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endonuclease BsaWI and similar endonucleases The type II restriction endonuclease BsaWI recognizes a degenerated sequence 5'-W|CCGGW-3', where W stands for A or T and '|' denotes the cleavage site. It belongs to a family of restriction endonucleases that recognize a conserved CCGG tetranucleotide in their target and form homodimers or homotetramers, requiring binding of one, two or three DNA targets for optimal catalytic activity. They are part of a yet larger superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many other restriction endonucleases, such as EcoRI, BamHI, and FokI. |
Jiyao W et al.(2023). "The conserved domain database in 2023.",