Conserved Protein Domain Family
asqI-like

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cd22187: asqI-like 
protein asqI and similar proteins
This family includes Aspergillus nidulans tyrosinase family protein asqI (aspoquinolone biosynthesis protein I) that is part of the gene cluster that mediates the biosynthesis of the aspoquinolone mycotoxins.
Statistics
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PSSM-Id: 409194
Aligned: 12 rows
Threshold Bit Score: 346.676
Created: 14-Jul-2020
Updated: 25-Oct-2021
Structure
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Aligned Rows:
 
putative
Feature 1: putative catalytic site [active site], 4 residue positions
Conserved feature residue pattern:x [LW] Y DClick to see conserved feature residue pattern help
Evidence:
  • Comment:alanine mutants at these positions were found to be completely inactive, suggesting their direct involvement in the catalytic reaction
  • Comment:these residues may be involved in an acid-base-type hydration

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                         
Q5AR52        716 PFRLFNGTSSVEEISQQDQMDAPkimphVNATTFEWWYFDAvsTTSQNESLTVMFENMGpeglg--apypGGPLAVQISG 793  Aspergillus n...
WP_154073609   16 ADYARLGIDPVSVVPFEDGQRIG-----TEKGRYEWWYFDA--HLDDGATVVVVFYTKPnvs-----pngPLAPRITINL 83   Bradyrhizobiu...
WP_147635443   29 EHYEKLGLIKGQIAPWEDGMRTD-----GSAGTYEWWYFDS--HLNDGSKLVIVFYTKPmme-----idsPLTPYVSFTW 96   Risungbinella...
WP_125715604   13 EDYEYWGVKPNRVEPWEDGMRTK-----PEDKSFEWWYLDA--SMDDGSSVVLTFMTKDamn-----fgtELKPWVSLEW 80   Lactobacillus...
WP_048687006   19 KDYQQLGIDPVIVKSFEDGMRMD-----GAEGTYEWWYFDA--HLDNGAKMVVVFFTKPytm-----phlPLSPFIRLNI 86   Bacillus
OOS02358       18 EDYQRLGIDPMVVQPFEDGMRIEg----ASEGTWEWWYFDA--HLNNGAKMVVVFYTKPntm-----phlPLSPFIRISL 86   Moraxella cun...
SUB53115       14 RDYQALGLSQGTVEAWEDAFRTAe----GGPGTYEWWYFDA--HLNDGSILVIIFYTKGfne-----sraKLRPKVSFRW 82   Nocardia bras...
WP_094094517   24 EHYKRLGIRKNDIKPWEDGYRTK-----DVKGTYEWWYFDS--QMDDGSTLVIVFYSNKwtla----pkkGPTPQVTFAL 92   Paenibacillus...
PSR72288       33 PGQTSQGPSTVQFVSEQHGFDGPkv-ksLNSTTSDWWYFDVv-SSNLDASVVIAFFSAEstglwegipdlGTATWAYTII 110  Phlebia centr...
OBT70374       26 PNTIKTGPSTALYVSGESGFDGPkmspgVNATTFDWWYFDAv-STDGSAAVVMVFYLCTdlgfp--fvppLSTLSVDIFA 102  Pseudogymnoas...
PNP78982       31 SPILSPGPSDVLFTSCASGADGPrv-ypINSTTFDWWYFDAv-SDDGEHALTVIFFTSSfvgfs--fdllNAIDPLNVYV 106  Fusarium nygamai
CVK95656       14 QSLQKLGLDRSQPVSWEDGLRSPg----VESGEWEWWYADA--HLQDGWFLQVAFFYITeg-------dgPKTGLIDLNI 80   Fusarium mang...
Feature 1                                                                                         
Q5AR52        794 SFSNGTAfti----itaATKGAVLewgGGGVRGEWRGAGSSFa---gKDHREYTVSIANPGigywSQFNPKCLKVSPPRY 866  Aspergillus n...
WP_154073609   84 TLPDGRTfek-----flDTKPELFs--ASKSGCDVRIGANRFv----GDLHRYYITATIEE----ISVDIELTGEVPAWR 148  Bradyrhizobiu...
WP_147635443   97 DLPDGRTiqr----tyaATSEKNFf--ASTEQCEVRIGANVFk----GNLHDYQIHIEMDE----LVADISLSGKVPPWR 162  Risungbinella...
WP_125715604   81 TDQSGQTtty-----rvDVSATAFe--AATDHCKVKIGQCTLe----GDLKHYEVHFDNAQ----IQADFTFDNLLPSWR 145  Lactobacillus...
WP_048687006   87 ELPDGRSiqk-----ikTFDAKDFs--SSRETCDVKIGDNRFy----GDLQHYSIQAELDE----VSVDVQLEGQISPWR 151  Bacillus
OOS02358       87 ELPDGRSlqk-----sqMFPAEVFs--SSTECCDVKIANNHFy----GDLNSYTIQASIDD----VAVEVQLQGQIRPWR 151  Moraxella cun...
SUB53115       83 DRPDGTRlkh-----siATSAQEFy--ADVDNCDVCIGPAKMs----AAVDEYRLTIHDGD----FRAVITLARDTPSWR 147  Nocardia bras...
WP_094094517   93 HTADGQEfse-----tmEMPFSSFe--AAVDHCRVKIEDCLFe----GDLHEYHIRFNNGK----IEANVRLAGNVPSWR 157  Paenibacillus...
PSR72288      111 TFPNGTQsvi-----ffPVDQMTV---VTVENGGYATMGNQTgwagtPDMSEYWVWVDAPDa--gIEGNLVLKSVSDSKV 180  Phlebia centr...
OBT70374      103 TFDDGSLvflplnnlpyKAGQATIvtdGDGASGNWQGTGFSFeg--sADLSTYKVVVDSPVl--aIKGTLELKSNAPAHY 178  Pseudogymnoas...
PNP78982      107 FYNNGGDgfs----fpiASTSVTIkldGDGATGDWTGSGAKFeg--aADLSTYKVTFKKTLlnpsVEGTFTLKSRGPGHY 180  Fusarium nygamai
CVK95656       81 AKDGKKLcdlh--krigNVPSETV---VARDRCDLQYRKNYFrs--vNGLSEYHIFVDPDEme-gFGFDVVIKRTTPSYR 152  Fusarium mang...
Feature 1                                # #                          # #                         
Q5AR52        867 PCDVKerr-------asEQLIPNMYLANSQPDAiVEVDFNINEStlKFSGIGYHDLSWGSAPLESSVHSWYWGHGRLGRY 939  Aspergillus n...
WP_154073609  149 PKSGHlyf-------gtEGREKLFAWLPSVPHGrASVRYTIGPEehRVSGSGYHDHNWGDVPMQTLMHNWYWARASVGPY 221  Bradyrhizobiu...
WP_147635443  163 PETGYwl--------ygEQDEHYFAWLPSVPQGkVEAMIQMGEDyrQYTGMGYHDHNWGNIAMPALMNNWYWGRAQVGDY 234  Risungbinella...
WP_125715604  146 PAAGAn-----------LFGDHYFAWLPSVPAArVNATIELDGQtsTLTGTGYHDHNWGNESMLKLMHHWYWGRAAVGDY 214  Lactobacillus...
WP_048687006  152 PKSGHmyy-------geKESEQLFAWLPSVPQGkVSATFTVGDEkfSSEGVGYHDHNWGDAPVKSLIHHWYWGRAEIGNF 224  Bacillus
OOS02358      152 PKSGHmyy-------gqKGNEKLFAWLPSVPQGaVLATIRVGDEtfRATGTGYHDHNWGNAPMASLIHHWYWGRAQVGDF 224  Moraxella cun...
SUB53115      148 PATGHwl--------fdEDHQKKFAWLLAVPCGrVTASLDIGGKveYLQGVGYHDHNWGTVAMSHLLDHWWWAHTTIGDR 219  Nocardia bras...
WP_094094517  158 PETGYfy--------fgDQEQHYFAWLPSVPEGrVEAEVKVEGKtlTYTGTGYHDHNWGNISMMSIIRHWYWGRAKIGRY 229  Paenibacillus...
PSR72288      181 AAGHSpcapassppgqsFELGPDFGWNNAVPDAdARVDLTINGSrlKFSGSGYHDKNWGSRPIETVVGSWYWGHARVGPL 260  Phlebia centr...
OBT70374      179 PCGPNeag-------qrLDVSPNVGWANAMPDAdAVVDFTVLGRplKFTGVGYHDKNWGDKPFTTHVSSWYWGHGRLGPY 251  Pseudogymnoas...
PNP78982      181 VCGPLeag-------qnMKVLPNVGWVNVMPAAdADVDVKINGQniKFTGNGYHDKNWGDAPFLASLNGWYWGHATLGDY 253  Fusarium nygamai
CVK95656      153 PGSGRw-----------DVEGRHFSWFVAVPGGqLIGDVTVNGTktRVEGNAYHDHNWGNVPMETILDNWLWGRADVGGL 221  Fusarium mang...
