1DMU


Conserved Protein Domain Family
BglI-like
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cd22311: BglI-like 
Restriction endonuclease BglI and similar endonucleases
BglI is a type II restriction endonuclease that recognizes the interrupted DNA sequence GCCNNNNNGGC and cleaves between the fourth and fifth unspecified base pair to produce overhanging ends; it belongs to a superfamily of nucleases including very short patch repair (Vsr) Endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.
Links
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Statistics
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PSSM-Id: 411715
Aligned: 14 rows
Threshold Bit Score: 386.685
Created: 28-Mar-2011
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
Download Cn3D
 
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1DMU: Bacillus subtilis restriction endonuclease BglI active site contacts substrate DNA; contacts at 4A
    - View structure with Cn3D
  • Citation:PMID 9736624
Download Cn3D for Viewing 3D StructureScroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                        
1DMU_A        10 FMSYNQNKQYLEdn-pEIQEKIELYGLNLLNEVISDNe--EEIRADYNEANFLHPFWMNYPPLDRGKMPKGDQIPWIEVG 86  Bacillus subtilis
WP_018399041  10 YSLYNQARTHFLqh-yNQLINIEKFVSTKIDEIINKNl--QQIVNDFNEASNLYPFWQNYPPDNRGRAPIGDQYPWIEVG 86  filamentous cya...
REJ51572      10 FQLYNSARNYFIas-pNTLIELERFLTNHIVSLVKSNl--AEIKNDYNEASYLYPFWENYPPGDRGRQPIMDQYPWLEVG 86  Microcystis aer...
WP_074928903  10 FNIYNSARNHFIan-pSSLIELEKFLTNYLVSIITANi--VEIKQDYNEASYLYPFWENYPPEDRGRQPIKDQYPWIEVG 86  Nitrosomonas eu...
GBR74484      10 FAQYNKAHDFFIen-hEKLIQIEHFCMDLLSKFIDEHq--DSIKHDYNEASFLYPFWQNYPPDERGRKPKGDQYPWIEIG 86  Candidatus Marg...
WP_107898046  10 FDLYQRAVDFYKkn-yKNLINIEYFIMKEICHFIDLYl--PEIQRDYNEASYLYSFWKNYPPLDRGRSPKGDQYPWIEVG 86  Campylobacter c...
WP_027003281   1 MKNYNSIRQSLIqd-iSQIINLEKIYTESILKGLCDNa--LKIKQDFDKTIALRDFWFRYAPRQRGHKPSGKSLPWGEVG 77  Hugenholtzia ro...
WP_028666517   6 SDQYFLSREKLSnn-lQQIIDTERYYTQFLLDEIILPvaeDIWSNFTLDPLKLLPFWRNYPPEQRGRGPIGTSVPWLELG 84  Runella zeae
STY53548       3 LYDYHDLRAGLLandcHGILSLEQRTTETLVRAISSQa--ANIKRDFDKAFNLMPFWSAYAPVQRGHMPRGEAFPWGEVG 80  Vibrio anguillarum
WP_042114997   6 INNYLAARQYIInnnsNFILQEECRVTDLIIDRLASRy--REIKNDFDNAYRTKEYWELYAPIQRGHKPRGEAYPWGEVG 83  Escherichia coli

Feature 1        #                            #                         # #                      
1DMU_A        87 EKAVGSKLTRLVSqreditVREIGLPTGPDERYLLTsptiysltngftdSIMMFVDIKSVGPRDSDYDLVLSPNQVSGNG 166 Bacillus subtilis
WP_018399041  87 EHTIGEKLPRLLEpy--fhIRDMGLPSGTDVRLVLTnaeinkltnrltdTCWLFLDIKSVGPRDDQNHAVMSPNQISGNG 164 filamentous cya...
REJ51572      87 EHTIGAKLPRLLVns--fdVRDTGIPTGADQRFVISsknileatqgftnSAWLFIDIKSVGPRDDQDHTVMSHNQVSGDG 164 Microcystis aer...
WP_074928903  87 EHAIGSKLPRLLDsv--frVRDTGLPTGSDQRFVLTddaiatatggftnSVWFFVDIKSVGPRDDQHHTVMSHNQVSGDG 164 Nitrosomonas eu...
GBR74484      87 EHVFCPKISRFLDkh--fnIKDTGLPTGPDGRYIISndaiqs-ileitcSVWLFVDIKSVGPRDDQDHVVMSHNQISGNG 163 Candidatus Marg...
WP_107898046  87 EQVFGNKLLRYFSan--frIKDSGIPSGSDDRCIISspkieq-ilnftdSIWVFVDIKSAGPRDDFNHAVMSPNQISGSG 163 Campylobacter c...
WP_027003281  78 EKVLDAYLYKIIVeif-dnIRFVGLPYGHDIRFVSE-------------SAFVQMDIKSTGPTDNTDEVVSSPNQVSGDG 143 Hugenholtzia ro...
WP_028666517  85 EKTISSNIIKALGskr-dnITFPGLPTGGDIRFADK-------------QVYIHLDVKLTGPNDNANEVVVPPNQVSGDG 150 Runella zeae
STY53548      81 EKVVEGYLYSLLPqvf-neVAFPGIPYGHDVRFSTL-------------DAFIHIDAKSTGPTDNLNEVVSSPNQVTGDG 146 Vibrio anguillarum
WP_042114997  84 EKVLEGHLYSSLNeif-erVKFPGIPYGHDIRFSTE-------------NSFVHIDIKSTGPTDNPNEVVSSPNQVTGNG 149 Escherichia coli

