5EAN


Conserved Protein Domain Family
DNA2_N-like
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cd22318: DNA2_N-like 
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Nuclease domain of the nuclease/helicase DNA2 and related nucleases
The eukaryotic nuclease/helicase DNA2 processes double-strand breaks in DNA that have single-stranded ends/overhangs, as well as Okazaki fragments and stalled replication forks; it is therefore crucial for maintaining the integrity of the genome. The nuclease domain modeled here belongs to a superfamily of PDDEXK nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.
Links
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Statistics
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PSSM-Id: 411722
Aligned: 129 rows
Threshold Bit Score: 225.86
Created: 2-Jul-2020
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
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active siteDNA binding
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1: active site [active site], 5 residue positions
Conserved feature residue pattern:[DK] E [FLIMV] K QClick to see conserved feature residue pattern help
Evidence:
  • Structure:5EAN: mouse DNA2 active site binds substrate DNA and divalent cations; contacts at 4A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                         
5EAN_A        130 SGTSVASSIrCLRRAVLSETFrgsdpatrQMLIGTILHEVFQKaise-sfaPERLQELALQTlreVRHLKEMYRLnlsQD 208  house mouse
XP_014340488  523 SGTSIANSIrCMRRAVLSERFkvcdqstkQMLFGTLVHEIFQKaatdygfgEDGLRELASKTisnPKCLREMYSLnltEV 602  coelacanth
XP_007897282  561 SGTSVANSIrCMRRAVLSERFkacergtrQMLFGTLVHEIFQKaatshgftEDALQKITSKTlraLKYLGEMYSLklsQA 640  elephant shark
TSK22614      368 SGTSIANTVrCMRRAVLGEMFksldggsrQMLNGTIVHDIFQKaamsldfsPEKLEKLASEAllrPNYLGQMYSLkltQA 447  goonch
XP_019639379  804 SSTAVANGIkCLRRAVLSERFkgtdgsskQMLLGTILHDVFQAatst-sfsADSLKQISQQAlqkQRHLRDMFSLsltQS 882  Belcher's lan...
XP_030830072  830 TGTAVSNSIrCMRRSVLSEHFkglergnrAMLLGSLLHEVFDQailkkefqKAQLMRIASDIlkqPAFLCDMYSLkvdED 909  purple sea ur...
XP_022112069  924 TGTNISRSIhCMRRSVLSEKFkgmdkaskPMLLGTILHDVFDKaiegknfaAESLLKIVDRVshqLPYLKDMYAVgmtEQ 1003 crown-of-thor...
Q5ZKG3         96 SGTTISSSIrCMRKAVLSERFrgsecgsrQTLVGTILHEIFQQsvtn-nlsPEKVEELAKKIvygQKYLKEMYHLklkQT 174  chicken
PKU30560        9 RGRNINPNGqGNPSGMGSESGs------rQTLVGTILHEIFQQsvtn-nlaQEKVEELAHKIvygQKYLKEMYYLnlkQT 81   Limosa lappon...
F6QXW0        132 SGTSIANGIrCLRRSVLSEKFkvcdkgsrQMLIGTMLHDIFQRattr-gftDSVLQELAHHTvhgPKYLKEMYQLklnQT 210  tropical claw...

