1D2I,1DFM


Conserved Protein Domain Family
BglII-like

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cd22312: BglII-like 
Click on image for an interactive view with Cn3D
Restriction endonuclease BglII and similar endonucleases
Restriction endonuclease BglII cleaves duplex DNA containing the sequence 5'-A|GATCT-3' (| designates the cleavage position) to generate staggered products with four nucleotide 5'-overhangs. It belongs to a superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.
Statistics
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PSSM-Id: 411716
Aligned: 12 rows
Threshold Bit Score: 286.566
Created: 28-Mar-2011
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1D2I: Bacillus subtilis restriction endonuclease BglII active site contacts substrate DNA and divalent cation; contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                #              #        # #             
1D2I_A        31 PLHVKASDQASKVGSLIFDPVGTNQYIKDELvp--kHWKNNIPIPkrFDFLGTDIDFGKRDTLVEVQFSNYPFLLNNTVR 108 Bacillus subtilis
1DFM_A        31 PLHVKASDQASKVGSLIFDPVGTNQYIKDELvp--kHWKNNIPIPkrFDFLGTDIDFGKRDTLVEVQFSNYPFLLNNTVR 108 Bacillus subtilis
Q45488        31 PLHVKASDQASKVGSLIFDPVGTNQYIKDELvp--kHWKNNIPIPkrFDFLGTDIDFGKRDTLVEVQFSNYPFLLNNTVR 108 Bacillus subtilis
WP_017305696  34 PLYVKGSNQQGIKGNMVFDPIATNAYIKNSLqs--kGWIANAAIPehINPLGKDVDFTKNTVLLEIQFSHYAFLLNNIIR 111 Spirulina subsalsa
WP_027255123  34 PLHVKASSQAGIEGNPVFDPVGTNEYIKAELvk--lGWQANIPIPtkYRFLGKDIDFGKASAIIEVQFSHYAFLLNNTIR 111 Planktothrix ag...
WP_061362789  34 PLHLKASDQANIQGRAIFDPVGTNQHIADGLvp--lGWQQKIVIPqdFKFLGTDVDFGKNGTVVEVQFSNYPFLLNNTLR 111 Pseudomonas aer...
OCQ92689      36 PLHIKPSDQANIKGNPIFDPVGSNQYIKNTLvk--lGWHSNILIPveYRFLGKDVDFGKSGILLESQFSNYPFLLNNLLR 113 Nostoc sp. MBR 210
ODU11079      34 PLHLKASDQAGIQGKAIFDPVGTNQHIADQLttaprSWAGGIPIPaeFSFLGTDIDFGKNGVVVEVQFSNYPFLLNNTVR 113 Rubrivivax sp. ...
WP_088243598  34 NLHIKASDQAGKIGSIIFDPVGTNSDLKDGLiv--kSWQDNPKIPvaFRFLGKDIDFEKNGLIVEVQFSNYPFLLNNILR 111 Calothrix rhizo...
WP_096571747   2 PLHIKASSQANKKGEPIFDPVGTNLYITNYLnk--sGWNVLPIPNq-YKFFGKEIDLAKAGLVIENQFSNYPFLLNNTAR 78  Sphaerospermops...
PID41508      34 PLHLKASDQAGLKGQAIFDPVGTNAYIKSKMtg--cGWGSNVNIPneFRFLGKDIDFVKRGLLVEVQFSNYPFLMNNIQR 111 Proteobacteria ...
RDI46151      34 SLQLQPSDQAGKGHRPIFDPKATNRELTDAAal--hGWKSIPVPKe-LQMFGSDWDVGKQTTLVEWQFSNYPFLWNNIIR 110 Nocardia mexicana