Feature 1                                                                                        
1DMU_A       167 DWaq-leGGIQNNQQTIQGPRSSQIFLPTIPPLYILSdgtiaPVVHLFIKPIYAMRslt---kgDTGQSLYKIKLASVPN 242 Bacillus subtilis
WP_018399041 165 RWda-edNGVVNDVIVAKGKRKSQDFYCSIPPIYVLSdgtilPVIILIVKPVYRMLslee-nskDGGQPLGRISLASVPN 242 filamentous cya...
REJ51572     165 TWen-sqAGVRNSILQVIGARASHDFQASIPPIYVLSdgtiaPVVIIALKPVYQMLqanhsnirNNGQPLERIDVACIPN 243 Microcystis aer...
WP_074928903 165 VWin-pvDGVRNTILQATGARASHDFHASLPPVFVLSdgtiaPLVMIALKPVYRMLqpnvvgarNDGQPLERIDIACIPN 243 Nitrosomonas eu...
GBR74484     164 KWdr-maCGVLNDTMVAKGQRATHPFYCAIPPLFVLSdatvvPVVHIVLKPVYKMLgla---geRQGQPIGRVSMATIPN 239 Candidatus Marg...
WP_107898046 164 NWna-idIGLINNNIVAKGNRKAHNFSCSLSPIYVLSdgtvaPLITFVIKPVYKMIfdk---gnNIGQPIHKIKIASIPN 239 Campylobacter c...
WP_027003281 144 LFid--eNGVFNSKVLVQGSSKAMEFQPELPPFYLIDnk-vfITLTFYLKCVYKVId-------LGNQPLDYLEMICVPN 213 Hugenholtzia ro...
WP_028666517 151 INwndgvLNSTWPVRRVKSGEINYHFQPKLPPIYILDdn-tlICLTYFFDAIYDVTg-------FGNHPIKYFELVCVPN 222 Runella zeae
STY53548     147 AIfd--gGQVRNNITQMRGARVSRDFQPELAPFVVDNgv-vkPVLTYYLKIAYTVSa-------PGNQPLWYLELICVPN 216 Vibrio anguillarum
WP_042114997 150 MIse--aGMVYNNEVDMRGARITRKFRPELAPFYLINnq-iiPVLTFYIKVVYDVIs-------PGNQPLKYIELICVPN 219 Escherichia coli

Feature 1                                                               
1DMU_A       243 GLglfcnpgyafdsayKFLFRPGKDDRTKsllQKRVRVDLRvl-dKIGPRVMTID 296 Bacillus subtilis
WP_018399041 243 GIllqenp--nylqqyPNLFFPGKDDQSTnplKKRCRISFDvlksIEAWRFKEIV 295 filamentous cyanobacterium ESFC-1
REJ51572     244 GLlltqnp--nylntyRGILFPGKDDKSKdprKLRVRVSFSllkkIHPWRVESIL 296 Microcystis aeruginosa TA09
WP_074928903 244 GLlltqqp--nylgayNGLLFPGKDDKSKdprKLRARVSFEllknIAPWRVQTIQ 296 Nitrosomonas eutropha
GBR74484     240 GIlltenp--nylkktPGLLFPGKDDKEKdprKIRARVSFEklrkLADWRYKDFY 292 Candidatus Margulisbacteria bacterium Nk...
WP_107898046 240 GIllthqp--nylkkyPQLFFPGKDDKNKdtrKIRARVSFDilkqIDNWRVSEVE 292 Campylobacter concisus
WP_027003281 214 GLlmfdsl--hyakntKGLLTPGKDILTSahkRTRIKLNPLa--qIAKWRCQKIV 264 Hugenholtzia roseola
WP_028666517 223 GLlmfdgp---klantPNLIIAGKDDKTTpddAKRIRIRLEplskINNWRATKIK 274 Runella zeae
STY53548     217 GLmlfaedglnlvgrvQGMLTPGKDEQHVa--RKRTRIKLDplsqLAQWRCTKIF 269 Vibrio anguillarum
WP_042114997 220 GMplfvap--nfnylyPGILTPGKDEVGVdrkRTRIKLNPLa--kIGSWRCQKIY 270 Escherichia coli

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