3IMB


Conserved Protein Domain Family
BcnI-like
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cd22310: BcnI-like 
Restriction endonuclease BcnI and similar endonucleases
Restriction endonuclease BcnI cleaves duplex DNA containing the sequence 5'-CC|SGG-3' (S stands for C or G, | designates a cleavage position) to generate staggered products with single nucleotide 5'-overhangs. It belongs to a superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.
Links
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Statistics
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PSSM-Id: 411714
Aligned: 24 rows
Threshold Bit Score: 251.785
Created: 28-Mar-2011
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
Download Cn3D
 
active siteDNA binding
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:3IMB: Brevibacillus centrosporus restriction endonuclease BcnI active site contacts substrate DNA; contacts at 4A
    - View structure with Cn3D
Download Cn3D for Viewing 3D StructureScroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                           #             #     # #                      
3IMB_A         6 KEEVVNKLHEIKNKGYLSVptdmfRTDDGVVGQILERQFGVQENNITLGDL-GEFELKGMRNRKak-sNLTLFHKKPVAG 83  Brevibacillus c...
KIU10063       7 TEEIIKKLEYIKEQGFIGIpevdfRSDDGVVGQVLERAFGVSENNLRIGDL-GIFELKAMRNRKnk-sNLTLGHQKPTEG 84  Bacillus subtilis
SCI72438       7 KEAVIERLKEINSKGFISVpkgmfRADDGIVGQILEREFGVAENNLHLADL-GTYELKGMRKKKgktsKLTLFHQTSTSG 85  uncultured Buty...
WP_004128419   5 KESVIKKMAEIRDKGFIRIpddmyRKDDGIVGQILEREFGVAENNLHLADL-GTYELKGMRKKNka-sTLTLFHKTSTSG 82  Gardnerella vag...
WP_117733557  15 DDTFQSAMQGVEQKGFIPSh----RAGDTGIGKTLEDELGIEENPIQAADL-GSVELKATRKDSg--sSLTLFTKSPAKR 87  Ruminococcus sp...
WP_006306723  15 DDKFKEAIEQVEKKGFIPSh----RAGDTGVGKTLEDELGVVENSVQAADL-GEVELKATRRDSn--sKLTLFTKAPKKR 87  Selenomonadaceae
WP_073197853   4 LETLKQRLKEIEQMGYIPTh----RSGPTGIGKTLEDLLGINENNIAGADLgDLGELKSCRNNQt--sMITLFTKSPKPP 77  Caloranaerobact...
WP_111675306 152 LSRLIPKVKEIASKGYHPNskgigKISPKDAGDTLENLLGIKTNNSQKADFeDLIELKSKTSKT----LDTLFTLRPKFE 227 Streptococcus s...
PIS34799       3 LLTLRKELQKIKKFGFVPTh----RTGDTGIGKTLEDLLNIKENNIPLHDIaGVAELKACRRSAk--sMLTLFTLEPLPK 76  Parcubacteria g...
WP_044825221   4 LERLKIRLREINQMGYVRTh----RSGPTGIGKTLEDLLGIAENNIAGADLdHLGELKSCRNGQi--sMVTLFTKSPSPP 77  Clostridium ace...

Feature 1                                                                                        
3IMB_A        84 QTVIQIFn------------rFGYVKPSSRnpevmkKKLFTTIKGGRL-----NNLGLTLNAKha---sEINLYYq---d 140 Brevibacillus c...
KIU10063      85 MSTRQLFe------------kYGYIKESTRnpgimkKKLFTTIKGSRY-----NNRGFILKVRdgn-dgIIDLYHkt--d 144 Bacillus subtilis
SCI72438      86 MTPRQIFn------------rFSYEKPSNRdds-lkRKLFTTIYGNKF-----NRLGFTLRAQgv---sEVCLYHe---e 141 uncultured Buty...
WP_004128419  83 LSPNEIFd------------rFSYERPSQRdgt-pkRKLFTTIRGDKR-----NSLEFILRGNge---aNVDLYYr---d 138 Gardnerella vag...
WP_117733557  88 GVNNSVLrn-----------qYGYQTEESMelnpnaKVLHTTVNGAGFnt-lnGEEFMKLTVQd----dRLYLEHkqdgi 151 Ruminococcus sp...
WP_006306723  88 GVNSRVLrs-----------rYGYQTDESRalnpniNILHTQVNGRDFnt-ldGEPFLKLTAQd----dRLYLEHakdgi 151 Selenomonadaceae
WP_073197853  78 RVNGILLn------------tYGYVDPRRDs----rKILHSTVSGTSYntvngTPYGFKVEVVg----dRLYLKSnf-pq 136 Caloranaerobact...
WP_111675306 228 GTPVAEFesndrnrvsafarlYGYESDKHPg----mNSLYITIGSEMSp---qNNQNFYLDVNesdrtvEIRRHEnd-ts 299 Streptococcus s...
PIS34799      77 GGDRDRVll----------dnFGYSKRNNRr----sKELHSTLSCKRY-----NNQSLKLSVSg----dKIRVQGkg--k 131 Parcubacteria g...
WP_044825221  78 RVNTALLe------------sYGYVDPTRGg----rKILHTTLNGVNYntvngTPYGFKVEVRg----sRLYLLSnf-pt 136 Clostridium ace...

