5XBU,5XCA,1WC2,5XC8,3X2J,3X2G,3X2L,3X2O,4ZM7,1L8F,5GLX,6MVJ,5H4U,3ENG


Conserved Protein Domain Family
DPBB_GH45_endoglucanase
?
cd22278: DPBB_GH45_endoglucanase 
Click on image for an interactive view with Cn3D
double-psi beta-barrel fold of glycoside hydrolase family 45 endoglucanase EG27II and similar proteins
This group is made up of endoglucanases from mollusks similar to Ampullaria crossean endoglucanase EG27II, a glycoside hydrolase family 45 (GH45) subfamily B protein. Endoglucanases (EC 3.2.1.4) catalyze the endohydrolysis of (1-4)-beta-D-glucosidic linkages in cellulose, lichenin, and cereal beta-D-glucans. Animal cellulases, such as endoglucanase EG27II, have great potential for industrial applications such as bioethanol production. GH45 endoglucanases from mollusks adopt a double-psi beta-barrel (DPBB) fold.
Links
?
Statistics
?
PSSM-Id: 439258
Aligned: 25 rows
Threshold Bit Score: 78.2884
Created: 31-Jul-2020
Updated: 25-Oct-2021
Structure
?
Program:
Drawing:
Aligned Rows:
Download Cn3D
 
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:chemical substrate binding site [chemical binding site]
Evidence:
  • Structure:5XC8: Ampullaria crossean endoglucanase EG27II binds two molecules of cellobiose; contacts at 4A
  • Structure:5XCA: Ampullaria crossean endo-beta-1,4-glucanase in complex with cellobiose, contacts at 4A
  • Structure:6MVJ: Neurospora crassa endoglucanase V in complex with cellobiose, contacts at 4A
  • Structure:3ENG: Humicola insolens endoglucanase V in complex with cellobiose, contacts at 4A
  • Citation:PMID 8377830
Scroll to Sequence Alignment Display
Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection: 
Feature 1          ### #     ####                                                        #    
5XBU_A     23 PCASTTRYVDg----HKGACGCGQKgsdtpf-----------------------------------pwnlqkHVTAPSEr 63  Ampullaria crossean
5XC8_A     23 PCASTTRYVDg----HKGACGCGQKgsdtpf-----------------------------------pwnlqkHVTAPSEr 63  Ampullaria crossean
3X2J_A     11 GQASFTMYSGc----GSPACGKAASg----------------------------------------------FTAAINQl 40  Phanerochaete chry...
3X2G_A     11 GQASFTMYSGc----GSPACGKAASg----------------------------------------------FTAAINQl 40  Phanerochaete chry...
3X2L_A     11 GQASFTMYSGc----GSPACGKAASg----------------------------------------------FTAAINQl 40  Phanerochaete chry...
3X2O_A     11 GQASFTMYSGc----GSPACGKAASg----------------------------------------------FTAAINQl 40  Phanerochaete chry...
4ZM7_A     11 GQASFTMYSGc----GSPACGKAASg----------------------------------------------FTAAINQl 40  Phanerochaete chry...
5H4U_A     20 GSGVTTRYWDc----CKPSCSWGGKasvtkpvrtckangnttidsntqsgcnggssyvcndqqpftqgnvgyGFAAASIs 95  Cryptopygus antarc...
GAO47054  382 LNGSCTWYTGc---sSQNGCGKQPLs---------------------------------------------gYTAALSSf 413 Saitoella complica...
PMD28029   33 GTASTTQFYLgtelsSGTACGVDALpngkstsg-------------------------------kqgggpgyLYAAINQl 81  Pezoloma ericae

Feature 1             ##                                    ####                          # # 
5XBU_A     64 yfddggsnLWCGKNCGKCVRLTPTggfvpgkggappnHNPVVFMVTNACPingneewcgisgkpgtnhvnshgyevHFDL 143 Ampullaria crossean
5XC8_A     64 yfddggsnLWCGKNCGKCVRLTPTggfvpgkggappnHNPVVFMVTNACPingneewcgisgkpgtnhvnshgyevHFDL 143 Ampullaria crossean
3X2J_A     41 afg-sapgLGAGDACGRCFALTGNhdpysp-nytgpfGQTIVVKVTDLCPvqgnqefcgqtt---snptnqhgmpfHFNI 115 Phanerochaete chry...
3X2G_A     41 afg-sapgLGAGDACGRCFALTGNhdpysp-nytgpfGQTIVVKVTDLCPvqgdqefcgqtt---snptnqhgmpfHFDI 115 Phanerochaete chry...
3X2L_A     41 afg-sapgLGAGDACGRCFALTGNhdpysp-nytgpfGQTIVVKVTDLCPvqgnqefcgqtt---snptnqhgmpfHFDI 115 Phanerochaete chry...
3X2O_A     41 afg-sapgLGAGDACGRCFALTGNhdpysp-nytgpfGQTIVVKVTDLCPvqgxqefcgqtt---snptnqhgmpfHFDI 115 Phanerochaete chry...
4ZM7_A     41 afg-sapgLGAGDACGRCFALTGNhdpysp-nytgpfGQTIVVKVTDLCPvqgnqefcgqtt---snptdqhgmpfHFDI 115 Phanerochaete chry...
5H4U_A     96 -------gQPESQTCCACYEMTFTnta--------isGQKMIVQVTNTGSdln---------------------gnHFDL 139 Cryptopygus antarc...
GAO47054  414 lf---gsaQGAGSACGRCFQLQGYedpyqp--gvelgTPAVIVKVTDKCPagssdplcsqtp---eeplnqygalaNFDL 485 Saitoella complica...
PMD28029   82 af---adaGGPGGACGICYQLTPVsssg-----tvlsSQALTFMIVDECPaaealsggyncdqctisetnvlgqhwNFDI 153 Pezoloma ericae

Feature 1                                                                #          
5XBU_A    144 QDQVGQVea----------------------------------------------lhWDNPEVTWEEVPC 167 Ampullaria crossean
5XC8_A    144 QDQVGQVea----------------------------------------------lhWDNPEVTWEEVPC 167 Ampullaria crossean
3X2J_A    116 CEDTGGSakff-------------------------------------------psgHGALTGTFTEVSC 142 Phanerochaete chrysosporium
3X2G_A    116 CEDTGGSakff-------------------------------------------psgHGALTGTFTEVSC 142 Phanerochaete chrysosporium
3X2L_A    116 CEDTGGSakff-------------------------------------------psgHGALTGTFTEVSC 142 Phanerochaete chrysosporium
3X2O_A    116 CEDTGGSakff-------------------------------------------psgHGALTGTFTEVSC 142 Phanerochaete chrysosporium
4ZM7_A    116 CEDTGGSakff-------------------------------------------psgHGALTGTFTEVSC 142 Phanerochaete chrysosporium
5H4U_A    140 MIPGGGVgifngcqsqwgapsngwgqryggissqsecnqlptslragcnwrfgwfknADNPSMKFTQVRC 209 Cryptopygus antarcticus
GAO47054  486 CRDTGAYeaff-------------------------------------------tpaHDMVLCSWFEVDC 512 Saitoella complicata NRRL Y-...
PMD28029  154 AIDAMSTsqydtff-------------------------------------ngvtdgSNWLEVQFSHVSC 186 Pezoloma ericae

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap