 Bathymodiolus azoricus thioautotrophic gill symbiont

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