Feature 1                                                                                        
3IMB_A       141 EYLSTWDLNLs--------KIEKLVLVFAETIGranspeEQFHFTKAYMLTEIndits--lindGVLVMDLCIDQdlsk- 209 Brevibacillus c...
KIU10063     145 GYLSTWVLDLk--------KVENMVLVFADTEGkpnardEKFHFVEGYLYKNMnslnn--lvndGYLVMDLCIDQpvdg- 213 Bacillus subtilis
SCI72438     142 EYLATWDLTGgk------gKINQVLFVLAETQGpanseyERFHFIEAYILSSPkniyd--aissGAVVMELCIDQpanef 213 uncultured Buty...
WP_004128419 139 EYLATWDLTSgn------gKIKQVLLALAETRGaansqnEEFHFVEAYILSSPrniyd--aitsGDVVMDLCIDQpaadf 210 Gardnerella vag...
WP_117733557 152 LEDVYWEEEDlrkafakkyPAGKLYHVQADVRVedd-gsESFHYDEAISLSGFsadrmieglqsGELEMDIRLGVyasgk 230 Ruminococcus sp...
WP_006306723 152 LEDVYWEQEQlkkafdkkyPSQKLYHVQAEVKKedd-gsESFHYSEAYSLGDFnaermieglksGELDVDIRLGVyasga 230 Selenomonadaceae
WP_073197853 137 QVTAYWEKEDlkka--fekKLPKLIFVKANCKGtg--rnEEFHFVEAYQLSGFsfknfeelinrGIIKIDIRIGQypd-- 210 Caloranaerobact...
WP_111675306 300 QRVAYWYFEDleke--lhlKHPATLWVKAESKMng--diAEFKYTEAELTRSPqfmtfisliklGVVTYDWRGYTskeg- 374 Streptococcus s...
PIS34799     132 RLNIYWDTESvqkk--fgnKLPALVYVLADTKEek--gkEYFHFNEAYLLSGFdfdlfqkmvkkDQIVVDFRMYYrpn-- 205 Parcubacteria g...
WP_044825221 137 QVNAYWEREDlrya--fesKLPRLIFVKANSRGag--rnEEFHFVEAYHLEGFsfeqfedlleqGIIKIDIRIGQypd-- 210 Clostridium ace...

Feature 1                                      
3IMB_A       210 -skgPHDRGPHLRIPISKLDKLYRNIERLL 238 Brevibacillus centrosporus
KIU10063     214 -sktMHDRGPHWRISTKQLPKLYKEVHQLL 242 Bacillus subtilis
SCI72438     214 gkkaPHDRGPHIRIPVKKLSTLFESVERVL 243 uncultured Butyricicoccus sp.
WP_004128419 211 rqkaPHDRGPHIRIPVRRLNSLFESVEKII 240 Gardnerella vaginalis
WP_117733557 231 kkgkLHDNGTAIRVSPRKLDDCFDEKKELL 260 Ruminococcus sp. OM06-36AC
WP_006306723 231 rkgkPHDNGTAIRVSSKKLDECFDEKKKLL 260 Selenomonadaceae
WP_073197853 211 --grTHDHGTAFRIMNNQIHLCFAHRIKLL 238 Caloranaerobacter azorensis
WP_111675306 375 -kyeGKNHGNAWRVKNKHRNQLFGSSEVIE 403 Streptococcus sanguinis
PIS34799     206 --gsVRNHGTGFRIKINKLYNCFRNKDQLI 233 Parcubacteria group bacterium CG08_land_8_20_14_0_20_43_9
WP_044825221 211 --grTHDHGTAFRIMNDRIDDLFENKIRLL 238 Clostridium aceticum

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