Feature 1                                                                                         
Q5AR52        940 SLVWFDVMGRDGKEYf--SAWITEAGNVILCGCEp--DSVLVRPWGENsgfppgrgTPAPSGYSLRYdlg--qnQIFIAN 1013 Aspergillus n...
WP_154073609  222 TVIASHITATAAYGYetqIVYMLAKDGQIIADDE---TKVAFDAERVEtd----gkTGKPVGDVTRYtyq-dtdTRYVVS 293  Bradyrhizobiu...
WP_147635443  235 TVIVCNITAEEQYGYkahPVFMLAKGNKILADDG---NLIRLTKRDEYld----dlTGKPVANTLVYeye-eedKHYTVT 306  Risungbinella...
WP_125715604  215 QVISTETTAEKAFGYqklPVMMIADHGELVSGDE---SNMTVEDSDTIid----ptTKKPVQQTLRYkvt-tpvADYVVT 286  Lactobacillus...
WP_048687006  225 TVIAANITAAEEFEYipqIVFMLAKDGEVVADDE---SAVTFSLAEMQvn----eaTGKPVGDIVKYetr-ngdNRYVVT 296  Bacillus
OOS02358      225 TVIASNITAAQQFEYipqTVFLLAKNGEILADDEr-cVKFSIEQIQVDe------tTRKPVADLVKYeti-ngnNRYVVT 296  Moraxella cun...
SUB53115      220 TVVVYDLITARKYGHi-qLSTVLIMDGTNVIDDEtieSTLSLYPQNSGa-------SAIPDNFTISYhq---gpARARVT 288  Nocardia bras...
WP_094094517  230 QTISSYISGEKKFGDnkfPIFMLAKDGEIIADDAv--SFLHFWEEDPYtd-----sQTGQTFYNRLLydyndgrQHYRVT 302  Paenibacillus...
PSR72288      261 SIVWFDFIAPDGTEK---PSSYVSSDGTLISSTCd--LAGSVRPTGVNa-------TFPPNSQSAPA-------SGFRVI 321  Phlebia centr...
OBT70374      252 SIVWFDVLGKDGTEY---FSSYAAKDGKIITAQCg--GGIKVRPTGHNseyppkitSGNPEGLHIDLdlg--eeGILRVD 324  Pseudogymnoas...
PNP78982      254 NIVFFDMLDKNNVEK---VGGYVLKDGQVVGNTCt--TGLRVRPVGTPypp--tlgSTNPTQMTLNMtlddgteLDALLT 326  Fusarium nygamai
CVK95656      222 SVVSASVRFRQDCGGrevPLLFVVRGNEVLVDVSd-dQLVCLEGVKIAqp-----dTGKPTSSDCIYlvksaggNLAQVR 295  Fusarium mang...
Feature 1                                                       
Q5AR52       1014 FTREVn--IIDNDFTKHIiglfsg-gfeggeQYEGRAMADQFQFGD 1056 Aspergillus nidulans FGSC A4
WP_154073609  294 FARQKt--ILQAIFTDRApllkri--iarliSFDGAYHRFTGKVTI 335  Bradyrhizobium erythrophlei
WP_147635443  307 FQREKd--ISKYRFIDTFrgakke--aaiaaGFDGAYLRFTGEVRL 348  Risungbinella massiliensis
WP_125715604  287 YAATKt--ISGFKMIDQLsgikkf--laklaGFDGAYLRFTGEISV 328  Lactobacillus sp. 54-2
WP_048687006  297 FEREKt--ILASKFIDQApaeqra--kleatGEDGAYLRFTGYVTI 338  Bacillus
OOS02358      297 FERTKt--ILASRFIDQAppdqra--klemsGEDGAYLRFTGLVTV 338  Moraxella cuniculi
SUB53115      289 FHRRRv--ILHRRLIDQLtgarrl--lakliASQGAYWRFTGTAEM 330  Nocardia brasiliensis
WP_094094517  303 YQRERd--IEFHQASASLskplql--lakviRLDGVYIRFTGMATI 344  Paenibacillus physcomitrellae
PSR72288      322 IPSQDgggLTFNITHKEVi-----------yDSPGQAVRWIGSVSG 356  Phlebia centrifuga
OBT70374      325 VKANT---VLADAFLFYRasgllsggvdggpQYTGVSLYDEFKFQF 367  Pseudogymnoascus sp. 23342-1-I1
PNP78982      327 VKKTQidiELYSRWIGSIdgtv------gdyKASGSALWEQFKVAS 366  Fusarium nygamai
CVK95656      296 FDGQQk-vIASFPYQTTSq------------EWETRYTRYAGMVTI 328  Fusarium mangiferae

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