Feature 1                                                                                   #     
5EAN_A        209 EILCEVEEYLPSFSKWAEDFmrkgpssefPQMQLSlpsdg--snrssPCNIEVVKSLDIEESIWSprfgLKGKIDVTVGV 286  house mouse
XP_014340488  603 EVMQEVQQYLPSLTNWARNYmhnspqanqCQVQLKlpsdrklsaqdpSCNITITDFLDIEENIWSprfgLKGKMDVTASV 682  coelacanth
XP_007897282  641 EVLQEISEYVPSIIKWAEDFmhnspwpskQQLQLKlpsernlsrqdaMSSITVTDIEDIEENIWSprfgIKGKIDVTASV 720  elephant shark
TSK22614      448 DMKQELEEYLPAIAEWAKDYlhtshqtrqKQLTLRlpsdgalsrqdsECSITVTDFMDIEENIWSprfgLKGKIDVTAGV 527  goonch
XP_019639379  883 QLQEEIAEYLPVMRSWADTFyrs----rgAELEVKlpgttycnrgdeRSNVVISAVRDIEENVWAprfgLKGKLDVTTEV 958  Belcher's lan...
XP_030830072  910 NLLEEVGEYADTIKRWSDTYlg-----snPRGSVDikwpg--sfkpeNCGVSVSKVTDIEESIWSprwgVKGKVDLTVEV 982  purple sea ur...
XP_022112069 1004 DVLDDAQEYVGPMKQWADRYlqpn-pkqsATVDVKmpgq----nhseKYNVCISGVKDIEETVWSprwgTKGKVDLTVEV 1078 crown-of-thor...
Q5ZKG3        175 EIMQEIEEYLPSFFKWTEDFvrnp--anqNKMQLKlpsen--ktgdcSSTVEIVDILDIEENIWSprfgLKGKIDVTARV 250  chicken
PKU30560       82 EIMQEVEEYLPSFFKWAEDFmhsp--anqNKMQLKlssge--kkediSSKIEIVDILDIEENIWSprfgLKGKIDVTARV 157  Limosa lappon...
F6QXW0        211 DVMGEVQEYLPSFAKWAIDFmthp--lnqHQINVTrpta-----gdpTEATKVSEFLDIEENIWSprfgLKGKIDVTARV 283  tropical claw...

Feature 1                           ###            #                                              
5EAN_A        287 KIHrd---ckMKYKVMPLELKTGkesnSIEHRSQVVLYTLLSQerre-----dpeaGWLLYLk--------tGQMYPVPa 350  house mouse
XP_014340488  683 RIHrr---skTLTRVMPLELKTGkesnSIEHRSQVVLYTLLSQerra-----dpeaGFLLYLk--------sGNMYPVPg 746  coelacanth
XP_007897282  721 KIHrq---skSQTRIMPLELKTGkesnSIEHRSQVILYTLLSQerre-----dpeaGFLLYLk--------tGTVYPVPg 784  elephant shark
TSK22614      528 RIHrrg--rkHIDKIVPLELKTGkesnSIEHRSQVILYTLMSTerra-----daevGFLVYLk--------tGNLHPIVg 592  goonch
XP_019639379  959 RIQprqkgrdPLQRILPLELKTGretnSIEHRSQVILYTLLTRehhp-----dpelGLLLYLk--------tGNMKAVPa 1025 Belcher's lan...
XP_030830072  983 KIHrpsrsqpGQHANMPLELKTGketnSIEHRSQLILYSLMLGdlqp-----tsdlGFLLYIk--------tSAMKAVPa 1049 purple sea ur...
XP_022112069 1079 KIHqrnlqgrKTQMTVPLELKTGrqtnSIEHRSQLVLYSLMLDdvldg---pasdlGLLLYLk--------tGAMLAVPa 1147 crown-of-thor...
Q5ZKG3        251 KIHrq---ggIQSRIMPLELKSGkesnSVEHRSQVILYTLLNLerrv-----dpeaGFLLYLk--------tGTMYPVTg 314  chicken
PKU30560      158 KIHrq---sgVQSRIMPLELKSGkesnSIEHRSQVRILSACGFsvlltsfthtavdNILLKLakfkigflrlGRAQKVHp 234  Limosa lappon...
F6QXW0        284 KIHqk---skSHLKIMPLELKTGkesnSIEHRSQVVLYTLLSQerre-----dpeaGLLLYLk--------tGNMYSVPg 347  tropical claw...

Feature 1                               
5EAN_A        351 --nHLDKRELLKLRNWLAASLL 370  house mouse
XP_014340488  747 --nHMDRRELLKLRNQLAFYLS 766  coelacanth
XP_007897282  785 --nRMDRRELLKLRNQLAFYLA 804  elephant shark
TSK22614      593 --nHMDRRELIKLRNSLVYHLS 612  goonch
XP_019639379 1026 --gHMDRRELLLIRNRLVTQLK 1045 Belcher's lancelet
XP_030830072 1050 --nHLDKRELIHLRNQLAYYLS 1069 purple sea urchin
XP_022112069 1148 --nHMDKRELIHLRNQLAYYIS 1167 crown-of-thorns starfish
Q5ZKG3        315 --tRMDRRELIKLRNQVAFYLM 334  chicken
PKU30560      235 dirKFTEEEICKSRSIKSVTDL 256  Limosa lapponica baueri
F6QXW0        348 --nRLDRRELLKIRNELSYYLT 367  tropical clawed frog

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