Feature 1                                                                                        
1D2I_A       109 SELFHKSNxdid-eegXKVAIIITKGHXFPASNSSLYYEQAQNQLNSLaeYNVFDVPIRLVGLIedf-etDIDIVSTTYA 186 Bacillus subtilis
1DFM_A       109 SELFHKSNxdid-eegXKVAIIITKGHXFPASNSSLYYEQAQNQLNSLaeYNVFDVPIRLVGLIedf-etDIDIVSTTYA 186 Bacillus subtilis
Q45488       109 SELFHKSNmdid-eegMKVAIIITKGHMFPASNSSLYYEQAQNQLNSLaeYNVFDVPIRLVGLIedf-etDIDIVSTTYA 186 Bacillus subtilis
WP_017305696 112 SEVFFKSRsaln-shsVALMVLVTKFDILPSANSSLHYTQAVEQLLVIetANIFSLPTRIVGLNvdi-enEIQAIFTQYQ 189 Spirulina subsalsa
WP_027255123 112 SELLFNTQlsvt-gipMQILIIIAKSRMFPAGNSSLYYEQAVNQLNLLa-GYVFNVPIRVVGLFelk-naTVPAKRTIYK 188 Planktothrix ag...
WP_061362789 112 SELFFRAQtvfh-gapTGLVIIVTKAGMFPSSNSTLYYEQALNQLTALaqHGVFTVPIRLVGLFen--vgRVQATWTGYS 188 Pseudomonas aer...
OCQ92689     114 SELFFKSRihfa-gnsTKLLVIITKAQMFPASNSTLYYEQAVQQLTALikHHVFDIPIRLIGLFeqk-ntTISAVSTIYQ 191 Nostoc sp. MBR 210
ODU11079     114 SELFFRAQtvfh-arpTQLVVIVTKAGMFPSSQSTLYYEQALNQLTALaqHGVFTVPIRLVGLFtp--vgHVSATWTEYS 190 Rubrivivax sp. ...
WP_088243598 112 SELFFKSKtkfg-anpTKALIIITKAGGFPSSNSTLYYEQAVKQLNSLhsYGIFSVPTRLVGLFpei-daVNDVTWTEYE 189 Calothrix rhizo...
WP_096571747  79 VEFLFQKQilla-gnpIKLIVIITKSQVFPSANSSLYYEQAVKQLLALdeIALFKIPIRVVGLFent-ntITSAIWTEYG 156 Sphaerospermops...
PID41508     112 SDLFFKSEtvlg-rskAELLIIVAKAHMFPASQSTLYYEQAINQLDALvqHGGFSIPVRLAGLEspm-grNIPSIWTTYD 189 Proteobacteria ...
RDI46151     111 TQGVYRSKiqlsgmsgVEAMIIVTKSGKLPASNSTLYYEQARAQIKTVmdFLEFTLPIRLVGITldslarSVEAVWNTYA 190 Nocardia mexicana
Feature 1                              
1D2I_A       187 DkRYSRTITKRDTVKGKVIDTN 208 Bacillus subtilis
1DFM_A       187 DkRYSRTITKRDTVKGKVIDTN 208 Bacillus subtilis
Q45488       187 DkRYSRTITKRDTVKGKVIDTN 208 Bacillus subtilis
WP_017305696 190 SpQSRRIESQTPINCMVQKSGK 211 Spirulina subsalsa
WP_027255123 189 D-KTSRTILTQEERQCQIISGR 209 Planktothrix agardhii
WP_061362789 189 AaRYSRTVGSRELRPFVITNGR 210 Pseudomonas aeruginosa
OCQ92689     192 StRYSRIVNTQIDCQCQILPSH 213 Nostoc sp. MBR 210
ODU11079     191 AaRYSRTVGSRSQRQFTIINGR 212 Rubrivivax sp. SCN 71-131
WP_088243598 190 NaRYSRTPVKEETKKYKITNGT 211 Calothrix rhizosoleniae
WP_096571747 157 K-TTARNIVTQYQRQYQIIPGR 177 Sphaerospermopsis kisseleviana
PID41508     190 NpRYSRKVIQQEKVPVDIAPGN 211 Proteobacteria bacterium
RDI46151     191 NnRYDRVPRSKVLRTFDVTWSS 212 Nocardia mexicana

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